The interphase interval within a bipolar nanosecond electric pulse modulates bipolar cancellation.

Abstract:

:Nanosecond electric pulse (nsEP) exposure generates an array of physiological effects. The extent of these effects is impacted by whether the nsEP is a unipolar (UP) or bipolar (BP) exposure. A 600 ns pulse can generate 71% more YO-PRO-1 uptake compared to a 600 ns + 600 ns pulse exposure. This observation is termed "bipolar cancellation" (BPC) because despite the BP nsEP consisting of an additional 600 ns pulse, it generates reduced membrane perturbation. BPC is achieved by varying pulse amplitudes, and symmetrical and asymmetric pulse widths. The effect appears to reverse by increasing the interphase interval between symmetric BP pulses, suggesting membrane recovery is a BPC factor. To date, the impact of the interphase interval between asymmetrical BP and other BPC-inducing symmetrical BP nsEPs has not been fully explored. Additionally, interpulse intervals beyond 50 μs have not been explored to understand the impact of time between the BP nsEP phases. Here, we surveyed different interphase intervals among symmetrical and asymmetrical BP nsEPs to monitor their impact on BPC of YO-PRO-1 uptake. We identified that a 10 microsecond (ms) interphase interval within a symmetrical 600 ns + 600 ns, and 900 ns + 900 ns pulse can resolve BPC. Furthermore, the interphase interval to resolve asymmetric BPC from a 300 ns + 900 ns pulse versus 600 ns pulse exposure is greater (<10 ms) compared to symmetrical BP nsEPs. From these findings, we extended on our conceptual model that BPC is balanced by localized charging and discharging events across the membrane. Bioelectromagnetics. 39:441-450, 2018. Published 2018. This article is a U.S. Government work and is in the public domain in the USA.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Valdez CM,Barnes R Jr,Roth CC,Moen E,Ibey B

doi

10.1002/bem.22134

subject

Has Abstract

pub_date

2018-09-01 00:00:00

pages

441-450

issue

6

eissn

0197-8462

issn

1521-186X

journal_volume

39

pub_type

杂志文章
  • Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle.

    abstract::Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca(2+)](c)) plays an important role. To better understand the biological conditions under which ELF M...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20248

    authors: McCreary CR,Dixon SJ,Fraher LJ,Carson JJ,Prato FS

    更新日期:2006-07-01 00:00:00

  • Pulsed electromagnetic fields induced femoral metaphyseal bone thickness changes in the rat.

    abstract::The effect of 1 Hz, 30 mT pulsed magnetic fields on young adult rat femoral metaphyseal bone thickness was assessed. Ten same litter, female Wistar rats were studied; five of them underwent 30 min magnetic stimulation sessions for 20 consecutive days. The anterior and posterior cortical, as well as trabecular bone tra...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20396

    authors: Márquez-Gamiño S,Sotelo F,Sosa M,Caudillo C,Holguín G,Ramos M,Mesa F,Bernal J,Córdova T

    更新日期:2008-07-01 00:00:00

  • Differential intensity-dependent effects of pulsed electromagnetic fields on RANKL-induced osteoclast formation, apoptosis, and bone resorbing ability in RAW264.7 cells.

    abstract::Pulsed electromagnetic fields (PEMF) have been proven to be effective for promoting bone mass and regulating bone turnover both experimentally and clinically. However, the exact mechanisms for the regulation of PEMF on osteoclastogenesis as well as optical exposure parameters of PEMF on inhibiting osteoclastic activit...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22070

    authors: Wang P,Liu J,Yang Y,Zhai M,Shao X,Yan Z,Zhang X,Wu Y,Cao L,Sui B,Luo E,Jing D

    更新日期:2017-12-01 00:00:00

  • ELF magnetic field exposure system with feedback-controlled disturbance rejection.

    abstract::Extremely low-frequency (ELF) magnetic field exposure systems are usually subject to field disturbances induced by external sources. Here, a method for designing a feedback control system for cancelling the effect of external ELF magnetic field disturbances on the magnetic field over the exposure area is presented. Th...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(sici)1521-186x(1997)18:4<299::aid-bem2>3.

    authors: Wang PK

    更新日期:1997-01-01 00:00:00

  • Chronic exposure to ELF magnetic fields during night sleep with electric sheet: effects on diurnal melatonin rhythms in men.

    abstract::The possible effects of repeated night-time exposure to an extremely low frequency magnetic field (ELF-MF) on melatonin were investigated in nine healthy male subjects aged 23-37 yr. The 16-week experiment consisted of 3 weeks of pre-exposure, 11 weeks of night-time exposure to MF generated from a nonheated electric s...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/1521-186x(200102)22:2<138::aid-bem1017>3.0

    authors: Hong SC,Kurokawa Y,Kabuto M,Ohtsuka R

    更新日期:2001-02-01 00:00:00

  • Influence of 50 Hz magnetic field on sex hormones and other fertility parameters of adult male rats.

    abstract::The effects of an extremely low frequency (ELF) magnetic field on the sex hormones and other fertility parameters of adult male Sprague-Dawley rats were investigated. Adult male rats were exposed to a 50 Hz sinusoidal magnetic field at approximately 25 microT (rms) for 18 consecutive weeks. There were no significant e...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20186

    authors: Al-Akhras MA,Darmani H,Elbetieha A

    更新日期:2006-02-01 00:00:00

  • The Radiofrequency Radiation Dosimetry Handbook: reminiscences.

    abstract::This paper traces the history of the development of the Radiofrequency Radiation Dosimetry Handbook and its subsequent impact on radio frequency radiation exposure standards. The author's recollections are used to illustrate the behind the scenes efforts of the individuals involved in this project. The development of ...

    journal_title:Bioelectromagnetics

    pub_type: 历史文章,杂志文章

    doi:10.1002/(sici)1521-186x(1999)20:4+<9::aid-bem3>3.3

    authors: Allen SJ

    更新日期:1999-01-01 00:00:00

  • Neurochemical effects of a 20 kHz magnetic field on the central nervous system in prenatally exposed mice.

    abstract::C57/B1 mice were exposed during pregnancy (gestation days 0-19) to a 20 kHz magnetic field (MF). The asymmetric sawtooth-waveform magnetic field in the exposed racks had a flux density of 15 microT (peak to peak). After 19 days, the exposure was terminated, and the mice were housed individually under normal laboratory...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250160409

    authors: Dimberg Y

    更新日期:1995-01-01 00:00:00

  • Effects of a 50 Hz sinusoidal magnetic field on cell adhesion molecule expression in two human osteosarcoma cell lines (MG-63 and Saos-2).

    abstract::The possibility that a sinusoidal 50 Hz magnetic field with a magnetic flux density of 0.5 mT can induce variations in the expression of cell adhesion molecules (CAMs) in two human osteosarcoma cell lines (MG-63 and Saos-2) was investigated. In particular, the expression of two important integrins, VLA-2, the receptor...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.10113

    authors: Santini MT,Rainaldi G,Ferrante A,Indovina PL,Vecchia P,Donelli G

    更新日期:2003-07-01 00:00:00

  • Time trends (1998-2007) in brain cancer incidence rates in relation to mobile phone use in England.

    abstract::Mobile phone use in the United Kingdom and other countries has risen steeply since the early 1990's when the first digital mobile phones were introduced. There is an ongoing controversy about whether radio frequency (RF) exposure from mobile phones increases the risk of brain cancer. However, given the widespread use ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20648

    authors: de Vocht F,Burstyn I,Cherrie JW

    更新日期:2011-07-01 00:00:00

  • The biological impact of concurrent exposure to metallic nanoparticles and a static magnetic field.

    abstract::The rapid advancement of technology has led to an exponential increase of both nanomaterial and magnetic field utilization in applications spanning a variety of sectors. While extensive work has focused on the impact of these two variables on biological systems independently, the existence of any synergistic effects f...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.21790

    authors: Comfort KK,Maurer EI,Hussain SM

    更新日期:2013-10-01 00:00:00

  • The effect of pulsed and sinusoidal magnetic fields on the morphology of developing chick embryos.

    abstract::Several investigators have reported robust, statistically significant results that indicate that weak (approximately 1 microT) magnetic fields (MFs) increase the rate of morphological abnormalities in chick embryos. However, other investigators have reported that weak MFs do not appear to affect embryo morphology at a...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(sici)1521-186x(1997)18:6<431::aid-bem5>3.

    authors: Farrell JM,Litovitz TL,Penafiel M,Montrose CJ,Doinov P,Barber M,Brown KM,Litovitz TA

    更新日期:1997-01-01 00:00:00

  • Effects of static magnetic field on specific adenosine-5'- triphosphatase activities and bioelectrical and biomechanical properties in the rat diaphragm muscle.

    abstract::In this study, we aimed to clarify the effects of chronically applied static magnetic field (200 Gauss) on specific ATPase activities and bioelectrical and biomechanical responses in isolated rat diaphragm muscle. The mean activities of Na(+)-K+ ATPase and Ca2+ ATPase determined from the diaphragm homogenates were sig...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250160302

    authors: Iteğin M,Günay I,Loğoğlu G,Isbir T

    更新日期:1995-01-01 00:00:00

  • A biofilm growth protocol and the design of a magnetic field exposure setup to be used in the study of magnetic fields as a means of controlling bacterial biofilms.

    abstract::The use of prosthetic implants is increasing both in the United States and around the world and there is a concomitant rise in cases of biofilm-based, persistent infections that are quite serious and virtually impervious to antibiotic treatment. The development of alternate therapies that do not involve long term use ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20529

    authors: McLeod BR,Sandvik EL

    更新日期:2010-01-01 00:00:00

  • Coronary heart diseases: assessment of risk associated with work exposure to ultralow-frequency magnetic fields.

    abstract::The present analysis was stimulated by previous findings on the possible influence of natural ultralow-frequency (ULF; 0.001-10 Hz) geomagnetic field variations on the cardiovascular system and indications of an effect of man-made ULF magnetic fields on the rate of myocardial infarction. In the present study, we consi...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(SICI)1521-186X(1996)17:6<436::AID-BEM2>3.

    authors: Ptitsyna NG,Villoresi G,Kopytenko YA,Kudrin VA,Tyasto MI,Kopytenko EA,Iucci N,Voronov PM,Zaitsev DB

    更新日期:1996-01-01 00:00:00

  • Estimation of relative exposure levels for cellular phone users using a neural network.

    abstract::The wide and growing use of cellular phones has raised questions about the possible health risks associated with radio frequency (RF) electromagnetic fields. It would be helpful for epidemiologists as well as cellular phone users to obtain the relative exposure levels, because the RF exposure level is very difficult t...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20203

    authors: Kim SC,Nam KC,Kim DW

    更新日期:2006-09-01 00:00:00

  • Electric field exposure alters serum melatonin but not pineal melatonin synthesis in male rats.

    abstract::Sprague-Dawley male rats, maintained in a 14:10 h light:dark cycle were exposed for 30 days (starting at 56 days of age) to a 65 kV/m, 60 Hz electric field or to a sham field for 20 h/day beginning at dark onset. Pineal N-acetyltransferase (NAT), hydroxy-indole-o-methyl transferase (HIOMT), and melatonin as well as se...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250150506

    authors: Grota LJ,Reiter RJ,Keng P,Michaelson S

    更新日期:1994-01-01 00:00:00

  • Reactions of keratinocytes to in vitro millimeter wave exposure.

    abstract::The effects of millimeter waves (MW) on human keratinocytes were studied in vitro using the HaCaT keratinocyte cell line. MW-induced modulation of keratinocyte function was studied in proliferation, adhesion, chemotaxis, and interleukin-1beta (IL-1beta) production assays. Spontaneous proliferation, adhesion to tissue ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.62

    authors: Szabo I,Rojavin MA,Rogers TJ,Ziskin MC

    更新日期:2001-07-01 00:00:00

  • The effect of 20-week continuous 60 Hz magnetic field exposure on testicular function in sprague-dawley rats.

    abstract::Accumulating evidence does not yet confirm the effect of power line frequency magnetic field (MF) on human health and fertility. We recently reported that, at continuous 60 Hz MF exposure in mice, the dose given as magnetic flux density (tesla; T) and duration of exposure was related to induce testicular germ cell apo...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22146

    authors: Park S,Kim YJ,Kim MS,Kim HS,Kim MW,Kang YM,Lee SK,Choi KC,Kim N,Gimm YM,Kim YW

    更新日期:2018-10-01 00:00:00

  • A model for radiofrequency electromagnetic field predictions at outdoor and indoor locations in the context of epidemiological research.

    abstract::We present a geospatial model to predict the radiofrequency electromagnetic field from fixed site transmitters for use in epidemiological exposure assessment. The proposed model extends an existing model toward the prediction of indoor exposure, that is, at the homes of potential study participants. The model is based...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20552

    authors: Bürgi A,Frei P,Theis G,Mohler E,Braun-Fahrländer C,Fröhlich J,Neubauer G,Egger M,Röösli M

    更新日期:2010-04-01 00:00:00

  • Continuous exposure to 60 Hz magnetic fields induces duration- and dose-dependent apoptosis of testicular germ cells.

    abstract::To evaluate duration- and dose-dependent effects of continuous exposure to a 60 Hz magnetic field (MF) on the testes in mice, BALB/c male mice were exposed to a 60 Hz MF at 100 μT for 24 h a day for 2, 4, 6, or 8 weeks, and at 2, 20, or 200 μT for 24 h a day for 8 weeks. Any exposures to MF did not significantly affec...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.21819

    authors: Kim HS,Park BJ,Jang HJ,Ipper NS,Kim SH,Kim YJ,Jeon SH,Lee KS,Lee SK,Kim N,Ju YJ,Gimm YM,Kim YW

    更新日期:2014-02-01 00:00:00

  • Radiation from mobile phone systems: Is it perceived as a threat to people's health?

    abstract::The aim of this study was to explore the prevalence, nature and determinants of concerns about mobile phone radiation. We used data from a 2006 telephone survey of 1004 people aged 15+ years in Denmark. Twenty-eight percent of the respondents were concerned about exposure to mobile phone radiation; radiation from mast...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20484

    authors: Kristiansen IS,Elstein AS,Gyrd-Hansen D,Kildemoes HW,Nielsen JB

    更新日期:2009-07-01 00:00:00

  • Thermophysiological responses of human volunteers to whole body RF exposure at 220 MHz.

    abstract::Since 1994, our research has demonstrated how thermophysiological responses are mobilized in human volunteers exposed to three radio frequencies, 100, 450, and 2450 MHz. A significant gap in this frequency range is now filled by the present study, conducted at 220 MHz. Thermoregulatory responses of heat loss and heat ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20105

    authors: Adair ER,Blick DW,Allen SJ,Mylacraine KS,Ziriax JM,Scholl DM

    更新日期:2005-09-01 00:00:00

  • Low frequency amplitude modulated microwave fields change calcium efflux rates from synaptosomes.

    abstract::Calcium (45Ca2+) efflux from preloaded synaptosomes was studied with a continuous perfusion technique and the rate constants of a two-phase efflux process calculated. When 16-Hz sinusoidally amplitude modulated 450-MHz microwave field (maximal incident intensity 0.5 mW/cm2, modulation depth 75%) was applied during the...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250030303

    authors: Lin-Liu S,Adey WR

    更新日期:1982-01-01 00:00:00

  • Frequency-dependent interference by magnetic fields of nerve growth factor-induced neurite outgrowth in PC-12 cells.

    abstract::We have shown that 50 Hz sinusoidal magnetic fields within the 5-10 microTesla (microT) rms range cause an intensity-dependent reduction in nerve growth factor (NGF) stimulation of neurite outgrowth (NO) in PC-12 cells. Here we report on the frequency dependence of this response over the 15-70 Hz range at 5 Hz interva...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250160607

    authors: Blackman CF,Benane SG,House DE

    更新日期:1995-01-01 00:00:00

  • Lack of effect of 10 kV/m 60 Hz electric field exposure on pregnant dairy heifer hormones.

    abstract::Sixteen pregnant Holstein heifers weighing 521 +/- 46 kg, at 3.3 +/- 0.7 months of gestation and 2.2 +/- 2.0 months of age were confined to wooden metabolism cages and were exposed to a vertical electric field (EF) of 10.0 +/- 0.4 kV/m and an artificial light cycle of 12 h light-12 h dark. The heifers were divided int...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20020

    authors: Burchard JF,Nguyen DH,Monardes HG,Petitclerc D

    更新日期:2004-05-01 00:00:00

  • Interactive effects in 60-Hz electric-field exposure systems.

    abstract::The level of exposure of laboratory animals to 60-Hz electric fields is commonly specified in terms of the unperturbed field strength present before the introduction of experimental subjects and their cages. In the research reported in this paper, rats were housed in two parallel rows in 12.4 cm x 25.1 cm x 10.2 cm hi...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250020105

    authors: Kaune WT

    更新日期:1981-01-01 00:00:00

  • Radiofrequency radiation does not induce stress response in human T-lymphocytes and rat primary astrocytes.

    abstract::Heat shock proteins (HSPs) are rapidly induced by a variety of stressors, including heat shock, ethanol, heavy metals, UV, and gamma-radiation. Mitogen-activated protein kinases (MAPKs) are also involved in the stress transduction pathways in all eukaryotes. In this study, we attempted to determine whether radiofreque...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20235

    authors: Lee JS,Huang TQ,Kim TH,Kim JY,Kim HJ,Pack JK,Seo JS

    更新日期:2006-10-01 00:00:00

  • A circular waveguide irradiation system for nonhuman primates: design and dosimetry.

    abstract::A 275-MHz exposure system, consisting of a circular waveguide irradiator and a transparent plastic animal cage, has been developed to accommodate rhesus monkeys weighing up to 15 kg. The vertically oriented waveguide is composed primarily of stainless steel and is fitted with an inner cage fabricated from a tubular se...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250050109

    authors: Olsen RG,de Lorge JO,Forstall JR,Ezell CS

    更新日期:1984-01-01 00:00:00

  • Measurement of magnetically induced electric fields in conductive media near a 60 Hz current-carrying wire.

    abstract::Electric fields induced in a conductive body by the magnetic field of a current-carrying wire were analyzed theoretically and experimentally to assess the dosimetric importance of highly nonuniform, field-exposure conditions. Experimentation revealed that a 60-Hz magnetic field was inversely proportional to the radius...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250130305

    authors: Robertson-De Mers KA,Miller DL

    更新日期:1992-01-01 00:00:00