Abstract:
:We have exposed cultured bone cells to a pulsed electromagnetic field (PEMF) for different times to find the minimal exposure time necessary to stimulate an increase of DNA synthesis. We used two different human osteosarcoma cell lines, TE-85 and MG-63, and human normal osteoblast cell (NHOC) obtained from surgical bone specimens. The cells were placed in multiwell plates and set in a tissue culture incubator between a pair of Helmoltz coils powered by a pulse generator (1.3-ms pulse, repeated at 75 Hz) for different periods of time. [3H]Thymidine incorporation was used to evaluate cell proliferation. The two osteosarcoma cell lines increase their thymidine incorporation when exposed to a PEMF for at least 30 min, both in a medium containing 10% fetal calf serum and in a serum-free medium. NHOC are known to increase their cell proliferation when exposed to PEMF but only if cultured in the presence of 10% fetal calf serum. In this experimental condition, three of the four cell lineages studied required at least 9 h of PEMF exposure to increase their DNA synthesis, whereas one cell lineage increased its cell proliferation after 6 h of PEMF exposure. Our observations confirm the hypothesis that the proliferative responses of NHOC and human osteosarcoma cell lines to PEMF exposure are quite different. Moreover, NHOC required minimal exposure times to PEMF to increase their cell proliferation, similar to that needed to stimulate bone formation in vivo.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
De Mattei M,Caruso A,Traina GC,Pezzetti F,Baroni T,Sollazzo Vdoi
10.1002/(sici)1521-186x(1999)20:3<177::aid-bem4>3.subject
Has Abstractpub_date
1999-01-01 00:00:00pages
177-82issue
3eissn
0197-8462issn
1521-186Xpii
10.1002/(SICI)1521-186X(1999)20:3<177::AID-BEM4>3.journal_volume
20pub_type
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