Abstract:
:Full-field optical coherence tomography (FF-OCT) capable of in vivo cellular-level imaging is demonstrated for nonscanning horizontal cross-sectional imaging. The system is based on a white light interference microscope illuminated by a thermal light source. A dual-channel two-dimensional (2-D) detection technique incorporated with a pair of CCD cameras has been developed, where a pair of interferometric images with phase difference of pi/2 are simultaneously captured using an achromatic phase shifter. By acquiring an additional pair of images with a conventional phase shift method, a horizontal cross section is derived from every two consecutive CCD frames, enabling OCT imaging at the video rate. Using an ultrabroad bandwidth illumination incorporated with relatively high NA (0.8 NA) water immersion objectives, an axial resolution of 0.8 microm and a transverse resolution of 0.7 microm are experimentally confirmed. A field of view of 215 microm x 215 microm is covered by the 500 x 500 pixel CCD cameras. We demonstrate, for what is believed to be the first time, in vivo cellular-level blood flow imaging of a Xenopus laevis tadpole by FF-OCT.
journal_name
J Biomed Optjournal_title
Journal of biomedical opticsauthors
Akiba M,Chan KPdoi
10.1117/1.2822159subject
Has Abstractpub_date
2007-11-01 00:00:00pages
064024issue
6eissn
1083-3668issn
1560-2281journal_volume
12pub_type
杂志文章abstract::A computer simulation called RHME (Retinal Heating in Moving Eye) is developed to simulate the heating pattern that occurs in the retina during a long-duration exposure to a continuous wave laser beam. The simulation takes into account eye movements that occur during a deliberate fixation. Due to the rapid (millisecon...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1783355
更新日期:2004-09-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.23.9.091415
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abstract::Surgical excision of the whole prostate through a radical prostatectomy procedure is part of the standard of care for prostate cancer. Positive surgical margins (cancer cells having spread into surrounding nonresected tissue) occur in as many as 1 in 5 cases and strongly correlate with disease recurrence and the requi...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.2.025001
更新日期:2019-02-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2773728
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abstract::Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid le...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3497565
更新日期:2010-09-01 00:00:00
abstract::Diffuse optical spectroscopy (DOS) provides a powerful tool for fast and noninvasive disease diagnosis. The ability to leverage DOS to accurately quantify tissue optical parameters hinges on the model used to estimate light-tissue interaction. We describe the accuracy of a lookup table (LUT)-based inverse model for me...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.5.057001
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abstract::An integrated miniature multi-modal microscope (4M device) for microendoscopy was built and tested. Imaging performance is evaluated and imaging results are presented for both fluorescence and reflectance samples. Images of biological samples show successful imaging of both thin layers of fixed cells prepared on a sli...
journal_title:Journal of biomedical optics
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journal_title:Journal of biomedical optics
pub_type: 信件
doi:10.1117/1.JBO.23.10.100501
更新日期:2018-10-01 00:00:00
abstract::We present a novel Fourier-domain angle-resolved low-coherence interferometry (a /LCI) fiber probe designed for in vivo clinical application in gastrointestinal endoscopy. The a/LCI technique measures the depth-resolved angular scattering distribution to determine the size distribution and optical density of cell nucl...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3520130
更新日期:2011-01-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1344589
更新日期:2001-04-01 00:00:00
abstract::We describe a systematic approach to image, track, and quantify the movements of HIV viruses embedded in human cervical mucus. The underlying motivation for this study is that, in HIV-infected adults, women account for more than half of all new cases and most of these women acquire the infection through heterosexual c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.9.096001
更新日期:2016-09-01 00:00:00
abstract::Modern gynecologic practice requires noninvasive diagnostics techniques capable of detecting morphological and functional alterations in tissues of female reproductive organs. Optical coherence tomography (OCT) is a promising tool for providing imaging of biotissues with high resolution at depths up to 2 mm. Design of...
journal_title:Journal of biomedical optics
pub_type: 杂志文章,评审
doi:10.1117/1.JBO.22.12.121709
更新日期:2017-12-01 00:00:00
abstract::An approach to an established technique that is potentially applicable for a more comprehensive understanding of the electrical properties of red blood cells (RBCs) is presented. Using a high-intensity gradient laser trap, RBCs can be singly trapped and consequentially ionized. The subsequent dynamics of the ionized c...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.23.5.055005
更新日期:2018-05-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.21.11.116006
更新日期:2016-11-01 00:00:00
abstract::Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bioenvironment. The mesop...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3083427
更新日期:2009-01-01 00:00:00
abstract::Microscopic visualization of islets of Langerhans under normal and diabetic conditions is essential for understanding the pathophysiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-base...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3470241
更新日期:2010-07-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3147402
更新日期:2009-05-01 00:00:00
abstract::The recycling of G-protein-coupled receptors (GPCR) to the cell surface after internalization plays an important role in the regulation of overall GPCR activity. The angiotensin II type I receptor (AT1R) belongs to class B GPCRs that recycle slowly back to the cell surface. Previous studies have proposed that Rab11 co...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.2943286
更新日期:2008-05-01 00:00:00
abstract::We report a photonic approach for selective inactivation of viruses with a near-infrared subpicosecond laser. We demonstrate that this method can selectively inactivate viral particles ranging from nonpathogenic viruses such as the M13 bacteriophage and the tobacco mosaic virus to pathogenic viruses such as the human ...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.3275477
更新日期:2009-11-01 00:00:00
abstract::So far, the study of the optical clearing is almost always about healthy tissue. However, the ultimate goal is to detect diseases for clinical application. Optical clearing on diseased skins is explored. The effect is evaluated by applying a combined liquid paraffin and glycerol mixed solution on several kinds of dise...
journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.17.11.115003
更新日期:2012-11-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.19.8.080503
更新日期:2014-08-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.25.9.090502
更新日期:2020-09-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2020-10-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.JBO.24.10.106004
更新日期:2019-10-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
doi:10.1117/1.1899185
更新日期:2005-03-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Journal of biomedical optics
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00