Abstract：
:Lycium barbarum is an oriental medicinal herb that has long been used for its anti-aging and cell-protective properties. Previous studies have shown that aqueous extracts from L. barbarum exhibit neuroprotection via inhibiting pro-apoptotic signaling pathways. Other active components can also be accomplished by novel alkaline extraction method, which may give different profiles of water-soluble components. We hypothesize that another active component obtained by alkaline extraction method exerts different biological mechanisms to protect neurons. In this study, we aim to examine the neuroprotective effects from the alkaline extract of L. barbarum, namely LBB, to attenuate beta-amyloid (Abeta) peptide neurotoxicity. Primary cortical neurons were exposed to Abeta-peptides inducing apoptosis and neuronal cell death. Pretreatment of LBB significantly reduced the level of lactate dehydrogenase (LDH) release and the activity of caspase-3 triggered by Abeta. "Wash-out" procedures did not reduce its neuroprotective effects, suggesting that LBB may not bind directly to Abeta. We have further isolated three subfractions from LBB, namely LBB-0, LBB-I and LBB-II. LBB-I and LBB-II showed differential neuroprotective effects. Western blot analysis demonstrated that LBB-I and LBB-II markedly enhanced the phosphorylation of Akt. Taken together, our results suggested that the glycoconjugate isolated from novel alkaline extraction method can open up a new avenue for drug discovery in neurodegenerative diseases.
journal_name
Brain Resjournal_title
Brain researchauthors
Ho YS,Yu MS,Lai CS,So KF,Yuen WH,Chang RCdoi
10.1016/j.brainres.2007.04.075subject
Has Abstractpub_date
2007-07-16 00:00:00pages
123-34eissn
0006-8993issn
1872-6240pii
S0006-8993(07)01022-0journal_volume
1158pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:To elucidate the generation mechanisms of interictal spikes we analyzed the electrical events in the different layers of the rabbit's neocortex after application of penicillin to distinct cortical layers. Field potentials (FP) recorded simultaneously from all layers with a 16-fold micro-electrode-assembly were subject...
journal_title：Brain research
pub_type： 杂志文章
doi：
更新日期：1984-09-10 00:00:00
abstract：:NK1 receptor antagonists have been shown to act centrally and to produce a broad-spectrum anti-emetic action. To determine precisely the site of this action, we microinjected GR205171, an NK1 receptor antagonist, into the left medulla oblongata in decerebrate paralyzed dogs. The right medulla was transected 2.5 mm ros...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01324-9
更新日期：1999-02-13 00:00:00
abstract：:Nerve fibers containing 5-hydroxytryptamine (5-HT) were demonstrated immunohistochemically in the wall of pial vessels associated with the circle of Willis in the guinea pig. The fibers formed a network structure which was more dense in the rostral part of the arterial circle and its branches than in the caudal part. ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91342-x
更新日期：1988-01-12 00:00:00
abstract：:Focal electrical stimulation and glutamate microinjection in the nuclei reticularis gigantocellularis (NGC) and gigantocellularis pars alpha (NGC alpha) both inhibit the nociceptive tail-flick (TF) reflex in rats. The present experiments were undertaken to determine the transmitter(s) at the level of the lumbar spinal...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91825-2
更新日期：1990-12-03 00:00:00
abstract：:Recordings have been made from 162 pyramidal tract neurones which discharged bursts of nerve impulses in characteristic temporal association with performances of a stereotyped motor task by monkeys. Clinical evaluation of the relationship between discharges of the neurones and free movement led to the view that each c...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90507-7
更新日期：1975-11-07 00:00:00
abstract：:Effects of nitric oxide on glutamate (Glu) release in long-term potentiation (LTP) were investigated by superfusion of conventional (P2) and large (P3) synaptosomes prepared from the rat hippocampus. Basal releasing rates of endogenous Glu from P2 and P3 fractions were 103.6 and 85.2 pmol/min/mg protein, respectively....
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90355-7
更新日期：1994-04-25 00:00:00
abstract：:The research question was, do events arising from rhythmic passive movement of the human leg lead to inhibition of the H reflex pathway in the stationary leg contralateral to that movement? Further, as the angular velocity of the passive movement increases, does the contralateral reflex inhibition also increase? Stabl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91303-a
更新日期：1993-02-12 00:00:00
abstract：:Activation of cAMP-dependent protein kinase (kinase A) has recently been shown to enhance responses evoked by stimulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors in cultured hippocampal pyramidal neurons. Here we report results of experiments designed to determine if activation of the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90803-h
更新日期：1992-03-06 00:00:00
abstract：:We examined the effect of CP-154,526 on cardiovascular changes elicited in conscious rabbits by stressful stimuli (loud sound, cage move, pinprick, formaldehyde vapour and air-jet stress). CP-154,526 substantially reduced pressor and heart rate responses to these stimuli (both vagally and sympathetically mediated), an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.05.062
更新日期：2004-08-13 00:00:00
abstract：:In over half a century numerous studies have demonstrated that different odorants produce individually different spatial patterns of neural receptor activity on the olfactory mucosa. However, the thought that these differential activity patterns could be the neural code underlying olfactory perception has not been tes...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02512-5
更新日期：2003-08-15 00:00:00
abstract：:The effects of intracellular injection of TEA on the stretch-induced response of the slowly adapting stretch receptor of the crayfish have been examined to determine the contribution of an outward potassium current to the early adaptation of the neuron. Intracellular recording techniques including potential clamp meas...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90410-x
更新日期：1985-06-10 00:00:00
abstract：:The bed nucleus of the stria terminalis (BNST) receives dense noradrenergic projections from the brainstem and has been claimed to play a role in expression of a variety of stress responses. Fear-related stimuli suppress vasopressin and facilitate oxytocin release from the neurohypophysis and induce behavioral suppres...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00012-2
更新日期：1998-03-30 00:00:00
abstract：:We investigated c-Met expression in cultured astrocytes and their regulation by cytokines. Immunocytochemistry revealed that c-Met was expressed in cultured astrocytes. Western blotting revealed that acidic and basic fibroblast growth factor (FGF) enhanced and hepatocyte growth factor (HGF) reduced c-Met expression. R...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03975-6
更新日期：2003-02-07 00:00:00
abstract：:Brain activity was measured autoradiographically using [1-14C]octanoate (OCTO) as a fast functional tracer in rats receiving either saline, heroin or cocaine. Regional optical densities were normalized to a relative optical density index for comparisons of OCTO labeling between treatment groups. Heroin significantly i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91323-6
更新日期：1988-01-12 00:00:00
abstract：:Recent evidence indicates that the superior colliculus (SC), in addition to its functions in sensory detection, also participates in controlling the generalized activation state of the forebrain, as measured by the electroencephalogram (EEG) or electrocorticogram (ECoG). The mechanisms by which the SC modulates forebr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)04062-3
更新日期：2003-02-21 00:00:00
abstract：:Depolarizing actions of 4 conformationally restricted L-glutamate analogues, (2S,3S,4S) isomer (L-CCG-I), (2S,3R,4R) isomer (L-CCG-II), (2S,3S,4R) isomer (L-CCG-III) and (2S,3R,4S) isomer (L-CCG-IV) of L-alpha-(carboxycyclopropyl)-glycine (L-CCG), were investigated in the isolated rat spinal cord by extracellular reco...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90207-2
更新日期：1989-02-20 00:00:00
abstract：:Repeated amphetamine administration produced behavioral sensitization to subsequent amphetamine challenge. The development of sensitization was blocked by coadministration of the N-methyl-D-aspartate (NMDA) antagonist MK-801. Conditioned locomotion, as revealed by saline challenge, was also blocked by MK-801, suggesti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91202-c
更新日期：1991-10-18 00:00:00
abstract：:The present study has investigated the long-term changes in the organisation of the corticomotor projection to the hand in a group of subjects who had sustained a subcortical hemispheric stroke up to 15 years previously and had subsequently recovered normal or near-normal motor function. Transcranial magnetic cortical...
journal_title：Brain research
pub_type： 临床试验,杂志文章
doi：10.1016/s0006-8993(00)03089-4
更新日期：2001-01-19 00:00:00
abstract：:The present study investigated the effects of systemic administration of dexmedetomidine, a selective alpha2 adrenergic receptor (alpha2AR) agonist, and gabapentin either alone or in combination on thermal hyperalgesia evoked by ankle joint inflammation. Monoarthritis of rat ankle joint was induced by an intra-articul...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.01.029
更新日期：2009-04-06 00:00:00
abstract：:William James noted that memories could persist from minutes to weeks. This essay attempts to explain this variation by situating the explanation in the biochemistry of dendritic spines. Two outcomes are critical to generate the synaptic basis of memory: (1) the actin cytoskeleton in the spine must be degraded to perm...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.12.009
更新日期：2015-09-24 00:00:00
abstract：:The amplitude of anterior neocortical EEG slow-waves (0.5-4 Hz) measured during quiet waking, drowsy (WS) and synchronized sleep (S) states showed slow rhythmic oscillations in WS and S similar to those previously reported in midbrain reticular neurons (periods of 8-12 s). Abrupt changes in slow rhythms of unit discha...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90153-1
更新日期：1983-06-20 00:00:00
abstract：:The lateral geniculate nucleus (LGN) of a fetal rat was transplanted to the visual cortex (VC) of a neonatal rat. A current source-density analysis of field potentials and an intracellular study of neuronal responses were conducted in slice preparations by electrical stimulation of transplanted LGN and host VC. The re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90554-3
更新日期：1987-09-29 00:00:00
abstract：:The effects of different ionic media on Ca2+ uptake and release in isolated brain microsomes were investigated. KCl (100 mM) provided the best medium for Ca2+ uptake in the presence of ATP. The effect of myo-inositol 1,4,5-trisphosphate (IP3) on Ca2+ release was examined and was maximum at 0.2 microM. IP3-induced Ca2+...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90562-2
更新日期：1987-09-01 00:00:00
abstract：:Cell transplantation using ventral mesencephalic tissue is an experimental approach to treat Parkinson's disease. This approach is limited by poor survival of the transplants and the high number of dopaminergic neurons needed for grafting. Increasing the yield of dopaminergic neurons in donor tissue is of great import...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2017.05.015
更新日期：2017-08-01 00:00:00
abstract：:Glial cell line derived neurotrophic factor (GDNF) is a potent survival factor for several types of neurons. GDNF binds with high affinity to GDNF-family receptor alpha-1 (GFR alpha-1). This receptor is expressed in different areas of the brain, including the hippocampus and dentate gyrus. By using in situ hybridizati...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02682-2
更新日期：2000-09-22 00:00:00
abstract：:In continuation of earlier computer simulation studies 15 of the feedback inhibition exercised by the Golgi cells of the cerebellum, an attempt is made at modeling the behavior of Purkinje and basket cells under somewhat more complex functional circumstances. The simulation study is based on a realistic network model ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(74)90531-9
更新日期：1974-03-15 00:00:00
abstract：:To determine the contribution of presynaptic cholinergic mechanisms to the increased sensitivity of a genetically selected line of Sprague-Dawley rats (Flinders S-line) to the anticholinesterase, diisopropyl fluorophosphate (DFP), rats were sacrificed by focused microwave irradiation of the head 1 min after a pulse in...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)91044-8
更新日期：1984-03-05 00:00:00
abstract：:Almost anyone who has ever lost weight can attest that it is harder to sustain weight loss than to lose weight. Maintenance of a 10% or greater reduced body weight is accompanied by decreases in energy expenditure to levels significantly below what is predicted solely on the basis of weight and body composition change...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2010.05.062
更新日期：2010-09-02 00:00:00
abstract：:Unilateral injections of 1 microgram of kainic acid in the archistriatum of 5-day-old domestic chicks reduced fear, as expressed by distress calls (peeps), significantly more than did identical injections in the same structure on the left side. This result adds affective behavior to the growing list of functions that ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90861-9
更新日期：1986-07-09 00:00:00
abstract：:The retinotopic organization of the expanded projection to the ipsilateral colliculus was studied electrophysiologically in 12 adult albino rats with one eye removed at birth. For comparison, the contralateral projection was rather variable in the representation of the nasotemporal axis of the retina along the rostroc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90200-2
更新日期：1984-11-12 00:00:00
