Abstract：
:This report describes the development of a species testing system based on the diversity of nucleotide sequences in mitochondrial DNA (mtDNA) among species. Five species, human, cow, pig, dog, and cat, were considered. The partial nucleotide sequences in 16S ribosomal RNA coding region were chosen as the target for discriminating the species. The sequence diversities of this approximately 400 bp long region ranged from 15.7 to 24.1% among the five species. Sequencing analysis of this target on 50 individuals of each species (53 for dogs) revealed that the nucleotide sequences were well preserved within species. Species-specific PCR for each species was also designed, and satisfactory results with regard to both sensitivity and specificity were obtained. A validation study with DNA extracted from bovine bone exposed to the environment revealed that the PCRs designed in this study worked correctly. From the results obtained, it was shown that this testing system could be a good tool for species identification. One successful case report is also demonstrated.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Imaizumi K,Akutsu T,Miyasaka S,Yoshino Mdoi
10.1007/s00414-006-0127-5subject
Has Abstractpub_date
2007-05-01 00:00:00pages
184-91issue
3eissn
0937-9827issn
1437-1596journal_volume
121pub_type
杂志文章abstract：:Urine samples from 20 male volunteers of European Caucasian origin were stored at 4 degrees C over a 4-month period in order to compare the identification potential of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) markers. The amount of nDNA recovered from urines dramatically declined over time. Consequently, nDNA ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-005-0004-7
更新日期：2006-03-01 00:00:00
abstract：:The case of a man's death as a consequence of an accident with a mountain bike was examined. Despite only slight external injuries, a general examination revealed the existence of a rupture of the diaphragm which was the ultimate cause of death. The discussion reconstructs the way the accident occurred and briefly ana...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100237
更新日期：2001-12-01 00:00:00
abstract：:Medico-legal experts are increasingly enlisted to assess the methamphetamine and amphetamine serum concentrations after a criminal offense. However, since criminal users rarely provide useful information to medico-legal experts regarding the substances abused, when the substance(s) was/were used, dose of ingestion too...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1607-5
更新日期：2017-09-01 00:00:00
abstract：:Immunohistochemical investigation of the respiratory tract and lungs of 63 fire victims revealed a statistically significant enhanced expression of heat-shock protein 70 (Hsp70) in the epiglottis, the trachea, and the main and the peripheral bronchi compared with a control group. In the fire victims, a strong expressi...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-006-0105-y
更新日期：2006-11-01 00:00:00
abstract：:Genetic diversity indices and forensic parameters at 20 autosomal short tandem repeat (STR) loci were evaluated in 541 unrelated individuals of north-western Indian state Rajasthan. In total, 243 alleles were observed with an average of 12.15 alleles per locus. The most polymorphic and discriminative locus was Penta E...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02330-z
更新日期：2020-06-05 00:00:00
abstract：:Projectile impact in sandwich bones typically results in formation of conoidal wounds exhibiting a larger region of damage on the inner cortical plate termed the bevel. To date, a number of hypotheses have been put forward to explain the formation of this wound type. The plug and spall hypothesis suggests that the con...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02086-1
更新日期：2019-09-01 00:00:00
abstract：:Severe cold stress has been shown to cause changes in the expression and secretion of thrombomodulin (TM), an endothelial protein regulating haemostasis and inflammation. To further evaluate TM as a cold stress indicator, relative TM mRNA and TM protein levels in the myocardium and the concentrations of TM in serum an...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1138-2
更新日期：2015-03-01 00:00:00
abstract：:The localization of ABH related antigens in human male reproductive tract was examined using monoclonal antibodies and an avidin biotin complex method. No positive reaction with blood group antibodies on spermatozoa was observed in testis and ductus epididymidis apart from erythrocytes and endothelial cells. The expre...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF02340827
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:The aim of this study was to evaluate the sensitivity of postmortem computed tomography (PMCT), postmortem magnetic resonance imaging (PMMR) and PMCT angiography (PMCTA) compared with autopsy in cases of adult death investigations. METHODS:For this systematic review and meta-analysis, Embase, PubMed, Scopus...
journal_title：International journal of legal medicine
pub_type： 杂志文章,meta分析
doi：10.1007/s00414-019-02140-y
更新日期：2020-01-01 00:00:00
abstract：:We report a simultaneous study of the VWA STR locus by the Perkin-Elmer Profiler Plus kit and the Promega GenePrint CTTv kit in a population sample from North Portugal and in 418 meiosis from family material and paternity cases. PCR amplification and genotyping were performed according to the manufacturer's instructio...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100215
更新日期：2001-10-01 00:00:00
abstract：:Recently, Verzeletti et al. (Am J Forensic Med Pathol 31:27-33, 2010) developed a new components method for the estimation of age-at-death from the sternal end of the fourth rib. This approach consists of the assignment of numerical scores for several age-related morphological characteristics, which are then inserted ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0735-1
更新日期：2012-09-01 00:00:00
abstract：:Crania are a reliable source for sex estimation in Euro-Americans, Europeans, and most other populations. Besides morphological assessments, the application of Fordisc® has become a useful tool within the last two decades, creating discriminant functions from morphometric data. Unfortunately, until now, white populati...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1469-2
更新日期：2017-07-01 00:00:00
abstract：:Sudden infant death syndrome is the unexpected demise of a child younger than 1 year of age which remains unexplained after a complete autopsy investigation. Usually, it occurs during sleep, in males, and during the first 12 weeks of life. The pathophysiological mechanism underlying the death is unknown, and the letha...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-016-1330-7
更新日期：2016-03-01 00:00:00
abstract：:DNA profiles from degraded samples often suffer from information loss at the longer short tandem repeat (STR) loci. Sensitising the reactions, either by performing additional PCR cycles or increasing the capillary electrophoresis injection settings, carries the risk of over-amplifying or overloading the shorter fragme...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0816-1
更新日期：2013-07-01 00:00:00
abstract：:Forensic statistical parameters based on allelic frequencies of commonly used short tandem repeats were estimated for the Han population of Jiangsu province from P.R. China. The 6-dye GlobalFiler™ PCR amplification kit incorporates 21 autosomal STRs, providing reliable DNA typing results with enhanced the power of dis...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1815-7
更新日期：2018-09-01 00:00:00
abstract：:Five short tandem repeat (STR) systems HumVWA, HumTH01, HumCD4, HumF13B and HumFES were investigated in 2 subpopulations living in Turkey (Laz Turks and Kurds). The population genetic data were compared to a Turkish population sample from the Adana area. A closer genetic relationship was found to the Laz Turks than to...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050059
更新日期：1997-01-01 00:00:00
abstract：:We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up....
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-008-0223-9
更新日期：2008-05-01 00:00:00
abstract：:Diagnosis of ischaemia-related sudden cardiac death in the absence of microscopic and macroscopic ischaemic lesions remains a challenge for medical examiners. Medical imaging techniques increasingly provide support in post-mortem examinations by detecting and documenting internal findings prior to autopsy. Previous st...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02211-0
更新日期：2020-03-01 00:00:00
abstract：:Blood, saliva, and semen are some of the forensically most relevant biological stains commonly found at crime scenes, which can often be of small size or challenging due to advanced decay. In this context, it is of great importance to possess reliable knowledge about the effects of degradation under different environm...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1373-9
更新日期：2016-11-01 00:00:00
abstract：:The utility of postmortem microbiology has continuously been a topic of controversy. The present study describes a case of fatal sepsis in a patient with systemic lupus erythematosus. Postmortem culture and genotyping analyses allowed us to identify Klebsiella pneumoniae as the cause of sepsis, revealing the inadequat...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1160-z
更新日期：2015-09-01 00:00:00
abstract：:In this study, the effect of sample purification on total signal intensities of samples amplified with genRES MPX-2 (nine-locus multiplex) prior to capillary electrophoretic analysis has been investigated. Sample purification with the Qiaquick PCR purification kit led to an increase of the relative fluorescent signal ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-001-0277-4
更新日期：2002-06-01 00:00:00
abstract：:Using AGCU Y24 Plus PCR Amplification Kit, 32 Y short tandem repeat (STR) loci were analyzed in 355 unrelated male participants of Meizhou city in Guangdong Province of China. By analyzing 341 different haplotypes, it was found that haplotype diversity (HD) and discrimination capacity (DC) were 0.9995 and 0.9605, resp...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1845-1
更新日期：2019-03-01 00:00:00
abstract：:The genetic polymorphisms of 21 autosomal short tandem repeat (STR) loci included in the GlobalFiler™ PCR Amplification Kit were evaluated in 3032 unrelated individuals Altay Han of Xinjiang, northwest China. All of the loci reached the Hardy-Weinberg equilibrium. These loci were examined to determine allele frequenci...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1641-3
更新日期：2018-01-01 00:00:00
abstract：:The present study aimed to evaluate the accuracy of mandibular measurements for sex determination in a Brazilian population. The sample was composed of 100 mandibles, of which 53 were female and 47 were male, and the average age was 57.03 years. The mandible measurement protocol was composed of 15 measurements, of whi...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1681-8
更新日期：2018-05-01 00:00:00
abstract：:Shooting incidents are often portrayed as resulting in a sometimes violent backwards displacement of the victim. This opinion is also not infrequently held by expert witnesses. The physical force responsible for this would be momentum (mass x velocity). The physics of momentum in ballistic injury is explained in detai...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/BF01369676
更新日期：1996-01-01 00:00:00
abstract：:Article 25 of the Charter of Fundamental Rights of the European Union (adopted in Nice on 7 December 2000) recognizes and respects the rights of older people to lead a life of dignity and independence and to participate in social and cultural life. It also highlights the importance of prevention and recognition of eld...
journal_title：International journal of legal medicine
pub_type： 杂志文章,实务指引
doi：10.1007/s00414-018-1880-y
更新日期：2019-01-01 00:00:00
abstract：:We immunohistochemically examined the intrathrombotic dynamics of autophagy during thrombogenesis using murine deep vein thrombosis (DVT) models. To perform the immunohistochemical analyses, we used anti-LC3 antibody and anti-p62 antibody for detecting the intrathrombotic autophagic functions. We estimated dynamics of...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02168-0
更新日期：2020-05-01 00:00:00
abstract：:It is difficult to carry out toxicological investigations in biological samples collected from extensively decomposed bodies and to interpret obtained results as several pitfalls should be considered: redistribution phenomena, degradation of xenobiotics during the postmortem period, contamination by putrefaction fluid...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02267-3
更新日期：2020-07-01 00:00:00
abstract：:In the past, numerous studies have documented the possibility of intraindividual differing appearances of the paired age-marker "medial clavicular ossification" in borderline adults. However, the extent of the divergences is rarely mentioned. Against that the article describes this phenomenon from a large sample of 25...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1694-3
更新日期：2018-03-01 00:00:00
abstract：:An accurate and precise documentation of injuries is fundamental in a forensic pathological context. Photographs and manual measurements are taken of all injuries during autopsies, but ordinary photography projects a 3D wound on a 2D space. Using technologies such as photogrammetry, it is possible to create 3D detaile...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1499-9
更新日期：2017-05-01 00:00:00
