Abstract:
:We investigated the effects on human keratinocytes (HaCaT) of exposure to a sinusoidal magnetic field of 2 mT (50 Hz). These cells are a good model for studying interaction of nonionising radiation, because they are not shielded from fields in vivo and also because they are resistant to both mechanical and thermal stimuli. We performed scanning microscopy which showed modification in shape and morphology in exposed cells. This modification is related to differential actin distribution as revealed by phalloidin fluorescence analysis. Moreover, the exposed cells show increased clonogenic capacity, as well as increased cellular growth as showed by clonogenicity assays and growth curves. Indirect immunofluorescence analysis using a fluorescent antibody against involucrin and beta4 integrin, which are respectively differentiation and adhesion markers, revealed an increase of involucrin expression and segregation of beta4 integrin in the cell membrane in cells exposed to 50 Hz; a higher percentage of the exposed cells shows a modified pattern of adhesion and differentiation markers. We also present evidence that exposure of HaCaT cells can interfere with protein kinase activity. Our observations confirm the hypothesis that electromagnetic fields at 50 Hz may modify cell membrane morphology and interfere with initiation of the signal cascade pathway and cellular adhesion.
journal_name
Bioelectromagneticsjournal_title
Bioelectromagneticsauthors
Manni V,Lisi A,Pozzi D,Rieti S,Serafino A,Giuliani L,Grimaldi Sdoi
10.1002/bem.10023subject
Has Abstractpub_date
2002-05-01 00:00:00pages
298-305issue
4eissn
0197-8462issn
1521-186Xpii
10.1002/bem.10023journal_volume
23pub_type
杂志文章abstract::The possible effects of repeated night-time exposure to an extremely low frequency magnetic field (ELF-MF) on melatonin were investigated in nine healthy male subjects aged 23-37 yr. The 16-week experiment consisted of 3 weeks of pre-exposure, 11 weeks of night-time exposure to MF generated from a nonheated electric s...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/1521-186x(200102)22:2<138::aid-bem1017>3.0
更新日期:2001-02-01 00:00:00
abstract::The present study investigates the effects of a weak (+/-200 microT(pk)), pulsed, extremely low frequency magnetic field (ELF MF) upon the human electroencephalogram (EEG). We have previously determined that exposure to pulsed ELF MFs can affect the EEG, notably the alpha frequency (8-13 Hz) over the occipital-parieta...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20434
更新日期:2009-01-01 00:00:00
abstract::Cytostatic-induced polyneuropathy (CIPN) is a common and serious toxicity in tumor patients. Treatment and prophylactic measures are mainly ineffective. Therefore, there is an urgent need to establish a sufficient therapy for pPNP. Between July 2007 and August 2008, 20 patients were treated with low frequency (4-12 Hz...
journal_title:Bioelectromagnetics
pub_type: 信件
doi:10.1002/bem.21897
更新日期:2015-04-01 00:00:00
abstract::We present a geospatial model to predict the radiofrequency electromagnetic field from fixed site transmitters for use in epidemiological exposure assessment. The proposed model extends an existing model toward the prediction of indoor exposure, that is, at the homes of potential study participants. The model is based...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20552
更新日期:2010-04-01 00:00:00
abstract::This paper traces the history of the development of the Radiofrequency Radiation Dosimetry Handbook and its subsequent impact on radio frequency radiation exposure standards. The author's recollections are used to illustrate the behind the scenes efforts of the individuals involved in this project. The development of ...
journal_title:Bioelectromagnetics
pub_type: 历史文章,杂志文章
doi:10.1002/(sici)1521-186x(1999)20:4+<9::aid-bem3>3.3
更新日期:1999-01-01 00:00:00
abstract::Despite experimental evidence supporting ICR-like interactions in biological systems, to date there is no reasonable theoretical explanation for this phenomenon. The parametric resonance approach introduced by Lednev has enjoyed limited success in predicting the response as a function of the ratio of AC magnetic inten...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20406
更新日期:2008-07-01 00:00:00
abstract::Low-energy pulsed electromagnetic field (PEMF) therapy has been suggested as a promising therapy to increase microcirculation, which is of great concern in patients with fibromyalgia. This study evaluated the effectiveness of PEMF therapy on the treatment of fibromyalgia. A group of 108 women with fibromyalgia were al...
journal_title:Bioelectromagnetics
pub_type: 杂志文章,随机对照试验
doi:10.1002/bem.22127
更新日期:2018-07-01 00:00:00
abstract::Previously, we have reported that exposure to 50 Hz coherent sinusoidal magnetic fields (MF) for 24 h inhibits gap junction intercellular communication (GJIC) in mammalian cells at an intensity of 0.4 mT and enhances the inhibition effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) at 0.2 mT. In the present study, w...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20207
更新日期:2006-05-01 00:00:00
abstract::Treatment with direct electric current (DC) can inhibit tumor growth in several systems. To evaluate the cellular reactions generated by this treatment, we stimulated mouse mastocytoma P815 cells with DC and examined their viability and ultrastructural characteristics, as well as the effect of DC on surface carbohydra...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/1521-186x(200012)21:8<597::aid-bem6>3.0.co
更新日期:2000-12-01 00:00:00
abstract::Cell-based or magnetic field therapies as alternative approaches to pain management have been tested in several experimental pain models. The aim of this study therefore was to investigate the actions of the cell-based therapy (adipose tissue derived mesenchymal stem cells; ADMSC) or pulsed magnetic field (PMF) therap...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.22038
更新日期:2017-05-01 00:00:00
abstract::The impedance spectroscopy technique (IST) was used for studying the effect of a 0.5 T magnetic field on the electrical properties of whole human blood. A Solartron SI 1260 spectrometer was used to measure the impedance spectra of magnetic field exposed blood samples compared to non-exposed samples. An equivalent elec...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20132
更新日期:2005-10-01 00:00:00
abstract::Irreversible changes in the heat capacity of human erythrocyte ghost suspensions due to the effect of 330-MHz radiofrequency radiation (at a specific absorption rate of approximately 9 mW/g) were detected by the method of scanning differential microcalorimetry. Using the data obtained from the analysis of infrared spe...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250050406
更新日期:1984-01-01 00:00:00
abstract::The effect of millimeter waves (MMWs) on the genome conformational state (GCS) of E. coli AB1157 cells was studied by the method of anomalous viscosity time dependencies (AVTD) in the frequency range of 51.64-51.85 GHz. The 51.755 GHz resonance frequency of the cell reaction to MMWs did not depend on power density (PD...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(SICI)1521-186X(1996)17:4<312::AID-BEM7>3.
更新日期:1996-01-01 00:00:00
abstract::Several effects of power-frequency (50/60 Hz) magnetic fields (PF-MF) of weak intensity have been hypothesized in animals and humans. No valid mechanism, however, has been proposed for an interaction between PF-MF and biological tissues and living beings at intensities relevant to animal and human exposure. Here we pr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20568
更新日期:2010-07-01 00:00:00
abstract::This paper presents a theoretical analysis of electromagnetic environments in power substations in service. A new analytical method is developed and applied to magnetic field calculations in 187-kV and 66-kV substations. Experimental results verify that the method offers a good prediction of the magnetic field profile...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250100106
更新日期:1989-01-01 00:00:00
abstract::Rats were tested for neurobehavioral alterations immediately after exposure to 2.45-GHz (CW) microwave radiation at 10 mW/cm2 for 7 h. Behavioral tests used were locomotor activity, startle to an acoustic stimulus and acquisition and retention of a shock-motivated passive avoidance task. Both horizontal and vertical c...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250090307
更新日期:1988-01-01 00:00:00
abstract::The use of prosthetic implants is increasing both in the United States and around the world and there is a concomitant rise in cases of biofilm-based, persistent infections that are quite serious and virtually impervious to antibiotic treatment. The development of alternate therapies that do not involve long term use ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20529
更新日期:2010-01-01 00:00:00
abstract::The extent of electromagnetic interference (EMI) from 16.7-Hz alternate current power lines in the human surface electrocardiogram (ECG) was evaluated. Results showed a direct linear correlation between mean EMI and magnetic induction of 5.8-21 microT on a railroad platform (electric field: 270 V/m). EMI inside a rail...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.20319
更新日期:2007-07-01 00:00:00
abstract::Several investigators have reported robust, statistically significant results that indicate that weak (approximately 1 microT) magnetic fields (MFs) increase the rate of morphological abnormalities in chick embryos. However, other investigators have reported that weak MFs do not appear to affect embryo morphology at a...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/(sici)1521-186x(1997)18:6<431::aid-bem5>3.
更新日期:1997-01-01 00:00:00
abstract::This paper investigates the influence of pulsed magnetic fields (PMFs) on amplitude of evoked, compound action potential (CAP) recorded from the segments of sciatic nerve in vitro. PMFs were applied for 30 min at frequency of 0.16 Hz and intensity of 15 mT. In confirmation of our previous reports, PMF exposure enhance...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.21917
更新日期:2015-07-01 00:00:00
abstract::We studied the influence of magnetic fields (MFs) and simulated solar radiation (SSR) on ornithine decarboxylase (ODC) and polyamines in mouse epidermis. Chronic exposure to combined MF and SSR did not cause persistent effects on ODC activity or polyamines compared to the animals exposed only to UV, although the same ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The influence of a pulsed magnetic field (PMF; sawtooth with 45-microseconds linear rise time and 5-microseconds decay time, peak strength of 15 microT, and frequency 20000 pps) [corrected] on the embryogenesis of CBA/S mice was investigated in five experiments based on a total of 707 exposed and 543 unexposed primigr...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250140303
更新日期:1993-01-01 00:00:00
abstract::Using a crossover experimental design, we evaluated our earlier findings that exposure to a 30 kV/m, 60 Hz electric field for 12 hours per day, 7 days per week for 6 weeks produced significant changes in the performance rates of social behaviors among young adult male baboons. In the crossover experiment, the former c...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130506
更新日期:1992-01-01 00:00:00
abstract::A retrospective study on reproductive hazards was performed among 542 employees at Swedish power plants. Questionnaires were answered by 89% of the employees. Data on pregnancies were checked by studying hospital case records. There was a statistically significant, decreased frequency of "normal" pregnancy outcome, al...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250040109
更新日期:1983-01-01 00:00:00
abstract::C57/B1 mice were exposed during pregnancy (gestation days 0-19) to a 20 kHz magnetic field (MF). The asymmetric sawtooth-waveform magnetic field in the exposed racks had a flux density of 15 microT (peak to peak). After 19 days, the exposure was terminated, and the mice were housed individually under normal laboratory...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250160409
更新日期:1995-01-01 00:00:00
abstract::The potential ability of radiofrequency electromagnetic radiation (RFR) in the microwave range to induce mutagenesis, chromosomal aberrations, and sister chromatid exchanges in mammalian cells is being explored in our laboratories. In addition, we have also been examining the ability of simultaneous exposure to RFR an...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250110206
更新日期:1990-01-01 00:00:00
abstract::Radio-frequency electromagnetic radiation (RFR) at 915 and 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. The dose-response relation is unusual, consisting of two power-density "windows" in which enhanced efflux occur...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250130407
更新日期:1992-01-01 00:00:00
abstract::Digital cellular telephones using the GSM (Global System for Mobile Communication) transmit information in bursts of microwaves. This pulsed transmitting mode causes the battery current and currents in the electronics of the apparatus to be pulsed. These pulsed currents produce corresponding pulsed magnetic fields nea...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::An experimental arrangement is described that maximized the dosimetric information that can be obtained during in vitro studies with ELF magnetic fields. The arrangement enables researchers to distinguish between a purely magnetic-field effect and one that also involves the electric fields and currents induced by the ...
journal_title:Bioelectromagnetics
pub_type: 杂志文章
doi:10.1002/bem.2250110306
更新日期:1990-01-01 00:00:00
abstract::We used exposure to microwaves from a global system for mobile communication (GSM) mobile phone (915 MHz, specific absorption rate (SAR) 37 mW/kg) and power frequency magnetic field (50 Hz, 15 muT peak value) to investigate the response of lymphocytes from healthy subjects and from persons reporting hypersensitivity t...
journal_title:Bioelectromagnetics
pub_type: 临床试验,杂志文章
doi:10.1002/bem.20103
更新日期:2005-04-01 00:00:00