Abstract:
:Heat shock proteins (HSPs) are rapidly induced by a variety of stressors, including heat shock, ethanol, heavy metals, UV, and gamma-radiation. Mitogen-activated protein kinases (MAPKs) are also involved in the stress transduction pathways in all eukaryotes. In this study, we attempted to determine whether radiofrequency (RF) radiation is able to induce a non-thermal stress response. Human T-lymphocyte Jurkat cells and rat primary astrocytes were exposed to 1763 MHz of RF radiation at an average specific absorption rate (SAR) of either 2 W/kg or 20 W/kg, for 30 min or 1 h. Temperature was completely controlled at 37 +/- 0.2 degrees C throughout the exposure period. The sham exposures were performed under exactly identical experimental conditions without exposure to RF radiation. We assessed alterations in the expression of HSPs and the activation of MAPKs in the RF-exposed cells. No detectable difference was observed in the expression levels of HSP90, HSP70, and HSP27. The phosphorylation status of MAPKs, extracellular signal-regulated kinases (ERK1/2), c-Jun N-terminal protein kinases (JNK1/2), or p38, did not change significantly. In order to determine whether RF radiation can promote the effects of 12-O-tetradecanoylphorbol 13-acetate (TPA) on stress response, cells were exposed to RF radiation coupled with TPA treatment. When TPA alone was applied, the MAPKs were found to be phosphorylated in a dose-dependent manner. However, RF radiation did not result in any enhancement of TPA-induced MAPK phosphorylation. Neither TPA nor RF radiation exerted any detectable effect on the induction of HSPs. These results indicate that 1763 MHz RF radiation alone did not elicit any stress response, nor did it have any effect on TPA-induced MAPK phosphorylation, under our experimental conditions.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Lee JS,Huang TQ,Kim TH,Kim JY,Kim HJ,Pack JK,Seo JSdoi
10.1002/bem.20235subject
Has Abstractpub_date
2006-10-01 00:00:00pages
578-88issue
7eissn
0197-8462issn
1521-186Xjournal_volume
27pub_type
杂志文章abstract::To evaluate duration- and dose-dependent effects of continuous exposure to a 60 Hz magnetic field (MF) on the testes in mice, BALB/c male mice were exposed to a 60 Hz MF at 100 μT for 24 h a day for 2, 4, 6, or 8 weeks, and at 2, 20, or 200 μT for 24 h a day for 8 weeks. Any exposures to MF did not significantly affec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20412
更新日期:2008-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20270
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20248
更新日期:2006-07-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10045
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10070
更新日期:2003-02-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20262
更新日期:2007-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 历史文章,杂志文章
doi:10.1002/(sici)1521-186x(1999)20:4+<9::aid-bem3>3.3
更新日期:1999-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:1<28::aid-bem6>3.0
更新日期:1997-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.92
更新日期:2002-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20390
更新日期:2008-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20077
更新日期:2005-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
doi:10.1002/(sici)1521-186x(1999)20:4+<110::aid-bem13>
更新日期:1999-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050406
更新日期:1984-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22003
更新日期:2016-12-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Bioelectromagnetics
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更新日期:2010-10-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050109
更新日期:1984-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20322
更新日期:2007-09-01 00:00:00
abstract::A biphasic modulation of responsiveness of spleen lymphocytes to mitogens was observed in mice exposed to 2,450-MHz radiation at power densities of 5-15 mW/cm2 over various periods ranging between one and 17 days. This modulated phenomenon may be explained on the basis of 1) suppression of lymphocyte response to micro...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010108
更新日期:1980-01-01 00:00:00
abstract::Reports that low-intensity microwave radiation induces heat-shock reporter gene expression in the nematode, Caenorhabditis elegans, have recently been reinterpreted as a subtle thermal effect caused by slight heating. This study used a microwave exposure system (1.0 GHz, 0.5 W power input; SAR 0.9-3 mW kg(-1) for 6-we...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20515
更新日期:2009-12-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20438
更新日期:2009-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070105
更新日期:1986-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20641
更新日期:2011-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20379
更新日期:2008-04-01 00:00:00