A biofilm growth protocol and the design of a magnetic field exposure setup to be used in the study of magnetic fields as a means of controlling bacterial biofilms.

Abstract:

:The use of prosthetic implants is increasing both in the United States and around the world and there is a concomitant rise in cases of biofilm-based, persistent infections that are quite serious and virtually impervious to antibiotic treatment. The development of alternate therapies that do not involve long term use of high levels of antibiotics or surgical intervention is needed. Based on the success of using electric or magnetic fields to alter certain physiological processes, it is hypothesized that relatively low level magnetic fields, in conjunction with the appropriate antibiotic, may be able to help control and eventually clear bacterial biofilms on a prosthetic. In order to test this hypothesis, it is necessary to first develop a means of growing laboratory grade biofilms on specific materials in a way that is repeatable between experiments and that can be reproduced by other laboratories. Secondly, a means of applying controlled magnetic fields to the surfaces supporting the biofilms at a defined temperature must be developed. This article addresses both of these points.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

McLeod BR,Sandvik EL

doi

10.1002/bem.20529

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

56-63

issue

1

eissn

0197-8462

issn

1521-186X

journal_volume

31

pub_type

杂志文章
  • Gibberellins are involved in effect of near-null magnetic field on Arabidopsis flowering.

    abstract::We previously found that flowering of Arabidopsis was suppressed by near-null magnetic field, which was related to the modification of cryptochrome. To disclose the physiological mechanism of this effect, we detected gibberellin (GA) levels and expressions of GA biosynthetic and signaling genes in wild type Arabidopsi...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22004

    authors: Xu C,Yu Y,Zhang Y,Li Y,Wei S

    更新日期:2017-01-01 00:00:00

  • The interphase interval within a bipolar nanosecond electric pulse modulates bipolar cancellation.

    abstract::Nanosecond electric pulse (nsEP) exposure generates an array of physiological effects. The extent of these effects is impacted by whether the nsEP is a unipolar (UP) or bipolar (BP) exposure. A 600 ns pulse can generate 71% more YO-PRO-1 uptake compared to a 600 ns + 600 ns pulse exposure. This observation is termed "...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22134

    authors: Valdez CM,Barnes R Jr,Roth CC,Moen E,Ibey B

    更新日期:2018-09-01 00:00:00

  • Neuronal outgrowth of PC-12 cells after combined treatment with nerve growth factor and a magnetic field: influence of the induced electric field strength.

    abstract::In view of possible therapeutic applications of magnetic fields, the effect of an enhancement of neuronal outgrowth at higher figures of flux density and induced field strength was investigated. On the average sinusoidal magnetic field treatment at 100 microTrms/50 Hz did not change nerve growth factor (NGF) induced n...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20073

    authors: Schimmelpfeng J,Weibezahn KF,Dertinger H

    更新日期:2005-01-01 00:00:00

  • Specific absorption rate in rats exposed to 2,450-MHz microwaves under seven exposure conditions.

    abstract::Both positive and negative biological effects of microwaves on drug actions in rats exposed to 1-mW/cm2, 2,450-MHz microwaves have been reported by several investigators. We conducted dosimetry studies for seven different exposure conditions to determine whether these different results could be due to the rats having ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250060108

    authors: Chou CK,Guy AW,McDougall JA,Lai H

    更新日期:1985-01-01 00:00:00

  • A possible involvement of beta-endorphin, substance P, and serotonin in rat analgesia induced by extremely low frequency magnetic field.

    abstract::Most of the research concerning magnetic antinociception was focused on brief exposure less than 1 h. The main purpose of the present study was to determine the effect of extremely low frequency (ELF) magnetic field (MF) repeated exposures on rats in inducing antinociception and to find the effective analgesic "time w...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20232

    authors: Bao X,Shi Y,Huo X,Song T

    更新日期:2006-09-01 00:00:00

  • Effects of exposure to a circularly polarized 50-Hz magnetic field on plasma and pineal melatonin levels in rats.

    abstract::We sought to determine whether a 6-week exposure to a 50-Hz rotating magnetic field influences melatonin synthesis by 11-18 week-old Wistar-King male rats. Rats were exposed continuously to a rotating magnetic field at 1, 5, 50, or 250 microT (spatial vector rms) for 6 weeks, except for twice-weekly breaks of about 2 ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250140203

    authors: Kato M,Honma K,Shigemitsu T,Shiga Y

    更新日期:1993-01-01 00:00:00

  • Calorimetric measurements of the effect of 330-MHz radiofrequency radiation on human erythrocyte ghosts.

    abstract::Irreversible changes in the heat capacity of human erythrocyte ghost suspensions due to the effect of 330-MHz radiofrequency radiation (at a specific absorption rate of approximately 9 mW/g) were detected by the method of scanning differential microcalorimetry. Using the data obtained from the analysis of infrared spe...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250050406

    authors: Shnyrov VL,Zhadan GG,Akoev IG

    更新日期:1984-01-01 00:00:00

  • The effect of 20-week continuous 60 Hz magnetic field exposure on testicular function in sprague-dawley rats.

    abstract::Accumulating evidence does not yet confirm the effect of power line frequency magnetic field (MF) on human health and fertility. We recently reported that, at continuous 60 Hz MF exposure in mice, the dose given as magnetic flux density (tesla; T) and duration of exposure was related to induce testicular germ cell apo...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22146

    authors: Park S,Kim YJ,Kim MS,Kim HS,Kim MW,Kang YM,Lee SK,Choi KC,Kim N,Gimm YM,Kim YW

    更新日期:2018-10-01 00:00:00

  • Intracellular calcium activity in isolated bovine adrenal chromaffin cells in the presence and absence of 60 Hz magnetic fields.

    abstract::This study examined whether 60 Hz magnetic field (MF) exposure alters intracellular calcium levels ([Ca(2+)](i)) in isolated bovine adrenal chromaffin cells, a classic model of neural responses. [Ca(2+)](i) was monitored by fluorescence video imaging of cells loaded with the calcium indicator fluo-4 during exposures t...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.10045

    authors: Craviso GL,Poss J,Lanctot C,Lundback SS,Chatterjee I,Publicover NG

    更新日期:2002-12-01 00:00:00

  • Effect of radiofrequency electromagnetic field exposure on in vitro models of neurodegenerative disease.

    abstract::In this work we tested viability, proliferation, and vulnerability of neural cells, after continuous radiofrequency (RF) electromagnetic fields exposure (global system for mobile telecommunications (GSM) modulated 900 MHz signal at a specific absorption rate (SAR) of 1 W/kg and maximum duration 144 h) generated by tra...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20507

    authors: Del Vecchio G,Giuliani A,Fernandez M,Mesirca P,Bersani F,Pinto R,Ardoino L,Lovisolo GA,Giardino L,Calzà L

    更新日期:2009-10-01 00:00:00

  • Magnetic field exposure assessment for adult residents of Maine who live near and far away from overhead transmission lines.

    abstract::Sixty-Hz magnetic field exposures were measured for 45 adult residents of Maine. Thirty of the subjects resided near rights-of-way (ROWs) with either 345- and 115-kV transmission lines, or ROWs with only 115-kV transmission lines; fifteen resided far from any transmission lines. Personal exposure data for a single 24-...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250130106

    authors: Kavet R,Silva JM,Thornton D

    更新日期:1992-01-01 00:00:00

  • Amyotrophic lateral sclerosis and occupational exposure to electromagnetic fields.

    abstract::In an hypothesis-generating case-control study of amyotrophic lateral sclerosis, lifetime occupational histories were obtained. The patients (n = 28) were clinic based. The occupational exposure of interest in this report is electromagnetic fields (EMFs). This is the first and so far the only exposure analyzed in this...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(sici)1521-186x(1997)18:1<28::aid-bem6>3.0

    authors: Davanipour Z,Sobel E,Bowman JD,Qian Z,Will AD

    更新日期:1997-01-01 00:00:00

  • Synaptosome behaviour is unaffected by weak pulsed electromagnetic fields.

    abstract::The present study examined the effect on rat cortical synaptosomes of a 2 h exposure to 50-Hz electromagnetic fields (EMFs) with a peak magnetic field of 2 mT. We measured modifications of synaptosomal mitochondrial respiration rate, ATP production, membrane potential, intrasynaptosomal Ca(2+) concentration and free i...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20322

    authors: Aldinucci C,Carretta A,Maiorca S,Rossi V,Ciccoli L,Pessina GP

    更新日期:2007-09-01 00:00:00

  • Lack of effects of 1439 MHz electromagnetic near field exposure on the blood-brain barrier in immature and young rats.

    abstract::Possible effects of 1439 MHz electromagnetic near field (EMF) exposure on the blood-brain barrier (BBB) were investigated using immature (4 weeks old) and young (10 weeks old) rats, equivalent in age to the time when the BBB development is completed and the young adult, respectively. Alteration of BBB related genes, s...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20138

    authors: Kuribayashi M,Wang J,Fujiwara O,Doi Y,Nabae K,Tamano S,Ogiso T,Asamoto M,Shirai T

    更新日期:2005-10-01 00:00:00

  • Perception of the electromagnetic field emitted by a mobile phone.

    abstract::Electromagnetic sensibility refers to the ability to perceive the electromagnetic field (EMF) without necessarily developing health symptoms attributed to EMF exposure. A large sample of young healthy adults (n = 84) performed two forced-choice tasks on the perception of the GSM mobile phone EMF (902 MHz pulsed at 217...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20375

    authors: Kwon MS,Koivisto M,Laine M,Hämäläinen H

    更新日期:2008-02-01 00:00:00

  • Resonant ac-dc magnetic fields: calculated response.

    abstract::An elementary model consisting of one charged particle in a viscous medium exposed to weak ac-dc low-frequency magnetic fields is analyzed to identify and explain the fundamental characteristics of the physical mechanisms that result in a resonance response, which is similar to the familiar cyclotron resonance. The mo...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250090402

    authors: Durney CH,Rushforth CK,Anderson AA

    更新日期:1988-01-01 00:00:00

  • Correlation between pulsed electromagnetic fields exposure time and cell proliferation increase in human osteosarcoma cell lines and human normal osteoblast cells in vitro.

    abstract::We have exposed cultured bone cells to a pulsed electromagnetic field (PEMF) for different times to find the minimal exposure time necessary to stimulate an increase of DNA synthesis. We used two different human osteosarcoma cell lines, TE-85 and MG-63, and human normal osteoblast cell (NHOC) obtained from surgical bo...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/(sici)1521-186x(1999)20:3<177::aid-bem4>3.

    authors: De Mattei M,Caruso A,Traina GC,Pezzetti F,Baroni T,Sollazzo V

    更新日期:1999-01-01 00:00:00

  • Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured tobacco cells.

    abstract::Effects of magnetic fields (MFs) on the activities of antioxidant enzymes of suspension-cultured tobacco cells were investigated. Compared with the control cells, exposure of the cells to static MF with the magnitudes of 10 and 30 mT for 5 days, 5 h each day, increased the activity of superoxide dismutase (SOD). In co...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20262

    authors: Sahebjamei H,Abdolmaleki P,Ghanati F

    更新日期:2007-01-01 00:00:00

  • No effects of pulsed radio frequency electromagnetic fields on melatonin, cortisol, and selected markers of the immune system in man.

    abstract::There is growing public concern that radio frequency electromagnetic fields may have adverse biological effects. In the present study eight healthy male students were tested to see whether or not radio frequency electromagnetic fields as used in modern digital wireless telecommunication (GSM standard) have noticeable ...

    journal_title:Bioelectromagnetics

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/bem.51

    authors: Radon K,Parera D,Rose DM,Jung D,Vollrath L

    更新日期:2001-05-01 00:00:00

  • Extremely low frequency electromagnetic field (ELF-EMF) reduces oxidative stress and improves functional and psychological status in ischemic stroke patients.

    abstract::As a result of ischaemia/reperfusion, massive generation of reactive oxygen species occurs, followed by decreased activity of antioxidant enzymes. Extremely low frequency electromagnetic fields (ELF-EMF) can modulate oxidative stress, but there are no clinical antioxidant studies in brain stroke patients. The aim of o...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.22055

    authors: Cichoń N,Bijak M,Miller E,Saluk J

    更新日期:2017-07-01 00:00:00

  • Dose dependence of acetylcholinesterase activity in neuroblastoma cells exposed to modulated radio-frequency electromagnetic radiation.

    abstract::Radio-frequency electromagnetic radiation (RFR) at 915 and 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. The dose-response relation is unusual, consisting of two power-density "windows" in which enhanced efflux occur...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250130407

    authors: Dutta SK,Das K,Ghosh B,Blackman CF

    更新日期:1992-01-01 00:00:00

  • Effects of millimeter waves on ionic currents of Lymnaea neurons.

    abstract::The effects of mm-waves 60.22-62.22 GHz and 75 GHz on A-type K+ currents and the effects of 61.22 GHz on Ca2+ currents of Lymnaea neurons were investigated using a whole-cell voltage-clamp technique. The open end of a rectangular waveguide covered with a thin Teflon film served as a radiator. Specific absorption rates...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:

    authors: Alekseev SI,Ziskin MC

    更新日期:1999-01-01 00:00:00

  • Simple nonperturbing temperature probe for microwave/radio frequency dosimetry.

    abstract::We present a simple readout device that fills the void produced by the gradual disappearance of the Vitck model 101 Electrothermia Monitor. The new device uses commercially available probes that are similar to Bowman's (IEEE Trans Microwave Theory Tech MTT-24:43-45, 1976) original design. As described, the device cove...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250100210

    authors: Olsen RG,Bowman RR

    更新日期:1989-01-01 00:00:00

  • Endogenous ionic currents and DC electric fields in multicellular animal tissues.

    abstract::Through the use of the non-invasive vibrating probe technique for detecting extracellular ionic currents developed in 1974 [Jaffe and Nuccitelli: J Cell Biol 63:614-628, 1974], embryonic currents have been detected in a wide range of animal systems (recently reviewed in [Nuccitelli, Noninvasive Techniques in Cell Biol...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,评审

    doi:10.1002/bem.2250130714

    authors: Nuccitelli R

    更新日期:1992-01-01 00:00:00

  • Numerical and analytical methods to determine the current density distributions produced in human and rat models by electric and magnetic fields.

    abstract::Some numerical and analytical methods used to estimate the internal electric fields and current densities produced within human and animal models by low-frequency electric and magnetic fields are surveyed. A major goal of such modeling is the design of laboratory experiments on cellular systems or animal models to pro...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,评审

    doi:10.1002/bem.2250130705

    authors: Hart FX

    更新日期:1992-01-01 00:00:00

  • Microwave dielectric studies on proteins, tissues, and heterogeneous suspensions.

    abstract::We summarize the results of several of our recent studies on the dielectric properties of protein solutions, tissues, and nonionic microemulsions at microwave frequencies extending to 18 GHz. The data in all cases are analyzed using the Maxwell mixture theory to determine the dielectric properties of the suspending wa...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250030108

    authors: Foster KR,Schepps JL,Epstein BR

    更新日期:1982-01-01 00:00:00

  • RF absorption involving biological macromolecules.

    abstract::The fundamental intramolecular frequency of a globular protein can be obtained from the measurements of acoustic velocities of bulk protein matter. This lowest frequency for common size molecules is shown to be above several hundred GHz. All modes below this frequency would then be intermolecular modes or bulk modes o...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.20013

    authors: Prohofsky EW

    更新日期:2004-09-01 00:00:00

  • Exposure to an inhomogeneous static magnetic field increases thermal pain threshold in healthy human volunteers.

    abstract::In the present experiment, the effect of a single 30 min inhomogeneous static magnetic field (SMF) exposure on thermal pain threshold (TPT) was examined in 15 young healthy human volunteers. The SMF had a maximum peak-to-peak amplitude of 330 mT with a maximum gradient of 13.2 T/m. In either of two experimental sessio...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/bem.20622

    authors: Kovács-Bálint Z,Csathó A,László JF,Juhász P,Hernádi I

    更新日期:2011-02-01 00:00:00

  • A computer model of temperature distribution inside a lossy sphere after microwave radiation.

    abstract::The temperature distribution inside a lossy sphere resulting from the absorption of microwave energy was approximated by successive numerical iterations. of the thermal energy equation. Heat transfer within the sphere by conduction was considered. In the model energy was not dissipated by convection but was contained ...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章

    doi:10.1002/bem.2250010309

    authors: Janna WS,Russo EP,McAfee R,Davoudi RM

    更新日期:1980-01-01 00:00:00

  • Occupational exposure levels of static magnetic field during routine MRI examination in 3T MR system.

    abstract::Occupational exposure to the high static magnetic fields (SMFs) during magnetic resonance imaging (MRI) examinations raises concerns of adverse health effects. In this study, personal exposure monitoring of the magnetic fields during routine examinations in two 3 T MRI systems was carried out. A three-axis Hall magnet...

    journal_title:Bioelectromagnetics

    pub_type: 杂志文章,多中心研究

    doi:10.1002/bem.21817

    authors: Yamaguchi-Sekino S,Nakai T,Imai S,Izawa S,Okuno T

    更新日期:2014-01-01 00:00:00