Abstract:
:The aim of this study was to investigate the possibility of enhancing the yield of tissue plasminogen activator (tPA) from two epithelial cell lines of normal (non-malignant) derivation grown in tissue culture. The three agents used in this investigation were chosen because of their proven enhancing effect on analogous cells or products. The anabolic hormone stanozolol was found to have no significant stimulatory effect on these cell lines. A phorbol acetate (12-O-tetradecanoylphorbol 13-acetate) caused a twofold enhancement in tPA yield but the most significant results were obtained with 5-azacytidine. This agent increased the yield by up to fourfold in small stationary cultures and threefold in large-scale microcarrier cultures. A combination of azacytidine and phorbol acetate did not have an additive effect on total yield but did alter the kinetics of tPA expression with time. Indications were that the maximum yield with these types of potentiating agents was achieved as it could not be increased by using a combination of two different agents.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Electricwala A,Griffiths Bdoi
10.1002/cbf.290040109subject
Has Abstractpub_date
1986-01-01 00:00:00pages
55-60issue
1eissn
0263-6484issn
1099-0844journal_volume
4pub_type
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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pub_type: 杂志文章,评审
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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