Abstract:
:Our previous studies showed that extremely low frequency magnetic fields (ELF-MFs) inhibited tumor growth and change proportion of splenic regulatory T cells (Treg cells). Here, we focus on the effect of ELF-MFs on lung metastatic melanoma mouse model and the regulatory mechanism of ELF-MFs on the differentiation of Treg cells. Tumor-bearing mice were exposed to sham ELF-MFs and ELF-MFs (0.4 T, 7.5 Hz) 2 h/day for 27 days. Metastatic tumor burden of lung was significantly decreased after ELF-MF treatment. Compared to the control group, expressions of matrix metalloproteinase (MMP2, MMP9) and forkhead box P3 (Foxp3) in lung nodules significantly decreased in the ELF-MF group. Moreover, in vitro, after stimulated with anti-CD3, anti-CD28 antibodies and transforming growth factor-β (TGF-β) and treated with ELF-MFs for 2 h, expression of Foxp3 in total T cells was significantly decreased. Differentiation rate of Treg cells was inhibited from 32.0% to 22.1% by ELF-MFs. Furthermore, reactive oxygen species (ROS) was increased and phospho-serine/threonine protein kinase (p-AKT) was inhibited in both T cells and Jurkat cells. ROS scavenger N-acetyl-l-cysteine reversed inhibition of AKT pathway and expression of Foxp3 from 18.6% to 26.6% in T cells. Taken together, our data show that ELF-MF exposure promoted the inhibitory effect of ROS on AKT pathway and decreased Foxp3 expression, which provides an explanation for why ELF-MF exposure can inhibit differentiation of Treg cells and enhance antitumor effect in metastatic melanoma mouse model.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Tang R,Xu Y,Ma F,Ren J,Shen S,Du Y,Hou Y,Wang Tdoi
10.1002/bem.21954subject
Has Abstractpub_date
2016-02-01 00:00:00pages
89-98issue
2eissn
0197-8462issn
1521-186Xjournal_volume
37pub_type
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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abstract::This paper traces the history of the development of the Radiofrequency Radiation Dosimetry Handbook and its subsequent impact on radio frequency radiation exposure standards. The author's recollections are used to illustrate the behind the scenes efforts of the individuals involved in this project. The development of ...
journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
pub_type: 信件
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Bioelectromagnetics
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更新日期:2002-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250060209
更新日期:1985-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20232
更新日期:2006-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250030108
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journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010203
更新日期:1980-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250120106
更新日期:1991-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050406
更新日期:1984-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250150406
更新日期:1994-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20020
更新日期:2004-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
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更新日期:1992-01-01 00:00:00