Abstract:
:Treatment with direct electric current (DC) can inhibit tumor growth in several systems. To evaluate the cellular reactions generated by this treatment, we stimulated mouse mastocytoma P815 cells with DC and examined their viability and ultrastructural characteristics, as well as the effect of DC on surface carbohydrate expression. DC treatment affected cell viability and caused marked alterations in vital structures of P815 cells. Alterations varied depending on the duration of stimulation and polarity of electrode. Anodic and cathodic treatments caused decrease in cell viability, although the latter was more effective in generating cell lysis. DC stimulation also induced changes such as membrane damage, alterations in cell shape and chromatin organization, mitochondrial swelling and condensation, cytoplasmic swelling, and matrix rarefaction. Stimulation of P815 cells without contact with electrodes produced no alterations, suggesting that this contact might be essential for the occurrence of the cellular modifications. DC treatment also altered the membrane distribution of anionic sites of P815 cells, as well as the surface carbohydrate exposition, involving a diminished binding of Concanavalin A to the cell surface after cathodic stimulation, and an increased binding of sialic acid- and fucose-specific lectins after anodic treatment. In this work we describe important cellular targets for the action of DC, which may contribute to the understanding of the mechanisms by which DC supresses several kinds of tumors.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Veiga VF,Holandino C,Rodrigues ML,Capella MA,Menezes S,Alviano CSdoi
10.1002/1521-186x(200012)21:8<597::aid-bem6>3.0.cosubject
Has Abstractpub_date
2000-12-01 00:00:00pages
597-607issue
8eissn
0197-8462issn
1521-186Xpii
10.1002/1521-186X(200012)21:8<597::AID-BEM6>3.0.COjournal_volume
21pub_type
杂志文章abstract::In May 2011, the International Agency on Cancer in Research (IARC) issued an official statement concluding that cell phone usage was "possibly carcinogenic to humans." There have been considerable doubts that non-experts and experts alike fully understood what IARC's categorization actually meant, as "possibly carcino...
journal_title:Bioelectromagnetics
pub_type: 信件
doi:10.1002/bem.21851
更新日期:2014-07-01 00:00:00
abstract::A new method for finding the dielectric parameters of biological substances is presented. The method makes use of the loss-tangent as a function of frequency to identify the dominating relaxation process. The method was tested for a few cell suspensions (blood and lymphocytes) and two tissues (liver and spleen). The o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070303
更新日期:1986-01-01 00:00:00
abstract::A system is described that uses an oscillating magnetic field to produce power-frequency electric fields with strengths in excess of those produced in an animal or human standing under a high-voltage electric-power transmission line. In contrast to other types of exposure systems capable of generating fields of this s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050202
更新日期:1984-01-01 00:00:00
abstract::The controversy over the possible association between magnetic field exposure and childhood leukemia has led several researchers to summarize the literature using meta-analysis. This paper reviews these previous meta-analyses and extends them by adding results from four studies published since the most recent analysis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,meta分析
doi:10.1002/1521-186x(2001)22:5+<::aid-bem1026>3.3.co;
更新日期:2001-01-01 00:00:00
abstract::Stress proteins are important in protection during cardiac ischemia/reperfusion (cessation and return of blood flow) and are reportedly induced by electromagnetic (EM) fields. This suggests a possible ischemia protection role for EM exposures. To test this, chick embryos (96 h) were exposed to 60 Hz magnetic fields pr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1998)19:8<498::aid-bem8>3.
更新日期:1998-01-01 00:00:00
abstract::The extent of electromagnetic interference (EMI) from 16.7-Hz alternate current power lines in the human surface electrocardiogram (ECG) was evaluated. Results showed a direct linear correlation between mean EMI and magnetic induction of 5.8-21 microT on a railroad platform (electric field: 270 V/m). EMI inside a rail...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20319
更新日期:2007-07-01 00:00:00
abstract::Heat shock proteins (HSPs) are rapidly induced by a variety of stressors, including heat shock, ethanol, heavy metals, UV, and gamma-radiation. Mitogen-activated protein kinases (MAPKs) are also involved in the stress transduction pathways in all eukaryotes. In this study, we attempted to determine whether radiofreque...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20235
更新日期:2006-10-01 00:00:00
abstract::The goal of the research reported here is to narrow the range of uncertainty about peripheral nerve stimulation (PNS) thresholds associated with whole body magnetic field exposures at 50/60 Hz. This involved combining PNS thresholds measured in human subjects exposed to pulsed magnetic gradient fields with calculation...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20106
更新日期:2005-09-01 00:00:00
abstract::The impedance spectroscopy technique (IST) was used for studying the effect of a 0.5 T magnetic field on the electrical properties of whole human blood. A Solartron SI 1260 spectrometer was used to measure the impedance spectra of magnetic field exposed blood samples compared to non-exposed samples. An equivalent elec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20132
更新日期:2005-10-01 00:00:00
abstract::Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) at 0.2 mT. In the present study, w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20207
更新日期:2006-05-01 00:00:00
abstract::We conducted a pilot study to assess magnetic field levels in electric compared to gasoline-powered vehicles, and established a methodology that would provide valid data for further assessments. The sample consisted of 14 vehicles, all manufactured between January 2000 and April 2009; 6 were gasoline-powered vehicles ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21730
更新日期:2013-02-01 00:00:00
abstract::In view of possible therapeutic applications of magnetic fields, the effect of an enhancement of neuronal outgrowth at higher figures of flux density and induced field strength was investigated. On the average sinusoidal magnetic field treatment at 100 microTrms/50 Hz did not change nerve growth factor (NGF) induced n...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20073
更新日期:2005-01-01 00:00:00
abstract::This paper computationally verifies the effect of anesthesia on temperature variations in the rabbit eye due to microwave energy. The main reason for this investigation is that our previous paper suggested a reduction in blood flow due to the administration of anesthesia, resulting in an overestimated temperature incr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20251
更新日期:2006-12-01 00:00:00
abstract::This is the first of two articles addressing the most appropriate crossover frequency at which incident power flux density (S(inc)) replaces the spatial peak value of the specific energy absorption rate (SAR) averaged over 1 or 10 g (i.e., peak 1 or 10 g SAR) as the basic restriction for protecting against radiofreque...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20578
更新日期:2010-09-01 00:00:00
abstract::Atomic force microscopy (AFM), transmission electron microscopy (TEM), and confocal laser scanning microscopy were used to investigate the effects of a 50 Hz 0.4 mT magnetic field (MF) on the clustering of purified epidermal growth factor receptors (EGFRs) and EGFRs in Chinese hamster lung (CHL) cell membrane. The res...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20293
更新日期:2007-04-01 00:00:00
abstract::Electric fields induced in a conductive body by the magnetic field of a current-carrying wire were analyzed theoretically and experimentally to assess the dosimetric importance of highly nonuniform, field-exposure conditions. Experimentation revealed that a 60-Hz magnetic field was inversely proportional to the radius...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130305
更新日期:1992-01-01 00:00:00
abstract::One hundred subjects, males and females with ages ranging between 18 and 48 years, were studied under both field-exposed and sham-exposed conditions. A 50 Hz, 100 mu T magnetic field (MF) was used. To examine the effect of field exposure on performance, a two-alternative, forced-choice, duration-discrimination task wi...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/(SICI)1521-186X(1996)17:2<131::AID-BEM7>3.
更新日期:1996-01-01 00:00:00
abstract::The electromagnetic exposure of cell phone users depends on several parameters. One of the most dominant of these is the distance between the cell phone and the head tissue. The pinna can be regarded as a spacer between the top of the phone and the head tissue. The size of this spacer has not yet been systematically s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20575
更新日期:2010-07-01 00:00:00
abstract::In-vitro studies of biological effects of electromagnetic fields are often conducted with cultured cells either in suspension or grown in a monolayer. In the former case, the exposed medium can be assumed to be homogeneous; however, eventually the cells settle to the bottom of the container forming a two layer system ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140106
更新日期:1993-01-01 00:00:00
abstract::B lymphocytes collected from normal ICR Swiss mouse spleens were exposed in vitro in a Crawford cell to 147-MHz radiofrequency (RF) radiation, amplitude modulated by a 9-, 16-, or 60-Hz sine wave. The power densities ranged between 0.11 and 48 mW/cm2. The irradiated samples and the controls were maintained at 37 degre...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250040206
更新日期:1983-01-01 00:00:00
abstract::Conductive nanoparticles may enhance tissue heating during radiofrequency (RF) irradiation. Specific absorption rate (SAR) is known to rise with the electrical conductivity of tissue. However, no studies to date have measured the relationship between complex permittivity and nanoparticle concentration in tissue-like s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20595
更新日期:2010-12-01 00:00:00
abstract::Studies have evaluated the electroencephalography (EEG) of humans and laboratory animals during and after Radiofrequency (RF) exposures. Effects of RF exposure on the blood-brain barrier (BBB) have been generally accepted for exposures that are thermalizing. Low level exposures that report alterations of the BBB remai...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/bem.10179
更新日期:2003-01-01 00:00:00
abstract::The present study investigates the effects of a weak (+/-200 microT(pk)), pulsed, extremely low frequency magnetic field (ELF MF) upon the human electroencephalogram (EEG). We have previously determined that exposure to pulsed ELF MFs can affect the EEG, notably the alpha frequency (8-13 Hz) over the occipital-parieta...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20434
更新日期:2009-01-01 00:00:00
abstract::The wide and growing use of cellular phones has raised questions about the possible health risks associated with radio frequency (RF) electromagnetic fields. It would be helpful for epidemiologists as well as cellular phone users to obtain the relative exposure levels, because the RF exposure level is very difficult t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20203
更新日期:2006-09-01 00:00:00
abstract::Pulsed electromagnetic fields (PEMF) have been proven to be effective for promoting bone mass and regulating bone turnover both experimentally and clinically. However, the exact mechanisms for the regulation of PEMF on osteoclastogenesis as well as optical exposure parameters of PEMF on inhibiting osteoclastic activit...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22070
更新日期:2017-12-01 00:00:00
abstract::The US National Electrical Code's (NEC) requirement to ground a home's electrical service to the residential water line results in a voltage between the water line and earth, V W-E. The voltage may result from ground return current that flows into the earth via the water line or from inductive effects from other sourc...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20033
更新日期:2004-10-01 00:00:00
abstract::The effects of millimeter microwaves in the frequency range of 54-76 GHz on capacitance and conductance of lipid bilayer membranes (BLM) were studied. Some of the membranes were modified by gramicidin A and amphotericin B or by tetraphenylboron anions (TPhB-). The millimeter microwaves were pulse-modulated (PW) at rep...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160209
更新日期:1995-01-01 00:00:00
abstract::Experiments were conducted to see whether the cellular response to electromagnetic (EM) fields occurs through a detection process involving temporal sensing. L929 cells were exposed to 60 Hz magnetic fields and the enhancement of ornithine decarboxylase (ODC) activity was measured to determine cellular response to the...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:5<388::aid-bem6>3.
更新日期:1997-01-01 00:00:00
abstract::We have shown that 50 Hz sinusoidal magnetic fields within the 5-10 microTesla (microT) rms range cause an intensity-dependent reduction in nerve growth factor (NGF) stimulation of neurite outgrowth (NO) in PC-12 cells. Here we report on the frequency dependence of this response over the 15-70 Hz range at 5 Hz interva...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160607
更新日期:1995-01-01 00:00:00
abstract::Daily preexposure and postexposure mass measurements of 65 rats (young males and females, old males) a proprietary pulsed wound healing field, pulsed electromagnetic field, (PEMF), or their control fields for 4 h/day for 21 days. Statistical analysis of mass changes over time showed that young rats exposed to PEMF los...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.92
更新日期:2002-01-01 00:00:00