Abstract:
:The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylglycerol (DAG)-, phosphatidylinositol (PI)- and phosphatidylinositol-4-phosphate (PIP)-kinase activity, with differences between total tissue homogenates and isolated nuclei, relevant to the treatment in vivo. Administration of hydrocortisone (HC) produced a marked decrease in the phosphorylated nuclear products without influencing the homogenate kinase activity. Under conditions which were optimal for the kinase activities, nuclear PIP-kinase was strongly increased in presence of a high blood level of HC whereas PI-kinase activity was reduced. From these observations it appears that the observed differences were due to specific modulation of kinase activities rather than to changes in the availability of substrates. The phosphoinositide-specific phospholipase C (PLC) activity was also investigated. In the presence of a high HC blood level, the phosphodiesteratic cleavage of PIP strongly increased, while that of phosphatidylinositol bisphosphate (PIP2) was similar in normal and hypercortisolemic conditions. Nuclear phosphoinositide hydrolysis was affected by PLC, beta and gamma isoforms, which were equally represented in all the conditions investigated, indicating that the observed changes of activity were due to a modulation rather than to a change in the amount of enzyme. These results suggest that inositol lipid metabolism plays a role in the nuclear modifications accompanying steroid hormone induction of transcriptional activity.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Santi P,Marmiroli S,Falcieri E,Bertagnolo V,Capitani Sdoi
10.1002/cbf.290120308subject
Has Abstractpub_date
1994-09-01 00:00:00pages
201-7issue
3eissn
0263-6484issn
1099-0844journal_volume
12pub_type
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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doi:10.1002/cbf.1286
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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journal_title:Cell biochemistry and function
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abstract::The aim of this experimental study was to investigate the possible role of adenosine deaminase (AD) and xanthine oxidase (XO) in the pathogenesis of cisplatin-induced nephrotoxicity and the effect of erdosteine in decreasing the toxicity. The intraperitoneal injection of cisplatin (7 mg kg(-1) body weight) induced a s...
journal_title:Cell biochemistry and function
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