Abstract:
:Translation of small interfering RNA (siRNA)-based approaches into practical therapeutics is limited because of lack of an effective and cell-specific delivery system. Herein, we present a new method of selectively delivering siRNA to dendritic cells (DCs) in vivo using CD40 siRNA-containing immunoliposomes (siILs) that were decorated with DC-specific DEC-205 mAb. Administration of CD40 siILs resulted in DC-specific cell targeting in vitro and in vivo. On treatment with CD40 siILs, the expression of CD40 in DCs, as well allostimulatory activity was inhibited. In vivo administration resulted in selective siRNA uptake into immune organs and functional immune modulation as assessed using a model antigen. In conclusion, this is the first demonstration of DC-specific siRNA delivery and gene silencing in vivo, which highlights the potential of DC-mediated immune modulation and the feasibility of siRNA-based clinical therapy.
journal_name
Bloodjournal_title
Bloodauthors
Zheng X,Vladau C,Zhang X,Suzuki M,Ichim TE,Zhang ZX,Li M,Carrier E,Garcia B,Jevnikar AM,Min WPdoi
10.1182/blood-2008-04-151191subject
Has Abstractpub_date
2009-03-19 00:00:00pages
2646-54issue
12eissn
0006-4971issn
1528-0020pii
blood-2008-04-151191journal_volume
113pub_type
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