Abstract:
:The aim of this study was to evaluate the automated urine test strip analyzer Aution Max AX 4280 which uses strips able to measure 12 urinary parameters. For precision study, we considered glucose, protein, blood, leukocytes, nitrite, and pH and the four first were compared with usual methods: chemical measurement or microscopic examination. Reproducibility of the semi quantitative results was determined as the proportion of results falling into the same concentration range. Within-run reproducibility assessed using urine specimens were between 83% and 96%. Between day on quality controlled materials, it was higher (96%), and leukocytes were shared between two classes 250 and 500 cells/microL. For glucose and proteins, linearity was good and comparison with quantitative methods yielded high correlation. The false negative fraction was low and there was no false positive results. For blood, fraction of false positive was 7.5% for 0.3 mg/L of hemoglobin compared with microscopic analysis and false negative fraction was 1.3%. For leukocytes, false positive fraction was 4%, false negative one was 7.8%. These results, values for sensitivity, specificity, predictive positive value, predictive negative value permitted to determine the cut-off values to realize second levels analysis: proteins are measured from 0.30 g/L, glucose from 2.8 mmol/L, microscopic urinalysis was performed from hemoglobin more than 0.3 mg/L and leukocytes more than 25 cells/microL.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Steinmetz J,Henny J,Gueguen Rsubject
Has Abstractpub_date
2005-11-01 00:00:00pages
619-26issue
6eissn
0003-3898issn
1950-6112journal_volume
63pub_type
临床试验,杂志文章abstract::Three methods (rapid bioassay, enzyme immunoassay and fluoroimmunoassay) for the determination of amikacin level in serum are compared. The statistical analysis of the results show correlation coefficients equal to 0.967 (EMIT versus FIA), 0.970 (bioassay versus FIA) and 0.983 (bioassay versus EMIT). The three methods...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::We have compared serum galactosyltransferase activity in pregnant women and in newborn children. Subjects in this study were 75 women at different stages of pregnancy and 60 newborns, including premature infants. Activity ratios in pregnant women are based on the period of gestation, expressed in weeks of amenorrhea. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The purpose of this study is to find, besides the determination of lactate in blood, a suitable and specific indicator of brain damage in newborn. Accordingly, we studied the total creatine kinase (CK) activity, by the method of Rosalki, and its subunit isoenzymes BB (CK BB) by fluorescence following electrophoresis o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::An automated kinetic method to determine creatinine in plasma with Jaffé reaction (alkaline picrate reagent) using a Gemsaec analyser is studied in order to argue the precision and the accuracy. The analysis of the reaction rate of a primar standard solution and of different plasmas suggests that a rapid measure is re...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::A previous multicentric study set up by the Société française de biologie clinique has emphasized the usefulness of a standardized procedure for the determination by high performance liquid chromatography of alpha-tocopherol in serum or plasma. In our study, we have tested every step of the different published procedu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
abstract::The specificity of six peroxidase-labelled anti-albumin monoclonal antibodies was studied by allowing them to react with albumin-derived fragments of known structure comprising the whole albumin molecule. The epitopes corresponding to these antibodies were located on defined regions of the albumin molecule extending f...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1990-01-01 00:00:00
abstract::We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Haloperidol is extracted with hexane-isoamyl alcohol in the presence of two internal standards, chlorohaloperidol and spiroperidol. For chromatographic separation, a C18 reversed-phase column and a mobile phase of acetonitrile/methanol/phosphate buffer are used. Detection is by UV absorption spectrophotometry at 245 n...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. This new category of lymphoma "double hit" (DH) is part of a new entity of the WHO classification 2008: « Unclassifiable B lymphoma with features intermediate between diffuse large cell B lymphoma and Burki...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2016.1189
更新日期:2016-12-01 00:00:00
abstract::Acute arsenic poisoning is a rare cause of suicide attempt. It causes a multiple organs failure caused by cardiogenic shock. We report the case of a patient admitted twelve hours after an ingestion of trioxide arsenic having survived thanks to a premature treatment. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.1012
更新日期:2014-11-01 00:00:00
abstract::Monoclonal antibodies have been raised against porcine LH receptor and allowed to clone the corresponding messenger RNA from testicular cells. Cross hybridisation with the LH receptor clone allowed to isolate a clone corresponding to the human TSH receptor from thyroids. The structure of both receptors have been deter...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Eighteen paraplegics with or without neurogenic osteo-arthropathy (POAN) were evaluated for variations in their urinary excretion levels of total (T), and free (L) hydroxyproline and its non-dialysable (ND) and dialysable (D) peptide fractions. Increases in urinary hydroxyproline, moderate rises in free hydroxyproline...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Total plasma homocysteine emerged in the past few years as an independent risk factor for cardiovascular diseases. This test is now currently prescribed for the diagnosis of unexplained thrombosis in young adults or recurrent thrombosis in patients with arteriopathy. This sulphured amino-acid is an important intermedi...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::The authors describe a method of fractionation of urinary 17-oxosteroids after purification of the urinary extract by Girard's T reagent. After solvolysis, enzyme hydrolysis and purification by Girard's T reagent, the 17-oxosteroids were estimated in the form of trimethyl silyl ethers by gas liquid chromatographye on ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Type 2 diabetes (T2DM) associated with non-alcoholic fatty liver disease (NAFLD) increases cardiovascular risk. Complex and subtle connections are established between hepatic dysfunction and adipose tissue hyperactivity. This relationship is mediated by insulin resistance, dyslipidemia and inflammation. Recently incre...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2019.1436
更新日期:2019-06-01 00:00:00
abstract::Salivary cortisol was determined with four commercially available immunoassays: one enzyme-immunoassay (Cortisol Biotrol), two fluoro-immunoassays (TDX, Abbott; Stratus, Baxter) and one radioimmunoassay (Coat-A-Count, Behring). In order to improve the sensitivity of these immunoassays, it was necessary to increase sam...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::The stimulation regimens and the results of ovulation induction in anovulatory patients and in patients suffering from a Luteinized Unruptured Follicle (LUF) syndrome are discussed as well as the findings concerning superovulation in IVF cycles. The percentage of multiple pregnancies (less than or equal to 20 p. cent)...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::The most generalized methods in enzymology are based on the quantitative assay of compounds, substrate or coenzyme, by spectrophotometry without any separation. Such a method is ruled out if the colorimetric reaction is not specific of the compound. In liver enzymology, aside the classical metabolic pathways, such ass...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::A group of genetic disorders of the red cell, appearing as congenital haemolytic anaemias, were first described on morphological bases, due mainly to the presence of stomatocytes. It turned out, however, that the alterations of the shape could widely vary, and even be absent, thereby questionning the classification on...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1999-07-01 00:00:00
abstract::We describe the case of a serological reactivation in a Toxoplasma-seropositive subject, following a cardiac transplantation transmitting cysts contained in the myocardial tissue. In a context of acute graft rejection, primary chemoprophylaxis enables to avoid onset of opportunistic toxoplasmosis, emerging with immuno...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2012.0706
更新日期:2012-05-01 00:00:00
abstract::There is much variability in the manner individuals respond to drugs, such that the management of some drugs is problematic. In France, the incidence of hospital admissions related to adverse drug reactions is estimated to be 3.2 %, at an annual cost of over 300 millions euros. Genetic factors affecting the pharmacoki...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2004-09-01 00:00:00
abstract::Twenty four strains of coagulase-negative staphylococci from clinically significant blood cultures were separated in four groups on the basis of oxacillin susceptibility and phenotype of resistance to aminoglycoside antibiotics. Susceptibility testing included disk diffusion testing, M.I.C. and M.B.C. determinations a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Anemia is the most common pathology encountered in hematology. Etiologies are numerous, so it is important to adopt a rigorous approach. Complementary examinations must be specific to the clinical situation in order to determine the mechanisms on the one hand and decide the therapeutic management on the other. We repo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2011.0584
更新日期:2011-05-01 00:00:00
abstract::Peripheral gangrene with disseminated intravascular coagulation (DIC) during severe Plasmodium falciparum malaria has already been described but is unfrequent. We report here the case of a 62-year-old man admitted in the intensive care unit of our hospital for severe Plasmodium falciparum malaria with disseminated int...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2009.0367
更新日期:2009-09-01 00:00:00
abstract::According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0891
更新日期:2013-09-01 00:00:00
abstract::Acquired hemophilia A (AHA) is a rare and potentially severe bleeding disorder caused by circulating autoantibodies directed against factor (F) VIII. Apart from idiopathic cases, AHA is associated with autoimmune diseases, cancers, use of medications, pregnancy and the post-partum period. We report the case of a 78-ye...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2018.1405
更新日期:2019-04-01 00:00:00
abstract::We present the distribution of cytochrome P450, the terminal oxidase of the microsomal drug-metabolizing system among the different species, organs, and its subcellular localization. Physicochemical properties, and more specially spectral properties, of this hemoprotein are reviewed. We also described the two microsom...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1975-01-01 00:00:00
abstract::Attention has been drawn in recent years to the diagnostic interest offered by increases in serum gamma GT in the detection of liver metastases and various cancers in man. Although this analysis is not specific and presents a few false positive and negative results, it seems however profitable to include it either in ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1981-01-01 00:00:00
abstract::HTLV genomic and antigenic features, replication way as well as associated pathology are recalled herein. The epidemiologic angle and the different transmission ways are also related. HTLV infection diagnostic implements are detailed: screening and specially confirmatory tests are brought to light with the help of con...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1998-09-01 00:00:00
abstract::The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00