Balancing of energy-consuming processes of rat hepatocytes.

Abstract:

:A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy-requiring reactions, the energy demands of protein turnover, nucleic acid synthesis, Na+/K(+)-ATPase and Ca2(+)-transport of hepatocytes in different incubation media were assessed. These processes together with urea synthesis account for about 60 per cent of the total energy consumption in a glucose and amino acid-enriched Eagle/Borsook medium. The metabolic flux rates of total ATP-consumption and ATP-consumption of single energy-requiring processes in hepatocytes are compared with those in reticulocytes and different tumour cell types.

journal_name

Cell Biochem Funct

authors

Schneider W,Siems W,Grune T

doi

10.1002/cbf.290080407

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

227-32

issue

4

eissn

0263-6484

issn

1099-0844

journal_volume

8

pub_type

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