Abstract:
:Human glioma MO54 cells were used to investigate whether radio frequency (RF) field exposure could activate stress response genes. Cells were exposed to continuous wave 1950 MHz or sham conditions for up to 2 h. Specific absorption rates (SARs) were 1, 2, and 10 W/kg. For the cell growth experiment, cell numbers were counted at 0-4 days after exposure. Expression of Hsp27 and Hsp70, as well as the level of phosphorylated Hsp27 (78Ser) protein, was determined by Western blotting. It was found that sham exposed and RF exposed cells demonstrated a similar growth pattern up to 4 days after RF field exposure. RF field exposure at both 2 and 10 W/kg did not affect the growth of MO54 cells. In addition, there were no significant differences in protein expression of Hsp27 and Hsp70 between sham exposed and RF exposed cells at a SAR of 1, 2, or 10 W/kg for 1 and 2 h. However, exposure to RF field at a SAR of 10 W/kg for 1 and 2 h decreased the protein level of phosphorylated Hsp27 (78Ser) significantly. Our results suggest that although exposure to a 1950 MHz RF field has no effect on cell proliferation and expression of Hsp 27 and Hsp70, it may inhibit the phosphorylation of Hsp27 at Serine 78 in MO54 cells.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Miyakoshi J,Takemasa K,Takashima Y,Ding GR,Hirose H,Koyama Sdoi
10.1002/bem.20077subject
Has Abstractpub_date
2005-05-01 00:00:00pages
251-7issue
4eissn
0197-8462issn
1521-186Xjournal_volume
26pub_type
杂志文章abstract::In-vitro studies of biological effects of electromagnetic fields are often conducted with cultured cells either in suspension or grown in a monolayer. In the former case, the exposed medium can be assumed to be homogeneous; however, eventually the cells settle to the bottom of the container forming a two layer system ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140106
更新日期:1993-01-01 00:00:00
abstract::To evaluate duration- and dose-dependent effects of continuous exposure to a 60 Hz magnetic field (MF) on the testes in mice, BALB/c male mice were exposed to a 60 Hz MF at 100 μT for 24 h a day for 2, 4, 6, or 8 weeks, and at 2, 20, or 200 μT for 24 h a day for 8 weeks. Any exposures to MF did not significantly affec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21819
更新日期:2014-02-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20552
更新日期:2010-04-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章,多中心研究
doi:10.1002/bem.21817
更新日期:2014-01-01 00:00:00
abstract::The controversy over the possible association between magnetic field exposure and childhood leukemia has led several researchers to summarize the literature using meta-analysis. This paper reviews these previous meta-analyses and extends them by adding results from four studies published since the most recent analysis...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,meta分析
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更新日期:2001-01-01 00:00:00
abstract::The objective of this study was to investigate if persons with implantable medical devices are intrinsically protected by the current electromagnetic safety guidelines. For inter-laboratory comparisons, the U.S. Food and Drug Administration has defined a generic implant as consisting of an insulated wire with noninsul...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.21695
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abstract::Findings from prior studies of possible health and physiological effects from mobile phone use have been inconsistent. Exposure periods in provocation studies have been rather short and personal characteristics of the participants poorly defined. We studied the effect of radiofrequency field (RF) on self-reported symp...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20379
更新日期:2008-04-01 00:00:00
abstract::Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised usi...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Pulsed electromagnetic fields (PEMF) have been used widely to treat nonunion fractures and related problems in bone healing, as a biological and physical method. With the use of Helmholtz coils and PEMF stimulators to generate uniform time-varying electromagnetic fields, the effects of extremely low frequency electrom...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20535
更新日期:2010-02-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.10036
更新日期:2002-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.21698
更新日期:2012-07-01 00:00:00
abstract::To investigate the ability of prokaryotic microorganisms to activate strategies in adapting themselves to the environmental stress induced by exposure to extremely low frequency electromagnetic fields (ELF-EMF), cultures of Escherichia coli ATCC 700926 exposed at 50 Hz EMF (0.1, 0.5, 1.0 mT), and the respective sham-e...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20391
更新日期:2008-05-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20322
更新日期:2007-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:7<491::aid-bem4>3.
更新日期:1997-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250060209
更新日期:1985-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160607
更新日期:1995-01-01 00:00:00
abstract::In this study, we measured and analyzed the spectral characteristics of a low-frequency magnetic field (MF) inside several gasoline-powered cars while driving on busy city roads. The spectra obtained upon measurements in the interior of the cars are compared with those measured in office locations at different times o...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22269
更新日期:2020-07-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20515
更新日期:2009-12-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.66
更新日期:2001-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 信件
doi:10.1002/bem.21851
更新日期:2014-07-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 评论,杂志文章
doi:10.1002/bem.2250150308
更新日期:1994-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章,评审
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更新日期:1997-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21710
更新日期:2012-09-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130305
更新日期:1992-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20438
更新日期:2009-01-01 00:00:00
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journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20568
更新日期:2010-07-01 00:00:00
abstract::The Minnesota Environmental Quality Board completed a 3-year study of the space charge plume outside the right of way of a bipolar (+/- 400 kV) direct current transmission line in rural west central Minnesota. The purpose of the study was to determine the levels of DC potential gradient and small airs ions in the down...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250070404
更新日期:1986-01-01 00:00:00