Abstract:
:To be able to correlate real time membrane potential or ion flux changes with further downstream gene transcription responses due to extremely low frequency (ELF) electromagnetic field (EMF) exposure, we devised an experimental system consisting of a pair of symmetric circular coils. This system can be used on an inverted microscope stage (real time signaling) as well as inside controlled environment incubators (gene transcription end points). The system includes a unique, custom made switch box for blinding the experimental staff and a power amplifier. We report herein the design and characterization of the system with respect to parameters considered important in in vitro ELF-EMF exposure studies, including linear magnetic field distribution, compensation for microscope objective lens interference, heating effects of the coils, and harmonic content of the signals.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Rao RR,Kisaalita WSdoi
10.1002/bem.10151subject
Has Abstractpub_date
2004-01-01 00:00:00pages
27-32issue
1eissn
0197-8462issn
1521-186Xjournal_volume
25pub_type
杂志文章abstract::The extent of electromagnetic interference (EMI) from 16.7-Hz alternate current power lines in the human surface electrocardiogram (ECG) was evaluated. Results showed a direct linear correlation between mean EMI and magnetic induction of 5.8-21 microT on a railroad platform (electric field: 270 V/m). EMI inside a rail...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20319
更新日期:2007-07-01 00:00:00
abstract::We study the effect of microwaves at 2,450 MHz on protein unfolding using surface plasmon resonance sensing. Our experimental method makes use of the fact that unfolding proteins tend to bind to chaperones on their unfolding pathway and this attachment is readily monitored by surface plasmon resonance. We use the prot...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20382
更新日期:2008-05-01 00:00:00
abstract::In the last few years, significant developments have taken place in the field of Wireless Local Area Networks (WLAN), and the popularity of portable devices supporting Wireless Fidelity (Wi-Fi) is continuously growing. At the same time, the number of Active Implanted Medical Devices (AIMD) being placed in patients is ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20601
更新日期:2010-10-01 00:00:00
abstract::The use of electronic article surveillance (EAS) systems has become popular in many public sites. As a consequence, concern has risen about infant exposure to magnetic fields (MFs) from this kind of device. To evaluate infant exposure to MFs of an EAS system (operating at 125 kHz and 13.56 MHz), we numerically compare...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21901
更新日期:2015-04-01 00:00:00
abstract::The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10113
更新日期:2003-07-01 00:00:00
abstract::A biphasic modulation of responsiveness of spleen lymphocytes to mitogens was observed in mice exposed to 2,450-MHz radiation at power densities of 5-15 mW/cm2 over various periods ranging between one and 17 days. This modulated phenomenon may be explained on the basis of 1) suppression of lymphocyte response to micro...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010108
更新日期:1980-01-01 00:00:00
abstract::Our previous studies showed that extremely low frequency magnetic fields (ELF-MFs) inhibited tumor growth and change proportion of splenic regulatory T cells (Treg cells). Here, we focus on the effect of ELF-MFs on lung metastatic melanoma mouse model and the regulatory mechanism of ELF-MFs on the differentiation of T...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21954
更新日期:2016-02-01 00:00:00
abstract::There are large disparities between basic restrictions for exposure to extremely low-frequency (0-3 kHz) Electric and Magnetic Fields set by two major international bodies. Both bodies agree that these basic restrictions should prevent neuro-stimulatory effects: the retinal phosphene at frequencies up to a few hundred...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20412
更新日期:2008-09-01 00:00:00
abstract::Daily preexposure and postexposure mass measurements of 65 rats (young males and females, old males) a proprietary pulsed wound healing field, pulsed electromagnetic field, (PEMF), or their control fields for 4 h/day for 21 days. Statistical analysis of mass changes over time showed that young rats exposed to PEMF los...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.92
更新日期:2002-01-01 00:00:00
abstract::This is the first of two articles addressing the most appropriate crossover frequency at which incident power flux density (S(inc)) replaces the spatial peak value of the specific energy absorption rate (SAR) averaged over 1 or 10 g (i.e., peak 1 or 10 g SAR) as the basic restriction for protecting against radiofreque...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20578
更新日期:2010-09-01 00:00:00
abstract::This paper computationally verifies the effect of anesthesia on temperature variations in the rabbit eye due to microwave energy. The main reason for this investigation is that our previous paper suggested a reduction in blood flow due to the administration of anesthesia, resulting in an overestimated temperature incr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20251
更新日期:2006-12-01 00:00:00
abstract::Sprague-Dawley male rats, maintained in a 14:10 h light:dark cycle were exposed for 30 days (starting at 56 days of age) to a 65 kV/m, 60 Hz electric field or to a sham field for 20 h/day beginning at dark onset. Pineal N-acetyltransferase (NAT), hydroxy-indole-o-methyl transferase (HIOMT), and melatonin as well as se...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250150506
更新日期:1994-01-01 00:00:00
abstract::Treatment with direct electric current (DC) can inhibit tumor growth in several systems. To evaluate the cellular reactions generated by this treatment, we stimulated mouse mastocytoma P815 cells with DC and examined their viability and ultrastructural characteristics, as well as the effect of DC on surface carbohydra...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/1521-186x(200012)21:8<597::aid-bem6>3.0.co
更新日期:2000-12-01 00:00:00
abstract::The controversy over the possible association between magnetic field exposure and childhood leukemia has led several researchers to summarize the literature using meta-analysis. This paper reviews these previous meta-analyses and extends them by adding results from four studies published since the most recent analysis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,meta分析
doi:10.1002/1521-186x(2001)22:5+<::aid-bem1026>3.3.co;
更新日期:2001-01-01 00:00:00
abstract::The dielectric properties of the bovine brain grey and white matter in the frequency range from 20 kHz to 100 MHz were measured at different times following animal death. Changes in the dielectric parameters versus time are interpreted in terms of the reduction of the cell volume fraction that results from either cell...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070105
更新日期:1986-01-01 00:00:00
abstract::Recent work has demonstrated that the reflection coefficient of human skin in the frequency range from 95 to 110 GHz (W band) mirrors the temporal relaxation of stress induced by physical exercise. In this work, we extend these findings to show that in the event of a subtle trigger to stress, such as mental activity, ...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.21698
更新日期:2012-07-01 00:00:00
abstract::Most of the research concerning magnetic antinociception was focused on brief exposure less than 1 h. The main purpose of the present study was to determine the effect of extremely low frequency (ELF) magnetic field (MF) repeated exposures on rats in inducing antinociception and to find the effective analgesic "time w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20232
更新日期:2006-09-01 00:00:00
abstract::Introduction of genetic material into muscle tissue has been extensively researched, including isolation and in vitro expansion of primary myoblasts as a potential source of cells for skeletal and heart muscle tissue engineering applications. In this study, we optimized the electroporation protocol for introduction of...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21936
更新日期:2015-12-01 00:00:00
abstract::Cytostatic-induced polyneuropathy (CIPN) is a common and serious toxicity in tumor patients. Treatment and prophylactic measures are mainly ineffective. Therefore, there is an urgent need to establish a sufficient therapy for pPNP. Between July 2007 and August 2008, 20 patients were treated with low frequency (4-12 Hz...
journal_title:Bioelectromagnetics
pub_type: 信件
doi:10.1002/bem.21897
更新日期:2015-04-01 00:00:00
abstract::Electric fields induced in a conductive body by the magnetic field of a current-carrying wire were analyzed theoretically and experimentally to assess the dosimetric importance of highly nonuniform, field-exposure conditions. Experimentation revealed that a 60-Hz magnetic field was inversely proportional to the radius...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130305
更新日期:1992-01-01 00:00:00
abstract::Exposure systems that provide good magnetic field uniformity, minimum stray fields, and minimal heating, vibration, and hum, as well as capability for true sham exposure in which current flows in the coils, are needed to determine rigorously the biological effects of weak magnetic fields. Designs based on acrylic poly...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250150610
更新日期:1994-01-01 00:00:00
abstract::Adair [Bioelectromagnetics 14:1-4, 1993] writes that "the effects of 60 Hz magnetic fields of 5 microT (50 mG) or less on biological structures holding magnetite (Fe3O4) are shown to be much smaller than those from thermal agitation; hence such interactions cannot be expected to be biologically significant." This conc...
journal_title:Bioelectromagnetics
pub_type: 评论,杂志文章
doi:10.1002/bem.2250150308
更新日期:1994-01-01 00:00:00
abstract::Extremely-low-frequency (ELF) and very-low-frequency (VLF) magnetic fields as emitted by monochrome and multichrome video display units (VDUs) were measured for their frequency domains. The rms magnetic-flux densities (MFDs) were measured as a function of distance from all six sides of VDUs of four types. The MFDs at ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250120106
更新日期:1991-01-01 00:00:00
abstract::Childhood cancer has been modestly associated with wire codes, an exposure surrogate for power frequency magnetic fields, but less consistently with measured fields. We analyzed data on the population distribution of wire codes and their relationship with several measured magnetic field metrics. In a given geographic ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/(sici)1521-186x(1997)18:2<99::aid-bem2>3.0
更新日期:1997-01-01 00:00:00
abstract::Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca(2+)](c)) plays an important role. To better understand the biological conditions under which ELF M...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20248
更新日期:2006-07-01 00:00:00
abstract::Since 1994, our research has demonstrated how thermophysiological responses are mobilized in human volunteers exposed to three radio frequencies, 100, 450, and 2450 MHz. A significant gap in this frequency range is now filled by the present study, conducted at 220 MHz. Thermoregulatory responses of heat loss and heat ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20105
更新日期:2005-09-01 00:00:00
abstract::This report describes some experimental measurements of the internal field levels induced within isolated chick-forebrains irradiated at 50, 147, and 450 MHz, under essentially the same conditions as those used in the in vitro calcium-ion efflux experiments. Ratios of incident power at 50/147 MHz and 147/450 MHz that ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050302
更新日期:1984-01-01 00:00:00
abstract::We have previously employed the Lorentz-Langevin model to describe the effects of weak exogenous magnetic fields via the classical Lorentz force on a charged ion bound in a harmonic oscillator potential, in the presence of thermal noise forces. Previous analyses predicted that microT-range fields give rise to a rotati...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20494
更新日期:2009-09-01 00:00:00
abstract::The effects of millimeter microwaves in the frequency range of 54-76 GHz on capacitance and conductance of lipid bilayer membranes (BLM) were studied. Some of the membranes were modified by gramicidin A and amphotericin B or by tetraphenylboron anions (TPhB-). The millimeter microwaves were pulse-modulated (PW) at rep...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160209
更新日期:1995-01-01 00:00:00
abstract::We suggest an experimental comparison of two directions for applying the time-varying magnetic fields which have been found to speed spontaneous regeneration of injured peripheral nerves and in attempts to repair spinal cord injuries. Time-varying magnetic fields induce currents in a plane perpendicular to the magneti...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20331
更新日期:2007-09-01 00:00:00