Utility of acid-fast staining for detection of mycobacteria in cutaneous granulomatous tissue reactions.

Abstract:

OBJECTIVES:Ancillary molecular testing on tissue is available for mycobacterial disease; however, judicious use of highly sensitive tests, such as polymerase chain reaction (PCR) and sequencing, should be guided by histologic parameters. We sought to investigate the utility of performing acid-fast stains (AFS) on skin biopsy specimens with granulomatous inflammation without an otherwise obvious histologic or clinical explanation. METHODS:Our retrospective review identified 31 patients with biopsy specimens showing granulomatous inflammation that had simultaneous AFS and mycobacterial culture or PCR performed. RESULTS:Biopsy specimens from eight (25.8%) patients had AFS interpreted as positive or suspicious for acid-fast bacilli. Eight had positive cultures and one had positive PCR. One biopsy specimen with AFS that showed occasional acid-fast structures that were interpreted as "suspicious" for mycobacteria was associated with a negative culture and negative PCR. Three (9.7%) biopsy specimens with negative AFS had positive cultures, and 19 (61.3%) biopsy specimens with negative AFS also had negative culture results. In our biopsy specimens, sensitivity of AFS was 72.7% and specificity was 95.0%. Positive predictive value of AFS was 88.9%, and negative predictive value was 86.4%. CONCLUSIONS:AFS has good sensitivity and excellent specificity and should be performed on all unexplained granulomatous tissue reactions of skin in conjunction with mycobacterial culture.

journal_name

Am J Clin Pathol

authors

Laga AC,Milner DA Jr,Granter SR

doi

10.1309/AJCPNM3J9TOIBRSK

subject

Has Abstract

pub_date

2014-04-01 00:00:00

pages

584-6

issue

4

eissn

0002-9173

issn

1943-7722

pii

141/4/584

journal_volume

141

pub_type

杂志文章
  • Detection of bacteremia with the BACTEC 16B resin blood culture medium.

    abstract::A new blood culture medium (16B) containing adsorbent and cationic exchange resins has become available for use with the BACTEC instrument (Johnston Laboratories, Towson, MD). Its purpose is to enhance the detection of bacteremia through binding of antimicrobials. The performance of the BACTEC 16B resin medium was com...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.5.643

    authors: DeGirolami PC,Eichelberger K,Siegel J

    更新日期:1984-05-01 00:00:00

  • Atypical glandular cells of undetermined significance. Diagnostic accuracy and interobserver variability using select cytologic criteria.

    abstract::Histologic follow-up of patients with the Bethesda system cervical-vaginal diagnosis of atypical glandular cells of undetermined significance (AGUS), "favor endocervical origin", or "not otherwise specified" (NOS) shows a high percentage of clinically significant (neoplastic or preneoplastic) lesions. Using the criter...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/107.3.299

    authors: Raab SS,Snider TE,Potts SA,McDaniel HL,Robinson RA,Nelson DL,Sigman JD,Thomas PA

    更新日期:1997-03-01 00:00:00

  • A rapid paper-disc test for penicillinase.

    abstract::A practical acidimetric assay for penicillinase production using penicillin and phenol red impregnated in paper discs was developed. Blank discs were first impregnated with buffered penicillin G and, after drying, with 1% phenol red. For use, a disc was added to a bacterial suspension made in saline solution. A yellow...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/75.4.557

    authors: Tu KK,Jorgensen JH,Stratton CW

    更新日期:1981-04-01 00:00:00

  • Estimation of PR and ER by immunocytochemistry in breast cancer. Comparison with radioligand binding methods.

    abstract::Immunocytochemical assays for progesterone receptor (PR) using monoclonal antirabbit PR antibodies (PR-ICA) and for estrogen receptor (ER) (ER-ICA) were compared with radioligand binding (dextran-coated charcoal [DCC]) methods for receptor determination in patients with breast cancer. Immunocytochemical staining for P...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:

    authors: Seymour L,Meyer K,Esser J,MacPhail AP,Behr A,Bezwoda WR

    更新日期:1990-10-01 00:00:00

  • A study of results generated using the Abbott LCx-GC assay fails to reveal a performance-based rationale for the 2002 level 1 recall.

    abstract::To establish the effect of a quality control failure on the performance of the LCx-GC nucleic-acid amplification assay for Neisseria gonorrhoeae (Abbott Laboratories, Abbott Park, IL) in the field, we conducted a retrospective analysis comparing the clinical and analytic performance of the recalled lots with those not...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/1LD8-LU7Y-FFW7-5WQA

    authors: Skeate RC,Wahi MM,Hanson KL,Cartwright CP

    更新日期:2005-06-01 00:00:00

  • Evaluation of the Abbott CELL-DYN 4000 hematology analyzer.

    abstract::A new generation hematology analyzer, Abbott CELL-DYN 4000 (CD 4000), capable of providing 26 parameters, including fully automated reticulocyte, nucleated RBC, blast, band, and immature granulocyte, and variant lymphocyte counts, was evaluated by using the National Committee for Clinical Laboratory Standards H20-A pr...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/L7LC-6KKJ-12G2-196W

    authors: Grimaldi E,Scopacasa F

    更新日期:2000-04-01 00:00:00

  • Prediction of carrier status in Duchenne dystrophy by creatine kinase measurement.

    abstract::Serum creatine kinase (CK) was measured in 515 healthy white women and 28 obligate carriers for Duchenne muscular dystrophy. There was substantial overlap between the control and carrier populations. To analyze the impact of the degree of overlap, the predictive value of a CK result was determined by (1) using sensiti...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/84.5.655

    authors: Gruemer HD,Miller WG,Chinchilli VM,Leshner RT,Blasco PA,Hassler CR,Nance WE

    更新日期:1985-11-01 00:00:00

  • Recombinant factor VIIa in the treatment of bleeding.

    abstract::Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. It has become apparent that rFVIIa is useful in controlling bleeding in a variety of clinical situations. This review attempts to collate and summarize the nonhemophilia...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/D0G0-C96V-05CJ-5B4J

    authors: Midathada MV,Mehta P,Waner M,Fink LM

    更新日期:2004-01-01 00:00:00

  • To step or not to step: an approach to clinically diagnosed polyps with no initial pathologic finding.

    abstract::We determined whether there were additional diagnostic findings in additional level sections performed on polyps with no pathologic diagnosis (NPD) or those in which only lymphoid aggregates (LAs) were seen initially and determined the level at which findings were identified. All colorectal biopsy specimens submitted ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/27NQ-A1R2-DQ41-20Y7

    authors: Nash JW,Niemann T,Marsh WL,Frankel WL

    更新日期:2002-03-01 00:00:00

  • Development of North American consensus guidelines for medical laboratories that perform and interpret platelet function testing using light transmission aggregometry.

    abstract::Platelet function testing is important for the diagnostic evaluation of common and rare bleeding disorders. Our study goals were to promote best practices and reduce unnecessary testing variances by developing North American guidelines on platelet function testing. Guidelines were developed by consensus for expert rec...

    journal_title:American journal of clinical pathology

    pub_type: 共识发展会议,杂志文章,实务指引

    doi:10.1309/AJCP9V3RRVNZMKDS

    authors: Hayward CP,Moffat KA,Raby A,Israels S,Plumhoff E,Flynn G,Zehnder JL

    更新日期:2010-12-01 00:00:00

  • The effect of fibrin polymerization inhibitors on quantitative measurements of plasma fibrinogen.

    abstract::A number of reports have highlighted discrepancies between the DuPont aca and fibrometer-based methods for quantitating plasma fibrinogen levels. Although many authors have suggested that the presence of fibrin(ogen) degradation products are in some way responsible for the discrepancies, no direct test of this hypothe...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/88.4.490

    authors: Hoffman M,Greenberg CS

    更新日期:1987-10-01 00:00:00

  • Large colorectal adenomas. An approach to pathologic evaluation.

    abstract::Adenomatous polyps are common neoplastic lesions of the large intestine. The risk of carcinoma increases with polyp size. Small polyps are typically totally embedded for histologic examination, but no standard method for sampling large, grossly benign polyps has been established. We reviewed grossly noninvasive adenom...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/804U-LHU1-TJ00-UGAU

    authors: Euscher ED,Niemann TH,Lucas JG,Kurokawa AM,Frankel WL

    更新日期:2001-09-01 00:00:00

  • Fresh and citrated whole-blood specimens can produce different thromboelastography results in patients on extracorporeal membrane oxygenation.

    abstract:OBJECTIVES:To compare thromboelastography (TEG) tracings obtained from fresh and citrated whole-blood samples in patients on extracorporeal membrane oxygenation (ECMO) or after cardiopulmonary bypass and in healthy volunteers. METHODS:Samples of fresh and citrated whole blood were analyzed for 25 patients and 4 health...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPYIQ9JNNSEN4Q

    authors: Gilman EA,Koch CD,Santrach PJ,Schears GJ,Karon BS

    更新日期:2013-08-01 00:00:00

  • Hidden mastocytosis in acute myeloid leukemia with t(8;21)(q22;q22).

    abstract:OBJECTIVES:To assess the frequency of systemic mastocytosis (SM) in a large series of acute myeloid leukemia (AML) with t(8;21)(q22;q22). METHODS:We retrospectively characterized 40 bone marrow aspirate smears and biopsy specimens from patients with AML with t(8;21) for the presence of SM. Cases were assessed for mast...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP1Q0YSXEAHNKK

    authors: Johnson RC,Savage NM,Chiang T,Gotlib JR,Cherry AM,Arber DA,George TI

    更新日期:2013-10-01 00:00:00

  • Cardiac troponin T is elevated in asymptomatic patients with chronic renal failure.

    abstract::Patients with chronic renal failure (CRF) are at increased risk for myocardial events that are difficult to evaluate due to atypical symptoms and chronically elevated protein markers of cardiac damage. This study evaluated cardiac troponin T (cTnT), a sensitive marker of cardiac injury, in patients with CRF without my...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/106.1.118

    authors: Frankel WL,Herold DA,Ziegler TW,Fitzgerald RL

    更新日期:1996-07-01 00:00:00

  • Fibromatosis of the Breast: Diagnostic Accuracy of Core Needle Biopsy.

    abstract:Objectives:Fibromatosis of the breast is an uncommon neoplasm with potential for local recurrence. Treatment has traditionally been surgical excision with current trends toward conservative management. Given the option of observation after diagnosis by core needle biopsy (CNB), we sought to evaluate the accuracy of CNB...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/aqx065

    authors: Kuba MG,Lester SC,Giess CS,Bertagnolli MM,Wieczorek TJ,Brock JE

    更新日期:2017-09-01 00:00:00

  • Adamantinoma of the pretibial soft tissue. Clinicopathologic features, differential diagnosis, and possible relationship to intraosseous disease.

    abstract::The authors illustrate two neoplasms arising in the pretibial soft tissue that are histologically identical to adamantinoma of the long bones. The tumors developed in a 24-year-old woman and a 31-year-old man. Both were treated by local excision. Histologically, one tumor had a tubular pattern and one was composed of ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/83.1.108

    authors: Mills SE,Rosai J

    更新日期:1985-01-01 00:00:00

  • Sensitivity, specificity, and reproducibility of four measures of laboratory turnaround time.

    abstract::The authors studied the performance of four measures of laboratory turnaround time: the mean, median, 90th percentile, and proportion of tests reported within a predetermined cut-off interval (proportion of acceptable tests [PAT]). Measures were examined with the use of turnaround time data from 11,070 stat partial th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/91.4.452

    authors: Valenstein PN,Emancipator K

    更新日期:1989-04-01 00:00:00

  • JAK2 mutations across a spectrum of venous thrombosis cases.

    abstract::The JAK2(V617F)mutation is recurrent in polycythemia vera and essential thrombocythemia, which are myeloproliferative neoplasms frequently associated with arterial and venous thromboembolism. It has also been reported as a marker for occult myeloproliferative disorder (MPD) in patients with splanchnic venous thrombosi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCP7VO4HAIZYATP

    authors: Shetty S,Kulkarni B,Pai N,Mukundan P,Kasatkar P,Ghosh K

    更新日期:2010-07-01 00:00:00

  • Usefulness of a monoclonal ERG/FLI1 antibody for immunohistochemical discrimination of Ewing family tumors.

    abstract::Ewing family tumors (EFTs) and prostate carcinomas are characterized by rearrangement of ETS genes, most commonly FLI1 (EFTs) and ERG (prostate carcinomas). Previously, we characterized an antibody against ERG (EPR3864) for detecting ERG-rearranged prostate carcinoma. Because EPR3864 also cross-reacts with FLI1, we ev...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/AJCPN4L1BMRQPEIT

    authors: Tomlins SA,Palanisamy N,Brenner JC,Stall JN,Siddiqui J,Thomas DG,Lucas DR,Chinnaiyan AM,Kunju LP

    更新日期:2013-06-01 00:00:00

  • Theory of network planning for laboratory research and development.

    abstract::Critical Path Method (CPM) and Program Evaluation and Review Technique (PERT) are quantitative network planning strategies that facilitate management of projects with many interrelated activities. They determine the minimum time necessary to complete a project and identify which activities are critical to achieving th...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/79.3.353

    authors: Kost GJ

    更新日期:1983-03-01 00:00:00

  • Sensitivity and detection efficiency of the peroxidase antiperoxidase (PAP), avidin-biotin peroxidase complex (ABC), and peroxidase-labeled avidin-biotin (LAB) methods.

    abstract::The authors have examined the sensitivity and detection efficiency of the three peroxidase methods that currently have the widest application in diagnostic immunohistochemistry: the peroxidase-antiperoxidase (PAP), the avidin-biotin complex (ABC), and the labeled avidin-biotin (LAB) methods. Sensitivity was evaluated ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/92.1.62

    authors: Elias JM,Margiotta M,Gaborc D

    更新日期:1989-07-01 00:00:00

  • Transfusion-related acute lung injury: past, present, and future.

    abstract::Noncardiogenic pulmonary edema caused by transfusion has been observed for almost 60 years. Today, we know this entity as transfusion-related acute lung injury (TRALI). TRALI is an uncommon but potentially fatal adverse reaction to transfusion of plasma-containing blood components. It is typified by dyspnea, cough, hy...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章,评审

    doi:10.1309/D3F7BXH466AE3G0P

    authors: Cherry T,Steciuk M,Reddy VV,Marques MB

    更新日期:2008-02-01 00:00:00

  • CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms.

    abstract::We evaluated the immunohistochemical profile and specificity of CD138 reactivity in 238 specimens from hematopoietic and nonhematopoietic neoplasms. In 91 bone marrow biopsies, CD138 reactivity was observed for nonneoplastic plasma cells, neoplastic plasma cells in multiple myeloma cases (43/43), and the plasmacytic c...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/617D-WB5G-NFWX-HW4L

    authors: O'Connell FP,Pinkus JL,Pinkus GS

    更新日期:2004-02-01 00:00:00

  • Ischemia-modified albumin improves the usefulness of standard cardiac biomarkers for the diagnosis of myocardial ischemia in the emergency department setting.

    abstract::We studied the role of ischemia-modified albumin (IMA) with standard biomarkers (myoglobin, creatine kinase-MB [CK-MB], troponin I [TnI]) in assessment of 200 patients with suspected myocardial ischemia admitted to the emergency department. Every case was reviewed by a cardiologist. A clinical diagnosis of ischemia wa...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/4bctg5ucymqfwblr

    authors: Anwaruddin S,Januzzi JL Jr,Baggish AL,Lewandrowski EL,Lewandrowski KB

    更新日期:2005-01-01 00:00:00

  • Quantitation of hypertensive nephrosclerosis on an objective rational scale of measure in adults and children.

    abstract::The development of a precise, mathematical relationship between blood pressure and renal microvascular abnormalities would be highly desirable. Such a relationship would require that abnormalities be quantitative on a rational scale. The dominant abnormality in nephrosclerosis occurs in arcuate and cortical arteries o...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/85.3.312

    authors: Tracy RE,Mercante DE,Moncada A,Berenson G

    更新日期:1986-03-01 00:00:00

  • The evaluation of factor VIII binding activity of von Willebrand factor by means of an ELISA method: significance and practical implications.

    abstract::One of the functions of von Willebrand factor (vWF) is to serve as a carrier of clotting factor VIII (FVIII). Deficiency of this function results in the von Willebrand disease (vWD) variant type 2N, which resembles hemophilia A. We describe a new sandwich enzyme-linked immunosorbent assay (ELISA) to study the ability ...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/109.3.347

    authors: Casonato A,Pontara E,Zerbinati P,Zucchetto A,Girolami A

    更新日期:1998-03-01 00:00:00

  • Using fluorescence-based human androgen receptor gene assay to analyze the clonality of microdissected dendritic cell tumors.

    abstract::The clonality of dendritic cell proliferations arising in patients with previously diagnosed B-cell non-Hodgkin lymphoma has not been determined. The highly polymorphic human androgen receptor gene (HUMARA) can be used to assess the pattern of X-chromosome inactivation and, hence, the clonality of tumors in female pat...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/111.1.105

    authors: Wu CD,Wickert RS,Williamson JE,Sun NC,Brynes RK,Chan WC

    更新日期:1999-01-01 00:00:00

  • The effect of media and temperature on the storage of Chlamydia trachomatis.

    abstract::Four media, Eagle's minimal essential medium with 10% fetal bovine serum (MEM/FBS), tryptic soy broth (TSB), 2-SP, and 4-SP, were compared for their ability to maintain the viability of Chlamydia trachomatis at 4 degrees C, -20 degrees C, -70 degrees C, and -176 degrees C (liquid nitrogen) over a 1-week period. 2-SP m...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1093/ajcp/81.2.237

    authors: Aarnaes SL,Peterson EM,De La Maza LM

    更新日期:1984-02-01 00:00:00

  • Acetic acid-zinc-formalin: a safe alternative to B-5 fixative.

    abstract::B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, includi...

    journal_title:American journal of clinical pathology

    pub_type: 杂志文章

    doi:10.1309/29DA-CY9K-BHNW-4BG6

    authors: Bonds LA,Barnes P,Foucar K,Sever CE

    更新日期:2005-08-01 00:00:00