Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle.


:Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca(2+)](c)) plays an important role. To better understand the biological conditions under which ELF MF exposure might alter [Ca(2+)](c), we measured [Ca(2+)](c) by ratiometric fluorescence spectrophotometry during exposure to ELF MF in Jurkat E6.1 cells synchronized to different phases of the cell cycle. Suspensions of cells were exposed either to a near zero MF (Null) or a 60 Hz, 100 microT sinusoidal MF superimposed upon a collinear 78.1 microT static MF (AC + DC). An initial series of experiments indicated that the maximum increase in [Ca(2+)](c) above baseline after stimulation with anti-CD3 was significantly higher in samples exposed to AC + DC (n = 30) compared to Null (n = 30) with the largest difference in G2-M enriched samples. However, in a second study with G2-M enriched cells, samples treated with AC + DC (n = 17) were not statistically different from Null-treated samples (n = 27). Detailed analysis revealed that the dynamics in [Ca(2+)](c) before and after stimulation with anti-CD3 were dissimilar between Null samples from each study. From the results, we concluded (i) that the ELF MF increased [Ca(2+)](c) during an antibody-induced signaling event, (ii) that the ELF MF effect did not depend to a large degree on cell cycle, and (iii) that a field-related change in [Ca(2+)](c) signaling appeared to correlate with features in the [Ca(2+)](c) dynamics. Future work could evaluate [Ca(2+)](c) dynamics in relation to the phase of the cell cycle and inter-study variation, which may reveal factors important for the observation of real-time effects of ELF MF on [Ca(2+)](c).






McCreary CR,Dixon SJ,Fraher LJ,Carson JJ,Prato FS




Has Abstract


2006-07-01 00:00:00












  • Association of residential magnetic fields with contact voltage.

    abstract::The US National Electrical Code's (NEC) requirement to ground a home's electrical service to the residential water line results in a voltage between the water line and earth, V W-E. The voltage may result from ground return current that flows into the earth via the water line or from inductive effects from other sourc...


    pub_type: 杂志文章


    authors: Kavet R,Zaffanella LE,Pearson RL,Dallapiazza J

    更新日期:2004-10-01 00:00:00

  • A biofilm growth protocol and the design of a magnetic field exposure setup to be used in the study of magnetic fields as a means of controlling bacterial biofilms.

    abstract::The use of prosthetic implants is increasing both in the United States and around the world and there is a concomitant rise in cases of biofilm-based, persistent infections that are quite serious and virtually impervious to antibiotic treatment. The development of alternate therapies that do not involve long term use ...


    pub_type: 杂志文章


    authors: McLeod BR,Sandvik EL

    更新日期:2010-01-01 00:00:00

  • High-voltage overhead power lines in epidemiology: patterns of time variations in current load and magnetic fields.

    abstract::In epidemiological studies of electromagnetic fields and health effects, exposure classification is crucial. There is no generally accepted biophysical interaction mechanism, but many studies are based on the hypothesis of a causal relationship with the strength of magnetic field. Some definition of the magnitude of e...


    pub_type: 杂志文章


    authors: Reitan JB,Tynes T,Kvamshagen KA,Vistnes AI

    更新日期:1996-01-01 00:00:00

  • Exposure system to study hypotheses of ELF and RF electromagnetic field interactions of mobile phones with the central nervous system.

    abstract::A novel exposure system for double-blind human electromagnetic provocation studies has been developed that satisfies the precision, control of fields and potential artifacts, and provides the flexibility to investigate the response of hypotheses-driven electromagnetic field exposure schemes on brain function, ranging ...


    pub_type: 杂志文章


    authors: Murbach M,Christopoulou M,Crespo-Valero P,Achermann P,Kuster N

    更新日期:2012-09-01 00:00:00

  • Effects of 2.45-GHz microwaves on primate corneal endothelium.

    abstract::Both eyes of anesthetized cynomolgus monkeys (Macaca fascicularis) were irradiated with 2.45-GHz microwaves, either pulsed or continuous wave. In vivo corneal endothelial abnormalities were observed by specular microscopy and confirmed through histologic techniques after a 16- to 48-hour postexposure period. Pulsed mi...


    pub_type: 杂志文章


    authors: Kues HA,Hirst LW,Lutty GA,D'Anna SA,Dunkelberger GR

    更新日期:1985-01-01 00:00:00

  • Neuronal outgrowth of PC-12 cells after combined treatment with nerve growth factor and a magnetic field: influence of the induced electric field strength.

    abstract::In view of possible therapeutic applications of magnetic fields, the effect of an enhancement of neuronal outgrowth at higher figures of flux density and induced field strength was investigated. On the average sinusoidal magnetic field treatment at 100 microTrms/50 Hz did not change nerve growth factor (NGF) induced n...


    pub_type: 杂志文章


    authors: Schimmelpfeng J,Weibezahn KF,Dertinger H

    更新日期:2005-01-01 00:00:00

  • Effects of exposure to extremely low frequency magnetic fields on spermatogenesis in adult rats.

    abstract::The constant exposure of modern society to extremely low frequency magnetic fields (ELF-MF) has raised considerable concerns about the potential risks to male reproduction. However, the epidemiological and experimental data remain contradictory and inconclusive. In the present study, we investigated the effects of 50 ...


    pub_type: 杂志文章


    authors: Duan W,Liu C,Wu H,Chen C,Zhang T,Gao P,Luo X,Yu Z,Zhou Z

    更新日期:2014-01-01 00:00:00

  • Magnetite biomineralization and geomagnetic sensitivity in higher animals: an update and recommendations for future study.

    abstract::Magnetite, the only known biogenic material with ferromagnetic properties, has been identified as a biochemical precipitate in three of the five kingdoms of living organisms, with a fossil record that now extends back nearly 2 billion years. In the magnetotactic bacteria, protoctists, and fish, single-domain crystals ...


    pub_type: 杂志文章,评审


    authors: Kirschvink JL

    更新日期:1989-01-01 00:00:00

  • Extremely low frequency magnetic fields regulate differentiation of regulatory T cells: Potential role for ROS-mediated inhibition on AKT.

    abstract::Our previous studies showed that extremely low frequency magnetic fields (ELF-MFs) inhibited tumor growth and change proportion of splenic regulatory T cells (Treg cells). Here, we focus on the effect of ELF-MFs on lung metastatic melanoma mouse model and the regulatory mechanism of ELF-MFs on the differentiation of T...


    pub_type: 杂志文章


    authors: Tang R,Xu Y,Ma F,Ren J,Shen S,Du Y,Hou Y,Wang T

    更新日期:2016-02-01 00:00:00

  • Effects of 60-Hz electric fields on cellular elongation and radial expansion growth in cucurbit roots.

    abstract::Serial longitudinal and transverse sections were prepared from roots of Cucumis sativus and Cucurbita maxima that had been exposed/sham-exposed to 60-Hz electric fields for 0-2 days. Field exposures were selected to produce a 10-20% or a 70-80% growth inhibition in whole roots of both species. Cortical cell length and...


    pub_type: 杂志文章


    authors: Brayman AA,Miller MW,Cox C

    更新日期:1987-01-01 00:00:00

  • Predicted SAR in Sprague-Dawley rat as a function of permittivity values.

    abstract::Specific absorption rate (SAR) value is dependent on permittivity value. However, variability in the published permittivity values for human and animal tissue and the development of sophisticated 3-dimensional digital anatomical models to predict SAR values has resulted in the need to understand how model parameters (...


    pub_type: 杂志文章


    authors: Gajsek P,Ziriax JM,Hurt WD,Walters TJ,Mason PA

    更新日期:2001-09-01 00:00:00

  • Exposure to electromagnetic fields from smart utility meters in GB; part I) laboratory measurements.

    abstract::Laboratory measurements of electric fields have been carried out around examples of smart meter devices used in Great Britain. The aim was to quantify exposure of people to radiofrequency signals emitted from smart meter devices operating at 2.4 GHz, and then to compare this with international (ICNIRP) health-related ...


    pub_type: 杂志文章


    authors: Peyman A,Addison D,Mee T,Goiceanu C,Maslanyj M,Mann S

    更新日期:2017-05-01 00:00:00

  • Effects of 1.8 GHz radiofrequency field on microstructure and bone metabolism of femur in mice.

    abstract::To investigate the effects of 1.8 GHz radiofrequency (RF) field on bone microstructure and metabolism of femur in mice, C57BL/6 mice (male, age 4 weeks) were whole-body exposed or sham exposed to 1.8 GHz RF field. Specific absorption rates of whole body and bone were approximately 2.70 and 1.14 W/kg (6 h/day for 28 da...


    pub_type: 杂志文章


    authors: Guo L,Zhang JP,Zhang KY,Wang HB,Wang H,An GZ,Zhou Y,Meng GL,Ding GR

    更新日期:2018-07-01 00:00:00

  • Intracellular calcium activity in isolated bovine adrenal chromaffin cells in the presence and absence of 60 Hz magnetic fields.

    abstract::This study examined whether 60 Hz magnetic field (MF) exposure alters intracellular calcium levels ([Ca(2+)](i)) in isolated bovine adrenal chromaffin cells, a classic model of neural responses. [Ca(2+)](i) was monitored by fluorescence video imaging of cells loaded with the calcium indicator fluo-4 during exposures t...


    pub_type: 杂志文章


    authors: Craviso GL,Poss J,Lanctot C,Lundback SS,Chatterjee I,Publicover NG

    更新日期:2002-12-01 00:00:00

  • Lack of effect of 10 kV/m 60 Hz electric field exposure on pregnant dairy heifer hormones.

    abstract::Sixteen pregnant Holstein heifers weighing 521 +/- 46 kg, at 3.3 +/- 0.7 months of gestation and 2.2 +/- 2.0 months of age were confined to wooden metabolism cages and were exposed to a vertical electric field (EF) of 10.0 +/- 0.4 kV/m and an artificial light cycle of 12 h light-12 h dark. The heifers were divided int...


    pub_type: 杂志文章


    authors: Burchard JF,Nguyen DH,Monardes HG,Petitclerc D

    更新日期:2004-05-01 00:00:00

  • Low frequency magnetic field therapy in patients with cytostatic-induced polyneuropathy: a phase II pilot study.

    abstract::Cytostatic-induced polyneuropathy (CIPN) is a common and serious toxicity in tumor patients. Treatment and prophylactic measures are mainly ineffective. Therefore, there is an urgent need to establish a sufficient therapy for pPNP. Between July 2007 and August 2008, 20 patients were treated with low frequency (4-12 Hz...


    pub_type: 信件


    authors: Geiger G,Mikus E,Dertinger H,Rick O

    更新日期:2015-04-01 00:00:00

  • Morphological and electrophysiological changes produced by electrical stimulation in cultured neuroblastoma cells.

    abstract::Electric fields, which were equivalent to those generated by medical devices, were applied to cultured neuroblastoma cells (mouse and human) to test for morphological damage and to establish damage thresholds. Each of two methods of applying fields permitted flow of electrical current and minimized exposure of cells t...


    pub_type: 杂志文章


    authors: Krauthamer V,Bekken M,Horowitz JL

    更新日期:1991-01-01 00:00:00

  • Effect of microwave irradiation (2.45 GHz, CW) on egg weight loss, egg hatchability, and hatchling growth of the Coturnix quail.

    abstract::Fertile eggs of the Coturnix quail were exposed twice a day for 30 min to 2.45-GHz continuous wave radiation at power densities of 25 or 50 mW cm-2 throughout the 17-day incubation period. Other eggs were exposed to 20 degrees C or 24 degrees C temperatures twice daily. Repeated exposures to 20 degrees C, 24 degrees C...


    pub_type: 杂志文章


    authors: Byman D,Battista SP,Wasserman FE,Kunz TH

    更新日期:1985-01-01 00:00:00

  • Foot currents and ankle SARs induced by dielectric heaters.

    abstract::Data are presented on ankle-specific SARs and foot currents as a function of strengths of radio-frequency electromagnetic fields encountered by operators of dielectric heaters. The determination of foot currents was based on near-field exposures in which reactive coupling dominates, and which can result in substantial...


    pub_type: 杂志文章


    authors: Conover DL,Moss CE,Murray WE,Edwards RM,Cox C,Grajewski B,Werren DM,Smith JM

    更新日期:1992-01-01 00:00:00

  • Do people understand IARC's 2B categorization of RF fields from cell phones?

    abstract::In May 2011, the International Agency on Cancer in Research (IARC) issued an official statement concluding that cell phone usage was "possibly carcinogenic to humans." There have been considerable doubts that non-experts and experts alike fully understood what IARC's categorization actually meant, as "possibly carcino...


    pub_type: 信件


    authors: Wiedemann PM,Boerner FU,Repacholi MH

    更新日期:2014-07-01 00:00:00

  • Modeling the nuclear magnetic resonance behavior of lung: from electrical engineering to critical care medicine.

    abstract::The present article reviews the basic principles of a new approach to the characterization of pulmonary disease. This approach is based on the unique nuclear magnetic resonance (NMR) properties of the lung and combines experimental measurements (using specially developed NMR techniques) with theoretical simulations. T...


    pub_type: 杂志文章,评审


    authors: Cutillo AG,Ailion DC

    更新日期:1999-01-01 00:00:00

  • Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy.

    abstract::The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorb...


    pub_type: 杂志文章


    authors: Ikehara T,Yamaguchi H,Hosokawa K,Miyamoto H,Aizawa K

    更新日期:2003-10-01 00:00:00

  • Mobile phone ownership and use among school children in three Hungarian cities.

    abstract::In spite of rapid worldwide increase in mobile phone use and public concerns about associated potential health effects, little is known about patterns of mobile phone ownership and use in the general population and among children. In April 2005, we conducted a survey of mobile phone ownership and use among fourth grad...


    pub_type: 杂志文章


    authors: Mezei G,Benyi M,Muller A

    更新日期:2007-05-01 00:00:00

  • Dielectric properties of porcine brain tissue in the transition from life to death at frequencies from 800 to 1900 MHz.

    abstract::Ten experiments on pigs were performed to investigate possible postmortem changes of the dielectric properties of brain gray matter in the frequency range of 800-1900 MHz. After keeping the animals in stable anaesthesia for at least 45 min, they were euthanatised by an intravenous injection of hypertonic potassium chl...


    pub_type: 杂志文章


    authors: Schmid G,Neubauer G,Illievich UM,Alesch F

    更新日期:2003-09-01 00:00:00

  • Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured tobacco cells.

    abstract::Effects of magnetic fields (MFs) on the activities of antioxidant enzymes of suspension-cultured tobacco cells were investigated. Compared with the control cells, exposure of the cells to static MF with the magnitudes of 10 and 30 mT for 5 days, 5 h each day, increased the activity of superoxide dismutase (SOD). In co...


    pub_type: 杂志文章


    authors: Sahebjamei H,Abdolmaleki P,Ghanati F

    更新日期:2007-01-01 00:00:00

  • Response of perennial woody plants to seed treatment by electromagnetic field and low-temperature plasma.

    abstract::Radiofrequency (5.28 MHz) electromagnetic radiation and low-temperature plasma were applied as short-term (2-15 min) seed treatments to two perennial woody plant species, including Smirnov's rhododendron (Rhododendron smirnowii Trautv.) and black mulberry (Morus nigra L.). Potential effects were evaluated using germin...


    pub_type: 杂志文章


    authors: Mildaziene V,Pauzaite G,Malakauskiene A,Zukiene R,Nauciene Z,Filatova I,Azharonok V,Lyushkevich V

    更新日期:2016-12-01 00:00:00

  • Postmortem changes of the dielectric properties of bovine brain tissues at low radiofrequencies.

    abstract::The dielectric properties of the bovine brain grey and white matter in the frequency range from 20 kHz to 100 MHz were measured at different times following animal death. Changes in the dielectric parameters versus time are interpreted in terms of the reduction of the cell volume fraction that results from either cell...


    pub_type: 杂志文章


    authors: Surowiec A,Stuchly SS,Swarup A

    更新日期:1986-01-01 00:00:00

  • Numerical and analytical methods to determine the current density distributions produced in human and rat models by electric and magnetic fields.

    abstract::Some numerical and analytical methods used to estimate the internal electric fields and current densities produced within human and animal models by low-frequency electric and magnetic fields are surveyed. A major goal of such modeling is the design of laboratory experiments on cellular systems or animal models to pro...


    pub_type: 杂志文章,评审


    authors: Hart FX

    更新日期:1992-01-01 00:00:00

  • Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field-free conditions.

    abstract::Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised usi...


    pub_type: 杂志文章


    authors: Tipping DR,Chapman KE,Birley AJ,Anderson M

    更新日期:1999-01-01 00:00:00

  • Reproductive hazards among workers at high voltage substations.

    abstract::A retrospective study on reproductive hazards was performed among 542 employees at Swedish power plants. Questionnaires were answered by 89% of the employees. Data on pregnancies were checked by studying hospital case records. There was a statistically significant, decreased frequency of "normal" pregnancy outcome, al...


    pub_type: 杂志文章


    authors: Nordström S,Birke E,Gustavsson L

    更新日期:1983-01-01 00:00:00