Abstract：
:The tissue-specific translation elongation factor eEF1A2 is a potential oncogene that is overexpressed in human ovarian cancer. eEF1A2 is highly similar (98%) to the near-ubiquitously expressed eEF1A1 (formerly known as EF1-alpha) making analysis with commercial antibodies difficult. We wanted to establish the expression pattern of eEF1A2 in ovarian cancer of defined histological subtypes at both the RNA and protein level, and to establish the mechanism for the overexpression of eEF1A2 in tumours. We show that while overexpression of eEF1A2 is seen at both the RNA and protein level in up to 75% of clear cell carcinomas, it occurs at a lower frequency in other histological subtypes. The copy number at the EEF1A2 locus does not correlate with expression level of the gene, no functional mutations were found, and the gene is unmethylated in both normal and tumour DNA, showing that overexpression is not dependent on genetic or epigenetic modifications at the EEF1A2 locus. We suggest that the cause of overexpression of eEF1A2 may be the inappropriate expression of a trans-acting factor. The oncogenicity of eEF1A2 may be related either to its role in protein synthesis or to potential non-canonical functions.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Tomlinson VA,Newbery HJ,Bergmann JH,Boyd J,Scott D,Wray NR,Sellar GC,Gabra H,Graham A,Williams AR,Abbott CMdoi
10.1038/sj.bjc.6603748subject
Has Abstractpub_date
2007-05-21 00:00:00pages
1613-20issue
10eissn
0007-0920issn
1532-1827pii
6603748journal_volume
96pub_type
杂志文章abstract：BACKGROUND:The overexpression of oestrogen-related receptor-β (ERRβ) in breast cancer patients is correlated with improved prognosis and longer relapse-free survival, and the level of ERRβ mRNA is inversely correlated with the S-phase fraction of cells from breast cancer patients. METHODS:Chromatin immunoprecipitation...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.53
更新日期：2014-04-15 00:00:00
abstract：:The growth of s.c. Walker 256 carcinoma was found to be independent of secondary growths induced by i.v. injection. Tumour cells injected i.v. lodged mainly in the lungs, with small clusters of cells in the lymph nodes. The rate of cellular proliferation of these secondary growths of Walker carcinoma was significantly...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.12
更新日期：1978-01-01 00:00:00
abstract：:The p53 tumour suppressor protein inhibits malignant progression by mediating cell cycle arrest, apoptosis or repair following cellular stress. One of the major regulators of p53 function is the MDM2 protein, and multiple forms of cellular stress activate p53 by inhibiting the MDM2-mediated degradation of p53. Mutatio...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1054/bjoc.2001.2128
更新日期：2001-12-14 00:00:00
abstract：BACKGROUND:Circulating total cytokeratin 18 (tCK18) and/or caspase cleaved cytokeratin 18 (cCK18) (measured by M65 and M30 enzyme-linked immunosorbent assays (ELISAs), respectively) are used as pharmacodynamic (PD) biomarkers of epithelial cell death in clinical trials. Having validated these ELISAs, we assessed their ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.416
更新日期：2012-10-23 00:00:00
abstract：:Lines of fibroblasts have been established from normal individuals and from patients diagnosed as suffering from one of the mucopolysaccharidoses or mucopolysaccharide-storage diseases. Transformation of these lines with SV40 virus has been found to reduce their capacity to secrete sulphated mucopolysaccharides into t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.156
更新日期：1977-07-01 00:00:00
abstract：BACKGROUND:Hepatocellular carcinoma (HCC) is one of the most common types of malignant tumour and has poor prognosis. Currently, systematic chemotherapy is the only approach to prolong survival. Thus the development of new treatment regimens is urgently needed to improve the therapeutic efficacy. Our study intended to ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.55
更新日期：2017-04-11 00:00:00
abstract：BACKGROUND:A dose-dense strategy has been considered to improve results of adjuvant chemotherapy for breast cancer. This randomised phase II trial investigated the feasibility of this approach with sequential anthracyclines and taxanes-based chemotherapy. METHODS:Patients with high-risk node-positive breast cancer wer...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2011.414
更新日期：2011-11-08 00:00:00
abstract：:We examined the expression of the midkine (MK) and alpha-fetoprotein (AFP) genes in 15 paired human specimens obtained from hepatocellular carcinoma (HCC) and the corresponding noncancerous regions of the same patients. A total of 14 HCC but none of the noncancerous specimens were positive for the MK mRNA. In contrast...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601246
更新日期：2003-09-15 00:00:00
abstract：BACKGROUND:Systemic inflammation measured by the neutrophil-to-lymphocyte ratio (NLR), leucocyte-to-lymphocyte ratio (LLR), platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR) and CRP/albumin ratio (CRP/Alb) was shown to impact the survival prognosis in patients with extracranial solid cancer. METHO...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-01254-0
更新日期：2021-01-21 00:00:00
abstract：:Human topoisomerase II enzymes are targets for a number of widely used anticancer agents. We have analysed a lung adenocarcinoma cell line CALU3, which has co-amplified topoisomerase II alpha and ERBB2 sequences, for the structure of the amplicon and for expression of both topoisomerase II alpha and beta. The region o...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.430
更新日期：1993-10-01 00:00:00
abstract：:Interleukin 6 (IL-6) and its soluble receptor (IL-6sR) can markedly stimulate aromatase activity in cultured fibroblasts derived from normal or malignant breast tissues. IL-6 acts by binding to a low-affinity membrane-spanning receptor (IL-6R), which must associate with a high-affinity receptor (gp130) for signal tran...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600785
更新日期：2003-02-24 00:00:00
abstract：:The aim of the study was to investigate whether 99Tc(m)-MIBI (Cardiolite), recently shown to be a substrate for P-glycoprotein, has the potential to be used as a marker for mdr1 gene expression and whether cyclosporin A (CyA) can modify its accumulation in vivo. Leukaemic cells from ten patients with acute myelocytic ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.290
更新日期：1998-06-01 00:00:00
abstract：:The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1984.32
更新日期：1984-02-01 00:00:00
abstract：:Malignancy of neoplasms is partly dependent on angiogenesis. Angiotensin II mediates angiogenesis and transcription of growth-related factors through stimulation of the AT1 receptor (AT1R). Losartan, a drug used mostly for treatment of hypertension, binds strongly to this receptor. We found the presence of AT1 recepto...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2001.2102
更新日期：2001-11-02 00:00:00
abstract：BACKGROUND:Head and neck squamous cell carcinoma (HNSCC) patients frequently develop treatment resistance to cetuximab, a monoclonal antibody against EGFR, as well as radiotherapy. Here we addressed extracellular signal-regulated kinase 1/2 (ERK1/2) regulation by cetuximab or fractionated irradiation (IR) and conducted...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-0892-9
更新日期：2020-07-01 00:00:00
abstract：:5'-nucleotidase activity, arachidonate metabolism and adenosine uptake were measured in P388 murine leukaemia cells and in a subline resistant to doxorubicin. These membranes related activities were found to be increased in the doxorubicin resistant cell line, compared to the drug sensitive cells. It is suggested that...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1984.71
更新日期：1984-04-01 00:00:00
abstract：:Eighteen consecutive patients with measurable locally advanced or metastatic pancreatic adenocarcinoma were treated with goserelin (Zoladex) 3.6 mg subcutaneously every 4 weeks. Hydrocortisone 20 milligrams twice daily was commenced with the second injection of goserelin. Objective tumour response was monitored by com...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1993.69
更新日期：1993-02-01 00:00:00
abstract：:Bispecific antibodies (bsAb) are considered as promising tools for the elimination of disseminated tumour cells in a minimal residual disease situation. The bsAb-mediated recruitment of an immune effector cell in close vicinity of a tumour cell is thought to induce an antitumoural immune response. However, classical b...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1054/bjoc.2000.1237
更新日期：2000-07-01 00:00:00
abstract：:Cultured cells maintained in very low oxygen levels alter their structure, metabolism and genetic expression. Culture conditions for cells were modified to minimise variation of nutrients and to allow normal survival levels after 24 h of hypoxic exposure. Under these hypoxic conditions, glucose consumption and lactate...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.264
更新日期：1990-08-01 00:00:00
abstract：:To assist in the description of the cellular heterogeneity present in normal and neoplastic urothelium, a panel of monoclonal antibodies (MoAbs) was raised against human transitional cell carcinoma (TCC) of the urinary bladder. All immunizations were carried out using whole cells and membrane preparations from well di...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1987.220
更新日期：1987-10-01 00:00:00
abstract：:Induction of lipolysis in murine white adipocytes, and stimulation of adenylate cyclase in adipocyte plasma membranes, by a tumour-produced lipid mobilizing factor, was attenuated by low concentrations (10(-7)--10(-5)M) of the specific beta3-adrenoceptor antagonist SR59230A. Lipid mobilizing factor (250 nM) produced c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600086
更新日期：2002-02-01 00:00:00
abstract：:Piwi proteins and their interaction with piRNAs have rapidly emerged as important contributors to gene regulation, indicating their crucial function in germline and stem cell development. However, data on the Hiwi 1 (Hiwi) gene, one of the four human Piwi homologues, are still scarce. Therefore, we investigated the Hi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604653
更新日期：2008-10-07 00:00:00
abstract：:Both misonidazole (MISO) and hyperthermia are known to enhance the radiation response of hypoxic cells, and to be selectively cytotoxic against cells in a hypoxic and acidic environment. The ability of these conditions to modify the effect of irradiation and their individual relationship was studied in a C3H mammary c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.2
更新日期：1980-01-01 00:00:00
abstract：:Apurinic/apyrimidinic (AP) sites in DNA are potentially lethal and mutagenic. They can arise spontaneously or following DNA damage from reactive oxygen species or alkylating agents, and they constitute a significant product of DNA damage following cellular exposure to ionizing radiation. The major AP endonuclease resp...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6690447
更新日期：1999-06-01 00:00:00
abstract：:Sixteen chronic myeloid leukaemia (CML) patients in remission were tested with solubilized membrane antigens from CML leukaemic cells, CML blasts, AML blasts and ALL blasts for cellular immunity in vitro by lymphocyte transformation (LT) and leucocyte migration inhibition (LMI) assays. Twelve CML patients in remission...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1979.193
更新日期：1979-09-01 00:00:00
abstract：:Double stranded RNA (dsRNA) whether isolated from a fungal virus or prepared synthetically (i.e., Poly I Poly C) and endotoxin were found to exert very similar effects on syngeneic murine lymphomata and fibrosarcomata. They cause complete regressions of some established subcutaneous (s.c.) or intradermal (i.d.) tumour...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1973.45
更新日期：1973-05-01 00:00:00
abstract：:Preclinical studies have demonstrated the synergistic anti-tumour activity of combination therapy with the oral cytostatics, 5'-deoxy-5-fluorouridine (5'-DFUR) and cyclophosphamide (CPA), in human breast cancer xenograft models. This study was performed to evaluate the efficacy and safety of this oral combination chem...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/sj.bjc.6601350
更新日期：2003-11-03 00:00:00
abstract：BACKGROUND:Our previous laboratory and clinical data suggested that one mechanism underlying the development of platinum resistance in ovarian cancer is the acquisition of DNA methylation. We therefore tested the hypothesis that the DNA hypomethylating agent 5-aza-2'-deoxycytodine (decitabine) can reverse resistance to...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/bjc.2014.116
更新日期：2014-04-15 00:00:00
abstract：:We screened 50 glioblastomas for P53 mutations. Five glioblastomas showed heterozygous mutations, while three were putatively heterozygous. Six of these eight glioblastomas showed elimination of wild-type P53 mRNA. These results strongly suggest that some sort of mechanism(s) favouring mutated over wild-type P53 mRNA ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604258
更新日期：2008-04-22 00:00:00
abstract：BACKGROUND:There is a need for sensitive and specific blood-borne markers for the detection of gastric cancer. Raised serum macrophage inhibitory factor (MIF) levels have been proposed as a marker for gastric cancer diagnosis but, to date, studies have only encompassed patients from high-incidence areas. METHODS:We ha...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2012.405
更新日期：2012-10-23 00:00:00
