Abstract:
:Ceramide is known to trigger apoptosis of nucleated cells and eryptosis of erythrocytes. Eryptosis is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Besides ceramide, stimulators of eryptosis include increase of cytosolic Ca(2+) -activity ([Ca(2+) ]i ) and oxidative stress. Ceramide is degraded by acid ceramidase and inhibition of the enzyme similarly triggers apoptosis. The present study explored, whether ceramidase inhibitor Ceranib-2 induces eryptosis. Flow cytometry was employed to quantify phosphatidylserine-exposure at the cell surface from annexin-V-binding, cell volume from forward scatter, [Ca(2+) ]i from Fluo3-fluorescence, reactive oxygen species (ROS) from DCF dependent fluorescence, and ceramide abundance utilizing specific antibodies. Hemolysis was estimated from hemoglobin concentration in the supernatant. A 48 h exposure of human erythrocytes to Ceranib-2 significantly increased the percentage of annexin-V-binding cells (≥50 μM) and the percentage of hemolytic cells (≥10 μM) without significantly modifying forward scatter. Ceranib-2 significantly increased Fluo3-fluorescence, DCF fluorescence and ceramide abundance. The effect of Ceranib-2 on annexin-V-binding was not significantly blunted by removal of extracellular Ca(2+) . Ceranib-2 triggers phospholipid scrambling of the erythrocyte cell membrane, an effect at least in part due to increase of ceramide abundance and induction of oxidative stress, but not dependent on Ca(2+) entry. Copyright © 2016 John Wiley & Sons, Ltd.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Signoretto E,Zierle J,Bhuyan AA,Castagna M,Lang Fdoi
10.1002/cbf.3196subject
Has Abstractpub_date
2016-07-01 00:00:00pages
359-66issue
5eissn
0263-6484issn
1099-0844journal_volume
34pub_type
杂志文章abstract::Lipopolysaccharide (LPS)-induced inflammatory factors production by the cerebral cortical glial cells in two sides of the murine brain are different. To determine if microglial cells, a subset of glial cells, are involved in asymmetric production, interleukin-6 (IL-6), interleukin-1β (IL-1β) and nitric oxide (NO) resp...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1718
更新日期:2011-01-01 00:00:00
abstract::The dietary administration of selenium (sodium selenite; 4 p.p.m.) daily has been found to be highly effective in reducing the incidence of cancer induced by N-nitrosodiethylamine (DEN) in Wistar strain rats. Selenium treatment either before initiation, during initiation and selection/phenobarbital promotion phases of...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.979
更新日期:2002-12-01 00:00:00
abstract::Mitochondrial, endoplasmic reticular and plasma membrane fractions were isolated by a new method from control male Fischer 344 rats and rats given CCl4 by gavage. After 1 h of CCl4 treatment, rats were in glucose and pancreatic hormone balance but plasma levels of T3 and T4 were decreased 29 and 22%, respectively. Aft...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.934
更新日期:2002-03-01 00:00:00
abstract::The melastatin-related transient receptor potential channel TRPM2 is a plasma membrane Ca(2+)-permeable cation channel that is activated by hydrogen peroxide (H(2)O(2)) as a consequence of oxidative stress although the channel activation by H(2)O(2) appears to represent a cell-specific process in cells with endogenous...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1310
更新日期:2007-07-01 00:00:00
abstract::Glioblastoma is one of the most malignant tumors and causes the high mortality in cancer patients. Currently, there is no highly efficient therapy against glioblastoma. Therefore, searching for a new molecular target to anti-glioblastoma therapy is urgent and necessary. In this study, we elucidated the role of Signal ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3518
更新日期:2020-07-01 00:00:00
abstract::The study aims to evaluate the effect of saxagliptin, a specific inhibitor of dipeptidyl peptidase-4 enzymes, on body weight gain, lipid profiles, and cell death through apoptosis in rats with insulin resistance (IR). Male adult Sprague-Dawley rats (n = 32) were divided into 4 groups: control (Ctrl), IR, saxagliptin c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3333
更新日期:2018-06-01 00:00:00
abstract::Extracellular acylglycerols are hydrolysed by lipases active at the surface of intact fat cells isolated from rat or human adipose tissue. During short-term incubation, rat fat cells hydrolyse di-[3H]oleyl-[14C]glycerol at a rate of 70 +/- 7.7 mU/10(6) cells (mean +/- S.E.) versus 440 +/- 62 mU/10(6) cells for the hyd...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290050304
更新日期:1987-07-01 00:00:00
abstract::Gout is an inflammatory joint disorder characterized by hyperuricaemia and precipitation of monosodium urate crystals in the joints. In the present study, we aimed to investigate the anti-inflammatory effect of trikatu, a herbal compound in monosodium urate crystal-induced inflammation in rats, an experimental model f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2979
更新日期:2014-01-01 00:00:00
abstract::Human cytochrome P450 2J2 (CYP2J2) is abundant in cardiovascular tissue and active in the metabolism of arachidonic acid to eicosanoids that have potent vasodilatory properties. Variability of the CYP2J2 gene is highly constrained except for its proximal promoter: there is a relatively common and functionally relevant...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1512
更新日期:2008-10-01 00:00:00
abstract::The flavanone hesperetin is known to decrease basal glucose uptake, although the inhibitory mechanism is largely unknown. Here, we used MDA-MB-231 breast cancer cells to investigate the molecular pathways affected by hesperetin. The results indicate that the suppression of glucose uptake is caused by the down-regulati...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2905
更新日期:2013-07-01 00:00:00
abstract::Cardiovascular diseases are known to be the most fatal diseases worldwide. Ischaemia/reperfusion (I/R) injury is at the centre of the pathology of the most common cardiovascular diseases. According to the World Health Organization estimates, ischaemic heart disease is the leading global cause of death, causing more th...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3587
更新日期:2020-09-06 00:00:00
abstract::This study was designed to investigate the role of HO-1 induction in prevention of thioacetamide (TAA)-induced oxidative stress, inflammation and liver damage. The changes in hepatic dimethylarginine dimethylaminohydrolase (DDAH) activity as well as plasma arginine and asymmetric dimethylarginine (ADMA) levels were al...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2866
更新日期:2013-03-01 00:00:00
abstract::The influence of the 1,4-dihydropyridines (DHPs), water-soluble glutapyrone available as sodium, potassium and ammonium salts of 2-(2,6-dimethyl-3,5-diethoxycarbonyl-1,4-DHP-4-carboxamide)glutaric acid, from one side, and a lipophylic cerebrocrast, 2-propoxyethyl 2,6-dimethyl-4-(2-difluoromethoxyphenyl)-1,4-DHP-3,5-di...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199709)15:3<211::AID-CBF74
更新日期:1997-09-01 00:00:00
abstract::The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290130110
更新日期:1995-03-01 00:00:00
abstract::Although techniques for purifying epidermal stem cells (ESCs) have been established, enriching a pure population of viable ESCs is still a challenging task. One approach toward enhancing the purity and viability of ESCs involves cell markers. While evidence suggests that p63 plays a role in maintaining the population ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1612
更新日期:2009-12-01 00:00:00
abstract::The aim of this experimental study was to investigate the possible role of adenosine deaminase (AD) and xanthine oxidase (XO) in the pathogenesis of cisplatin-induced nephrotoxicity and the effect of erdosteine in decreasing the toxicity. The intraperitoneal injection of cisplatin (7 mg kg(-1) body weight) induced a s...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1069
更新日期:2004-05-01 00:00:00
abstract::Mesenchymal epithelial transition (C-MET) factor overexpression has been found in many types of cancer and has served as an important molecular target for therapeutic intervention. However, the role of C-MET in retinoblastoma remains largely unclear. The present study aimed to investigate the potential role and mechan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3464
更新日期:2020-03-01 00:00:00
abstract::After treatment of HD3 cells with erythroid-inducing agents (hemin and butyric acid) at 42 degrees C, the profile of phosphotyrosine-containing proteins was altered. Upon induction the overall level of phosphotyrosine-containing proteins increased. To examine the role of protein phosphorylation in HD3 cells differenti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(1998120)16:4<277::AID-CBF7
更新日期:1998-12-01 00:00:00
abstract::Sonic hedgehog (Shh) plays important roles in developmental of vertebrate animal central nervous system (CNS), and Gli is its downstream signal molecule. Shh signalling is essential for pattern formation, cell-fate specification, axon guidance, proliferation, survival and differentiation of neurons in CNS development....
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3582
更新日期:2020-08-25 00:00:00
abstract::Familial Mediterranean Fever (FMF) is an autosomal recessive hereditary disease leading mostly to renal failure and nephrotic syndrome. The ultrastructure of kidney has not been fully investigated in FMF associated renal disease. The aim of this study is to provide further evidence on the ultrastructure of kidney in p...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1286
更新日期:2007-07-01 00:00:00
abstract::The effects of 1,25-dihydroxyvitamin D3 are mediated by binding to a specific intracellular vitamin D receptor (VDR), which has been identified in a variety of tissues. Certain polymorphisms in the VDR gene have been associated with various neoplasms. For this purpose, we studied whether VDR TaqI or FokI genotype are ...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1386
更新日期:2007-11-01 00:00:00
abstract::Sexually-transmitted diseases (STD) can facilitate the progression of HIV-1 infection. Among them, as we have previously demonstrated, cervico-vaginal dysplasia-papillomavirus (HPV)-induced, together with HSV-2 co-infection, seems to be correlated with a more evident immunodepression in HIV-positive women, compared wi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.950
更新日期:2002-09-01 00:00:00
abstract::Systemic sclerosis (SSc) is an autoimmune disease characterized by inflammation and fibrosis of the skin and visceral organs. Fibrosis associated with SSc is characterized by an increased synthesis of a wide range of extracellular matrix (ECM). TGF-beta is a pluripotent cytokine in a wide range of cell types. In parti...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1649
更新日期:2010-06-01 00:00:00
abstract::The immunological induction of arthritis in the knee of the rabbit is well established as a model for human rheumatoid arthritis. It has the special advantage of allowing the development of the condition, and the effect of disease-modifying agents, to be followed. Attention has been focussed on the activity of glucose...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290080406
更新日期:1990-10-01 00:00:00
abstract::The vascular endothelium plays a key role in arterial wall homeostasis by preventing atherosclerotic plaque formation. A primary causal factor of endothelial dysfunction is the reactive oxygen species. Aerobic exercise is ascribed as an important adjuvant therapy in endothelium-dependent cardiovascular disease. Howeve...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1781
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to evaluate the role of mitochondria in the recovery of cardiac energetics induced by ischaemic preconditioning at reperfusion. Isolated rat hearts were aerobically perfused (control), subjected to global ischaemia and reperfusion (reperfusion), or subjected to 3 brief cycles of ischaemia/rep...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1294
更新日期:2006-11-01 00:00:00
abstract::Diabetes is associated with increased reactivity of the renal vascular bed to endothelin-1 (ET-1). It has been observed that diabetes is associated with over-expression of ET(A)- and ET(B)-receptors in the rat renal cortex. However it is not known if these receptors are over-expressed in the renal artery. The objectiv...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1253
更新日期:2006-09-01 00:00:00
abstract::Astroglial cells dispersed from newborn rat hemispheres were established in medium supplemented with 20 per cent fetal calf serum (FBS) and then grown to a confluent monolayer in the presence of 10 per cent FBS or charcoal-stripped FBS (CS). Type 1 astrocytes were subcultured and either maintained under the same condi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.645
更新日期:1996-03-01 00:00:00
abstract::The modification of myeloperoxidase and lactoperoxidase with 2-(O-methoxypolethylene glycol)-4, 6-dichloro-s-triazine, an activated polyethylene glycol (PEG1), was investigated. The modification caused a shift of the Soret band in the light absorption spectrum, from 430 nm to 418 nm in the case of myeloperoxidase (nat...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199806)16:2<123::AID-CBF77
更新日期:1998-06-01 00:00:00
abstract::We examined the influence of chronic treatment with KN-93 (an inhibitor of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), 5 mg/kg given every other day for 4 weeks) on mean arterial pressure (MAP), urine protein and vascular reactivity in streptozotocin (STZ)-induced diabetes in Wistar Kyoto (WKY) and spontan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1446
更新日期:2008-03-01 00:00:00