Abstract:
:We investigated the effect of beta-estradiol (E2) on synaptogenesis in the hippocampus using organotypic hippocampal slice cultures and subregional hippocampal neuron cultures. E2 increased the expression of PSD95, a postsynaptic marker, specifically in stratum lucidum of Cornu Ammonis 3 (CA3SL) in cultured hippocampal slices. E2 also increased the spine density at the proximal site of CA3 apical dendrites in CA3SL and PSD95 was clustered on these spine heads. The effects of E2 on the expression of PSD95 and the spine density disappeared when the dentate gyrus (DG) had been excised at 1 day in vitro (DIV). FM1-43 analysis of subregional hippocampal neuron cultures which were comprised of Ammon's horn neurons, DG neurons, or a mixture of these neurons, revealed that E2 increased the number of presynaptic sites in the cultures that contained DG neurons. K252a, a potent inhibitor of the high affinity receptor of brain-derived neurotrophic factor (BDNF), and function-blocking antibody to BDNF (BDNFAB) completely inhibited the effects of E2 in hippocampal slice cultures and subregional neuron cultures, whereas ICI182,780 (ICI), a strong antagonist of nuclear estrogen receptors (nERs), did not. Expression of BDNF in DG neurons was markedly higher than that in Ammon's horn neurons and E2 did not affect these expression levels. E2 significantly increased the BDNF release from DG neurons. KT5720, a specific inhibitor of 3'-5'-cyclic adenosine monophosphate (cAMP)-dependent protein kinase A (PKA), and Rp-adenosine 3', 5'-cyclic monophosphorothioate triethylammonium salt (Rp-cAMP), a non-hydrolyzable diastereoisomer and a potent inhibitor of PKA, completely suppressed the E2-induced increase in BDNF release, whereas ICI and U0126, a potent inhibitor of MAP kinase kinase (MEK), did not. These results suggest that E2 induces synaptogenesis between mossy fibers and CA3 neurons by enhancing BDNF release from DG granule cells in a nER-independent and PKA-dependent manner.
journal_name
Brain Resjournal_title
Brain researchauthors
Sato K,Akaishi T,Matsuki N,Ohno Y,Nakazawa Kdoi
10.1016/j.brainres.2007.02.093subject
Has Abstractpub_date
2007-05-30 00:00:00pages
108-20eissn
0006-8993issn
1872-6240pii
S0006-8993(07)00502-1journal_volume
1150pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::In order to study the effects of treatment with monosodium glutamate (MSG) during the neonatal period on the intrinsic circadian timekeeping system in rats, the locomotor activity of blinded MSG-treated and control (saline-treated) rats was analyzed with power spectral analysis and cross-correlation. In contrast to a ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90084-8
更新日期:1985-07-29 00:00:00
abstract::Alpha-synuclein is a brain presynaptic protein that is linked to familiar early onset Parkinson's disease and it is also a major component of Lewy bodies in sporadic Parkinson's disease and other neurodegenerative disorders. Alpha-synuclein expression increases in substantia nigra of both MPTP-treated rodents and non-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02422-8
更新日期:2002-05-10 00:00:00
abstract::To gain greater insight on the molecular mechanisms that underlie ischemic stroke, we compared gene expression profiles in transient (tMCAO) and permanent middle cerebral artery occlusion (pMCAO) stroke models using Expression Analysis Systematic Explorer (EASE) pathway analysis software. Many transcripts were induced...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.11.090
更新日期:2006-02-03 00:00:00
abstract::Intradentate administration of colchicine has been reported to affect the cholinergic muscarinic system in the hippocampus, causing a reduction in quinclidinylbenzilate binding sites and an increase in choline acetyltransferase activity. Since the cholinergic muscarinic system is coupled to the formation of inositolph...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91419-4
更新日期:1989-01-16 00:00:00
abstract::The aim of this work was to study whether the vestibular afferent fibers establish direct connections with the motoneurons of glossopharyngeal and vagus nerves of the frog, Rana esculenta. In anaesthetized animals the vestibulocochlear nerve and the common root of glossopharyngeal-vagus and accessory (IX-X-XI) nerves ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.06.048
更新日期:2009-08-25 00:00:00
abstract::Examination of glycolmethacrylate embedded olfactory bulbs of normal rats revealed that the granule cells of the accessory olfactory bulb were dissimilar from the majority of main olfactory bulb granule cells. Cells from these structures can be discriminated on the basis of spherical form. nuclear diameter, nucleoplas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90711-9
更新日期:1982-03-25 00:00:00
abstract::Malignant glioma cells maintain an elevated intracellular pH (pH(i)) within hypoxic-ischemic tumor microenvironments through persistent activation of sodium-proton transport (McLean et al., 2000). Amiloride has been reported to selectively kill human malignant glioma cell lines but not primary astrocytes (Hegde et al....
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.09.059
更新日期:2010-12-02 00:00:00
abstract::Fasting induced a reduction in neuronal nitric oxide synthase (nNOS) mRNA in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of rats. The effect of intracerebroventricular (i.c.v.) administration of leptin on the nNOS mRNA level in the PVN and SON of fasting rats was studied by in situ hybridization his...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02065-x
更新日期:1999-11-06 00:00:00
abstract::The aim of this study was to assess the effect of Alzheimer's disease has on the functional integrity of several signal transduction proteins. The relative levels of the G-protein alpha subunits Gs alpha-L, Gs alpha-S, Gi alpha-2 and G(o) alpha were measured by western blotting and found to be unchanged in membranes p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90798-r
更新日期:1993-09-17 00:00:00
abstract::The roles of endogenous serotonin (5-HT) and 5-HT receptor subtypes in regulation of acetylcholine (ACh) release in frontal cortex of conscious rats were examined using a microdialysis technique. Systemic administration (1 and 3 mg/kg, i.p.) of the 5-HT-releasing agent p-chloroamphetamine (PCA) elevated ACh output in ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00856-1
更新日期:1997-10-24 00:00:00
abstract::Calretinin has been identified as a brain specific calcium-binding protein which appears as a prominent protein in the cochlear nucleus. We identified and localized calretinin in the guinea pig and rat inner ear using polyclonal antibodies. Immunoblot analyses of guinea pig and rat auditory nerve homogenates revealed ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91224-o
更新日期:1991-09-27 00:00:00
abstract::Autoradiographic techniques were used to test if positive modulators of AMPA-type glutamate receptors have regionally differentiated effects on ligand binding. Cyclothiazide, a drug with ten fold greater effects on 'flip' than 'flop' splice variants of the receptors, had unequal effects across the subdivisions of hipp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01315-2
更新日期:1998-02-02 00:00:00
abstract::Intermediate filament aggregation within motor neurons is a hallmark of ALS pathogenesis. Changes to intermediate filament stoichiometry due to altered mRNA steady-state levels of NEFL, PRPH and INA is thought to drive protein aggregation, yet the exact cause of these changes is unknown. MicroRNAs (miRNAs)-master regu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.10.032
更新日期:2019-03-01 00:00:00
abstract::When rat striatal membranes, photolabeled with [3H]dopamine under assay conditions similar to those used for dopamine-sensitive adenylate cyclase, were subjected to sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, several radioactively labeled bands appeared. Labeling of these bands was reduced in the ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90426-1
更新日期:1981-12-28 00:00:00
abstract::To determine whether the paraventricular nucleus (PVN) contributes to the development of hypertension in spontaneously hypertensive rats (SHR), we compared cardiovascular responses to ganglionic blockade with hexamethonium or vasopressin antagonism with dPVAVP in sham-operated or PVN lesioned SHR and Wistar-Kyoto rats...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90040-3
更新日期:1991-03-15 00:00:00
abstract::Rats trained to walk in a moving belt apparatus were subjected to a partial (fornix-fimbria (FF] or total (fornix-fimbria + cingulum bundles (FF + CB] chemical denervation of the dorsal serotonergic afferent pathways to the hippocampus. After chronic alcohol treatment that resulted in tolerance development to the moto...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)90037-x
更新日期:1985-02-11 00:00:00
abstract::The present study was undertaken to examine the differential effect of estrogen (E) on the expression of tyrosine hydroxylase (TH) in the substantia nigra compacta (SNc) and in two subdivisions of the ventral tegmental area in ovariectomized (ovx) and ovx plus estradiol benzoate (ovx+E)-treated female rats. Cell count...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02323-4
更新日期:2000-06-23 00:00:00
abstract::Incubation of rat striatal synaptosomes with the adenosine receptor agonist 2-chloroadenosine (2-CADO) produced a concentration-dependent increase of dopamine (DA) synthesis (about 50% of control value). The effect was not additive with the stimulation produced by either 10 microM forskolin or 2 mM dibutyryl cyclic AM...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90232-6
更新日期:1988-07-26 00:00:00
abstract::Labyrinthine-intact guinea pigs received unilateral, brainstem cannula injections of (1) 2.5 micrograms of the selective dihydropyridine L-type Ca2+ channel agonist, Bay K 8644 (n = 4 animals); (2) 10 micrograms Bay K 8644 (n = 4); 12.5 micrograms of the selective dihydropyridine L-type Ca2+ channel antagonist, nifedi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90677-f
更新日期:1993-12-10 00:00:00
abstract::The astrocyte-derived protein S100B stimulates production of inducible nitric oxide synthase and nitric oxide (NO) in astrocytes [Hu et al., 1996, J. Biol. Chem. 271:2543], but its effect on microglia is not known. In addition, S100B's ability to modulate the activity of other glial activating agents has not been defi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02251-9
更新日期:2000-01-17 00:00:00
abstract:BACKGROUND:Inflammatory damage aggravates the cerebral ischemic pathological process and may pave a new way for treatment. Bicyclol has been proved to elicit a series of biologic effects through its anti-inflammatory property in treating hepatitis and hepatic ischemic/reperfusion injury. Whether this protective effect ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2013.06.032
更新日期:2013-08-28 00:00:00
abstract::The postnatal development of cat triceps surae gamma-motoneurones, retrogradely labelled with horseradish peroxidase (HRP), was studied light and electron microscopically. The mean diameter of the cell bodies of the gamma-motoneurones increased by about 25% from birth to the adult stage, which was much less than the i...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90251-3
更新日期:1987-12-15 00:00:00
abstract::The effect of intrathecal (i.t.) galanin (GAL) on the prolonged increase of spinal reflex excitability produced by conditioning stimulation (CS) of unmyelinated muscle afferents was studied in decerebrate, spinalized, unanesthetized rats. A CS train (1 Hz, 20 s) applied to the unmyelinated fibers in the gastrocnemius ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91037-2
更新日期:1991-02-15 00:00:00
abstract::We previously reported that brain angiotensin II type 2 (AT2) receptors contribute to the hyperthermia induced by intrahypothalamic (intrapreoptic (i.p.o.)) administration of prostaglandin E2 (PGE2) in rats. The present study was carried out to investigate the role of angiotensin II (ANG II) receptors in the cardiovas...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02218-0
更新日期:2000-01-03 00:00:00
abstract::Clinically, treatment options where fetal distress is anticipated or identified are limited. Allopregnanolone is an endogenous steroid, that positively modulates the GABA(A) receptor, and that has anti-apoptotic and anti-excitotoxic actions, reducing brain damage in adult animal models of brain injury. We sought to de...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.11.035
更新日期:2012-01-18 00:00:00
abstract::The levels of PLCgamma, a phospholipase C (PLC) isozyme, were higher in the cytosol fraction than in the membrane fraction in several control brain regions. The levels of PLCgamma were significantly elevated in the membrane, but not in the cytosolic fraction of the hippocampus of AD subjects. In the superior and middl...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(98)00935-4
更新日期:1998-11-16 00:00:00
abstract::Alterations in protein phosphorylation may be important in the pathogenesis of Alzheimer's disease and recent observations suggest that a subset of protein kinase pathways may be selectively altered. Calcium/calmodulin-dependent protein kinase II CaM kinase II) is the most abundant protein kinase in the brain and is b...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)90979-2
更新日期:1994-09-19 00:00:00
abstract::gamma-Aminobutyric acid (GABA) synthesis in the brain is mediated by two major isoforms of glutamic acid decarboxylase, GAD(65) and GAD(67). The contribution of these isoforms to GABA synthesis flux (V(GAD)) is not known quantitatively. In the present study we compared V(GAD) in cortex of control and vigabatrin-treate...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02778-0
更新日期:2001-09-28 00:00:00
abstract::Experiments were performed which demonstrate that low power, pulsed microwave exposure induces a significant increase in local cerebral blood flow within the brain of the conscious rat. Previous studies purporting to examine changes in brain vascular permeability induced by microwave radiation must be re-evaluated in ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90668-5
更新日期:1981-01-05 00:00:00
abstract::Due to the structural similarity to N-methyl-4-phenyl pyridinium (MPP(+)), paraquat might induce dopaminergic toxicity in the brain. However, its blood--brain barrier (BBB) penetration has not been well documented. We studied the manner of BBB penetration and neural cell uptake of paraquat using a brain microdialysis ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(01)02577-x
更新日期:2001-07-06 00:00:00