Abstract：
:The aim of the present work was to assess the genetic and antigenic variability in the VP1 region of type 3 echviruses (E-3), an enterovirus serotype associated to meningitis, neuro-muscular diseases and type 1 diabetes in human. Forty-six VP1 sequences of E-3, among which 9 were isolated in tunisian infants, were included. Phylogenetic analyses and nucleotidic divergence rates were studied in the complete VP1 region and in a 290-nucleotides fragment in the 5' part of the P1. Aminoacid sequences were deduced in the aim to identify genotype-specific antigenetic determinants. E-3 sequences divided into two genogroups, I and II; the genetic variability within the E-3 serotype reached 29.1%. Genogroup I included sequences with a relatively high genetic diversity among each other, some of them grouped in one genotype with at most 15.1% divergence rate. The sequences included in Genogroup II have a maximum of 13.8% divergence corresponding probably to only one genotype. The two genogroups have a concomitant circulation and a wide geographical and temporal distribution. Aminoacid substitutions that may be specific to genogroups, genotypes and special variant were noted. This work provides a first tentative of classification of E-3 into genogroups and genotypes and reports new E-3 sequences from North Africa. It contributes to a better understanding of the molecular epidemiology of human enteroviruses, and of Echoviruses type 3 in particular, a serotype that remains insufficiently studied in the international literature.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Farès W,Rezig D,Yahia AB,Touzi H,Meddeb Z,Triki Hdoi
10.1684/abc.2012.0688subject
Has Abstractpub_date
2012-03-01 00:00:00pages
189-98issue
2eissn
0003-3898issn
1950-6112pii
abc.2012.0688journal_volume
70pub_type
杂志文章abstract：:AFP can be measured by radioimmunoassay in 52 hours using an incubation at 18 degrees C for 48 hours and the double antibody solid phase method to separate the free labelled AFP from the labelled AFP bound to the antibodies. In these conditions, the sensitivity and precision of the assay are very satisfactory and the ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Blood gas analyzers are able to provide information on a wide range of oxygenation parameters. Some of these data are complex. Their biological significance or clinical consistency are not always well known. This article aims to define these parameters, their reference values according to the type of sample; and to ou...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2017.1274
更新日期：2017-12-01 00:00:00
abstract：:We quantify the impact of several variables including site of blood draw, delay in measurement, and use of the cyanide antidote hydroxocobalamin on detection of both normal (<2 mmol/L) and elevated (>6 mmol/L) human plasma lactate. An in vivo study assessed effects of venous or arterial origin of blood samples. Two in...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2017.1310
更新日期：2018-01-01 00:00:00
abstract：:The purpose of this transverse prospective study was to determine blood nutritional, immunity and inflammatory proteins change in vitamin A deficiency in children of school-age (262 children, aged 7 to 15 years). Blood vitamin A has been determined by HPLC with UV detection. Proteins have been measured by radial immun...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:The experience of a specialized laboratory for the biological diagnosis of inborn errors of metabolism in selected pediatrics patients is reported. The strategy starts with a wide testing of blood and urine, as many inborn errors of metabolism can be detected through testing of blood and urine for increased concentrat...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1988-01-01 00:00:00
abstract：:French medical laboratories must be accredited before November 2016 according to NF/EN/ISO 15189 standard. However, technical accreditation guidelines cannot be applied literally for the determination of specific IgE for several reasons: more than 600 allergen tests, lack of international gold standard, limited extern...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2013.0830
更新日期：2013-05-01 00:00:00
abstract：:In biochemistry laboratory, monoclonal immunoglobulins are frequently found. They can lead to analytical interferences which are difficult to anticipate. In this case reports, a monoclonal immunoglobulin M with cryoglobulin effect has been responsible of false result of protein electrophoresis because of the whole pre...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:The development of enzymo-immunological technics and the synthesis of efficacious immuno-adsorbants has given rise to several immuno-enzymometric methods of which one, ELISA (enzyme-linked immunosorbent assay) has proved particularly practical and sensitive for the estimation of antibodies. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1978-01-01 00:00:00
abstract：:The non-secreting myeloma is a rare form of myeloma. Its association with a nephrotic syndrome is exceptional. The observation which we report below described the history of a 66 years old patient who presents a non-secreting myeloma revealed by a nephrotic syndrome. Various assumptions on the mechanism of no excretio...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:Circulating citrulline is emerging as an innovating biomarker candidate for assessment of intestinal function. Amino acid synthesized by enterocytes of intestinal mucosal, citrulline is not included in proteins or nutrition products and is a precursor for production of arginine by the kidney. Plasma citrulline is, in ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2011.0609
更新日期：2011-09-01 00:00:00
abstract：:The ISO 15189 standard requires the evaluation of measurement uncertainty where relevant and possible. This document presents recommendations for a simple evaluation of the uncertainty from data available in medical laboratory and illustrates the value of such a determination. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,实务指引
doi：10.1684/abc.2011.0561
更新日期：2010-12-01 00:00:00
abstract：:Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Acylcarnitine profile allows the identification and quantification of more than thirty compounds. As part of the accreditation process ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,实务指引
doi：10.1684/abc.2020.1582
更新日期：2020-10-01 00:00:00
abstract：:This paper describes the assessment of the homogeneity and stability of a purified and lyophilized human thyroglobulin (Tg), and characterizes its immunoreactivity. The purified and lyophilized Tg is intended to be used as a primary reference material to establish calibration of working serum based reference material....
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Myoadenylate deaminase deficit (MAD, MIM#615511) is the most common cause of metabolic myopathies with an estimated prevalence of 1-2% in the general population. We report the case of a 39-year-old man suffering from severe skeletal muscle pain that had developed gradually for 4 years. A moderate increase in creatine ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2017.1253
更新日期：2017-08-01 00:00:00
abstract：:We investigated two different freezing protocols on mouse embryos that were either grown in vivo or were grown in vitro for a certain period. Our results confirm that an extended in vitro culture period makes the embryo more susceptible for injury due to freezing and thawing. Furthermore when freezing two cell mouse e...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The multimeric glycoprotein Von Willebrand factor (vWF), is produced by vascular endothelium and platelet. If some constitutionals deficiencies leading to hemorrhagic syndrome have been explored in the literature, increased production of vWF observed during cellular distress and pregnancy have not been explored in our...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2014.0956
更新日期：2014-05-01 00:00:00
abstract：:Horseradish peroxidase is often used as an antibody-coupled enzyme and several procedures have been developed to obtain IgG-peroxidase conjugates. The most widely used are coupling with periodate or glutaraldehyde. To compare the efficiency of these methods, the authors conducted periodate coupling or glutaraldehyde c...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The analyzers have been defined as follows: the analyzers are multiparametric (at least 5 parameters available simultaneously), selective, operating patient by patient, list price on May 1, 1984 below 1,000,000 francs, and distributed in France after January 1, 1981. This definition applies in particular to systems al...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:International guidelines emphasize the importance of LDL cholesterol (LDL-C) assay in the care and follow-up of patients with cardiovascular risk. Most studies and common practice use Friedewald's formula for LDL-C calculation. The accuracy of the result depends closely on the precision of the input parameters (total ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,多中心研究
doi：
更新日期：2005-01-01 00:00:00
abstract：:Twenty four strains of coagulase-negative staphylococci from clinically significant blood cultures were separated in four groups on the basis of oxacillin susceptibility and phenotype of resistance to aminoglycoside antibiotics. Susceptibility testing included disk diffusion testing, M.I.C. and M.B.C. determinations a...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Eight Capnocytophaga infections are described: bacteremia in immunodepressed patients (three cases), endocarditis (one case), pneumopathy (one case), buccal infection (two cases) and endometritis during use of an intrauterine contraceptive device (one case). The role of this bacterium in infections presented by immuno...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:The purpose of this study was to evaluate the cut-off value of brain natriuretic peptide (BNP) assayed with AxSYM BNP assay (Abbott). 86 patients have been included (mean age of 66 years). The clinical sensibility was 100% at 100 ng/L versus 80% at 250 ng/L. The clinical specificity was 66% at 100 ng/L versus 92% at 2...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2005-03-01 00:00:00
abstract：:We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The SFBC working group aimed to deal with biological tests outside the French nomenclature that may be useful for the follow-up of dialysis patients. Our discussion was divided into 3 parts: 1) evaluation of peritoneal membrane characteristics; 2) monitoring of renal replacement therapy using regional citrate anticoag...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2019.1459
更新日期：2019-08-01 00:00:00
abstract：:A liquid chromatography procedure for routine determination of urinary estriol concentrations is described. After enzymic hydrolysis of a 1 ml sample, automatic extraction with chloroform/ethyl acetate, purification by chromatography on a disposable "minicolumn" of AG 1-X2, urinary estrogens are separated with normal ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Community viral bronchopneumonias are frequent, mainly in children, and can be associated to all respiratory viruses: influenza- and parainfluenzavirus, respiratory syncytial virus, adenovirus, rhinovirus. The diagnostic method which proves viral infection of the respiratory tissues is selected as the direct detection...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1998-01-01 00:00:00
abstract：:This paper points out the structural and physiological data of galectin-3, and emphasizes its role in cardiac fibrosis and heart failure pathophysiology. Then we summarize the optimal conditions for sampling, assays and we discuss the interpretation of results. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2014.0977
更新日期：2014-07-01 00:00:00
abstract：:An automatic continuous flow method is described for assay of plasmatic and urinary urea after hydrolysis by urease followed by NH4 assay with glutamic deshydrogenase in the same working solution. Precision and accuracy, comparison with chemical diacetylmonoxim method are studied. The interaction of endogenous NH4, th...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Elderly patients are at high risk of over-anticoagulation and of haemorrhagic risk when on warfarin, especially during treatment induction. In Charles Foix Hospital, a 800-bed geriatric hospital, we specifically developed for in-patients older than 70 years (target INR 2.5) a simple low-dose warfarin induction regimen...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:Using gas chromatography, we measured the concentrations of amino acids in a group of 80 selected subjects: we present the plasma values which we obtained, as well as those of other authors. Various comparisons were made, according to the age and/or sex of the subjects. We found that the plasma levels of valine, leuci...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
