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Expression of exogenous DNA in rat liver cells after liposome-mediated transfection in vivo.

Abstract：

:Liposomes containing the expression vector pRSVneo coding for neomycin phosphotransferase-II were used for a gene transfer into rat liver cells in vivo. After intravenous application of liposomes to male Wistar-rats, non-integrated vector DNA was detected by blot-hybridisation in isolated nuclei of hepatocytes. Presence of messenger RNA specific for the transferred construct was demonstrated by Northern analyses of poly(A)+RNA. The biological activity of the neomycin phosphotransferase-II gene and its enzyme could be demonstrated in hepatocytes for at least 7d post injection. Liposome-mediated gene transfer into adult animals is discussed as a model system to study the expression and fate of transferred genes in adult rat hepatocytes under physiological conditions.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Leibiger B,Leibiger I,Sarrach D,Zühlke H

doi

10.1016/0006-291x(91)91552-n

subject

Has Abstract

pub_date

1991-02-14 00:00:00

pages

1223-31

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91552-N

journal_volume

174

pub_type

杂志文章

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