Cellular damage and altered carbohydrate expression in P815 tumor cells induced by direct electric current: an in vitro analysis.

Abstract:

:Treatment with direct electric current (DC) can inhibit tumor growth in several systems. To evaluate the cellular reactions generated by this treatment, we stimulated mouse mastocytoma P815 cells with DC and examined their viability and ultrastructural characteristics, as well as the effect of DC on surface carbohydrate expression. DC treatment affected cell viability and caused marked alterations in vital structures of P815 cells. Alterations varied depending on the duration of stimulation and polarity of electrode. Anodic and cathodic treatments caused decrease in cell viability, although the latter was more effective in generating cell lysis. DC stimulation also induced changes such as membrane damage, alterations in cell shape and chromatin organization, mitochondrial swelling and condensation, cytoplasmic swelling, and matrix rarefaction. Stimulation of P815 cells without contact with electrodes produced no alterations, suggesting that this contact might be essential for the occurrence of the cellular modifications. DC treatment also altered the membrane distribution of anionic sites of P815 cells, as well as the surface carbohydrate exposition, involving a diminished binding of Concanavalin A to the cell surface after cathodic stimulation, and an increased binding of sialic acid- and fucose-specific lectins after anodic treatment. In this work we describe important cellular targets for the action of DC, which may contribute to the understanding of the mechanisms by which DC supresses several kinds of tumors.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Veiga VF,Holandino C,Rodrigues ML,Capella MA,Menezes S,Alviano CS

doi

10.1002/1521-186x(200012)21:8<597::aid-bem6>3.0.co

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

597-607

issue

8

eissn

0197-8462

issn

1521-186X

pii

10.1002/1521-186X(200012)21:8<597::AID-BEM6>3.0.CO

journal_volume

21

pub_type

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