Abstract:
:To determine the temporal roles of phosphatidylinositol 3-kinase (PI3-kinase) and phospholipase D (PLD) during human neutrophil activation stimulated by a chemotactic peptide, we examined the kinetics of these enzymes and related them to a neutrophil function (superoxide production). Both wortmannin and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY294002), potent and specific inhibitors of PI3-kinase, inhibit PI3-kinase activity in human neutrophils and significantly inhibit superoxide production from the early phase. Ethanol has no effect on PI3-kinase and markedly inhibits superoxide production at the late phase. Although these agents are inhibitory to different degrees, when neutrophils are simultaneously treated with ethanol and PI3-kinase inhibitors, superoxide is not produced. These results suggest that PI3-kinase and PLD play a pivotal role in the signal transduction pathway of the chemo-attractant-receptor involved neutrophil activation. These enzymes produce second messengers which are required for subsequent superoxide production in human neutrophils. NADPH oxidase is activated in a PI3-kinase-dependent manner at the early phase, and PLD activity follows it and is related to superoxide production at the late phase in human neutrophils by stimulation with FMLP.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Yasui K,Komiyama Adoi
10.1002/cbf.898subject
Has Abstractpub_date
2001-03-01 00:00:00pages
43-50issue
1eissn
0263-6484issn
1099-0844pii
10.1002/cbf.898journal_volume
19pub_type
杂志文章abstract::Incidence and mortality rates of cancer continue to increase greatly despite the improved diagnostic and therapeutic methods. Based on GLOBOCAN estimates, the numbers of new cancer cases reported in 2018 were ~18.1 million, while the numbers of cancer mortalities were ~9.6 million. It remains difficult to diagnose mos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3601
更新日期:2020-11-06 00:00:00
abstract::Involvement of complications is considered to be one of the major factors in the prognosis of diabetes mellitus (DM). Recent studies indicate that most diabetic complications such as nephropathy and hypertension are vascular-originated. Renin-angiotensin involvement, especially changes in ACE activity level, is consid...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(200001/03)18:1<23::AID-CBF
更新日期:2000-03-01 00:00:00
abstract::Voltage-gated Ca(2+) channels regulate cardiac automaticity, rhythmicity and excitation-contraction coupling. Whereas L-type (Cav 1·2, Cav 1·3) and T-type (Cav 3·1, Cav 3·2) channels are widely accepted for their functional relevance in the heart, the role of Cav 2·3 Ca(2+) channels expressing R-type currents remains ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2918
更新日期:2013-07-01 00:00:00
abstract::Bisphenol A (BPA) can be accumulated into the human body via food intake and inhalation. Numerous studies indicated that BPA can trigger the tumorigenesis and progression of cancer cells. Laryngeal cancer cells can be exposed to BPA directly via food digestion, while there were very limited data concerning the effect ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3265
更新日期:2017-06-01 00:00:00
abstract::Specific binding of a fully biologically active 125I-gonadotrophin releasing hormone (GnRH) to isolated anterior pituitary cells is time dependent, saturable and the concentration dependent binding curves exhibit positive cooperativity. Binding to intact or solubilized plasma membranes and an affinity purified GnRH re...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020405
更新日期:1984-10-01 00:00:00
abstract::The heart responds to an increased demand arising due to physiological stimuli or pathological insults by hypertrophy of myocytes. Reactive oxygen species (ROS) have recently been identified as the molecular intermediates in the translation of mechanical stimuli to cellular response. Different signal transduction path...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1449
更新日期:2008-04-01 00:00:00
abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signalling pathways including cervical cancer. However, the underlying mechanisms are still not well elucidated. Here, we assessed the effect of silibinin...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1842
更新日期:2012-04-01 00:00:00
abstract::The acute effects of isolated and combined L-alanine (L-Ala) and L-glutamine (L-Gln) on liver gluconeogenesis, ureagenesis and glycaemic recovery during short-term insulin-induced hypoglycaemia (IIH) were investigated. For this purpose, 24-h fasted rats that received intraperitoneal injection of regular insulin (1.0 U...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1319
更新日期:2007-03-01 00:00:00
abstract::Cardiac hypertrophy is characterized by remodeling of the extracellular matrix (ECM). Integrins are cell-surface molecules that link the ECM to the cellular cytoskeleton where they play roles as signaling molecules and transducers of mechanical force. To clarify the possible roles of integrins in cardiac myocyte hyper...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.988
更新日期:2003-03-01 00:00:00
abstract::Mitochondrial proton leak can account for almost 20% of oxygen consumption and it is generally accepted that this process contributes to basal metabolism. In order to clarify the role of basal proton leak in testicular mitochondria, we performed a comparative study with kidney and liver mitochondrial fractions. Proton...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1644
更新日期:2010-04-01 00:00:00
abstract::The subcellular distribution of newly absorbed iron in isolated mouse duodenal enterocytes was investigated by analytical subcellular fractionation using sucrose density gradient centifugation. Two major peaks of mucosal 59Fe activity were observed: one soluble and one particulate (density 1.18-1.20 g ml-1). The latte...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080205
更新日期:1990-04-01 00:00:00
abstract::The consumption of protein supplements containing amino acids is increasing around the world. Aspartate (Asp) and asparagine (Asn) are amino acids metabolized by skeletal muscle. This metabolism involves biochemical pathways that are involved in increasing Krebs cycle activity via anaplerotic reactions, resulting in h...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1606
更新日期:2009-12-01 00:00:00
abstract::Analysis of free nucleotide composition of both avian thrombocytes and pig platelets showed quantitative differences in the level of adenine nucleotides. 3H-adenine taken up by turkey thrombocytes was metabolized mainly to adenine nucleotides was not released after thrombin action. Thrombin liberated non-radioactive a...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020310
更新日期:1984-07-01 00:00:00
abstract::Proliferating-cell nuclear antigen (PCNA), also known as cyclin, is synthesized in proliferative cells and recently was identified as DNA polymerase-delta auxiliary protein. In this paper, the association of PCNA to the proliferative cells of plants was analysed using both autoantibodies to PCNA obtained from a patien...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100209
更新日期:1992-06-01 00:00:00
abstract::The effects of either radiation or hyperthermia on the differentiation potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108-15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action poten...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120209
更新日期:1994-06-01 00:00:00
abstract::Endometrial cancer (EC) is the most common gynaecologic malignancy in western countries and has been reported to account for about 7% of female malignant tumours and 20% to 30% of female genital system malignant tumours. Accumulating evidence showed the expression of human trophoblast cell surface antigen 2 (Trop2) wa...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3450
更新日期:2020-03-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::The main purpose of the present investigation was to study the effect of cloturin on aerobic glycolysis, endogenous and exogenous respiration and the level of ATP in both Ehrlich ascites carcinoma (EAC) and P388 murine leukaemia cells incubated in vitro. Also its effect on the level of total (T-SH) and non-protein (NP...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100208
更新日期:1992-06-01 00:00:00
abstract::Organochalcogens are extensively produced and employed by industry and agriculture, and the risk of occupational and environmental toxicity to them has been poorly understood. Here, we investigated the acute effect of a new organochalcogen 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on biochemical and hematologic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2806
更新日期:2012-06-01 00:00:00
abstract::In response to concanavalin A, cytoplasmic calcium movement was observed in human platelets, both in the presence of 1 mM Ca2+ or 1 mM EGTA in the medium. Concanavalin A also caused the activation of inositide turnover and the production of inositol phosphates, suggesting that activation of phospholipase C occurs. The...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290100109
更新日期:1992-03-01 00:00:00
abstract::This study was designed to investigate the effect of L-carnitine in ischaemia and reperfusion of the rat kidney. Rats were randomly allocated into three groups. Group I (control group; n = 6) received no treatment. Group II (isotonic saline group; n = 6), received 2 ml of isotonic saline 15 min before the renal ischae...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1159
更新日期:2005-05-01 00:00:00
abstract::Rat intestinal epithelial cells were isolated by EDTA-chelation, combined with gentle shaking (modified Weiser procedure) or with strong longitudinal vibration (Harrison/Webster procedure). Both methods yield large numbers of viable cells and are relatively easy to use. Electronmicroscopical and biochemical data indic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020208
更新日期:1984-04-01 00:00:00
abstract::The flavanone hesperetin is known to decrease basal glucose uptake, although the inhibitory mechanism is largely unknown. Here, we used MDA-MB-231 breast cancer cells to investigate the molecular pathways affected by hesperetin. The results indicate that the suppression of glucose uptake is caused by the down-regulati...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2905
更新日期:2013-07-01 00:00:00
abstract::Ceramide is known to trigger apoptosis of nucleated cells and eryptosis of erythrocytes. Eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Besides ceramide, stimulators of eryptosis include increase of cytosolic Ca(2+) -activity ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3196
更新日期:2016-07-01 00:00:00
abstract::We examined the influence of chronic treatment with KN-93 (an inhibitor of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), 5 mg/kg given every other day for 4 weeks) on mean arterial pressure (MAP), urine protein and vascular reactivity in streptozotocin (STZ)-induced diabetes in Wistar Kyoto (WKY) and spontan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1446
更新日期:2008-03-01 00:00:00
abstract::Mesenchymal epithelial transition (C-MET) factor overexpression has been found in many types of cancer and has served as an important molecular target for therapeutic intervention. However, the role of C-MET in retinoblastoma remains largely unclear. The present study aimed to investigate the potential role and mechan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3464
更新日期:2020-03-01 00:00:00
abstract::Reduced and oxidized glutathione (GSH and GSSG), protein-bound glutathione, lipid peroxidation and antioxidant enzyme activities were determined in the erythrocyte lysates and membranes of type I and II alcoholics in order to clarify the effect of age-of-onset and the duration of the alcohol consumption on erythrocyte...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1125
更新日期:2005-01-01 00:00:00
abstract::Liver-directed gene therapy has become a promising treatment for many liver diseases. In this study, we constructed a multi-functional targeting molecule, which maintains targeting, endosome-escaping, and DNA-binding abilities for gene delivery. Two single oligonucleotide chains of Melittin (M) were synthesized. The f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2958
更新日期:2013-12-01 00:00:00
abstract::Rat liver nonparenchymal cells (NPC) were prepared by pronase digestion and purified on discontinuous gradients on Nycodenz. Morphological and biochemical characterization of cell suspensions showed that they were free of contamination by hepatocytes. We have confirmed the usefulness of pyruvate kinase activity in mon...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290070406
更新日期:1989-10-01 00:00:00
abstract::The levels of polyamines and their synthesizing enzymes in squamous cell carcinoma of prostate implanted in intact as well as castrated male rats were determined after certain hormonal manipulations. The tumour was found to grow with an identical rate in non-castrated and castrated rats. Polyamine content and activiti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030305
更新日期:1985-07-01 00:00:00