Abstract:
:Because of the demonstrated efficacy of Leu-M1 as a discriminant between malignant epithelioid mesothelioma (MEM) and adenocarcinomas involving the serosal surfaces (ACSs), the authors assessed the reactivities of related "myelomonocytic" antigens in this context. Paraffin sections from 41 MEMs and 43 ACSs (pulmonary, "serous surface papillary," and metastatic mammary adenocarcinomas) were evaluated for their expression of Leu-M1, LN1, LN2, and the Mac 387 antigen. Diagnoses were based in each case on the results of conventional histologic and electron microscopic examinations. Leu-M1 was detected only in minute foci of three peritoneal MEMs and was absent entirely in pleural mesotheliomas. Conversely, 38 of 43 ACSs expressed this marker. Three cases of peritoneal MEM and one pleural mesothelioma were multifocally LN2 positive, as were 39 of 43 ACSs. LN1 was the most frequently expressed antigen in MEM, being observed in 18 such tumors (10 pleural; 8 peritoneal); it was also detected in 37 of 43 ACSs. Mac 387 failed to label any of the neoplasms assessed in this series. These results demonstrate similar patterns of "myelomonocytic" antigen expression by diverse ACS and a general absence of Leu-M1 and LN2 in MEM. LN1 and the Mac 387 antigen appear to have no additional value when compared with Leu-M1 and LN2 in the immunohistochemical evaluation of serosal neoplasms.
journal_name
Am J Clin Patholjournal_title
American journal of clinical pathologyauthors
Wick MR,Mills SE,Swanson PEdoi
10.1093/ajcp/94.1.18subject
Has Abstractpub_date
1990-07-01 00:00:00pages
18-26issue
1eissn
0002-9173issn
1943-7722journal_volume
94pub_type
杂志文章abstract::This study was undertaken to evaluate the precision and sensitivity of three different commercial APTT reagents containing the activators kaolin, micronized silica, or ellagic acid. These reagents varied greatly in their ability to detect mild coagulopathies. The ellagic acid reagent was able to detect the mildest def...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/82.4.436
更新日期:1984-10-01 00:00:00
abstract::It has been shown that the monoclonal antibody Ki-67 reacts with a nuclear antigen that is expressed only by proliferating cells. The feasibility of using image analysis to quantitate Ki-67 staining (proliferation index [PI]) of epithelial ovarian cancers was investigated. The PI was determined in 50 advanced-stage pr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/99.6.736
更新日期:1993-06-01 00:00:00
abstract::High-performance liquid chromatography (HPLC) is replacing electrophoresis for identification of hemoglobin variants. Our objective was to identify unknown tall peaks with elution times and shapes of hemoglobin Barts found on hemoglobin chromatograms that could not be confirmed by alkaline and acid gel electrophoresis...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/73WJ-V2E0-RTVQ-H0BB
更新日期:2006-04-01 00:00:00
abstract::Human serum contains 2 isoforms of type-5 tartrate-resistant acid phosphatase (TRACP): 5a and 5b. TRACP-5b is osteoclastic. Our goal was to determine if serum TRACP-5a could originate from inflammatory macrophages (MPhi). We stained 246 paraffin-embedded tissue samples for TRACP using monoclonal antibody 9C5 (mab9C5) ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/DGEA9BE2VE5VCFYH
更新日期:2007-04-01 00:00:00
abstract::The development of sensitive immunofluorescent technics for the detection of platelet antibodies has stimulated renewed interest in the field of platelet immunology. However, the analysis of multispecific sera containing both HLA and platelet-specific antibodies requires cumbersome absorption technics and relatively l...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/80.6.839
更新日期:1983-12-01 00:00:00
abstract::The effects of physiologic temperature variations on antibiotic-induced killing of Escherichia coli, Pseudomonas aeruginosa, Streptococcus faecalis, and Streptococcus pneumoniae were studied. At concentrations less than or equal to four times the minimal bactericidal concentration, the activity of each of the antibiot...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/76.1.57
更新日期:1981-07-01 00:00:00
abstract:OBJECTIVES:Acute myeloid leukemia with myelodysplasia-related changes (AML-MRC) is a heterogeneous disorder defined by morphologic, genetic, or clinical features. Genetic abnormalities associated with AML-MRC are often associated with adverse prognostic features, and many cases are preceded by a myelodysplastic syndrom...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1309/AJCP58RSMFRHLHHH
更新日期:2015-07-01 00:00:00
abstract::An evaluation of the performance of duplicate prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays was undertaken to develop analytical duplicate performance criteria in order to quantitate the risks associated with singlet versus duplicate procedures. Data were retrospectively collected from ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/85.4.456
更新日期:1986-04-01 00:00:00
abstract::We performed a retrospective clinicopathologic study on sequential biopsy specimens from 90 patients with Philadelphia chromosome-positive chronic myelogenous leukemia to study therapy-specific effects of busulfan (28 patients), hydroxyurea (32 patients), and interferon-alfa (IFN-alfa; 30 patients). Bone marrow specim...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/XMGX-7HQ8-7PLU-LQ9M
更新日期:2000-07-01 00:00:00
abstract::The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPAH5WR2LFTUSI
更新日期:2011-12-01 00:00:00
abstract::Three paired samples were distributed at three--six-month intervals in the Basic Ligand Survey during 1976. The stabilities of mean values obtained by widely used commercial kit procedures and by all methods for eight analytes are reported. Significant improvements in accuracy and long-term precision were identified i...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::We report 8 cases of hepatosplenic T-cell lymphoma (HSTCL) involving bone marrow and correlate histologic findings with disease progression. Immunophenotypic analysis demonstrated mature, aberrant gamma/delta T-cell immunophenotypes. Isochromosome 7q was identified in 4 cases; 1 case showed the t(7;14)(q34;q13). Seven...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/BM40-YM6J-9T3X-MH8H
更新日期:2001-09-01 00:00:00
abstract:Objectives:To determine non-American Society for Clinical Pathology pathology- and laboratory-related Choosing Wisely recommendations that drive effective test utilization in the laboratory. Methods:Data were collected via a two-part web-based survey distributed to a broad sample of pathologists and laboratory profess...
journal_title:American journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1093/ajcp/aqx159
更新日期:2018-02-17 00:00:00
abstract:Objectives:To determine whether V H family usage in B-cell lymphoproliferative disorders can be deduced from polymerase chain reaction (PCR) product-length information obtained through the BIOMED-2 (Invivoscribe, San Diego, CA) clonality assay. Methods:We develop an algorithm that uses the sizing information of the BI...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqx007
更新日期:2017-06-01 00:00:00
abstract::Epithelial hyperplasia, a component of proliferative breast disease (PBD), is a known risk factor for the development of breast carcinoma. To determine if criteria established for tissue biopsy could be used to more precisely define cytologic criteria for diagnosis of PBD, 37 breast fine-needle aspirates with biopsy d...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/103.4.438
更新日期:1995-04-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH), using repetitive alpha satellite probes to chromosomes 9 and 17, was performed on 27 paraffinembedded bladder specimens from 18 Egyptian patients affected with bilharzial carcinoma. Fluorescence in situ hybridization results in the carcinomas and benign mucosa of patients wit...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.2.234
更新日期:1996-08-01 00:00:00
abstract::Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic in New York City hospitals and have been associated with serious infections globally. A real-time polymerase chain reaction (RT-PCR) assay was developed to detect carbapenem resistance attributable to KPC from blood culture bottles posi...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCP9SNHJG2QGLWU
更新日期:2012-04-01 00:00:00
abstract::Encephalitozoon (Septata) intestinalis affects AIDS patients with CD4 counts <100/microL, causing intestinal and disseminated disease. It must be distinguished from the more common intestinal microsporidian, Enterocytozoon bieneusi, and from other microsporidia of extraintestinal tissues, such as Encephalitozoon helle...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/106.5.606
更新日期:1996-11-01 00:00:00
abstract::The hematologic status of 265 patients with rheumatoid arthritis was assessed. In the group as a whole, a mild depression in the hemoglobin concentration and mean cell volume (MCV) was associated with an increase in the red blood cell distribution width (RDW), erythrocyte sedimentation rate (ESR), and platelet count. ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/87.2.196
更新日期:1987-02-01 00:00:00
abstract::This study investigates the dispensing and inoculating characteristics of the MIC-2000 micro-broth dilution system and compares its ability to determine minimum inhibitory concentrations with that of the reference macro-broth dilution method. Micro-well filling showed a coefficient of variation of 6.8%, and micro-well...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/73.3.374
更新日期:1980-03-01 00:00:00
abstract::Four commercially available herpes simplex virus 1 (HSV-1) indirect fluorescent antibody (IFA) kits were compared with the use of sera selected because they were negative for HSV antibody by complement fixation (CF less than 1:8) and by ELISA (less than 1:100). However, 14 of 24 (58.3%) of these HSV-1 antibody-negativ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/86.6.765
更新日期:1986-12-01 00:00:00
abstract::The authors report the clinicopathologic findings in four cases of adult women with rhabdomyosarcomas that originated in the endometrium or cervix, or both. All tumors were pure sarcomas and not components of carcinosarcomas or malignant mixed mesodermal tumors. In two patients, the tumors were typical botryoid embryo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/70.2.217
更新日期:1978-08-01 00:00:00
abstract::In order to further clarify the histologic criteria and prognostic measurement of appendiceal adenoma and well differentiated adenocarcinoma, lectin binding activity in 31 cases of appendiceal mucocele (6 hyperplasias, 16 adenomas and 9 adenocarcinomas) was studied using an immunoperoxidase staining method. An additio...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/89.4.481
更新日期:1988-04-01 00:00:00
abstract::The 1973 Comprehensive Blood Bank Survey Program of the College of American Pathologists was administered to 2,200 laboratories and blood banks throughout the United States. The results showed a considerable increase in the accuracy of the testing and in the performance of reagents. Accuracy of ABO testing was 99.3%; ...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
abstract::We studied methylation of 2 tumor suppressor genes (p14, p16) and 4 MINT (methylated in tumor) clones (MINT1, MINT2, MINT25, MINT31) among 51 fundic gland polyps (FGPs) and 27 normal gastric body biopsy samples using bisulfite treatment of genomic DNA followed by methylation-specific polymerase chain reaction. Thirty-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/4QUN-J4F2-7QK7-RR0G
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVES:Solid tumor metastases to the pancreas are rare, and only limited literature on the topic is available. In this retrospective study, we reviewed 107 cases of solid tumor metastases to the pancreas diagnosed by fine-needle aspiration (FNA) from 2005 to 2019. METHODS:Information including the patients' clinic...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqaa090
更新日期:2020-10-13 00:00:00
abstract::Recent reports show that the sensitivity of endometrial carcinoma detection on liquid-based Papanicolaou (Pap) tests (88%) is considerably higher than that reported on conventional Pap smears (20%-30%), although few laboratories have corroborated these results. We performed a 5-year retrospective review of all liquid-...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1309/AJCPLFBK1A2XJDQI
更新日期:2012-02-01 00:00:00
abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/94.6.687
更新日期:1990-12-01 00:00:00
abstract::Several technics are currently being used to detect hepatitis type A antigen or its antibody. These include immunoelectronmicroscopy, immune adherence, and complement fixation. This paper describes another promising technic, a microtiter solid-phase immunoradiometric assay, in which hepatitis A antigen and antibody ar...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:
更新日期:1976-05-01 00:00:00
abstract:Objectives:According to current guidelines, either immunohistochemistry (IHC) or in situ hybridization (ISH) can be used to determine human epidermal growth factor receptor 2 (Her2/neu) status in breast carcinoma. While the guidelines explicitly delineate result interpretation, there is no consensus on the most appropr...
journal_title:American journal of clinical pathology
pub_type: 杂志文章
doi:10.1093/ajcp/aqw224
更新日期:2017-04-01 00:00:00