Abstract：
:We carried out ligand binding experiments on membranes from rat brain cortical grey matter using radioiodinated beta/A4 8-17, with non-specific binding determined by the addition of 10 microM unlabelled peptide. Specific, reversible binding amounted to 60-75% of total binding and showed a clear dependence on time, temperature, pH and membrane concentration. Kinetic analyses indicated a high-affinity binding site with an apparent KD of 440 pM. However, the ligand was partly degraded with loss of the Ser8, Lys16 and Leu17 residues. Excision of the two C-terminal amino acids was inhibited by EDTA, EGTA, dithiothreitol or Zn2+ but was stimulated by Ca2+ or Mn2+. These studies demonstrate high-affinity binding sites for beta/A4 8-17 (or its derivatives) in rat brain, suggesting that this region may contain a physiologically important amino acid sequence and identify a potential membrane-associated amyloid precursor protein (APP) secretase activity.
journal_name
Brain Resjournal_title
Brain researchauthors
Allsop D,Yamamoto T,Kametani F,Miyazaki N,Ishii Tdoi
10.1016/0006-8993(91)90905-bsubject
Has Abstractpub_date
1991-06-14 00:00:00pages
1-9issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(91)90905-Bjournal_volume
551pub_type
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