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Engineering of heterologous cytochrome P450 in Acinetobacter sp.: application for pollutant degradation.

Abstract：

:Many organisms do not contain the necessary biochemical armoury to carry out the initial oxidative attack of many pollutant chemicals. In the present study, Acinetobacter sp. strain BD413 has been genetically engineered to express the cytochrome P450 xenobiotic-metabolising enzyme CYP105D1 from Streptomyces griseus that has in its repertoire a diverse array of organic pollutants. Further, it is shown that the transformed Acinetobacter calcoaceticus strain BD413 can grow on pollutants unlike control bacteria not expressing cytochrome P450 and that was reflected in release of radiolabel with growth on radiolabelled chlortoluron. We show that cytochrome P450 can enhance the biodegrading repertoire of A. calcoaceticus and discuss the application of such results to bioremediation strategies.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lamb DC,Kelly DE,Masaphy S,Jones GL,Kelly SL

doi

10.1006/bbrc.2000.3541

subject

Has Abstract

pub_date

2000-09-24 00:00:00

pages

797-802

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(00)93541-1

journal_volume

276

pub_type

杂志文章

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