A 0.5 G, 60 Hz magnetic field suppresses melatonin production in pinealocytes.

Abstract:

:The objective of this study was to develop a model for testing various hypotheses concerning possible mechanisms whereby electromagnetic fields might induce suppression of nighttime melatonin production in rodents. A published method for digesting freshly obtained pineal glands to the single cell level was modified, yielding better than 95% viability. An in vitro exposure facility developed for the Food and Drug Administration was used for 12-h overnight exposures of primary pinealocyte cultures to 0.05 mT, 60 Hz, vertical AC and 0.06 microT, DC fields. After exposure, cells were separated from the supernatant by centrifugation. Supernatant melatonin was measured by ELISA assays. Data from 10 experiments demonstrated an average 46% reduction in norepinephrine-induced production of melatonin in the pinealocytes. The results support the hypothesis that EM exposure can produce pineal gland melatonin suppression by affecting individual cells.

journal_name

Bioelectromagnetics

journal_title

Bioelectromagnetics

authors

Rosen LA,Barber I,Lyle DB

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

123-7

issue

2

eissn

0197-8462

issn

1521-186X

pii

10.1002/(SICI)1521-186X(1998)19:2<123::AID-BEM11>3

journal_volume

19

pub_type

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