Abstract:
:The subcellular distribution of newly absorbed iron in isolated mouse duodenal enterocytes was investigated by analytical subcellular fractionation using sucrose density gradient centifugation. Two major peaks of mucosal 59Fe activity were observed: one soluble and one particulate (density 1.18-1.20 g ml-1). The latter was increased following prior exposure of animals to chronic hypoxia. The particulate 59Fe was localized to the basolateral membranes using the marker enzyme Na+, K+ activated, Mg2+ dependent, ATPase and by washing intact enterocytes with the selective plasma membrane perturbant digitonin. The basolateral membrane can be selectively labelled by in vitro incubation of intact enterocytes at 0 degrees C with 59Fe(III)-nitrilotriacetate complex, confirming the presence of a 59Fe binding site on this membrane. No significant difference in in vitro iron binding to this site was observed between normal and chronically hypoxic animals. Iron binding to the basolateral membrane was significantly higher in disrupted, compared to intact enterocytes, indicating that this site is present on both sides of the basolateral membrane. It is therefore suggested that the increased labelling of this site in hypoxia, in vivo, is a consequence of an increase in a mucosal Fe pool which is available for binding to a membrane receptor.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Snape S,Simpson RJ,Peters TJdoi
10.1002/cbf.290080205subject
Has Abstractpub_date
1990-04-01 00:00:00pages
107-15issue
2eissn
0263-6484issn
1099-0844journal_volume
8pub_type
杂志文章abstract::Vascular endothelial growth factor C (VEGF-C) is an angiogenic and lymphangiogenic growth factor. Recent research has revealed the role for VEGF-C in regulating autophagy by interacting with a nontyrosine kinase receptor, neuropilin-2 (NRP-2). However, whether VEGF-C participates in regulating cell survival and autoph...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3402
更新日期:2019-06-01 00:00:00
abstract::A previous study showing that ethanol (ETOH) blocked [3H]choline incorporation into phosphatidylcholine (PC) suggested an inhibition of PC biosynthesis in human leukemic monocyte-like U937 cells. The mechanism of the inhibitory action of ETOH was investigated. Cells were pulsed with [3H]choline for 30 min and chased i...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120107
更新日期:1994-01-01 00:00:00
abstract::Development and homeostasis of multicellular organisms require interactions between neighbouring cells. We recently established an in vitro model of cell-cell interaction based on a collagen vitrigel membrane. We have now examined the role of neural cells in retinal homeostasis by coculture of human retinal pigment ep...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3082
更新日期:2015-01-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::To determine the temporal roles of phosphatidylinositol 3-kinase (PI3-kinase) and phospholipase D (PLD) during human neutrophil activation stimulated by a chemotactic peptide, we examined the kinetics of these enzymes and related them to a neutrophil function (superoxide production). Both wortmannin and 2-(4-morpholin...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.898
更新日期:2001-03-01 00:00:00
abstract::The object was to determine the influence of dietary vitamin E, selenium and their combination on the fatty acid content of erythrocytes, bone marrow and spleen lipids of Akkaraman lambs. After supplementation for 15 days, the amount of all fatty acids was slightly higher (p < 0.05) in the vitamin E as compared to the...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199703)15:1<1::AID-CBF694>
更新日期:1997-03-01 00:00:00
abstract::The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF7
更新日期:1997-06-01 00:00:00
abstract::Astroglial cells dispersed from newborn rat hemispheres were established in medium supplemented with 20 per cent fetal calf serum (FBS) and then grown to a confluent monolayer in the presence of 10 per cent FBS or charcoal-stripped FBS (CS). Type 1 astrocytes were subcultured and either maintained under the same condi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.645
更新日期:1996-03-01 00:00:00
abstract::Cardiac hypertrophy is characterized by remodeling of the extracellular matrix (ECM). Integrins are cell-surface molecules that link the ECM to the cellular cytoskeleton where they play roles as signaling molecules and transducers of mechanical force. To clarify the possible roles of integrins in cardiac myocyte hyper...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.988
更新日期:2003-03-01 00:00:00
abstract::Gout is an inflammatory joint disorder characterized by hyperuricaemia and precipitation of monosodium urate crystals in the joints. In the present study, we aimed to investigate the anti-inflammatory effect of trikatu, a herbal compound in monosodium urate crystal-induced inflammation in rats, an experimental model f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2979
更新日期:2014-01-01 00:00:00
abstract::Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 +/- 7.7 mU/10(6) cells (mean +/- S.E.) versus 440 +/- 62 mU/10(6) cells for the hyd...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290050304
更新日期:1987-07-01 00:00:00
abstract::Because of limited treatment options, preeclampsia (PE) is the leading cause of perinatal morbidity and mortality worldwide. Recently, lncRNA TDRG1 is reported to be aberrantly down-regulated in PE placenta, and the abnormal expression of TDRG1 might play a key or partial role in PE development. In this study, we foun...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3480
更新日期:2020-06-01 00:00:00
abstract::Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer, and thymidine phosphorylase (TP) is a regulator of angiogenesis. To investigate the biological activities of TP in ICC, we established human cholangiocarcinoma RBE cell lines overexpressing TP or silencing TP. Overexpression of TP en...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3541
更新日期:2020-08-01 00:00:00
abstract::The possibility that inositol lipid metabolism is related to nuclear events accompanying steroid hormone action has been investigated by comparing lipid phosphorylation and breakdown in normal rat liver nuclei and in hypo- and hypercortisolemic conditions. Lipid phosphorylation in vitro showed the presence of diacylgl...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120308
更新日期:1994-09-01 00:00:00
abstract::Mitochondrial, endoplasmic reticular and plasma membrane fractions were isolated by a new method from control male Fischer 344 rats and rats given CCl4 by gavage. After 1 h of CCl4 treatment, rats were in glucose and pancreatic hormone balance but plasma levels of T3 and T4 were decreased 29 and 22%, respectively. Aft...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.934
更新日期:2002-03-01 00:00:00
abstract::The influence of the 1,4-dihydropyridines (DHPs), water-soluble glutapyrone available as sodium, potassium and ammonium salts of 2-(2,6-dimethyl-3,5-diethoxycarbonyl-1,4-DHP-4-carboxamide)glutaric acid, from one side, and a lipophylic cerebrocrast, 2-propoxyethyl 2,6-dimethyl-4-(2-difluoromethoxyphenyl)-1,4-DHP-3,5-di...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199709)15:3<211::AID-CBF74
更新日期:1997-09-01 00:00:00
abstract::Aberrant innate and adaptive immune responsed to allergens and environmental pollutants lead to respiratory allergic disease such as asthma. In this study, we focused on toll-like receptor-4 (TLR4) expressed on airway epithelium to identify house dust mite (HDM)-regulated allergic inflammation via TLR4 signaling pathw...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1697
更新日期:2010-10-01 00:00:00
abstract::The activity and hormonal regulation of NAD- and NADP-linked isocitrate dehydrogenase (EC.1.1.1.41 and EC.1.1.1.42, respectively) in the brain, liver and kidney cortex of female rats of various ages was investigated. The activity of NAD-ICDH of brain was greater than extramitochondrial (-c) or intramitochondrial (-m) ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290060308
更新日期:1988-07-01 00:00:00
abstract::We constructed the significant low-expression P-glycoprotein (ABCB1) inhibited transport and signal network in chimpanzee compared with high-expression (fold change ≥2) the human left cerebrum in GEO data set, by using integration of gene regulatory activated and inhibited network inference method with gene ontology (...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2837
更新日期:2012-10-01 00:00:00
abstract::Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed. In this report, we investigate S...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2976
更新日期:2014-01-01 00:00:00
abstract::The aim of this work was to determine the role of intraperitoneally-administered vitamin E and selenium on the biochemical and haematological parameters in the blood of rats. Thirty-two adult male Wistar rats were used in this study. All rats were randomly divided into four groups. The first group was used as the cont...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199906)17:2<143::AID-CBF80
更新日期:1999-06-01 00:00:00
abstract::Glucocorticoids are important for skeletal muscle energy metabolism, regulating glucose utilization, insulin sensitivity, and muscle mass. Nicotinamide adenine dinucleotide phosphate-dependent 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1)-mediated glucocorticoid activation in the sarcoplasmic reticulum (SR) is in...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3272
更新日期:2017-07-01 00:00:00
abstract::The purpose of this study was to investigate the oxidative status in experimental hypothyroidism and the antioxidant effect of taurine supplementation. Forty male Sprague Dawley rats were randomly divided into four groups (group 1, control; group 2, control + taurine; group 3, propylthiouracil (PTU); group 4, PTU + ta...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1198
更新日期:2006-03-01 00:00:00
abstract::The aim of the present studies was to determine whether the mechanism of biological action of garlic-derived sulfur compounds in human hepatoma (HepG2) cells can be dependent on the presence of labile sulfane sulfur in their molecules. We investigated the effect of allyl sulfides from garlic: monosulfide, disulfide an...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1835
更新日期:2012-04-01 00:00:00
abstract::This study was designed to investigate the role of HO-1 induction in prevention of thioacetamide (TAA)-induced oxidative stress, inflammation and liver damage. The changes in hepatic dimethylarginine dimethylaminohydrolase (DDAH) activity as well as plasma arginine and asymmetric dimethylarginine (ADMA) levels were al...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2866
更新日期:2013-03-01 00:00:00
abstract::The MCF-7 human breast carcinoma cell line is widely used as a model system by breast cancer researchers and cell biologists investigating apoptosis. Since its establishment 30 years ago, from a patient with metastatic breast cancer, the original MCF-7 cell population has undergone genetic drift to such an extent that...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1106
更新日期:2004-09-01 00:00:00
abstract::Reactive oxygen metabolites play an important role in ischemia-reperfusion related gastric injury. Primary sources of reactive oxygen metabolites seem to be the xanthine/xanthine oxidase system and neutrophils accumulating within the reperfused tissue. Tissue myeloperoxidase activity is an important index of neutrophi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1251
更新日期:2006-09-01 00:00:00
abstract::Lipopolysaccharide (LPS)-induced inflammatory factors production by the cerebral cortical glial cells in two sides of the murine brain are different. To determine if microglial cells, a subset of glial cells, are involved in asymmetric production, interleukin-6 (IL-6), interleukin-1β (IL-1β) and nitric oxide (NO) resp...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1718
更新日期:2011-01-01 00:00:00
abstract::Glioblastoma is one of the most malignant tumors and causes the high mortality in cancer patients. Currently, there is no highly efficient therapy against glioblastoma. Therefore, searching for a new molecular target to anti-glioblastoma therapy is urgent and necessary. In this study, we elucidated the role of Signal ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3518
更新日期:2020-07-01 00:00:00
abstract::Melanin plays an important role in determining skin colour. Apoptosis of melanocytes and defect in melanin production cause vitiligo. Various studies have been conducted to treat the disease, but its treatment is still difficult. Therefore, this study aimed to investigate the effect of spermidine, which is known as an...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3619
更新日期:2021-01-25 00:00:00