Abstract:
:Introduction of genetic material into muscle tissue has been extensively researched, including isolation and in vitro expansion of primary myoblasts as a potential source of cells for skeletal and heart muscle tissue engineering applications. In this study, we optimized the electroporation protocol for introduction of short interfering ribonucleic acid (siRNA) against messenger RNA for Hypoxia Inducible Factor 1α (HIF-1α) into cultured primary human myoblasts. We established optimal pulsing protocol for siRNA electro transfection, and theoretically analyzed the effect of electric field and pulse duration on silencing efficiency and electrophoretic displacement of siRNA. Silencing of HIF-1α was determined with quantitative polymerase chain reaction and Western Blot. The most efficient silencing (71% knockdown) was achieved with 8 × 2 ms pulses, E = 0.6 kV/cm. Viability was determined immediately, 1 h and 48 h after electroporation. In general, there was a trade-off between efficient silencing and preserved viability. Electric field and pulse duration are crucial parameters for silencing, since both increase membrane permeabilization and electrophoretic transfer of siRNA. Short-term viability showed immediate toxicity of pulses due to membrane damage, while indirect effects on cell proliferation were observed after 48 h. Presented results are important for faster optimization of electroporation parameters for ex vivo electrotransfer of short RNA molecules into primary human myoblasts.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Lojk J,Mis K,Pirkmajer S,Pavlin Mdoi
10.1002/bem.21936subject
Has Abstractpub_date
2015-12-01 00:00:00pages
551-63issue
8eissn
0197-8462issn
1521-186Xjournal_volume
36pub_type
杂志文章abstract::Accumulating evidence does not yet confirm the effect of power line frequency magnetic field (MF) on human health and fertility. We recently reported that, at continuous 60 Hz MF exposure in mice, the dose given as magnetic flux density (tesla; T) and duration of exposure was related to induce testicular germ cell apo...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22146
更新日期:2018-10-01 00:00:00
abstract::The hypogeomagnetic field (HGMF; magnetic fields <200 nT) is one of the fundamental environmental factors of space. However, the effect of HGMF exposure on living systems remains unclear. In this article, we examine the biological effects of HGMF on the embryonic development of Xenopus laevis (African clawed frog). A ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20699
更新日期:2012-04-01 00:00:00
abstract::The constant exposure of modern society to extremely low frequency magnetic fields (ELF-MF) has raised considerable concerns about the potential risks to male reproduction. However, the epidemiological and experimental data remain contradictory and inconclusive. In the present study, we investigated the effects of 50 ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21816
更新日期:2014-01-01 00:00:00
abstract::A biphasic modulation of responsiveness of spleen lymphocytes to mitogens was observed in mice exposed to 2,450-MHz radiation at power densities of 5-15 mW/cm2 over various periods ranging between one and 17 days. This modulated phenomenon may be explained on the basis of 1) suppression of lymphocyte response to micro...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250010108
更新日期:1980-01-01 00:00:00
abstract::Most of the research concerning magnetic antinociception was focused on brief exposure less than 1 h. The main purpose of the present study was to determine the effect of extremely low frequency (ELF) magnetic field (MF) repeated exposures on rats in inducing antinociception and to find the effective analgesic "time w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20232
更新日期:2006-09-01 00:00:00
abstract::In this study, we measured and analyzed the spectral characteristics of a low-frequency magnetic field (MF) inside several gasoline-powered cars while driving on busy city roads. The spectra obtained upon measurements in the interior of the cars are compared with those measured in office locations at different times o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22269
更新日期:2020-07-01 00:00:00
abstract::Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca(2+)](c)) plays an important role. To better understand the biological conditions under which ELF M...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20248
更新日期:2006-07-01 00:00:00
abstract::An attempt has been made to understand whether 50 Hz electric and magnetic fields (EMFs) are involved in producing bioeffects by exposing human erythrocytes in vitro. The study evaluated some key glycolytic enzymes, glucose consumption, lactate production, energy charge, 2,3-diphosphoglycerate, and reduced glutathione...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140410
更新日期:1993-01-01 00:00:00
abstract::Composition and content of lipids were studied in leaves of red perilla plants (Perilla nankinensis [Lour.] Decne.) grown in weak permanent horizontal magnetic field (PMF) of 500 µT flux density under controlled illumination, temperature, and humidity in the phytothron chamber. Control plants were grown under similar ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21957
更新日期:2016-02-01 00:00:00
abstract::Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therap...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22038
更新日期:2017-05-01 00:00:00
abstract::Pregnant squirrel monkeys were exposed to 2450-MHz (CW) microwaves at an equivalent power density of 10 mW/cm2 (SAR 3.4 mW/g) for three hours daily in a cavity-cage module. The exposure began when pregnancy was determined by a hormonal method, and continued through of offspring's first 9.5 months. After irradiation, t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250020305
更新日期:1981-01-01 00:00:00
abstract::We studied the effect produced on the development and functional activity of skeletal muscle cells from newborn Wistar rats in primary culture by weak static magnetic fields (WSMF; 60-400 µT) with a high capacity of penetrating the biological media. To reduce the impact of external magnetic fields, cells were cultured...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21876
更新日期:2014-12-01 00:00:00
abstract::The accurate and valid measurement of personal exposure to magnetic fields poses a major challenge for epidemiologic studies. When considering the various methods to assess exposure, it is unclear which measures are most relevant for studies of human disease, if any. Given these uncertainties, the Electromagnetic Fiel...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::A mechanism is presented that predicts new biological effects of static and sinusoidal weak magnetic fields. The model is based on an earlier proposed interference mechanism of quantum states of ions within protein cavities. The quantum dynamics of an ion is studied for the case of ion-protein complexes that rotate in...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::In this study, we aimed to clarify the effects of chronically applied static magnetic field (200 Gauss) on specific ATPase activities and bioelectrical and biomechanical responses in isolated rat diaphragm muscle. The mean activities of Na(+)-K+ ATPase and Ca2+ ATPase determined from the diaphragm homogenates were sig...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160302
更新日期:1995-01-01 00:00:00
abstract::This study analyzes if an external magnetic stimulus (2 kHz and approximately 0.1 μT applied near frontal cortex) influences working memory, perception, binary decision, motor execution, and sustained attention in humans. A magnetic stimulus and a sham stimulus were applied to both sides of the head (frontal cortex cl...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21944
更新日期:2016-01-01 00:00:00
abstract::In this work we tested viability, proliferation, and vulnerability of neural cells, after continuous radiofrequency (RF) electromagnetic fields exposure (global system for mobile telecommunications (GSM) modulated 900 MHz signal at a specific absorption rate (SAR) of 1 W/kg and maximum duration 144 h) generated by tra...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20507
更新日期:2009-10-01 00:00:00
abstract::In the clinic, although several pharmacological agents or surgical procedures are used to treat diabetes and diabetes-induced neuropathic pain, their success has been limited. Therefore, development of different alternatives in treatments is very important. The purpose of this study was to determine the efficacy of pu...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20524
更新日期:2010-01-01 00:00:00
abstract::This study examined whether 60 Hz magnetic field (MF) exposure alters intracellular calcium levels ([Ca(2+)](i)) in isolated bovine adrenal chromaffin cells, a classic model of neural responses. [Ca(2+)](i) was monitored by fluorescence video imaging of cells loaded with the calcium indicator fluo-4 during exposures t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10045
更新日期:2002-12-01 00:00:00
abstract::The objective of the study was to investigate the influence of humidity on analyzing electric field exposure using extremely low frequency (ELF) electric field measurements. The study included 322 measurements in a climate room. We used two commercial three-axis meters, EFA-3 and EFA-300, and employed two measurement ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21776
更新日期:2013-07-01 00:00:00
abstract::The dielectric behavior of the aqueous solutions of three widely differing macromolecules has been investigated: myoglobin, polyvinylpyrrolidone (PVP), and human serum low-density lipoprotein (LDL). It was not possible to interpret unambiguously the dielectric properties of the PVP solution in terms of water structure...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070206
更新日期:1986-01-01 00:00:00
abstract::We have exposed cultured bone cells to a pulsed electromagnetic field (PEMF) for different times to find the minimal exposure time necessary to stimulate an increase of DNA synthesis. We used two different human osteosarcoma cell lines, TE-85 and MG-63, and human normal osteoblast cell (NHOC) obtained from surgical bo...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1999)20:3<177::aid-bem4>3.
更新日期:1999-01-01 00:00:00
abstract::This paper presents a theoretical analysis of electromagnetic environments in power substations in service. A new analytical method is developed and applied to magnetic field calculations in 187-kV and 66-kV substations. Experimental results verify that the method offers a good prediction of the magnetic field profile...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250100106
更新日期:1989-01-01 00:00:00
abstract::The wide and growing use of cellular phones has raised questions about the possible health risks associated with radio frequency (RF) electromagnetic fields. It would be helpful for epidemiologists as well as cellular phone users to obtain the relative exposure levels, because the RF exposure level is very difficult t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20203
更新日期:2006-09-01 00:00:00
abstract::No causal treatment for chemotherapy-induced peripheral neuropathy (CIPN) is known. Therefore, there is an urgent need to develop a therapy for CIPN. Only scarce clinical data are available concerning magnetic field therapy (MFT) in this context. We conducted a unicentric, randomized, double-blind, placebo-controlled ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,随机对照试验
doi:10.1002/bem.22005
更新日期:2017-02-01 00:00:00
abstract::The current through the residential grounding circuit is an important source for magnetic fields; field variations near the grounding circuit accurately track fluctuations in this ground current. In this paper, a model is presented which permits calculation of the range of these fluctuations. A discrete network model ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250150109
更新日期:1994-01-01 00:00:00
abstract::We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(200002)21:2<100::aid-bem4>
更新日期:2000-02-01 00:00:00
abstract::Millimeter wave treatment (MMWT) is based on the systemic biological effects that develop following local skin exposure to low power electromagnetic waves in the millimeter range. In the present set of experiments, the hypoalgesic effect of this treatment was analyzed in mice. The murine nose area was exposed to MMW o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20389
更新日期:2008-05-01 00:00:00
abstract::We describe devices designed for magnetic field exposures in which field amplitude and gradients are controlled simultaneously. Dosimetry based on field continuation of high resolution magnetic field scans and numerical models is compared with validation measurements. The dosimetry variables we consider are based on t...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20098
更新日期:2005-05-01 00:00:00
abstract::An automated blood sampling system was developed for use with tethered baboons (Papio cynocephalus) during concurrent exposure to 60 Hz 30 kV/m electric fields and 0.1 mT (1.0 G) magnetic fields. The system was controlled by a FORTH-based microcomputer, which operated a pump, a fraction collector, and two pinch valves...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160710
更新日期:1995-01-01 00:00:00