Abstract:
:A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy-requiring reactions, the energy demands of protein turnover, nucleic acid synthesis, Na+/K(+)-ATPase and Ca2(+)-transport of hepatocytes in different incubation media were assessed. These processes together with urea synthesis account for about 60 per cent of the total energy consumption in a glucose and amino acid-enriched Eagle/Borsook medium. The metabolic flux rates of total ATP-consumption and ATP-consumption of single energy-requiring processes in hepatocytes are compared with those in reticulocytes and different tumour cell types.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Schneider W,Siems W,Grune Tdoi
10.1002/cbf.290080407subject
Has Abstractpub_date
1990-10-01 00:00:00pages
227-32issue
4eissn
0263-6484issn
1099-0844journal_volume
8pub_type
杂志文章abstract::Corneal scarring is the result of a disease, infection or injury. The resulting scars cause significant loss of vision or even blindness. To-date, the most successful treatment is corneal transplantation, but it does not come without side effects. One of the corneal dystrophies that are correlated with corneal scarrin...
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