Abstract:
:Glioblastoma multiform is the most common malignant primary brain tumor in adults, but there remains no effective therapeutic approach. 2-methoxyestradiol (2-ME), which is a naturally occurring metabolite of 17beta-estradiol, was shown to enhance radiotherapeutic effect in certain tumors; however, whether 2-ME can also enhance the sensitivity of glioma cells to radiotherapy remains unknown. The present study, therefore, was to address this issue using two human glioma cell lines (T98G and U251MG). These cells were irradiated with and without 2-ME and then clonogenic assay, apoptosis assay, DNA damage, and cell cycle change were examined. Results showed that 2-ME significantly enhances radiation-induced cell death in both glioma cells, shown by decreasing cell viability and increasing apoptotic cell death. No such radiosensitizing effect was observed if cells pre-treated with Estrodiol, suggesting the specifically radiosensitizing effect of 2-ME rather than a general effect of estrodials. The enhanced radio-cytotoxic effect in glioma cells by 2-ME was found to be associated with its enhancement of G(2)/M arrest and DNA damage, and phosphorylated ATM protein kinases as well as cell cycle checkpoint protein Chk2. Furthermore, inhibition of ATM by ATM inhibitor abolished 2-ME-activated Chk2 and enhanced radio-cytotoxic effects. These results suggest that 2-ME enhancement of the sensitivity of glioma cell lines to radiotherapy is mediated by induction of G2/M cell cycle arrest and increased DNA damage via activation of ATM kinases.
journal_name
Brain Resjournal_title
Brain researchauthors
Zou H,Zhao S,Zhang J,Lv G,Zhang X,Yu H,Wang H,Wang Ldoi
10.1016/j.brainres.2007.07.092subject
Has Abstractpub_date
2007-12-14 00:00:00pages
231-8eissn
0006-8993issn
1872-6240pii
S0006-8993(07)01943-9journal_volume
1185pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The link between the immunolocalization of apolipoproteins E (apo E) and J (apo J) and the different severity of beta-amyloid deposition in various areas of Alzheimer's disease (AD) and Down's syndrome (DS) brain was analyzed. Both apolipoproteins were found in all types of senile plaques (SPs) in the cerebral cortex,...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00482-6
更新日期:1995-07-10 00:00:00
abstract::The specific binding of [3H]neurotensin binding to the rat striatum was characterized and its localization investigated by using frontal cortex ablations and the neurotoxins kainic acid and 6-hydroxydopamine. Scatchard analysis indicated the existence of a single population of binding sites and the potencies of variou...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90801-1
更新日期:1984-05-07 00:00:00
abstract::The axons in the nerve fiber layer (NFL) of the adult rat retina were examined by transmission electron microscopy. NFL axons range in size from 0.12 to about 2.0 microm, with a peak at 0.3-0.4 microm. In addition to conventional small mitochondria in the NFL axons contain some large ones, which are similar to astrocy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)91222-2
更新日期:1983-01-03 00:00:00
abstract::Neural stem cells can be derived from the adult/embryonic nervous system as well as from more primitive embryonic stem cells but, because of the lack of specific markers, only their differentiated progeny can be characterized. We here report the presence of several endothelial and hematopoietic receptors (at protein a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)03291-7
更新日期:2002-01-25 00:00:00
abstract::Stanniocalcin (STC-1), a 50 kDa glycoprotein hormone that regulates calcium/phosphate homeostasis in bony fish and mammals, has been shown to be expressed in central neurons and choroid plexus, and to exert a protective effect against hypercalcemic and hypoxic damage to neurons. Circumventricular organs are known to f...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.04.070
更新日期:2008-07-07 00:00:00
abstract::During a simple reaction time task using auditory or visual stimuli, a total of 96 single units were recorded from the dorsolateral prefrontal cortex of macaque monkeys. These monkeys were trained to depress a lever for a fixed period which produced a tone burst or a small spot of light. After a variable period, the s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)91025-3
更新日期:1982-09-09 00:00:00
abstract::The sexual and associated behaviour of 10 adult male marmosets was recorded during pair tests with ovariectomized females, before and after bilateral thermal lesions of the hypothalamus. Four sham-lesioned males served as controls. Lesions varied in volume from 1.49 to 3.28 mm3 and extended from the ventromedial hypot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90405-2
更新日期:1988-11-01 00:00:00
abstract::The sites of action of lidocaine on the responses evoked by stimulation of lateral column (LC) and dorsal root (DR) were studied in the isolated, intra-arterially perfused spinal cord of the bullfrog. When the ventral root volley produced by stimulation was abolished by lidocaine, the presynaptic focal potential was a...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90172-x
更新日期:1982-10-07 00:00:00
abstract::The effect of lung inflation on the excitability of inspiratory neurons of the dorsal respiratory group was studied in decerebrate, paralyzed, artificially ventilated cats. Variations in the antidromic latency (AL) were used as a measure of the changes in excitability. The antidromic responses of single cells were rec...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91278-3
更新日期:1986-02-26 00:00:00
abstract::Histone proteins from purified nuclei of neonatal and adult mouse retinas were analyzed and compared utilizing SDS-polyacrylamide gel electrophoresis. Identical procedures were applied to examine the histones extracted from the brains of the same animals. In the newborn and mature retina and brain, 8 histone fractions...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90150-7
更新日期:1987-06-01 00:00:00
abstract::Median preoptic nucleus (POMe) neurons are innervated by catecholaminergic and neuropeptide Y (NPY)-immunoreactive nerve terminals originating from the catecholamine area of the ventrolateral medulla (VLM). The possibility that such POMe neurons project to the VLM catecholamine area was investigated in the rat. Immuno...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01240-8
更新日期:1999-04-17 00:00:00
abstract::1-(p-Bromobenzoyl)-piperazine-2,3-dicarboxylic acid (pBB-PzDA; 0.03-5 mM), an excitatory amino acid antagonist, was perfused through the guinea pig cochlea while monitoring various cochlear potentials. pBB-PzDA (1-5 mM) reversibly suppressed the amplitude of the compound action potential of the auditory nerve (CAP) an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90934-7
更新日期:1989-05-15 00:00:00
abstract::The involvement of endogenous serotonergic pathways in the mediation of antinociception has been indicated by electrophysiological, pharmacological and behavioral experiments. However, manipulation of the indole pathway, either by lesioning of raphe nuclei or drug intervention, often produces disparate results. In par...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90883-3
更新日期:1987-11-24 00:00:00
abstract::Our recent results showed that angiotensin II or III (AII, AIII) microinjected into the zona incerta (ZI) significantly increased water intake. The most effective doses of AII and AIII were also defined. The two neuropeptides had their effects differently on drinking via different receptors. AII bound to AT(1) that wa...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.01.002
更新日期:2004-03-26 00:00:00
abstract::Two-day-old rats were pretreated with 50 mg/kg of capsaicin. After 3--4 months, specific binding of [3H]muscimol and [3H]strychnine was measured in membrane preparations from dorsal spinal cord. A 20-30% decrease of the number of [3H]muscimol binding sites was observed after capsaicin treatment. In contrast, [3H]stryc...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90160-2
更新日期:1980-12-08 00:00:00
abstract::GM1 ganglioside injections (i.p.) reduce amphetamine-induced asymmetric rotation in rats 48 h after a partial unilateral transection of the nigrostriatal pathway. We found that this reduction was maximal when rats received their first GM1 injection within 2 h after surgery. Rats injected 4-12 h after surgery, or rats ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)90871-1
更新日期:1986-07-09 00:00:00
abstract::Mutations in two genes, alpha-synuclein and parkin, have been identified as some rare causes for familial Parkinson's disease (PD). alpha-Synuclein and parkin protein have subsequently been identified in Lewy bodies (LB). To gain further insight into the pathogenesis of PD we investigated the role of neurofilament lig...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03138-4
更新日期:2002-09-27 00:00:00
abstract::Golgi examination of neurons of self-stimulation areas of the lateral hypothalamus and substantia nigra-ventral tegmental areas of adult Wistar rats that had experienced self-stimulation for 10 days revealed a significantly higher number of dendritic branching points in the two self-stimulation areas, and also in the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91079-v
更新日期:1990-09-10 00:00:00
abstract::Neuropeptide Y (NPY) has been detected immunocytochemically in cerebral cortex and subcortical white matter of the primate frontal, parietal, temporal, and occipital lobes. Because little is known about NPY in the primate insular lobe and because peptides play an important role in normal neuronal functioning and alter...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91245-n
更新日期:1993-02-19 00:00:00
abstract::Rats with bilateral striatal cholinergic lesions induced by the neurotoxin AF64A were found to have increased spontaneous nocturnal locomotor activity as measured in terms of the amount of time spent moving, the total distance traveled and the number of episodes and time of stereotypic behaviors. No significant differ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90715-7
更新日期:1984-05-14 00:00:00
abstract::In anaesthetized, spontaneously breathing cats and dogs, extracellular recordings were made of the spontaneous activities of 28 single neurones with cardiac rhythm. It was confirmed by histological examination that the neurones were situated in the mediodorsal portion of the nucleus of the solitary tract. Different di...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)90049-x
更新日期:1977-09-09 00:00:00
abstract::Cultures of mouse spinal cord were used to visualize binding sites for [125I]angiotensin II (AII) by autoradiography. Visualization by light microscopy shows that neurones, but also glial cells possess angiotensin II binding sites which are located both on soma and processes. These findings open a new field of investi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91642-3
更新日期:1988-03-08 00:00:00
abstract::The effect of bombesin on the activity of dopamine (DA) neurons comprising the nigrostriatal, mesolimbic, tuberoinfundibular and tuberohypophysial systems in the male rat was determined by measuring: (1) the accumulation of 3,4-dihydroxyphenylalanine (DOPA) after administration of a decarboxylase inhibitor, and (2) th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91745-m
更新日期:1991-11-22 00:00:00
abstract::The subpallial region of the avian telencephalon contains neural systems whose functions are critical to the survival of individual vertebrates and their species. The subpallial neural structures can be grouped into five major functional systems, namely the dorsal somatomotor basal ganglia; ventral viscerolimbic basal...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2011.09.037
更新日期:2011-11-18 00:00:00
abstract::Neonatal (postnatal days 8-21) exposure of rats to the selective serotonin reuptake inhibitor (SSRI), citalopram, results in persistent changes in behavior including decreased sexual activity in adult animals. We hypothesized that this effect was a consequence of abnormal stimulation of 5-HT(1A) and/or 5-HT(1B) recept...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.10.009
更新日期:2006-12-13 00:00:00
abstract::The effects of the sulfated octapeptide of cholecystokinin (CCK8-S), the non-sulfated homolog (CCK8-NS) as well as the C-terminal di-, tri- and tetrapeptidic fragments (respectively CCK2, CCK3 and CCK4) were studied in vitro in rat hippocampal slices by extracellular recording of the spontaneous action potential disch...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90776-7
更新日期:1988-06-07 00:00:00
abstract::Using a double-labeling method that combines retrograde tracing and immunocytochemistry, we demonstrated the presence of a hippocampofugal gamma-aminobutyric acid (GABA)-like immunoreactive (GABA-LI) neuron system in the rat. As a preliminary experiment, knife-cut of the dorsal fornix was made and it resulted in a rem...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90757-8
更新日期:1987-04-14 00:00:00
abstract::Repeated odorant or electrical stimulation of the frog olfactory nerve leads to long-lasting reduction of excitability of the receptor neurons. In the turtle olfactory nerve electrical stimulation causes an increase in extracellular potassium concentration due to the efflux of potassium from active axons. Elevated pot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90340-h
更新日期:1990-12-24 00:00:00
abstract::Brain cortico-striatal circuits have consistently been implicated in the pathology of addiction related disorders. We applied a reliable seed-based analysis of the resting-state brain activity to comprehensively delineate the subdivisions of striatal functional connectivity implicated in internet gaming disorder. Amon...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2014.12.042
更新日期:2015-03-30 00:00:00
abstract::Voluntary saccades performed in total darkness provide the opportunity to investigate the brain system active during saccades without confounding effects caused by the saccade induced shifts of the retinal image. Using this approach saccade related activation has been demonstrated not only in parietal but also in stri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.07.108
更新日期:2008-10-03 00:00:00