[Contribution of immunoenzymatic technics to the assay of anti-native DNA antibodies in the biological diagnosis of lupus erythematosus disseminatus].

abstract：:The authors propose a technique of labelling of leukocytes based on the phagocytic power of these cells with regard to sulphide particles in the colloidal state labelled with 90mmTc. These labelled leukocytes maintain their normal function and can be detected in the inflammatory foci created in experimental animals in...

journal_title：Annales de biologie clinique

authors： Auvergnat JC,Simon J,Le Net R,Guiraud R,Armengaud M

更新日期：1975-01-01 00:00:00

abstract：:In assisted reproductive technology (ART), serum levels of several key hormones are used to evaluate ovarian follicular reserve and to monitor gonadotropin-stimulated follicle growth. Currently, serum estradiol, FSH, LH and inhibin B levels are examined and combined at the beginning of the menstrual cycle to evaluate ...

journal_title：Annales de biologie clinique

authors： Taieb J,Benattar C,Poüs C

更新日期：2003-09-01 00:00:00

abstract：:Second generation hepatitis C virus (HCV) Elisas are currently in use in Europe and have been submitted for approval in the United States. These new assays contain additional antigens from the putative nucleocapsid and NS-3 regions of the HCV genome in addition to the c100-3 antigen present in first generation Elisas....

journal_title：Annales de biologie clinique

authors： Polito AJ,DiNello RK,Quan S,Andrews W,Rose J,Lee F,Nelles M,Lee S

更新日期：1992-01-01 00:00:00

abstract：:The authors describe a method of simultaneous estimation of phenobarbitone, mephenytoin and diphenylhydantoin in the blood. After extraction with ether, the methylation of these substances by trimethylanilinium hydroxide permits their rapid separation by gas chromatography. Heptabarbitone is used as internal standard ...

journal_title：Annales de biologie clinique

authors： Maitrot B,Hue G,Canonne MA,Rocher A,Landriau N

更新日期：1976-01-01 00:00:00

abstract：:Determination of plasma delta-aminolaevulinic acid levels is of interest for the diagnosis of disorders of heme biosynthesis. We describe a reliable method for the determination of this parameter, based on a modification of the original method of Mauzerall and Granick. Analytical criteria are defined and the reference...

journal_title：Annales de biologie clinique

authors： Goujon R,Philibert E,Bernard P,Normand J,Boucherat M

更新日期：1992-01-01 00:00:00

abstract：:Activation of plasminogen by tissue-type plasminogen activator (tpA) is potentiated by fibrin. We have demonstrated the role of fibrin polymerization in the potentiating effect of tpA-induced fibrinolysis. Therefore a pathogenic mechanism of thrombotic disorder may be related to an abnormal fibrin polymerization: the ...

journal_title：Annales de biologie clinique

authors： Soria C,Soria J,Mirshahi M,Desvignes P,Bonnet P,Caen JP

更新日期：1987-01-01 00:00:00

abstract：:Urinary protein electrophoresis analysis (UPE) is an essential investigation for the study of abnormal proteins in urines. The interpretation of this analysis must be comprehensive and relevant. Indeed, UPE is often requested by clinicians and may have an important impact in patient's management. This paper presents t...

journal_title：Annales de biologie clinique

authors： Nel I,Thiolières JM,Ghillani-Dalbin P,Jacquiaud C,Piéroni L

更新日期：2017-02-01 00:00:00

abstract：:The aim of the present work was to assess the genetic and antigenic variability in the VP1 region of type 3 echviruses (E-3), an enterovirus serotype associated to meningitis, neuro-muscular diseases and type 1 diabetes in human. Forty-six VP1 sequences of E-3, among which 9 were isolated in tunisian infants, were inc...

journal_title：Annales de biologie clinique

authors： Farès W,Rezig D,Yahia AB,Touzi H,Meddeb Z,Triki H

更新日期：2012-03-01 00:00:00

abstract：:The presence of the R506Q mutation of the factor V gene is associated with an increased risk of thromboembolism, particularly during pregnancy. Recently, its involvement in the development of obstetrical complications, such as preeclampsia and fetal losses, has been evoked. The resulting factor VQ506 (factor V Leiden)...

journal_title：Annales de biologie clinique

authors： Magdelaine A,Soubrier F,Berkane N,Uzan S,Verdy E

更新日期：1998-07-01 00:00:00

abstract：:Myelodysplastic syndromes (MDS) are clonal disorders of pluripotent hematopoietic stem cells. MDS occur predominantly over the age of 60 years. The diagnosis of MDS is based on the examination of both blood films and bone marrow aspirate. Diseases such as vitamin B12 and/or folate deficiency, or cytotoxic therapy lead...

journal_title：Annales de biologie clinique

authors： Garandeau C,Pautas E,Andreux M,Andreux J,Gaussem P,Siguret V

更新日期：2000-07-01 00:00:00

abstract：:Three methods (rapid bioassay, enzyme immunoassay and fluoroimmunoassay) for the determination of amikacin level in serum are compared. The statistical analysis of the results show correlation coefficients equal to 0.967 (EMIT versus FIA), 0.970 (bioassay versus FIA) and 0.983 (bioassay versus EMIT). The three methods...

journal_title：Annales de biologie clinique

authors： Damien JM,Courcol RJ,Houdret N,Lhermitte M,Martin GR

更新日期：1984-01-01 00:00:00

abstract：:Panton-Valentine leukocidin-producing (PVL) Staphylococcus aureus is responsible for a highly lethal necrotizing pneumonia, which occurs predominantly in young immunocompetent patients. Hemoptysis and leucopenia often occur but are not always present. Detection of PVL gene on S. aureus strains responsible for pneumoni...

journal_title：Annales de biologie clinique

authors： Dubrous P,Cuguillère A,Gendrot A,Koeck JL

更新日期：2007-05-01 00:00:00

abstract：:Free and total tryptophan is estimated in the plasma by a spectrofluorimetric method using an amplification reaction and fluorescence by transformation of tryptophan into norharman using formalin and perhydrol (fluorescence is amplified about 15 times). Free tryptophan in the plasma is separated by ultrafiltration und...

journal_title：Annales de biologie clinique

authors： Escande C,Bousquet B,Dreux C

更新日期：1977-01-01 00:00:00

abstract：:Premature rupture of the membranes (PROM) is a frequent event affecting 3% of pregnancies. PROM causes 30% of premature deliveries and 20% of perinatal mortality. The diagnosis relies mainly on the clinical visualization of the amniotic fluid flow in the vagina. If not, clinicians can use bedside tests detecting eithe...

journal_title：Annales de biologie clinique

authors： Julien M,N'guema L,Bouzerara A,Toro B,Lecarpentier E,Guibourdenche J

更新日期：2018-06-01 00:00:00

abstract：:Hormonal production of the thyroid gland is constituted of thyroxine or T4 (80%) and triiodothyronine or T3 (20%). In the circulation, whole T4 originates from thyroid secretion but most of T3 (80%) is produced extrathyroidally from T4 deiodination. Conversion of T4 to T3 may be influenced by various conditions and ci...

journal_title：Annales de biologie clinique

authors： Sapin R,Schlienger JL

更新日期：2003-07-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent enzyme deficiency. It is a sex-linked genetic disease concerning mostly african, mediterranean and far-eastern populations. The main clinical expression is a hemolytic anemia which can be acute or chronic. During the neonatal period the disease m...

journal_title：Annales de biologie clinique

authors： Kaddari F,Sawadogo M,Sancho J,Lelong M,Jaby D,Paulin C,Nkana K,Cailliez M

更新日期：2004-07-01 00:00:00

abstract：:According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecu...

journal_title：Annales de biologie clinique

authors： Harlé A,Lion M,Husson M,Dubois C,Merlin JL

更新日期：2013-09-01 00:00:00

abstract：:The aim of this study is to present an evaluation of HbA(1c) Assay on Architect CI8200 (Abbott Diagnostic). The measurement includes Hb assay by colorimetry and HbA(1c) by immunoturbidimetry. The percentage of HbA(1c) is the report HbA(1c)/complete Hb with a conversion coefficient. Repetability (n = 30; CV: 1.15-1.91%...

journal_title：Annales de biologie clinique

authors： Beaune G,Ducruet J,Jund J,Favre S

更新日期：2009-01-01 00:00:00

abstract：:Anemia is the most common pathology encountered in hematology. Etiologies are numerous, so it is important to adopt a rigorous approach. Complementary examinations must be specific to the clinical situation in order to determine the mechanisms on the one hand and decide the therapeutic management on the other. We repo...

journal_title：Annales de biologie clinique

authors： Roche C,Roche NC,Thefenne H,Saidi R,De Pina JJ,Molinier S,Oliver M

更新日期：2011-05-01 00:00:00

abstract：:Biermer's disease is an autoimmune atrophic gastritis of the fundus predominantly responsible for a malabsorption of vitamin B12. Despite its association with several autoimmune disorders, few observations have reported an association with autoimmune hemolytic anemia (AIHA). We report a case of Biermer's disease assoc...

journal_title：Annales de biologie clinique

authors： Nafil H,Tazi I,Mahmal L

更新日期：2012-07-01 00:00:00

abstract：:Human embryonic stem cells (hESC) are obtained from the inner cell mass from the early embryo at blastocyste stage. Derived in cell lines for the first time in 1998, they can be maintained in culture in an undifferentiated state indefinitely under certain conditions. Two essential properties characterize hESC: pluripo...

journal_title：Annales de biologie clinique

authors： Tondeur S,Assou S,Nadal L,Hamamah S,De Vos J

更新日期：2008-05-01 00:00:00

abstract：:The recent development of technics of specific fixation using radio isotope-labelled tracer elements permits the demonstration of various types of receptors at the level of the lymphocyte membrane. Thus a new route is open in the understanding of the biochemical mechanisms controlling the physiology of the lymphocyte ...

journal_title：Annales de biologie clinique

authors： Arrang JM,Bidart JM

更新日期：1980-01-01 00:00:00

abstract：:The recommended conservative treatment of hyperoxaluria is mainly based on hyperhydration and ingestion of inhibitors of crystallization. In accordance with this context, the aim of this study was to determine the in vitro effect of Hordeum vulgare on calcium oxalate crystallization oxalo-dependent. The crystallizatio...

journal_title：Annales de biologie clinique

authors： Djaroud S,Harrache D,Amar A

更新日期：2012-11-01 00:00:00

abstract：:Elderly patients are at high risk of over-anticoagulation and of haemorrhagic risk when on warfarin, especially during treatment induction. In Charles Foix Hospital, a 800-bed geriatric hospital, we specifically developed for in-patients older than 70 years (target INR 2.5) a simple low-dose warfarin induction regimen...

journal_title：Annales de biologie clinique

authors： Siguret V,N'Guyen A,Harboun M,Chevallier A,Pautas E,Gouin-Thibault I,Berigaud S

更新日期：2006-05-01 00:00:00

abstract：:Sixty-three strains of Pseudomonas paucimobilis, a Gram-negative, lemon yellow pigmented, glucose nonfermenting bacillus are phenotypically compared to the type strain of this new species (ATCC 29837). The morphological, cultural, biochemical (ONPG-test, oxidase, aesculin positive) and nutritional (assimilation of man...

journal_title：Annales de biologie clinique

authors： Richard C

更新日期：1986-01-01 00:00:00

abstract：:The articular cartilage is frequently destroyed in rheumatic diseases. Among the various responsible factors, enzymes and especially collagenases play a leading role. Their action explains the collagen degradation linked with cartilage destruction. The author recalls the origin, mechanism of action of the articular co...

journal_title：Annales de biologie clinique

authors： Mitrovic D

更新日期：1986-01-01 00:00:00

abstract：:We evaluated the analytical performances of the SPAPLUS(®) immunoturbidimeter assays manufactured by The Binding Site(®) for the quantification of thirteen immunological parameters in serum: IgG, IgA, IgM and IgD immunoglobulins, IgG subclasses (1 to 4), IgA subclasses (1 and 2), beta-2 microglobulin, free light chain...

journal_title：Annales de biologie clinique

authors： Thevenet I,Benat C,Chauzeix J,Blancher A,Puissant-Lubrano B

更新日期：2014-07-01 00:00:00

abstract：:Macroprolactin is a complex of prolactin with immunoglobulins (IgG) that has limited or no biological activity in vivo. Immunoassays for prolactin have variable reactivity with macroprolactin. Therefore the presence of macroprolactin should be considered in the differential diagnosis of hyperprolactinemia. We compared...

journal_title：Annales de biologie clinique

authors： Sapin R,Gasser F,Fischbach E,Grucker D

更新日期：2000-11-01 00:00:00

abstract：:We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A ...

journal_title：Annales de biologie clinique

authors： Pradalier N,Canal P,Pujol A,Fregevu Y,Soula G

更新日期：1982-01-01 00:00:00

abstract：:The analysis of longitudinal series of tumour markers during chemotherapy in patients with germ cells tumors is not easy. The purpose of this work is to present various characteristic profiles of the evolution of serum human chorionic gonadotropin during chemotherapy and review the modalities of the dynamic interpreta...

journal_title：Annales de biologie clinique

authors： Riedinger JM,Eche N,Fargeot P

更新日期：2008-09-01 00:00:00