Abstract:
:Melanin plays an important role in determining skin colour. Apoptosis of melanocytes and defect in melanin production cause vitiligo. Various studies have been conducted to treat the disease, but its treatment is still difficult. Therefore, this study aimed to investigate the effect of spermidine, which is known as an inhibitor of ageing-related oxidized proteins, on melanogenesis. Even though spermidine above 50 μM had no effect on antioxidant activity and DOPA oxidation, it displayed tyrosinase activity. However, spermidine at 2000 μM was cytotoxic in B16F1 cells using MTT assay. Spermidine above 125 μM decreased the amount of intracellular hydrogen peroxide in a concentration-dependent manner in DCFH-DA analysis. It was also found that spermidine above 2000 μM increased melanin synthesis in living cells. However, spermidine above 1000 μM increased melanin synthesis in a concentration-dependent manner in H2 O2 -treated B16F1 cells. Furthermore, spermidine enhanced the expression of tyrosine hydroxylase via MITF transcription factor involved in melanogenesis in H2 O2 -treated B16F1 cells. Therefore, these results suggest that spermidine could be applied as a potential stimulator of melanin synthesis for the prevention of hair greying.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Kang SH,Kim MMdoi
10.1002/cbf.3619subject
Has Abstractpub_date
2021-01-25 00:00:00eissn
0263-6484issn
1099-0844pub_type
杂志文章abstract::Cardiovascular diseases are known to be the most fatal diseases worldwide. Ischaemia/reperfusion (I/R) injury is at the centre of the pathology of the most common cardiovascular diseases. According to the World Health Organization estimates, ischaemic heart disease is the leading global cause of death, causing more th...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3587
更新日期:2020-09-06 00:00:00
abstract::A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080407
更新日期:1990-10-01 00:00:00
abstract::The present investigation focused, firstly, on the effects of oral administration of thymoquinone (TQ) on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase activity in hepatic, cardiac and kidney tissues of normal mice. Superoxide dismutase (SOD; E.C:1.15.1.1), catalase (CAT; E.C:1.11.1.6), glutathio...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.968
更新日期:2002-06-01 00:00:00
abstract::The protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Animals were divided into three groups: control, ethanol and ethanol + betaine group. Animals were fed liquid diets and consumed approximately 60 diet per day. Rats were fed ethanol 8 kg(- 1) day(- 1). The et...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.926
更新日期:2001-12-01 00:00:00
abstract::The spreading of HeLa cells on plastic substratum is mediated by fibronectin-depleted foetal calf serum but not by fibronectin isolated by gelatin-Sepharose affinity chromatography. The same is true for freshly explanted chick embryonic chondrocytes. In contrast, BHK cell spreading exceeds 67% after 120 min at 37 degr...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290010208
更新日期:1983-07-01 00:00:00
abstract::Proliferating-cell nuclear antigen (PCNA), also known as cyclin, is synthesized in proliferative cells and recently was identified as DNA polymerase-delta auxiliary protein. In this paper, the association of PCNA to the proliferative cells of plants was analysed using both autoantibodies to PCNA obtained from a patien...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100209
更新日期:1992-06-01 00:00:00
abstract::Human cytochrome P450 2J2 (CYP2J2) is abundant in cardiovascular tissue and active in the metabolism of arachidonic acid to eicosanoids that have potent vasodilatory properties. Variability of the CYP2J2 gene is highly constrained except for its proximal promoter: there is a relatively common and functionally relevant...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1512
更新日期:2008-10-01 00:00:00
abstract::Diabetes induces oxidative stress in aged human and rat, although daily supplementation of vitamins C and E (VCE) can be beneficial to aged diabetic rats by reducing free radical production. The aim of the present study was to evaluate whether dietary VCE supplementation relieves oxidative stress in streptozotocin (ST...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1749
更新日期:2011-06-01 00:00:00
abstract::Bone tumor incidence in women peaks at age 50-60, coinciding with the menopause. That estrogen (E2) and triiodothyronine (T3) interact in bone metabolism has been well established. However, few data on the action of these hormones are available. Our purpose was to determine the role of E2 and T3 in the expression of b...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1408
更新日期:2008-01-01 00:00:00
abstract::The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second deh...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290090207
更新日期:1991-04-01 00:00:00
abstract::Analysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides. 3H-adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non-radioactive a...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020310
更新日期:1984-07-01 00:00:00
abstract::We investigated the effect of rosiglitazone (RSG), a high-affinity ligand for the peroxisome proliferator-activated receptor gamma which mediates insulin-sensitizing actions, on the lipid profile and oxidative status in streptozotocin (STZ)-induced Type 2 diabetes mellitus (DM) rats. Wistar albino male rats were rando...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1469
更新日期:2008-06-01 00:00:00
abstract::Nuclear T3 specific binding sites were characterized by Scatchard analyses of L-125I-T3 binding to nuclei extracted from freshly isolated and 1, 2 and 6 day-cultured hepatocytes. The results demonstrate a marked decrease in T3 binding capacity of nuclei extracted from 1 day-cultured cells followed by an almost complet...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030203
更新日期:1985-04-01 00:00:00
abstract::In response to concanavalin A, cytoplasmic calcium movement was observed in human platelets, both in the presence of 1 mM Ca2+ or 1 mM EGTA in the medium. Concanavalin A also caused the activation of inositide turnover and the production of inositol phosphates, suggesting that activation of phospholipase C occurs. The...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100109
更新日期:1992-03-01 00:00:00
abstract::Mesenchymal epithelial transition (C-MET) factor overexpression has been found in many types of cancer and has served as an important molecular target for therapeutic intervention. However, the role of C-MET in retinoblastoma remains largely unclear. The present study aimed to investigate the potential role and mechan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3464
更新日期:2020-03-01 00:00:00
abstract::Voltage-gated Ca(2+) channels regulate cardiac automaticity, rhythmicity and excitation-contraction coupling. Whereas L-type (Cav 1·2, Cav 1·3) and T-type (Cav 3·1, Cav 3·2) channels are widely accepted for their functional relevance in the heart, the role of Cav 2·3 Ca(2+) channels expressing R-type currents remains ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2918
更新日期:2013-07-01 00:00:00
abstract::This study was designed to investigate the role of HO-1 induction in prevention of thioacetamide (TAA)-induced oxidative stress, inflammation and liver damage. The changes in hepatic dimethylarginine dimethylaminohydrolase (DDAH) activity as well as plasma arginine and asymmetric dimethylarginine (ADMA) levels were al...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2866
更新日期:2013-03-01 00:00:00
abstract::The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylgl...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120308
更新日期:1994-09-01 00:00:00
abstract::Several studies have highlighted the potential of leucine supplementation for the treatment of metabolic diseases including type 2 diabetes and obesity. Caloric restriction is a common approach to improve the health in diabetic and obese subjects. However, very few studies assessed the effects of leucine supplementati...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3017
更新日期:2014-06-01 00:00:00
abstract::UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several s...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1021
更新日期:2003-09-01 00:00:00
abstract::This study evaluated the effect of possible synergic interaction between high fat diet (HF) and hydrochlorothiazide (HCTZ) on biochemical parameters of oxidative stress in brain. Rats were fed for 16 weeks with a control diet or with an HF, both supplemented with different doses of HCTZ (0.4, 1.0, and 4.0 g kg(-1) of ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1599
更新日期:2009-10-01 00:00:00
abstract::Systemic sclerosis (SSc) is an autoimmune disease characterized by inflammation and fibrosis of the skin and visceral organs. Fibrosis associated with SSc is characterized by an increased synthesis of a wide range of extracellular matrix (ECM). TGF-beta is a pluripotent cytokine in a wide range of cell types. In parti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1649
更新日期:2010-06-01 00:00:00
abstract::Ras-GTPases regulate many central signalling pathways in the cell. Hypoxia induces nitrosative/oxidative stress and dysregulates Ras-dependent downstream processes. H-Ras possesses two cysteine residues (C181 and C184) in the C-termini, which are palmitoylated once or twice. Palmitoylation is sufficient for promoting ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3431
更新日期:2019-10-01 00:00:00
abstract::Current studies have shown that circular RNAs (circRNAs) and microRNAs (miRNAs) are closely related in acute myocardial infarction (AMI). Previous studies have shown that miR-125b promotes fibrosis and up-regulates in cardiac fibroblasts (CFs), and further experiments showed that circ_LAS1L had multiple binding sites ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3486
更新日期:2020-06-01 00:00:00
abstract::Deficiency in neutrophils (neutropenia) caused by mutations in neutrophil elastase (NE, ELA2) has been extensively investigated. Monocytes and neutrophils are derived from a common myeloid progenitor; therefore, ELA2 mutations can also influence monocyte development. These effects have not been well described. In this...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2900
更新日期:2013-06-01 00:00:00
abstract::It is generally accepted that hypertension and other vascular pathologies increase in diabetes mellitus (DM) patients as a result of the renin-angiotensin-aldosterone (RAA) system. In this study, changes in the renin-angiotensin-aldosterone (RAA) system level was determined in Streptozotocin (STZ)-injected rats. A tot...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199909)17:3<193::AID-CBF82
更新日期:1999-09-01 00:00:00
abstract::Sonic hedgehog (Shh) plays important roles in developmental of vertebrate animal central nervous system (CNS), and Gli is its downstream signal molecule. Shh signalling is essential for pattern formation, cell-fate specification, axon guidance, proliferation, survival and differentiation of neurons in CNS development....
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3582
更新日期:2020-08-25 00:00:00
abstract::The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in h...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1606
更新日期:2009-12-01 00:00:00
abstract::Glioblastoma is one of the most malignant tumors and causes the high mortality in cancer patients. Currently, there is no highly efficient therapy against glioblastoma. Therefore, searching for a new molecular target to anti-glioblastoma therapy is urgent and necessary. In this study, we elucidated the role of Signal ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3518
更新日期:2020-07-01 00:00:00
abstract::Lipopolysaccharide (LPS)-induced inflammatory factors production by the cerebral cortical glial cells in two sides of the murine brain are different. To determine if microglial cells, a subset of glial cells, are involved in asymmetric production, interleukin-6 (IL-6), interleukin-1β (IL-1β) and nitric oxide (NO) resp...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1718
更新日期:2011-01-01 00:00:00