Abstract:
:Transplanted embryonic stem (ES) cells can be integrated into the retinas of adult mice as well-differentiated neuronal cells. However, the integrated ES cells also have a tumorigenic effect just because they have the ability for multipotential differentiation to various types of tissues. Thus, control of neoplastic potentials of ES cells is very important for the treatment of degenerative or injured diseases. Mouse ES cells carrying the sequence for the green fluorescent protein (GFP) gene were transplanted into adult mouse retinas by intravitreal injections 20 h after intravitreal N-methyl-d-aspartate (NMDA) administration. One week after the ES cell injection, folate antagonist methotrexate (MTX) was injected intravitreally. Eyes were retrieved 4 weeks after ES cell transplantation for histologic analyses. Conventional histological analysis was performed by hematoxylin and eosin staining with the use of paraffin-embedded sections. Neuronal differentiation and teratogenic potential of ES cells were demonstrated by immunohistochemistry. The proliferative activity of transplanted cells was detected by mitotic index, proliferating cell nuclear antigen index and AgNOR count. The incorporation of transplanted ES cells in MTX-treated and non-treated retinas at 4 weeks after transplantation was observed in 8/16 eyes (50%) and 8/16 eyes (50%), respectively. Transplanted ES cells in MTX-treated retina showed increased neuronal differentiation and decreased expression of teratogenic markers, compared with ES cells in non-treated retina. The proliferative activity of transplanted ES cells in MTX-treated retina was lower than that in non-treated retina. These results suggest that intravitreal MTX treatment following transplantation can induce neuronal differentiation in the transplanted ES cells and decrease their proliferative activity.
journal_name
Brain Resjournal_title
Brain researchauthors
Hara A,Niwa M,Kumada M,Aoki H,Kunisada T,Oyama T,Yamamoto T,Kozawa O,Mori Hdoi
10.1016/j.brainres.2006.02.079subject
Has Abstractpub_date
2006-04-26 00:00:00pages
33-42issue
1eissn
0006-8993issn
1872-6240pii
S0006-8993(06)00499-9journal_volume
1085pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Following extirpation of three quarters of the eye anlage of Xenopus laevis in stage 33/34, the remaining quadrants developed into small eyes of normal shape. Irrespective of the different portions of the eye anlage the quarter eyes originated from, the retinotectal projection was always confined to the rostrolateral ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(86)90089-1
更新日期:1986-09-01 00:00:00
abstract::Pentobarbital-anesthetized rats received electrical hindpaw stimulation every 10 s to elicit a maximal hindlimb withdrawal reflex. The integrated EMG response in the ipsilateral tibialis anterior was sampled by a computer which also controlled the timing of electrical stimuli applied to the brain. A suppression of the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91142-6
更新日期:1989-10-09 00:00:00
abstract::Previous studies employing systemic administration of the dopamine agonist apomorphine have shown that the dose response curves for apomorphine's effects on penile reflexes and seminal emission differ, suggesting that experimentally separable populations of dopamine receptors regulate these two responses. The present ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90328-4
更新日期:1989-10-23 00:00:00
abstract::Estrogen effects on tyrosine hydroxylase (TH), monoamine oxidase types A and B (MAO), and dopamine (DA) in microdissected regions of the hypothalamus, preoptic area and substantia nigra (SNR) of the female rat brain were investigated. Ovariectomized (OVX) young adult female rats were implanted with single silastic cap...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90730-y
更新日期:1990-02-26 00:00:00
abstract::Our previous study revealed that, during tonic muscle contraction, remarkable functional differences among intrinsic and extrinsic muscles were observed during two different grip movements, i.e., precision and power grips. To verify whether this evidence is true even under the phasic muscle contraction, magnetic stimu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02368-5
更新日期:2002-05-03 00:00:00
abstract::Bilateral removal of the cortical visual area in newborn cats produces degeneration of retinal ganglion cells. Measurements of over 4,000 cells and calculation of neuron densities from sample areas of retina in the adult show that the medium sized cell population in peripheral retina is reduced by 68%, whereas the pop...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90480-7
更新日期:1981-05-18 00:00:00
abstract::The relationship between placental antigen X-P2 (PAX)-immunoreactive glomeruli and intensely acetylcholinesterase-reactive (IAE) patchy regions was evaluated by comparison of neighboring cryostat sections of the rat olfactory bulb. Both groups of distribution show similar necklace patterns. Each IAE region consists of...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90440-x
更新日期:1993-07-30 00:00:00
abstract::Suppression of geniculate ponto-geniculo-occipital (PGO) waves was achieved in kittens by performing bilateral mesencephalic lesions of the PGO pathways. Sleep-wake parameters and the spontaneous unit activity in the lateral geniculate nucleus (LGN) were then chronically recorded during one month after the lesions. Wh...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90735-9
更新日期:1987-04-14 00:00:00
abstract::Synapses from Mauthner to giant fibers in the hatchetfish are chemically transmitting excitatory axo-axonic synapses located in the medulla. The synapses are 4--10 mum in diameter and easily identified for electron microscopy. Presynaptic vesicles are clustered near the contact regions and are round, clear and 40-60 n...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90002-5
更新日期:1975-11-14 00:00:00
abstract::Peripheral axotomy in neonatal rats induces neuronal death. We studied the anti-apoptotic protein Bcl-2 and cell death promoter Bax in spinal cord of neonatal rats after sciatic transection and treatment with melatonin, a neuroprotective substance. Pups were unilaterally axotomized at P2 and received melatonin (1 mg/k...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.07.021
更新日期:2006-09-27 00:00:00
abstract::Entopeduncular nucleus (EPN) cells which project to thalamic and non-thalamic sites were identified by antidromic discharge. Each population of cells, projecting to the ventral anterior nucleus of the thalamus (VA), centrum medianum (CM), the lateral habenula (LHB), and the pedunculopontine nucleus (PP), was distribut...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90993-9
更新日期:1978-11-17 00:00:00
abstract::Behavioral assessments of mouse models of neurodegenerative disorders are useful for investigating the molecular basis of the pathologies of the diseases. Here, we investigated the utility of a water-finding test using a video tracking system as a tool for evaluating cognitive deficits in Alzheimer's disease model mic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2012.10.066
更新日期:2013-01-23 00:00:00
abstract::Cerebellum is involved in the motion coordination and working memory, to which spike programming at Purkinje neurons are essential. The development of Purkinje neurons in the embryonic stage has been well studied. However, it is not clear about the maturation of their intrinsic property related to spike programming du...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.04.092
更新日期:2006-06-30 00:00:00
abstract::The properties of 5 beta-reductase, 5 alpha-reductase and aromatase, 3 testosterone metabolizing enzymes, were studied in the quail brain by an in vitro incubation technique. The results describe the changes in time of metabolite production and the effects of temperature, enzyme and cofactor concentrations. The appare...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91118-1
更新日期:1984-07-02 00:00:00
abstract::The patterns of preganglionic projections in the avian sympathetic system are highly specific. Preganglionic axons arising from the T1 spinal segment, for example, project predominantly in the rostral direction, whereas T4 preganglionic axons project predominantly in the caudal direction. Moreover, each ganglion recei...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90149-0
更新日期:1987-03-01 00:00:00
abstract::Myelin is probably one of the most fascinating and innovative biological acquisition: a glia plasma membrane tightly wrapped around an axon and insulating it. Chondrichthyans (cartilaginous fishes) form a large group of vertebrates, and they are among oldest extant jawed vertebrate lineage. It has been known from stud...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2016.01.013
更新日期:2016-06-15 00:00:00
abstract::The preferential loss of GABAergic neurons in neocortex slab cultures after exposure to hypoxia was reinvestigated and confirmed using an improved morphometric analysis based on stereological principles. This preferential cell loss could be attenuated by treating the hypoxic slab cultures with the N-methyl-D-aspartate...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91031-7
更新日期:1989-10-30 00:00:00
abstract::Attempts were made to determine the cells of origin of the spinal projections to the PAG using retrograde axoplasmic transport techniques of HRP and fluorescent markers. Following injections limited to the lateroventral subnucleus of the PAG, labels were transported to the contralateral laminae I, III, V, VII and X pr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)91127-7
更新日期:1983-03-28 00:00:00
abstract::Corticofugal regulation of excitatory and inhibitory frequency tuning curves (FTCs) of neurons in the central nucleus of bat inferior colliculus (ICc) was studied by electrical stimulation of the primary auditory cortex (AC stimulation) under free field stimulation conditions using a two-tone inhibition paradigm. AC s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01786-2
更新日期:1999-09-11 00:00:00
abstract::To see if activation of central alpha 1-adrenergic receptors can cause facilitation of lordosis in rats, the behavioral effects of centrally administered alpha 1-agonists, methoxamine (MA) and phenylephrine (PhE), and related agents were studied. In ovariectomized rats treated with estrogen, infusion of MA, PhE, or a ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91581-x
更新日期:1992-08-21 00:00:00
abstract::The distributions of serotonin and catecholamines were examined within the central ganglia of juvenile Achatina through the histological localization of serotonin-like immunoreactivity and of glyoxylic acid-induced fluorescence. Somata containing these amines were widely distributed throughout all central ganglia exce...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90427-1
更新日期:1988-09-13 00:00:00
abstract::The distribution of retinal ganglion cells (RGCs) providing input to the thalamofugal visual system in the pigeon was studied with an anatomical transneuronal transport technique using the fluorescent dye rhodamine beta-isothiocyanate (RITC). Unilateral injections of RITC made into the telencephalic visual Wulst resul...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.04.091
更新日期:2006-07-07 00:00:00
abstract::Activated monocytes/macrophages are known to release toxic materials. Identification of these materials is important for developing more effective treatments for inflammatory disorders where self attack occurs. We stimulated human monocytes and THP-1 cells with LPS/IFNγ and measured the toxic effects of their conditio...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.05.021
更新日期:2011-07-11 00:00:00
abstract::The effects of caffeine ingestion and exposure to bright light, both separately and in combination, on salivary melatonin and tympanic temperature were assessed in humans. Four treatments during a 45.5 h sleep deprivation period were compared: Dim Light-Placebo, Dim Light-Caffeine, Bright Light-Placebo and Bright-Ligh...
journal_title:Brain research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0006-8993(96)01268-1
更新日期:1997-01-30 00:00:00
abstract::Inflammation is an important factor for hypoxia-ischemia (HI) brain injury. Interleukin (IL)-18 is a proinflammatory cytokine which may be a contributor to injury in the immature brain after HI. To investigate the effects of post-HI hypothermia on IL-18 in the developing brain, 7-day-old rats were subjected to left ca...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.08.121
更新日期:2006-11-22 00:00:00
abstract::In seven female alpha-chloralose-anesthetized cats, 52 lateral thalamic neurons were tested with noxious distension of the urinary bladder, the distal colon and the lower esophagus. In addition, the neurons were characterized with innocuous and noxious mechanical stimulation of the skin and deep structures. Of the 52 ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02180-0
更新日期:1999-12-18 00:00:00
abstract::Pregnenolone sulfate (PS) is an endogenous neurosteroid known to antagonize GABA(A) receptor-mediated inhibitory responses and potentiate NMDA receptor-mediated excitatory responses in vitro. To assess the actions of the steroid as a modulator of seizure susceptibility in vivo, PS (30-300 nmol) was administered intrac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01287-1
更新日期:1999-06-12 00:00:00
abstract::Production of inflammatory cytokines, including tumor necrosis factor (TNF), interleukin-1 (IL-1) and interleukin-6 (IL-6), in the brain is increased in various diseases. To investigate the relationships between the effect of overproduction of IL-6 in the brain on central and peripheral production of TNF, IL-1 beta an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)00865-7
更新日期:1996-11-18 00:00:00
abstract::We describe a method to produce frog olfactory epithelium with a population of developmentally synchronized receptor cells suitable for electrophysiological studies. The epithelium is ablated with ZnSO4 and allowed to regenerate for 10 days. Generation of new cells is then blocked by continuous treatment with hydroxyu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90122-2
更新日期:1987-02-01 00:00:00
abstract::We recently reported raphe neurones which frequently fired spikes in short bursts. However, the action potentials were broad and the neurones fired in a slow and regular pattern, suggesting they were an unusual type of 5-hydroxytryptamine (5-HT) neurone. In the present study, we investigated whether these putative bur...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)00869-4
更新日期:1996-11-18 00:00:00