Abstract：
:Twenty four strains of coagulase-negative staphylococci from clinically significant blood cultures were separated in four groups on the basis of oxacillin susceptibility and phenotype of resistance to aminoglycoside antibiotics. Susceptibility testing included disk diffusion testing, M.I.C. and M.B.C. determinations and finally, measurements, at sequential times (from 0 to 6 hours), of the bactericidal effect of aminoglycosides alone or combined with oxacillin or vancomycin. Aminoglycosides clinical use (gentamicin, netilmicin, tobramycin and amikacin) exhibited an efficient early bactericidy over the sensitive strains to any tested aminoglycosides and to oxacillin (group 1, 13 strains). When strains having a phenotype of resistance to kanamycin and neomycin (group 2, 4 strains) or kanamycin and tobramycin (group 3, 2 strains) were considered, amikacin inefficiency was assessed by time-killing assay even though disk diffusion testing as well as M.I.C. and M.B.C. determinations failed to demonstrate it. In the same way, only bactericidy measurements indicated the lack of activity of amikacin or netilmicin over strains exhibiting a phenotype of resistance to gentamicin plus kanamycin and tobramycin (group 4, 5 strains). Netilmicin appeared as the most active aminoglycoside over the strains studied. Crude results of disk diffusion testing might appear as an oversimplification, especially when coagulase-negative staphylococci are considered; the early-bactericidy assay findings were stressing for the need of interpreting the results of antibiotic testing on the ground of resistance mechanisms.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Truchet A,Minet J,Mamez C,Cormier Msubject
Has Abstractpub_date
1990-01-01 00:00:00pages
541-6issue
8eissn
0003-3898issn
1950-6112journal_volume
48pub_type
杂志文章abstract：:The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Diagnosis of systemic candidiasis is difficult and often performed lately. It can be improved by Candida antigen detection, using agglutination of latex particles sensitized with anti-Candida albicans antibody. Candida antigen search was made in 407 sera obtained from 123 patients, in Hematology and in intensive care ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:In biochemistry laboratory, monoclonal immunoglobulins are frequently found. They can lead to analytical interferences which are difficult to anticipate. In this case reports, a monoclonal immunoglobulin M with cryoglobulin effect has been responsible of false result of protein electrophoresis because of the whole pre...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:We report the case of an 83-year-old woman admitted to the accident and emergency unit for pneumopathy. The blood cell count on the DXH automaton (Coulter® ™ UniCel® DxH) found a normochromic and normocytic anemia, anormal platelet count and subnormal leukocyte formula. The only alarm raised by the automaton was the p...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2018.1406
更新日期：2019-04-01 00:00:00
abstract：:Two cases of newborn haemolytic disease because of ABO incompatibility are reported. In both cases, blood erythroblastosis exceeded 300 per 100 leukocytes at birth. Both newborns developed early jaundice and one presented with anaemia. Intensive phototherapy begun soon after birth was an effective treatment. The diagn...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Total plasma homocysteine emerged in the past few years as an independent risk factor for cardiovascular diseases. This test is now currently prescribed for the diagnosis of unexplained thrombosis in young adults or recurrent thrombosis in patients with arteriopathy. This sulphured amino-acid is an important intermedi...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：:The presence of the R506Q mutation of the factor V gene is associated with an increased risk of thromboembolism, particularly during pregnancy. Recently, its involvement in the development of obstetrical complications, such as preeclampsia and fetal losses, has been evoked. The resulting factor VQ506 (factor V Leiden)...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Li Fraumeni Syndrome (LFS) is a rare autosomal disorder characterized by a familial clustering of tumors. Analysis of several series of LFS families have shown that 70% of such families are attributable to germ-line mutations in TP53. We report the case of a patient who had a first degree family antecedent of cancer i...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2010.0441
更新日期：2010-05-01 00:00:00
abstract：:The experience of a specialized laboratory for the biological diagnosis of inborn errors of metabolism in selected pediatrics patients is reported. The strategy starts with a wide testing of blood and urine, as many inborn errors of metabolism can be detected through testing of blood and urine for increased concentrat...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1988-01-01 00:00:00
abstract：:Adult neutropenia, defined as a blood neutrophil count below 1.5 G/L, is a common condition. The most common cause of acute neutropenia is a drug-related reaction or an acute infectious disease. In chronic forms many etiologies are possible, sometimes poorly-defined, requiring precise explorations. The purpose of this...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2018.1393
更新日期：2018-12-01 00:00:00
abstract：:A reversed-phase high-performance liquid chromatographic assay for the determination of the HIV protease inhibitors amprenavir (Agenerase) and indinavir (Crixivan) in human plasma is described, using a mobile phase consisting of 0.50 M phosphate buffer (adjusted to pH 5,5) - Milli-Q water - acetonitrile (120: 1,080: 8...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:The non-secreting myeloma is a rare form of myeloma. Its association with a nephrotic syndrome is exceptional. The observation which we report below described the history of a 66 years old patient who presents a non-secreting myeloma revealed by a nephrotic syndrome. Various assumptions on the mechanism of no excretio...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:In order to introduce the approach of HCV genotyping in our laboratory, a comparative study of 3 molecular and 1 serological methods, was conducted on 62 HCV RNA positive sera. The molecular genotyping methods target the 5'untranslated (UTR) region of the virus genome and are based on an amplification of the viral gen...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Salmonelloses are becoming more frequented. To date, very few cases of aneurisms or vascular grafts infections have been described. We report the rare case of an extradigestive manifestation, due to Salmonella enteritidis affecting a patient with Behçet disease. The biological diagnosis is based on microorganism isola...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2010.0405
更新日期：2010-01-01 00:00:00
abstract：:The discriminatory potency of variable number of tandem repeats (VNTR), based on 7 loci (MIRU 26, 27 and 5 ETRs A, B, C, D, E) was assayed on Mycobacterium bovis strains obtained from samples due to tuberculosis in two slaughterhouses in Algeria. The technique of MIRU-VNTR has been evaluated on 88 strains of M. bovis ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2010.0459
更新日期：2010-07-01 00:00:00
abstract：:Strongyloïdes stercoralis infection is a polymorphic and non specific clinical presentation. Often asymptomatic, it can be not seen. However, in patients with immunodeficiency, high parasite load can be observed, consequence of self-infestation cycle, and can spread throughout the body. This presentation of malignant ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2013.0809
更新日期：2013-03-01 00:00:00
abstract：:Macroprolactin is a complex of prolactin with immunoglobulins (IgG) that has limited or no biological activity in vivo. Immunoassays for prolactin have variable reactivity with macroprolactin. Therefore the presence of macroprolactin should be considered in the differential diagnosis of hyperprolactinemia. We compared...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecu...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2013.0891
更新日期：2013-09-01 00:00:00
abstract：:The aim of this study was to evaluate the pre-analytical factors contributing to uncertainty in thyroglobulin measurement in fluids from fine-needle aspiration (FNA) washout of cervical lymph nodes. We studied pre-analytical stability, in different conditions, of 41 samples prepared with concentrated solutions of thyr...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2017.1225
更新日期：2017-04-01 00:00:00
abstract：:Horseradish peroxidase is often used as an antibody-coupled enzyme and several procedures have been developed to obtain IgG-peroxidase conjugates. The most widely used are coupling with periodate or glutaraldehyde. To compare the efficiency of these methods, the authors conducted periodate coupling or glutaraldehyde c...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:The search for sperm is part of the examinations wich may be prescribed, following a request of jucicial services, in case of a suspicion of sexual assault. This article applies to detail how the different phases, pre-analytical, analytical and post-analytical, must be absolutely adhered to so that the biologist, expe...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2008.0274
更新日期：2008-09-01 00:00:00
abstract：:Depression is a pathology frequently observed in general medicine (10%). Treatment of depression makes great use of drugs from different pharmacological classes. The optimal posology is difficult to establish, and clinicians prefer to avoid side effects by prescribing low regimen. This study is related to an evaluatio...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Blood testing using the dried blood spot (DBS) is used since the 1960s in clinical analysis, mainly within the framework of the neonatal screening (Guthrie test). Since then numerous analytes such as nucleic acids, small molecules or lipids, were successfully measured on the DBS. While this pre-analytical method repre...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2014.1007
更新日期：2015-01-01 00:00:00
abstract：:Exploring coagulation in newborns and children is a challenge due to the low levels of both procoagulant factors and inhibitors. Conventional coagulation tests might be inadequate to explore all of these changes. The aim of the study is to evaluate the thrombin generation assay, a global test to explore coagulation, i...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2020.1607
更新日期：2020-12-01 00:00:00
abstract：:Recently, homocysteine and new inflamatory biomarkers are demonstrated to be involved in cardiovascular diseases. These risk factors are not well studied in acute coronary syndrome. We investigated the distribution of homocysteine and inflammatory markers in patients with acute coronary syndrome and evaluated the asso...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2011.0533
更新日期：2011-03-01 00:00:00
abstract：:Blood histamine proved to be important for the immune homeostasis. By its direct action or by means of H-2 receptors it end or prevents the excessive immune response. Recent studies revealed changes in cell mediated immunity in subjects with atopia, with increased histaminemia and their obvious protection against canc...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：UNLABELLED:The aim of our study was to evaluate the sensitivity, specificity and predictive values of isolated and combined ASCA and NANA assays in Tunisian inflammatory bowel diseases patients and to assess whether their presence is associated with particular clinical features of the disease. METHODS:128 patients (39...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2010.0471
更新日期：2010-09-01 00:00:00
abstract：:The identification of polymorphism A4059V associated with the 12276 A>G at exon 45 of the PKD1 gene in a Tunisian polycystic patient. ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2015.1036
更新日期：2015-03-01 00:00:00
abstract：:SFBC working group on critical care testing describes in this paper guideline for the management of laboratory medicine examination process in emergency conditions. After a summary on French standards and regulations, the critical care testing perimeter and definitions of stat levels are presented in different context...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,实务指引
doi：10.1684/abc.2015.1113
更新日期：2016-03-01 00:00:00
abstract：:The stimulation regimens and the results of ovulation induction in anovulatory patients and in patients suffering from a Luteinized Unruptured Follicle (LUF) syndrome are discussed as well as the findings concerning superovulation in IVF cycles. The percentage of multiple pregnancies (less than or equal to 20 p. cent)...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
