Synthesis of diazoluminomelanin (DALM) in HL-60 cells for possible use as a cellular-level microwave dosimeter.

Abstract:

:Chemical and bacterial synthesis of a thermochemiluminescent polymer known as diazoluminomelanin (DALM) has been previously reported. This paper focuses on the intracellular synthesis of aminomelanin (AM) in mammalian cell lines and subsequent DALM synthesis from this core molecule. B16 melanoma cells, HL-60 myeloid leukemia cells, and RAW 264.7 macrophages show AM and DALM production. Macroscopic image analysis of HL-60 cell lysates containing DALM using the Quantitative Luminescence Imaging System (QLIS) showed increased chemiluminescence (CL) with increased microwave power input and increased temperature. This work represents a first step toward the goal of microscopic radiofrequency dosimetry of individual DALM-loaded cells using image analysis.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Bruno JG,Kiel JL

doi

10.1002/bem.2250150406

subject

Has Abstract

pub_date

1994-01-01 00:00:00

pages

315-28

issue

4

eissn

0197-8462

issn

1521-186X

journal_volume

15

pub_type

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