Abstract：
:The purpose of this study is to find, besides the determination of lactate in blood, a suitable and specific indicator of brain damage in newborn. Accordingly, we studied the total creatine kinase (CK) activity, by the method of Rosalki, and its subunit isoenzymes BB (CK BB) by fluorescence following electrophoresis on cellulose acetate. A first group (thirty newborns) without any disease represents our taest group. We havae found that the normal ranges for CK total were elevated (about 7 200 nkat/l). Electrophoresis of sera from these patients showed in addition to the normally migration isoenzyme (CK BB) was present in very small amounts, no sufficient for quantification. A second group was constituted to thirty ill newborns, with perinatal brain damage. In twenty-eight to thirty children, we find a significant correlation between the level of CK BB and brain insult. But, we have showed that it was necessary to take in consideration the time passed between the hypoxic insult and the blood puncture. Otherwise, we showed by immuno inhibition with specific antibody and by chromatography with gradient elution that identification of CK BB by electrophoresis cannot be misinterpreted. Furthermore, we demonstrated by this method, that position of CK 1 BB in blood is exactly the same that an purified human extract. This study concluded that a high level CK 1 BB in blood of newborn infants with perinatal brain damage has an accurately diagnosis value, if the blood puncture is done immediately during the severe CNS damage.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Chopin N,Lavaud J,Voyer M,Bally M,Cloup Msubject
Has Abstractpub_date
1980-01-01 00:00:00pages
345-50issue
6eissn
0003-3898issn
1950-6112journal_volume
38pub_type
杂志文章abstract：:Micro- and nanospheres are tightly associated with the development of flow cytometry. They are indispensable tools to optimize diffraction and fluorescence signals as well as for fluorescence calibration and cellular purification (magnetic micro- and nanospheres). They are also usefull to evaluate phagocytosis and to ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent enzyme deficiency. It is a sex-linked genetic disease concerning mostly african, mediterranean and far-eastern populations. The main clinical expression is a hemolytic anemia which can be acute or chronic. During the neonatal period the disease m...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：:The erythrocyte bilirubin value is of great interest in neonate pathology. Its sudden rising reflects the overbinding capacity to plasmatic albumin. A colorimetric, simple and accurate micromethod with 2-hydrazino-benzothiazol as reagent leads to its quantitative determination. Hemolysis interference is removed. The a...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:In the present investigation, the immunoglobulins G of human sera were studied after isoelectrofocusing using polyacrylamide flat bed gel. The heterogeneity of immunoglobulins was demonstrated in normal serum as well as in pathological situations. Isoelectrofocusing study was performed using the total serum or the imm...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Cystatin C is a low molecular weight-protein, which may replace creatinine for the evaluation of renal function, particularly in the clinical settings where the relationship between creatinine production and muscular mass impairs the clinical performance of creatinine. This paper intends to summarize the current knowl...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2008.0227
更新日期：2008-05-01 00:00:00
abstract：:Human hair analysis is now recognized for evaluating someone exposure to xenobiotics: drugs of abuse, pharmaceuticals and polluants. This paper describes analytical methods than can be used by biologists. For drugs of abuse after decontamination, hydrolysis of hair, selective extraction and derivatization, determinati...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1997-09-01 00:00:00
abstract：:Determination of sex-hormone-binding-globulin was performed by three methods: binding, electroimmunodiffusion and DELFIA methods. Results of normal and pathological values correlated well with the three techniques. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Heterogeneous immunoassays are very sensitive and only limited in terms of performance by non specific binding. They require separation of free from bound fractions and concomitant use of a solid phase coated with an immunoreactive component (i.e. immunosorbent). The improvement of these key immunosorbents is crucial ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Macroprolactin is a complex of prolactin with immunoglobulins (IgG) that has limited or no biological activity in vivo. Immunoassays for prolactin have variable reactivity with macroprolactin. Therefore the presence of macroprolactin should be considered in the differential diagnosis of hyperprolactinemia. We compared...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:The performance of the HemoCue apparatus in the diagnosis of anaemia in pregnancy was evaluated using the HemoCue Hb 301 and the HemoCue Hb 201+ apparatus. The HemoCue Hb 201 + was considered as the reference method in this study. Two hundred and thirty six pregnant women attending the University Teaching Hospital in ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2008.0195
更新日期：2008-01-01 00:00:00
abstract：:Haemochromatosis is the most common genetic disease in individuals of Northern European origin. This disorder of iron metabolism, for which the molecular basis remains poorly understood, is characterized by an excessive absorption of dietary iron through the duodenal mucosa. Progressive iron loading of parenchymal org...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1997-05-01 00:00:00
abstract：:Salmonelloses are becoming more frequented. To date, very few cases of aneurisms or vascular grafts infections have been described. We report the rare case of an extradigestive manifestation, due to Salmonella enteritidis affecting a patient with Behçet disease. The biological diagnosis is based on microorganism isola...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2010.0405
更新日期：2010-01-01 00:00:00
abstract：:Twenty-two amino acids are measured in cerebrospinal fluid of fourty patients with senile dementia of the Alzheimer type (SDAT) and of ten age matched healthy control subjects. There is no significant difference between these two groups. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Using factorial design, the authors evaluate the factors which influence in vitro, the anti-fungal activity of ketoconazole against a strain of Candida albicans (CBS 562). With the four mediums most frequently used, (Sabouraud, Casitone, MEM, YNB) they precise the results concerning inoculum, pH, incubation temperatur...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Acquired hemophilia A (AHA) is a rare and potentially severe bleeding disorder caused by circulating autoantibodies directed against factor (F) VIII. Apart from idiopathic cases, AHA is associated with autoimmune diseases, cancers, use of medications, pregnancy and the post-partum period. We report the case of a 78-ye...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2018.1405
更新日期：2019-04-01 00:00:00
abstract：:In this review, after a brief summary of the spermatogenesis process, we present some genetic causes of male infertility at chromosome and gene level, known at present. Even though knowledge has greatly increased, in 15% of cases, we still do not know the infertility mechanism. The genotype-phenotype relationship rema...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:The authors describe an immunoassay technique of serum progesterone using a chemiluminescent tracer (LIA): progesterone aminobutylethylisoluminol 11-a-hemisuccinate (P-ABEI). The intrinsic characteristics of this technique were studied. The progesterone concentrations of 62 serum samples were simultaneously titrated u...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Anti native DNA antibodies (anti nDNA Ab), which are a highly specific feature of systemic lupus erythematosus (SLE) were measured by 3 methods: an enzyme linked immunosorbent assay (ELISA), an indirect immunofluorescence test on Crithidia luciliae (IFCL) and the Farr assay (reference test). 114 sera from patients wit...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Various coagulation abnormalities were reported in HIV-infected patients. Cases of severe thrombocytopenia were first observed in contaminated homosexual males, as well as prolonged APTT due to the presence of lupus-like anticoagulant with a frequency in the range 8 to 70% of the studied patients. Even if lupus antico...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1998-03-01 00:00:00
abstract：:Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：2002-11-01 00:00:00
abstract：:Diosmin (DI) 3'5,7-trihydroxy-4'-methoxy flavone rutinoside is a member of the flavonoid family, some of whom have antioxidant or free radical scavenger properties. Paw oedema induced by doxorubicin in rats is reduced by DI as observed by SAUVAIRE et al (1989). This suggests a free radical scavenger activity for DI. I...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:We evaluated a rapid and semi-quantitative C-Reactive Protein test on whole blood, the Actim CRP (Fumouze). Based on immuno-chromatography technology, this test ranked the blood sample in four groups: < 10 mg/L, 10-40 mg/L, 40-80 mg/L and > 80 mg/L. This evaluation finds an excellent repeatability, the absence of hook...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Direct and indirect ion selective electrodes (ISEs) are two methods commonly used in biochemistry laboratories in order to measure the electrolytes such as sodium. In the clinical practice, it's the sodium concentration in plasma water -measured by direct ISE- which is important to consider as it is responsible of wat...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2015.1046
更新日期：2015-05-01 00:00:00
abstract：:The full blood count (FBC) is the most prescribed laboratory test in France. Due to the lack of data, there is a great variability in reference values of the FBC, between medical laboratories. The aim of this work was to provide normal reference values for FBC in adults. These normal values were defined in a populatio...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2014.0979
更新日期：2014-09-01 00:00:00
abstract：:The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Increased level of C reactive protein in the serum of neonates indicates the presence of infection, whereas the classic signs (hyperthermia, neutrophilia...) may often fail. So the determination of C reactive protein is useful and has to be rapidly performed on a small quantity of blood. The authors evaluate a fluores...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The development of enzymo-immunological technics and the synthesis of efficacious immuno-adsorbants has given rise to several immuno-enzymometric methods of which one, ELISA (enzyme-linked immunosorbent assay) has proved particularly practical and sensitive for the estimation of antibodies. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1978-01-01 00:00:00
abstract：:Therapeutic monitoring of patients participating in methadone maintenance programmes requires the qualitative or quantitative determination of methadone in urine before and during treatment. Generally immunoassay techniques are used for this determination, but more and more often measurement of plasma or serum levels ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:The aim of this study was to evaluate the automated urine test strip analyzer Aution Max AX 4280 which uses strips able to measure 12 urinary parameters. For precision study, we considered glucose, protein, blood, leukocytes, nitrite, and pH and the four first were compared with usual methods: chemical measurement or ...
journal_title：Annales de biologie clinique
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-11-01 00:00:00
