当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Cholesterol depletion induces autophagy.

Cholesterol depletion induces autophagy.

Abstract：

:Autophagy is a mechanism to digest cells' own components, and its importance in many physiological and pathological processes is being recognized. But the molecular mechanism that regulates autophagy is not understood in detail. In the present study, we found that cholesterol depletion induces macroautophagy. The cellular cholesterol in human fibroblasts was depleted either acutely using 5mM methyl-beta-cyclodextrin or 10-20microg/ml nystatin for 1h, or metabolically by 20microM mevastatin and 200microM mevalonolactone along with 10% lipoprotein-deficient serum for 2-3 days. By any of these protocols, marked increase of LC3-II was detected by immunoblotting and by immunofluorescence microscopy, and the increase was more extensive than that caused by amino acid starvation, i.e., incubation in Hanks' solution for several hours. The induction of autophagic vacuoles by cholesterol depletion was also observed in other cell types, and the LC3-positive membranes were often seen as long tubules, >50microm in length. The increase of LC3-II by methyl-beta-cyclodextrin was suppressed by phosphatidylinositol 3-kinase inhibitors and was accompanied by dephosphorylation of mammalian target of rapamycin. By electron microscopy, autophagic vacuoles induced by cholesterol depletion were indistinguishable from those seen after amino acid starvation. These results demonstrate that a decrease in cholesterol activates autophagy by a phosphatidylinositol 3-kinase-dependent mechanism.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Cheng J,Ohsaki Y,Tauchi-Sato K,Fujita A,Fujimoto T

doi

10.1016/j.bbrc.2006.10.042

subject

Has Abstract

pub_date

2006-12-08 00:00:00

pages

246-52

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)02265-0

journal_volume

351

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification of small molecule compounds active against Staphylococcus aureus and Proteus mirabilis.

    abstract：:Staphylococcus aureus is a human pathogen rapidly becoming a serious health problem due to ease of acquiring antibiotic resistance. To help identify potential new drug candidates effective against the pathogen, a small focused library was screened for inhibition of bacterial growth against several pathogens, including...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.189

    authors： Swietnicki W,Czarny A,Urbanska N,Drab M

    更新日期：2018-12-02 00:00:00

  • Two domains of the epidermal growth factor receptor are involved in cytoskeletal interactions.

    abstract：:Epidermal growth factor receptor can interact directly with F-actin through an actin-binding domain. In the present study, a mutant EGFR, lacking a previously identified actin-binding domain (ABD 1), was still able to bind elements of the cytoskeleton. A second EGFR actin-binding domain (ABD 2) was identified in the r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.138

    authors： Song W,Wu J,Ge G,Lin Q

    更新日期：2008-06-13 00:00:00

  • Genomic cloning of the human histo-blood group ABO locus.

    abstract：:A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1044

    authors： Bennett EP,Steffensen R,Clausen H,Weghuis DO,van Kessel AG

    更新日期：1995-01-05 00:00:00

  • Regulation of oscillations in filamentous actin content in polymorphonuclear leukocytes stimulated with leukotriene B(4) and platelet-activating factor.

    abstract：:Stimulation of neutrophils with LTB(4) or PAF results in the production of a rapidly oscillating actin polymerization/depolymerization response. Treatment of neutrophils with inhibitors of PKC prior to stimulation with ligand resulted in a masking of the F-actin oscillations. Because myosin has been shown to be a subs...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1222

    authors： Rengan R,Omann GM

    更新日期：1999-08-27 00:00:00

  • The casein kinase 2α promotes the occurrence polycystic ovary syndrome.

    abstract：:Polycystic ovary syndrome (PCOS) is a complicated reproductive endocrine disease characterized by hyperandrogenism, polycystic ovaries, and anovulation. Previous studies have revealed that androgen receptors (ARs) are strongly associated with hyperandrogenism and abnormalities in folliculogenesis in patients with PCOS...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.065

    authors： Yu CJ,Liu X,Zhou ZY,Chen XJ,Meng YC,Gu HC,Xu JJ,Ding GL,Liu XM,Sheng JZ,Huang HF

    更新日期：2020-02-17 00:00:00

  • Immunohistological detection of BRAF25 in human prostate tumor and cancer specimens.

    abstract：:BRAF25 is an alternatively spliced protein of BRAF35 (see associated paper). We have mapped the BRAF25 gene to chromosome sub-band 19p13.3, a region where loss of chromosomal heterozygosity has been reported in about 50% of ovarian cancers. Because of the high incidence of genetic links of prostate cancer to breast an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)00625-3

    authors： Wang C,McCarty IM,Balazs L,Li Y,Steiner MS

    更新日期：2002-07-05 00:00:00

  • Effects of aminoglycoside antibiotics on the coupling of protein and RNA syntheses in Escherichia coli.

    abstract：:The interdependency of protein and RNA syntheses was studied comparatively in bacteria confronted with amino acid starvation or treated separately with various aminoglycoside antibiotics. By contrast with the concomitant inhibition of macromolecular syntheses in cells deprived of an essential amino acid, RNA productio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91688-1

    authors： Cortay JC,Cozzone AJ

    更新日期：1983-05-16 00:00:00

  • X-Linked juvenile retinoschisis associated with a 4-base pair insertion at codon 55 of the XLRS1 gene.

    abstract：:X-linked juvenile retinoschisis (RS) is a bilateral vitreoretinal disorder with no known cure. The gene responsible for the disease was recently isolated by positional cloning methods and a spectrum of mutations has been described in families with RS pathology. In this report, we screened six sporadic cases of RS for ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2133

    authors： Hiraoka M,Trese MT,Shastry BS

    更新日期：2000-02-16 00:00:00

  • MicroRNA-21 promotes osteogenesis of bone marrow mesenchymal stem cells via the Smad7-Smad1/5/8-Runx2 pathway.

    abstract：:Bone marrow mesenchymal stem cells (BMMSCs) are pluripotent stem cells, and the osteogenic differentiation of BMMSCs has been drawing attention for a long time. Bone formation is regulated by numerous molecular and cellular signaling pathways, and the differentiation of BMMSCs is controlled by a well-defined genetic p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.119

    authors： Li X,Guo L,Liu Y,Su Y,Xie Y,Du J,Zhou J,Ding G,Wang H,Bai Y,Liu Y

    更新日期：2017-11-18 00:00:00

  • Induction of E. coli-derived endonuclease MazF suppresses HIV-1 production and causes apoptosis in latently infected cells.

    abstract：:The current antiretroviral therapy cannot cure the patients infected with human immunodeficiency virus type 1 (HIV-1) due to the existence of latently infected cells capable of virus production from harboring proviral DNA. MazF is an ACA nucleotide sequence-specific endoribonuclease derived from Escherichia coli. The ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.07.103

    authors： Okamoto M,Chono H,Hidaka A,Toyama M,Mineno J,Baba M

    更新日期：2020-09-24 00:00:00

  • Engineering of heterologous cytochrome P450 in Acinetobacter sp.: application for pollutant degradation.

    abstract：:Many organisms do not contain the necessary biochemical armoury to carry out the initial oxidative attack of many pollutant chemicals. In the present study, Acinetobacter sp. strain BD413 has been genetically engineered to express the cytochrome P450 xenobiotic-metabolising enzyme CYP105D1 from Streptomyces griseus th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3541

    authors： Lamb DC,Kelly DE,Masaphy S,Jones GL,Kelly SL

    更新日期：2000-09-24 00:00:00

  • A potent and specific agonist, Suc-[Glu9,Ala11,15]-endothelin-1(8-21), IRL 1620, for the ETB receptor.

    abstract：:A series of C-terminal linear peptides of endothelin (ET)-1 and their N alpha-succinyl (Suc) analogs were synthesized and their binding affinities for the two subtypes of ET receptor, ETA and ETB, in porcine lung membranes were examined. Among the synthetic analogs, Suc-[Glu9,Ala11,15]-ET-1(8-21), IRL 1620, was the mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90683-c

    authors： Takai M,Umemura I,Yamasaki K,Watakabe T,Fujitani Y,Oda K,Urade Y,Inui T,Yamamura T,Okada T

    更新日期：1992-04-30 00:00:00

  • Optimization of cofactors which regulate RBL-1 arachidonate 5-lipoxygenase.

    abstract：:The arachidonate lipoxygenase from rat basophilic leukemia cells (RBL-1) is widely utilized as a model to dissect the primary enzymatic reactions leading to leukotriene formation. The purpose of the present study was to optimize the specific activity of 5-lipoxygenase prepared from a high speed supernatant of RBL-1 ce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91388-0

    authors： Cochran FR,Finch-Arietta MB

    更新日期：1989-06-30 00:00:00

  • Reassignment of the human macrophage colony stimulating factor gene to chromosome 1p13-21.

    abstract：:Macrophage colony stimulating factor (CSF-1) is a member of a family of glycoproteins that are necessary for the normal proliferation and differentiation of myeloid progenitor cells. The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91850-p

    authors： Saltman DL,Dolganov GM,Hinton LM,Lovett M

    更新日期：1992-02-14 00:00:00

  • H2S inhibits angiotensin II-induced atrial Kv1.5 upregulation by attenuating Nox4-mediated ROS generation during atrial fibrillation.

    abstract：:Our previous study demonstrated that angiotensin II (Ang II) upregulates the expression of Kv1.5, a promising target for atrial fibrillation (AF) therapy, by activating ROS-dependent P-Smad2/3 and P-ERK 1/2. A recent study showed that hydrogen sulfide (H2S) may modulate the effects of angiotensin II (Ang II) by inhibi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.12.110

    authors： Lu G,Xu C,Tang K,Zhang J,Li Q,Peng L,Wang Y,Huang Z,Gao X

    更新日期：2017-01-29 00:00:00

  • UVA-induced photoaging inhibits autophagic degradation by impairing lysosomal function in dermal fibroblasts.

    abstract：:Autophagy has been associated with a variety of diseases especially aging. Human dermal fibroblasts (HDFs) can internalize and then degrade elastin, collagen and advanced glycation end products (AGEs) in lysosomes, which plays prominent roles in extracellular matrix homeostasis and AGEs removal in the dermis. Although...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.103

    authors： Huang Y,Li Y,Qu Y,Zheng Y,Ouyang M,Zhang Y,Lai W,Xu Q

    更新日期：2019-10-22 00:00:00

  • The integrated stress response and proteotoxicity in cancer therapy.

    abstract：:A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2016.11.047

    authors： McConkey DJ

    更新日期：2017-01-15 00:00:00

  • FGF-23 inhibits renal tubular phosphate transport and is a PHEX substrate.

    abstract：:Oncogenic osteomalacia (OOM), X-linked hypophosphatemia (XLH), and autosomal dominant hypophosphatemic rickets (ADHR) are phenotypically similar disorders characterized by hypophosphatemia, decreased renal phosphate reabsorption, normal or low serum calcitriol concentrations, normal serum concentrations of calcium and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5084

    authors： Bowe AE,Finnegan R,Jan de Beur SM,Cho J,Levine MA,Kumar R,Schiavi SC

    更新日期：2001-06-22 00:00:00

  • 18O labeling on Ser45 but not on Ser35 supports the cooperative phosphorylation mechanism on tarantula thick filament activation.

    abstract：:Thick filaments from some striated muscles are regulated by phosphorylation of myosin regulatory light chains (RLCs). A tarantula thick filament quasi-atomic model achieved by cryo-electron microscopy has advanced our understanding on how this regulation occurs. In native thick filaments, an asymmetric intramolecular ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.01.044

    authors： Sulbarán G,Biasutto A,Méndez F,Pinto A,Alamo L,Padrón R

    更新日期：2020-03-26 00:00:00

  • The characteristic gene expressions of MAPK phosphatases 1 and 2 in hepatocarcinogenesis, rat ascites hepatoma cells, and regenerating rat liver.

    abstract：:mRNA levels of mitogen-activated protein kinase phosphatases, MKP-1 and MKP-2, were determined during chemical hepatocarcinogenesis and during regeneration of rat liver. In chemical hepatocarcinogenesis, the mRNA levels of MKP-1 were increased in primary hepatomas but decreased in rat ascites hepatomas as compared wit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7547

    authors： Yokoyama A,Karasaki H,Urushibara N,Nomoto K,Imai Y,Nakamura K,Mizuno Y,Ogawa K,Kikuchi K

    更新日期：1997-10-29 00:00:00

  • Phospholipase D is associated in a phorbol ester-dependent manner with protein kinase C-alpha and with a 220-kDa protein which is phosphorylated on serine and threonine.

    abstract：:Many studies have shown that phospholipase D (PLD) is activated by protein kinase C (PKC) in vivo and in vitro. In this study, a PLD isoform (rPLD1) was shown to bind to PKC-alpha in Rat1 fibroblasts treated with phorbol ester. The PKC-alpha binding domain of rPLD1 was localized to its N-terminus. The phospholipase wa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8990

    authors： Min DS,Exton JH

    更新日期：1998-07-30 00:00:00

  • Conditional transformation of mouse pancreatic epithelial cells: an in vitro model for analysis of genetic events in pancreatocarcinogenesis.

    abstract：:Pancreatic ductal adenocarcinomas arise through the accumulation of certain genetic alterations including ras, p16, p53, and DPC4. We found that activation of ras and inactivation of p53 could cooperatively induce in vitro tumorigenicity in conditionally immortalized pancreatic epithelial (IMPE) cells. IMPE cells were...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.196

    authors： Koizumi M,Ito D,Fujimoto K,Toyoda E,Kami K,Mori T,Doi R,Whitehead R,Imamura M

    更新日期：2004-06-25 00:00:00

  • The gene, expression pattern and subcellular localization of chitin synthase B from the insect Ostrinia furnacalis.

    abstract：:Insect midgut peritrophic membrane (PM) is a functional structure that protects insects against chemical damage and microorganism infection. The essential component in PM is chitin and its synthesis is catalyzed by Class B chitin synthase (CHSB), which plays a unique role in chitin-containing organisms and thus repres...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.11.111

    authors： Qu M,Liu T,Yang J,Yang Q

    更新日期：2011-01-07 00:00:00

  • Changes of protein kinase C subspecies in staphylococcal enterotoxin-B-induced thymocyte apoptosis.

    abstract：:Our previous studies demonstrated that intravenous administration of staphylococcal enterotoxin B (SEB) to BALB/c mice resulted in thymocyte apoptosis. In the present study, we evaluated the role of protein kinase C (PKC) in thymocyte apoptosis induced by SEB. Our results showed that the level of protein phosphorylati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2244

    authors： Lin YS,Kao SF,Jan MS,Cheng ML,Wing LY,Chang WC,Lei HY,Lin MT

    更新日期：1995-08-24 00:00:00

  • Adherens junction-dependent PI3K/Akt activation induces resistance to genotoxin-induced cell death in differentiated intestinal epithelial cells.

    abstract：:The crypt-villi axis of intestinal mucosa maintains homeostasis by renewal of epithelia, and also exhibits different properties from undifferentiated to terminally differentiated cells. We investigated differential susceptibility to genotoxin-induced cell death, based on the degree of differentiation of epithelial cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.120

    authors： Chae B,Yang KM,Kim TI,Kim WH

    更新日期：2009-01-23 00:00:00

  • Alteration of phosphoinositide metabolism by attenuation of cAMP resulting from expression of the H-ras oncogene.

    abstract：:Most studies characterizing H-ras have been conducted in constitutively expressing cell lines. To explore the early interaction between H-ras p21 and signal transduction systems we have utilized an NIH3T3 fibroblast line transfected with a steroid inducible MMTV H-ras vector. Exposure to dexamethasone resulted in tran...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2782

    authors： Murphy SK,Lindberg JB,Prussack DA

    更新日期：1994-12-15 00:00:00

  • Structure of acrosome reaction-inducing substance in the jelly coat of starfish eggs: a mini review.

    abstract：:Our knowledge at present on the structure of acrosome-reaction inducing substance (ARIS) in the jelly coat of starfish eggs is summarized. ARIS ia a proteoglycan-like molecule consisting of very long, linear, and highly sulfated glycans and three ARIS proteins, ARIS1-3. Detailed structures of the major glycan of ARIS ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2012.08.033

    authors： Hoshi M,Moriyama H,Matsumoto M

    更新日期：2012-08-31 00:00:00

  • Stoichiometric interactions between cyanobacterial clock proteins KaiA and KaiC.

    abstract：:We determined the stoichiometry of KaiA-KaiC interactions. Using immunoblotting and two-dimensional Native- and SDS-PAGE (2DNS-PAGE) analysis, we demonstrated that the reaction products of KaiA-KaiC interactions in the presence of ATP consisted of only phosphorylated KaiC whereas in the presence of the unhydrolyzable ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.02.034

    authors： Hayashi F,Ito H,Fujita M,Iwase R,Uzumaki T,Ishiura M

    更新日期：2004-03-26 00:00:00

  • Mass spectrometric identification of peptides present in immunized and parasitised hemolymph from honeybees without purification.

    abstract：:We propose that a substance, identified using mass spectrometry, present in the hemolymph of the honeybee (Apis mellifera) as a result of stimulating the insect immune system corresponds with Apidaecin I, an antibacterial peptide recently described. The plasma desorption mass spectra indicate that several other higher...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80013-0

    authors： Craig AG,Trenczek T,Fries I,Bennich H

    更新日期：1989-12-15 00:00:00

  • Identification of a novel Ca2+-stimulated S6-kinase in rat liver.

    abstract：:Extracellular calcium addition transiently stimulated two S6 peptide kinase activities in isolated rat hepatocytes. Mono Q chromatography revealed that the activities eluting at 0.15 M NaCl and 0.18 M NaCl were stimulated 4-fold and 2-fold, respectively. The kinase stimulated by calcium was a 40000-Mr S6 peptide kinas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7175

    authors： Harlin JC,Tredano M,Rider MH,Bollen M,Chédeville A,Lavoinne A

    更新日期：1997-10-20 00:00:00