Tartrate-resistant acid phosphatase as an immunohistochemical marker for inflammatory macrophages.

Abstract:

:Human serum contains 2 isoforms of type-5 tartrate-resistant acid phosphatase (TRACP): 5a and 5b. TRACP-5b is osteoclastic. Our goal was to determine if serum TRACP-5a could originate from inflammatory macrophages (MPhi). We stained 246 paraffin-embedded tissue samples for TRACP using monoclonal antibody 9C5 (mab9C5) to isoforms 5a and 5b and a novel mab220 specific to isoform 5a. CD68 and lysozyme were also stained. MPhi of chronic and granulomatous inflammation and in tissues that undergo strong antigenic stimulation were strongly positive for TRACP, more so with mab220 than with mab9C5. Noninflammatory MPhi in lymph node sinuses or germinal centers and red pulp MPhi of spleen were weak or negative for TRACP. Marginal zone lymphocytes and sebaceous glands of skin were weakly positive for TRACP. Tissue mast cells displayed strong TRACP staining. Neuroendocrine cells of gastrointestinal tissues were strongly immunoreactive with mab9C5 but negative with mab220. Restricted expression of TRACP primarily in inflammatory MPhi supports our hypothesis that circulating TRACP-5a could be a biomarker of chronic inflammatory disease activity.

journal_name

Am J Clin Pathol

authors

Janckila AJ,Slone SP,Lear SC,Martin A,Yam LT

doi

10.1309/DGEA9BE2VE5VCFYH

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

556-66

issue

4

eissn

0002-9173

issn

1943-7722

pii

248250116635L763

journal_volume

127

pub_type

杂志文章
  • Postmortem bacteriology and pneumonia in a mentally retarded population.

    abstract::Postmortem pathologic and bacteriologic reports were analyzed for 237 deaths occurring at a large institution for the mentally retarded from 1958 to 1973. Bronchopneumonia, aspiration pneumonia, and lipid pneumonia were frequently reported at autopsy, and in a total of 146 cases (61.6%) at least one type of pneumonia ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/67.2.190

    authors: Polednak AP

    更新日期:1977-02-01 00:00:00

  • Infections caused by Actinomyces viscosus.

    abstract::Two cases of Actinomyces viscosus infection of the lungs were seen in nonimmunosuppressed patients. One patient had a peripheral actinomycotic lung mass resembling a tumor. Both patients responded to a long course of penicillin therapy. Reports of A. viscosus infections are rare, although the organism colonizes the mo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.1.113

    authors: Eng RH,Corrado ML,Cleri D,Cherubin C,Goldstein EJ

    更新日期:1981-01-01 00:00:00

  • Relationship of pathologic factors to efficacy of sorafenib treatment in patients with metastatic clear cell renal cell carcinoma.

    abstract:OBJECTIVES:To evaluate the predictive value of growth patterns in patients undergoing sorafenib treatment for metastatic clear cell renal cell carcinomas (CCRCCs). METHODS:Forty-eight patients were analyzed, each of whom underwent nephrectomy and received sorafenib treatment for metastatic CCRCC. Progression-free surv...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPM8RJ2ECAWOGQ

    authors: Araki H,Tsuzuki T,Kimura T,Tanaka K,Yamada S,Sassa N,Yoshino Y,Hattori R,Gotoh M

    更新日期:2015-04-01 00:00:00

  • Breast carcinoma simulating fibroadenoma or fibrocystic change by fine-needle aspiration. A study of 16 cases.

    abstract::The cytologic features of fine-needle aspiration specimens from 16 breast carcinomas that closely simulated benign lesions were analyzed and compared to smears from fibroadenomas and fibrocystic change. No combination of features was found that accurately separated all benign and malignant cases. Many nuclei with disc...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/98.2.155

    authors: Rogers LA,Lee KR

    更新日期:1992-08-01 00:00:00

  • The Importance of Genetics Experts in Optimizing Genetic Test Orders Through Prospective and Retrospective Reviews.

    abstract:OBJECTIVES:To demonstrate the impact of genetics specialists on identifying test order errors and improving reimbursement for genetic testing. METHODS:Forty-four cases in which whole exome sequencing (WES) was performed but not reimbursed were reviewed by a genetic counselor through simulated prospective and retrospec...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqz188

    authors: Conway ME,Kalejta CD,Sternen DL,Singh IR

    更新日期:2020-03-09 00:00:00

  • Diversity of organ site involvement among malignant lymphomas of mucosa-associated tissues.

    abstract::Fifteen cases of low-grade B-cell lymphoma involving unusual extranodal sites have been studied in comparison to cases reported or observed arising in typical mucosa-associated lymphoid tissues. In every case, histopathologic features conformed to those characteristic for lymphomas of mucosa-associated lymphoid tissue...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.6.738

    authors: Pelstring RJ,Essell JH,Kurtin PJ,Cohen AR,Banks PM

    更新日期:1991-12-01 00:00:00

  • Utility of acid-fast staining for detection of mycobacteria in cutaneous granulomatous tissue reactions.

    abstract:OBJECTIVES:Ancillary molecular testing on tissue is available for mycobacterial disease; however, judicious use of highly sensitive tests, such as polymerase chain reaction (PCR) and sequencing, should be guided by histologic parameters. We sought to investigate the utility of performing acid-fast stains (AFS) on skin ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPNM3J9TOIBRSK

    authors: Laga AC,Milner DA Jr,Granter SR

    更新日期:2014-04-01 00:00:00

  • Ultrastructural morphometry of thyroid neoplasms.

    abstract::This paper presents the first comprehensive morphometry analysis of normal thyroid, adenomas, and follicular and papillary carcinomas. The mean nuclear volume and the mean nuclear surface increased, while the volume densities of rough endoplasmic reticulum and dense bodies decreased from normal thyroid through adenoma...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.2.166

    authors: Johannessen JV,Sobrinho-Simóes M,Finseth I,Pilström L

    更新日期:1983-02-01 00:00:00

  • Effects of chemotherapy (busulfan-hydroxyurea) and interferon-alfa on bone marrow morphologic features in chronic myelogenous leukemia. Histochemical and morphometric study on sequential trephine biopsy specimens with special emphasis on dynamic features.

    abstract::We performed a retrospective clinicopathologic study on sequential biopsy specimens from 90 patients with Philadelphia chromosome-positive chronic myelogenous leukemia to study therapy-specific effects of busulfan (28 patients), hydroxyurea (32 patients), and interferon-alfa (IFN-alfa; 30 patients). Bone marrow specim...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/XMGX-7HQ8-7PLU-LQ9M

    authors: Thiele J,Kvasnicka HM,Schmitt-Graeff A,Bundschuh S,Biermann T,Roessler G,Wasmus M,Diehl V,Zankovich R,Schaefer HE

    更新日期:2000-07-01 00:00:00

  • Use of commercially prepared control sera as quality control materials for spectrophotometric bilirubin determinations in amniotic fluid.

    abstract::The visible absorption spectra of dilutions of commercially prepared chemistry control sera closely mimic those of jaundiced amniotic fluids in cases of fetal rhesus isoimmunization. The authors have assessed the use of these materials for the quality control of net absorbance measurements at 450 nm on amniotic fluids...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.4.513

    authors: McDonald OL,Watts MT

    更新日期:1985-10-01 00:00:00

  • Evaluation of two commercial tests for human immunodeficiency virus antigens in culture supernatant fluid.

    abstract::Two commercial enzyme-linked immunosorbent assays (EIAs) for human immunodeficiency virus (HIV) antigens were evaluated for sensitivity and reproducibility by use of supernatant fluid from cell cultures of peripheral mononuclear cells of 18 patients with acquired immunodeficiency syndrome (AIDS) and 12 asymptomatic HI...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/89.6.788

    authors: Jackson JB,Sannerud K,Rhame FS,Balfour HH Jr

    更新日期:1988-06-01 00:00:00

  • Sarcomatoid carcinoma (so-called pseudosarcoma) of the larynx simulating malignant giant cell tumor of soft parts. A case report.

    abstract::A tumor of the larynx composed of a small infiltrating and well-differentiated squamous cell carcinoma and a large polypoid sarcomatous mass showing the histologic features of the malignant giant cell tumor of soft parts (MGCT) is reported. Other types of sarcomatous components have been described with squamous cell c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/82.3.340

    authors: Alguacil-Garcia A,Alonso A,Pettigrew NM

    更新日期:1984-09-01 00:00:00

  • Hepatosplenic gamma/delta T-cell lymphoma in bone marrow. A sinusoidal neoplasm with blastic cytologic features.

    abstract::We report 8 cases of hepatosplenic T-cell lymphoma (HSTCL) involving bone marrow and correlate histologic findings with disease progression. Immunophenotypic analysis demonstrated mature, aberrant gamma/delta T-cell immunophenotypes. Isochromosome 7q was identified in 4 cases; 1 case showed the t(7;14)(q34;q13). Seven...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/BM40-YM6J-9T3X-MH8H

    authors: Vega F,Medeiros LJ,Bueso-Ramos C,Jones D,Lai R,Luthra R,Abruzzo LV

    更新日期:2001-09-01 00:00:00

  • Direct comparison of two mechanized systems for identification of gram-negative bacilli. Autobac ID system versus the auto microbic system (with EBC plus).

    abstract::The Auto Microbic System (AMS) is an almost completely automated system, capable of identifying Enterobacteriaceae after 8 hours, and some glucose nonfermenters after 13 hours of incubation. The Autobac ID system is a mechanized, computer-assisted system capable of identifying Enterobacteriaceae and many nonfermentati...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/78.4.462

    authors: Barry AL,Gavan TL,Badal RE,Telenson MJ

    更新日期:1982-10-01 00:00:00

  • Analysis of human T-cell lymphotropic virus in CD25+ anaplastic large cell lymphoma in children.

    abstract::Anaplastic large cell lymphoma (ALCL) is recognized as 2 distinct diseases: anaplastic lymphoma kinase (ALK)+ ALCL and ALK- ALCL. ALK+ ALCL occurs in younger patients and has a better prognosis. Human T-cell lymphotropic virus (HTLV-1) is linked to the development of adult T-cell leukemia/lymphoma (ATLL), which freque...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP6Q7QMUVGMVMF

    authors: Gualco G,Chioato L,Weiss LM,Harrington WJ Jr,Bacchi CE

    更新日期:2009-07-01 00:00:00

  • Susceptibilities of Pseudomonas species to tetracycline, minocycline, gentamicin, and tobramycin.

    abstract::Members of the genus Pseudomonas are widely implicated in human disease. Although most isolates are P. aeruginosa, there have been serious outbreaks of infection with other members of the genus. The susceptibility patterns of 5) Pseudomonas isolates are reported. It was notable that of the four antibiotics tested, uni...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/69.4.410

    authors: Tilton RC,Steingrimsson O,Ryan RW

    更新日期:1978-04-01 00:00:00

  • The utility of BRAF V600E mutation-specific antibody VE1 for the diagnosis of hairy cell leukemia.

    abstract:OBJECTIVES:BRAF V600E mutation is characteristic of hairy cell leukemia (HCL). A V600E mutation-specific antibody, VE1, has been recently developed. We studied the diagnostic utility of this antibody in HCL and compared it with other B-cell neoplasms. METHODS:VE1 activity was assessed using immunohistochemistry in 90 ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPQLQ89VXTVWKN

    authors: Uppal G,Ly V,Wang ZX,Bajaj R,Solomides CC,Banks PM,Medeiros LJ,Peiper SC,Gong JZ

    更新日期:2015-01-01 00:00:00

  • Phenotypic and genotypic characterization of carcinomas of the papilla of Vater has prognostic and putative therapeutic implications.

    abstract::We further characterize the heterogeneous carcinomas of the papilla of Vater (CPVs) in relation to various clinicopathologic patient characteristics and patient survival. Of the 71 reevaluated CPVs, 32 were intestinal, 26 were pancreatobiliary, 6 were mixed, 4 were mucinous, and 3 were poorly differentiated carcinomas...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPCTCUQSYI89YT

    authors: Kohler I,Jacob D,Budzies J,Lehmann A,Weichert W,Schulz S,Neuhaus P,Röcken C

    更新日期:2011-02-01 00:00:00

  • Cytologic features of poorly differentiated 'insular' carcinoma of the thyroid, as revealed by fine-needle aspiration biopsy.

    abstract::The authors report on the cytologic features of six cases of poorly differentiated "insular" carcinoma (IC) of the thyroid, a recently described variety of thyroid tumor intermediate between well-differentiated and anaplastic neoplasms. It is characterized by trabecular and/or alveolar growth patterns, merging with fo...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/94.6.687

    authors: Pietribiasi F,Sapino A,Papotti M,Bussolati G

    更新日期:1990-12-01 00:00:00

  • Secondary CD5+ diffuse large B-cell lymphoma not associated with transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma (Richter syndrome).

    abstract::Few cases of secondary CD5+ diffuse large B-cell lymphoma (DLBCL) that are not Richter syndrome have been reported previously. We report 9 cases of non-Richter syndrome secondary CD5+ DLBCL. Among 529 cases of DLBCL, 38 (7.2%) were CD5+ DLBCL, including 9 of secondary CD5+ DLBCL. Five cases gained CD5 expression durin...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP58FETFGLCKKW

    authors: Maeshima AM,Taniguchi H,Nomoto J,Maruyama D,Kim SW,Watanabe T,Kobayashi Y,Tobinai K,Matsuno Y

    更新日期:2009-03-01 00:00:00

  • Modified Plasma-Thrombin Method of Cell Block Preparation for Fine-Needle Aspiration Biopsies in Resource-Limited Settings.

    abstract:Objectives:The plasma-thrombin method is commonly used to make cell blocks from fine-needle aspiration (FNA) samples but requires centrifugation. We describe a modification to this method that does not require centrifugation for use in resource-limited settings. Methods:Pooled fresh plasma is aliquoted into 2-mL Eppen...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqy031

    authors: Aisagbonhi O,Birungi A,Atwine R,Behayo P,Ayebaziwe B,Roberts D,Tambouret R

    更新日期:2018-07-03 00:00:00

  • Prevalence of RhD variants, confirmed by molecular genotyping, in a multiethnic prenatal population.

    abstract::RhD determination in pregnant women is critical to facilitate Rh immune globulin prophylaxis for RhD-negative women. A single amino acid change in the RhD antigen can cause epitope loss, giving rise to "partial D" variants. Women with some partial D variants may develop anti-D against the missing epitope after pregnan...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPSXN9HQ4DELJE

    authors: Wang D,Lane C,Quillen K

    更新日期:2010-09-01 00:00:00

  • Susceptibility testing with anaerobic blood culture isolates. Comparison of a rapid, direct methods with standardized method.

    abstract::The results of a direct anaerobic susceptibility testing method were compared with those of a standardized method. Seventy-three blood culture isolates and eight antimicrobial agents were used. The results of the direct and standardized testing methods were within one two-fold dilution for 95.2% of the 584 anaerobe-an...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/73.4.558

    authors: Murray PR,Christman JL

    更新日期:1980-04-01 00:00:00

  • Automation in clinical microbiology.

    abstract::Automation was introduced into the clinical microbiology laboratory in the 1960s but initially met with limited success. Today, instruments are an integral part of many clinical laboratories and are used for microbial detection, identification, and susceptibility testing; detection of positive blood cultures; screenin...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:

    authors: Woods GL

    更新日期:1992-10-01 00:00:00

  • Spurious hemoglobin Barts caused by bilirubin: a common interference mimicking an uncommon hemoglobinopathy.

    abstract::High-performance liquid chromatography (HPLC) is replacing electrophoresis for identification of hemoglobin variants. Our objective was to identify unknown tall peaks with elution times and shapes of hemoglobin Barts found on hemoglobin chromatograms that could not be confirmed by alkaline and acid gel electrophoresis...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/73WJ-V2E0-RTVQ-H0BB

    authors: Howanitz PJ,Kozarski TB,Howanitz JH,Chauhan YS

    更新日期:2006-04-01 00:00:00

  • Detection of enteroviral infection in paraffin-embedded tissue by the RNA polymerase chain reaction technique.

    abstract::A method using the RNA polymerase chain reaction technique to diagnose infection retrospectively using single-stranded RNA enteroviruses in paraffin-embedded tissue blocks is reported. This method takes advantage of extreme sequence conservation in the 5' untranslated region of the enteroviral genome and uses two roun...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/96.5.568

    authors: Redline RW,Genest DR,Tycko B

    更新日期:1991-11-01 00:00:00

  • Transfusion-related acute lung injury: a case-control pilot study of risk factors.

    abstract::Transfusion-related acute lung injury (TRALI) is the leading cause of mortality from transfusion therapy. Recipient, donor, and blood product risk factors may have important roles in the occurrence of TRALI. A case-control pilot study of 6 TRALI cases in which HLA-antibody concordance was found and 20 control subjects...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/HC4PVY24NJXMQ884

    authors: Sanchez R,Bacchetti P,Toy P

    更新日期:2007-07-01 00:00:00

  • Squamous cell carcinoma variants of the upper aerodigestive tract.

    abstract::Squamous cell carcinoma (SCC) is by far the most common malignancy of the upper aerodigestive tract. Most conventional-type SCCs do not present the surgical pathologist with diagnostic difficulty. Certain variants, however, can histologically mimic benign and other malignant diseases. In such cases, correct diagnosis ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/CR5JXUY3J2YGTC1D

    authors: Stelow EB,Mills SE

    更新日期:2005-12-01 00:00:00

  • Percutaneous ultrasound-guided needle biopsy of hepatic mass lesions using a cytohistologic approach. Comparison of two needle types.

    abstract::This study compared the ability to diagnose and accurately classify hepatic mass lesions by ultrasound-guided biopsy using two different needle types: a 20 gauge needle that yielded primarily cytologic material and an 18 gauge automated core biopsy needle. Slides prepared from each of the needle types were separately ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/104.5.583

    authors: Dusenbery D,Ferris JV,Thaete FL,Carr BI

    更新日期:1995-11-01 00:00:00

  • Expression of the serpin centerin defines a germinal center phenotype in B-cell lymphomas.

    abstract::Centerin (SERPINA9/GCET1) is a protease inhibitor with expression restricted to germinal center B cells and lymphoid malignancies with germinal center B-cell maturation. Expression of the centerin gene transcript, along with bcl-6 and GCET2/HGAL, constitutes a molecular signature associated with a good prognosis in di...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/9QKE68QU7B825A3U

    authors: Paterson MA,Hosking PS,Coughlin PB

    更新日期:2008-07-01 00:00:00