Effects of exposure to a 1950 MHz radio frequency field on expression of Hsp70 and Hsp27 in human glioma cells.

Abstract:

:Human glioma MO54 cells were used to investigate whether radio frequency (RF) field exposure could activate stress response genes. Cells were exposed to continuous wave 1950 MHz or sham conditions for up to 2 h. Specific absorption rates (SARs) were 1, 2, and 10 W/kg. For the cell growth experiment, cell numbers were counted at 0-4 days after exposure. Expression of Hsp27 and Hsp70, as well as the level of phosphorylated Hsp27 (78Ser) protein, was determined by Western blotting. It was found that sham exposed and RF exposed cells demonstrated a similar growth pattern up to 4 days after RF field exposure. RF field exposure at both 2 and 10 W/kg did not affect the growth of MO54 cells. In addition, there were no significant differences in protein expression of Hsp27 and Hsp70 between sham exposed and RF exposed cells at a SAR of 1, 2, or 10 W/kg for 1 and 2 h. However, exposure to RF field at a SAR of 10 W/kg for 1 and 2 h decreased the protein level of phosphorylated Hsp27 (78Ser) significantly. Our results suggest that although exposure to a 1950 MHz RF field has no effect on cell proliferation and expression of Hsp 27 and Hsp70, it may inhibit the phosphorylation of Hsp27 at Serine 78 in MO54 cells.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Miyakoshi J,Takemasa K,Takashima Y,Ding GR,Hirose H,Koyama S

doi

10.1002/bem.20077

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

251-7

issue

4

eissn

0197-8462

issn

1521-186X

journal_volume

26

pub_type

杂志文章
  • Determination of the induced ELF electric field distribution in a two layer in vitro system simulating biological cells in nutrient solution.

    abstract::In-vitro studies of biological effects of electromagnetic fields are often conducted with cultured cells either in suspension or grown in a monolayer. In the former case, the exposed medium can be assumed to be homogeneous; however, eventually the cells settle to the bottom of the container forming a two layer system ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250140106

    authors: Wang W,Litovitz TA,Penafiel LM,Meister R

    更新日期:1993-01-01 00:00:00

  • Continuous exposure to 60 Hz magnetic fields induces duration- and dose-dependent apoptosis of testicular germ cells.

    abstract::To evaluate duration- and dose-dependent effects of continuous exposure to a 60 Hz magnetic field (MF) on the testes in mice, BALB/c male mice were exposed to a 60 Hz MF at 100 μT for 24 h a day for 2, 4, 6, or 8 weeks, and at 2, 20, or 200 μT for 24 h a day for 8 weeks. Any exposures to MF did not significantly affec...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.21819

    authors: Kim HS,Park BJ,Jang HJ,Ipper NS,Kim SH,Kim YJ,Jeon SH,Lee KS,Lee SK,Kim N,Ju YJ,Gimm YM,Kim YW

    更新日期:2014-02-01 00:00:00

  • A model for radiofrequency electromagnetic field predictions at outdoor and indoor locations in the context of epidemiological research.

    abstract::We present a geospatial model to predict the radiofrequency electromagnetic field from fixed site transmitters for use in epidemiological exposure assessment. The proposed model extends an existing model toward the prediction of indoor exposure, that is, at the homes of potential study participants. The model is based...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20552

    authors: Bürgi A,Frei P,Theis G,Mohler E,Braun-Fahrländer C,Fröhlich J,Neubauer G,Egger M,Röösli M

    更新日期:2010-04-01 00:00:00

  • Occupational exposure levels of static magnetic field during routine MRI examination in 3T MR system.

    abstract::Occupational exposure to the high static magnetic fields (SMFs) during magnetic resonance imaging (MRI) examinations raises concerns of adverse health effects. In this study, personal exposure monitoring of the magnetic fields during routine examinations in two 3 T MRI systems was carried out. A three-axis Hall magnet...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,多中心研究

    doi:10.1002/bem.21817

    authors: Yamaguchi-Sekino S,Nakai T,Imai S,Izawa S,Okuno T

    更新日期:2014-01-01 00:00:00

  • Residential EMF exposure and childhood leukemia: meta-analysis and population attributable risk.

    abstract::The controversy over the possible association between magnetic field exposure and childhood leukemia has led several researchers to summarize the literature using meta-analysis. This paper reviews these previous meta-analyses and extends them by adding results from four studies published since the most recent analysis...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,meta分析

    doi:10.1002/1521-186x(2001)22:5+<::aid-bem1026>3.3.co;

    authors: Wartenberg D

    更新日期:2001-01-01 00:00:00

  • Local tissue temperature increase of a generic implant compared to the basic restrictions defined in safety guidelines.

    abstract::The objective of this study was to investigate if persons with implantable medical devices are intrinsically protected by the current electromagnetic safety guidelines. For inter-laboratory comparisons, the U.S. Food and Drug Administration has defined a generic implant as consisting of an insulated wire with noninsul...

    journal_title:Bioelectromagnetics

    pub_type: 临床试验,杂志文章

    doi:10.1002/bem.21695

    authors: Kyriakou A,Christ A,Neufeld E,Kuster N

    更新日期:2012-07-01 00:00:00

  • The effects of 884 MHz GSM wireless communication signals on headache and other symptoms: an experimental provocation study.

    abstract::Findings from prior studies of possible health and physiological effects from mobile phone use have been inconsistent. Exposure periods in provocation studies have been rather short and personal characteristics of the participants poorly defined. We studied the effect of radiofrequency field (RF) on self-reported symp...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20379

    authors: Hillert L,Akerstedt T,Lowden A,Wiholm C,Kuster N,Ebert S,Boutry C,Moffat SD,Berg M,Arnetz BB

    更新日期:2008-04-01 00:00:00

  • Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field-free conditions.

    abstract::Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised usi...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:

    authors: Tipping DR,Chapman KE,Birley AJ,Anderson M

    更新日期:1999-01-01 00:00:00

  • Pulsed electromagnetic fields stimulation affects BMD and local factor production of rats with disuse osteoporosis.

    abstract::Pulsed electromagnetic fields (PEMF) have been used widely to treat nonunion fractures and related problems in bone healing, as a biological and physical method. With the use of Helmholtz coils and PEMF stimulators to generate uniform time-varying electromagnetic fields, the effects of extremely low frequency electrom...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20535

    authors: Shen WW,Zhao JH

    更新日期:2010-02-01 00:00:00

  • Current density threshold for the stimulation of neurons in the motor cortex area.

    abstract::The aim of this study was to determine a current density threshold for exciting the motor cortex area of the brain. The current density threshold for excitation of nerve fibres (20 microm in diameter) found in the literature is approximately 1 A/m(2) at frequencies lower than 1 kHz. In consideration of a safety factor...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.10036

    authors: Kowalski T,Silny J,Buchner H

    更新日期:2002-09-01 00:00:00

  • The remote sensing of mental stress from the electromagnetic reflection coefficient of human skin in the sub-THz range.

    abstract::Recent work has demonstrated that the reflection coefficient of human skin in the frequency range from 95 to 110 GHz (W band) mirrors the temporal relaxation of stress induced by physical exercise. In this work, we extend these findings to show that in the event of a subtle trigger to stress, such as mental activity, ...

    journal_title:Bioelectromagnetics

    pub_type: 临床试验,杂志文章

    doi:10.1002/bem.21698

    authors: Safrai E,Ishai PB,Caduff A,Puzenko A,Polsman A,Agranat AJ,Feldman Y

    更新日期:2012-07-01 00:00:00

  • Bacterial response to the exposure of 50 Hz electromagnetic fields.

    abstract::To investigate the ability of prokaryotic microorganisms to activate strategies in adapting themselves to the environmental stress induced by exposure to extremely low frequency electromagnetic fields (ELF-EMF), cultures of Escherichia coli ATCC 700926 exposed at 50 Hz EMF (0.1, 0.5, 1.0 mT), and the respective sham-e...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20391

    authors: Cellini L,Grande R,Di Campli E,Di Bartolomeo S,Di Giulio M,Robuffo I,Trubiani O,Mariggiò MA

    更新日期:2008-05-01 00:00:00

  • Synaptosome behaviour is unaffected by weak pulsed electromagnetic fields.

    abstract::The present study examined the effect on rat cortical synaptosomes of a 2 h exposure to 50-Hz electromagnetic fields (EMFs) with a peak magnetic field of 2 mT. We measured modifications of synaptosomal mitochondrial respiration rate, ATP production, membrane potential, intrasynaptosomal Ca(2+) concentration and free i...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20322

    authors: Aldinucci C,Carretta A,Maiorca S,Rossi V,Ciccoli L,Pessina GP

    更新日期:2007-09-01 00:00:00

  • Influence of extremely low frequency electromagnetic fields on the swimming behavior of ciliates.

    abstract::Different species of ciliates (Paramecium biaurelia, Loxodes striatus, Tetrahymena thermophila) have been taken as model systems to study the effects of extremely low-frequency electromagnetic fields (50 Hz, 0.5-2.0 mT) on the cellular level. A dose-dependent increase in the mean swimming velocity and a decrease in th...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(sici)1521-186x(1997)18:7<491::aid-bem4>3.

    authors: Hemmersbach R,Becker E,Stockem W

    更新日期:1997-01-01 00:00:00

  • Effects of 2.45-GHz microwaves on primate corneal endothelium.

    abstract::Both eyes of anesthetized cynomolgus monkeys (Macaca fascicularis) were irradiated with 2.45-GHz microwaves, either pulsed or continuous wave. In vivo corneal endothelial abnormalities were observed by specular microscopy and confirmed through histologic techniques after a 16- to 48-hour postexposure period. Pulsed mi...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250060209

    authors: Kues HA,Hirst LW,Lutty GA,D'Anna SA,Dunkelberger GR

    更新日期:1985-01-01 00:00:00

  • Frequency-dependent interference by magnetic fields of nerve growth factor-induced neurite outgrowth in PC-12 cells.

    abstract::We have shown that 50 Hz sinusoidal magnetic fields within the 5-10 microTesla (microT) rms range cause an intensity-dependent reduction in nerve growth factor (NGF) stimulation of neurite outgrowth (NO) in PC-12 cells. Here we report on the frequency dependence of this response over the 15-70 Hz range at 5 Hz interva...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250160607

    authors: Blackman CF,Benane SG,House DE

    更新日期:1995-01-01 00:00:00

  • Low-Frequency Magnetic Fields in Cars and Office Premises and the Geomagnetic Field Variations.

    abstract::In this study, we measured and analyzed the spectral characteristics of a low-frequency magnetic field (MF) inside several gasoline-powered cars while driving on busy city roads. The spectra obtained upon measurements in the interior of the cars are compared with those measured in office locations at different times o...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22269

    authors: Sarimov R,Binhi V

    更新日期:2020-07-01 00:00:00

  • Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans.

    abstract::Reports that low-intensity microwave radiation induces heat-shock reporter gene expression in the nematode, Caenorhabditis elegans, have recently been reinterpreted as a subtle thermal effect caused by slight heating. This study used a microwave exposure system (1.0 GHz, 0.5 W power input; SAR 0.9-3 mW kg(-1) for 6-we...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20515

    authors: Dawe AS,Bodhicharla RK,Graham NS,May ST,Reader T,Loader B,Gregory A,Swicord M,Bit-Babik G,de Pomerai DI

    更新日期:2009-12-01 00:00:00

  • Predicted SAR in Sprague-Dawley rat as a function of permittivity values.

    abstract::Specific absorption rate (SAR) value is dependent on permittivity value. However, variability in the published permittivity values for human and animal tissue and the development of sophisticated 3-dimensional digital anatomical models to predict SAR values has resulted in the need to understand how model parameters (...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.66

    authors: Gajsek P,Ziriax JM,Hurt WD,Walters TJ,Mason PA

    更新日期:2001-09-01 00:00:00

  • Inhibition of growth rate of Escherichia coli induced by extremely low-frequency weak magnetic fields.

    abstract::Cultures of Escherichia coli kept at 0 degree C in a phosphate buffer solution were exposed to a sinusoidal weak 60- or 600-Hz magnetic field of strength 2 X 10(-3) Tesla. A decrease of more than 40% in bacterial count was observed after a 60-h exposure to the magnetic field. Electron micrographs of exposed bacteria s...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250020310

    authors: Ramon C,Ayaz M,Streeter DD Jr

    更新日期:1981-01-01 00:00:00

  • Synthesis of diazoluminomelanin (DALM) in HL-60 cells for possible use as a cellular-level microwave dosimeter.

    abstract::Chemical and bacterial synthesis of a thermochemiluminescent polymer known as diazoluminomelanin (DALM) has been previously reported. This paper focuses on the intracellular synthesis of aminomelanin (AM) in mammalian cell lines and subsequent DALM synthesis from this core molecule. B16 melanoma cells, HL-60 myeloid l...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250150406

    authors: Bruno JG,Kiel JL

    更新日期:1994-01-01 00:00:00

  • Validity of surrogates for determination of 30-1000 Hz magnetic field exposure for video display terminal users in office settings.

    abstract::In studies investigating adverse reproductive outcomes associated with video display terminal (VDT) usage, amounts of time spent in front of a VDT or magnetic field (MF) levels in front of the VDT are used as surrogate measures of subject's MF exposure. However, the relevance of such surrogates to actual exposures has...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-186X(1996)17:5<406::AID-BEM8>3.

    authors: Abdollahzadeh S,Hammond SK,Schenker MB

    更新日期:1996-01-01 00:00:00

  • Do people understand IARC's 2B categorization of RF fields from cell phones?

    abstract::In May 2011, the International Agency on Cancer in Research (IARC) issued an official statement concluding that cell phone usage was "possibly carcinogenic to humans." There have been considerable doubts that non-experts and experts alike fully understood what IARC's categorization actually meant, as "possibly carcino...

    journal_title:Bioelectromagnetics

    pub_type: 信件

    doi:10.1002/bem.21851

    authors: Wiedemann PM,Boerner FU,Repacholi MH

    更新日期:2014-07-01 00:00:00

  • Effects of extremely-low-frequency magnetic fields on biological magnetite.

    abstract::Adair [Bioelectromagnetics 14:1-4, 1993] writes that "the effects of 60 Hz magnetic fields of 5 microT (50 mG) or less on biological structures holding magnetite (Fe3O4) are shown to be much smaller than those from thermal agitation; hence such interactions cannot be expected to be biologically significant." This conc...

    journal_title:Bioelectromagnetics

    pub_type: 评论,杂志文章

    doi:10.1002/bem.2250150308

    authors: Polk C

    更新日期:1994-01-01 00:00:00

  • Wire codes, magnetic fields, and childhood cancer.

    abstract::Childhood cancer has been modestly associated with wire codes, an exposure surrogate for power frequency magnetic fields, but less consistently with measured fields. We analyzed data on the population distribution of wire codes and their relationship with several measured magnetic field metrics. In a given geographic ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1521-186x(1997)18:2<99::aid-bem2>3.0

    authors: Kheifets LI,Kavet R,Sussman SS

    更新日期:1997-01-01 00:00:00

  • Exposure system to study hypotheses of ELF and RF electromagnetic field interactions of mobile phones with the central nervous system.

    abstract::A novel exposure system for double-blind human electromagnetic provocation studies has been developed that satisfies the precision, control of fields and potential artifacts, and provides the flexibility to investigate the response of hypotheses-driven electromagnetic field exposure schemes on brain function, ranging ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.21710

    authors: Murbach M,Christopoulou M,Crespo-Valero P,Achermann P,Kuster N

    更新日期:2012-09-01 00:00:00

  • Measurement of magnetically induced electric fields in conductive media near a 60 Hz current-carrying wire.

    abstract::Electric fields induced in a conductive body by the magnetic field of a current-carrying wire were analyzed theoretically and experimentally to assess the dosimetric importance of highly nonuniform, field-exposure conditions. Experimentation revealed that a 60-Hz magnetic field was inversely proportional to the radius...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250130305

    authors: Robertson-De Mers KA,Miller DL

    更新日期:1992-01-01 00:00:00

  • Investigations of a simulated geomagnetic field experienced by the International Space Station on attentional performance.

    abstract::We have previously reported that the exposure to an abnormal magnetic field simulating the one encountered by the International Space Station (ISS) orbiting around the Earth may enhance autonomic response to emotional stimuli. Here we report the results of the second part of that study which tested whether this field ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20438

    authors: Del Seppia C,Mezzasalma L,Messerotti M,Cordelli A,Ghione S

    更新日期:2009-01-01 00:00:00

  • Theoretical evaluation of magnetoreception of power-frequency fields.

    abstract::Several effects of power-frequency (50/60 Hz) magnetic fields (PF-MF) of weak intensity have been hypothesized in animals and humans. No valid mechanism, however, has been proposed for an interaction between PF-MF and biological tissues and living beings at intensities relevant to animal and human exposure. Here we pr...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20568

    authors: Vanderstraeten J,Gillis P

    更新日期:2010-07-01 00:00:00

  • Space charge drift from a +/- 400-kV direct current transmission line.

    abstract::The Minnesota Environmental Quality Board completed a 3-year study of the space charge plume outside the right of way of a bipolar (+/- 400 kV) direct current transmission line in rural west central Minnesota. The purpose of the study was to determine the levels of DC potential gradient and small airs ions in the down...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250070404

    authors: Hendrickson RC

    更新日期:1986-01-01 00:00:00