Abstract:
:Total plasma homocysteine emerged in the past few years as an independent risk factor for cardiovascular diseases. This test is now currently prescribed for the diagnosis of unexplained thrombosis in young adults or recurrent thrombosis in patients with arteriopathy. This sulphured amino-acid is an important intermediate in transsulfuration and remethylation pathways of methionine metabolism. Within the context of a collaboration between Monastir and Grenoble Universities and because a gas chromatograph mass spectrometer (GC-MS) instrument was available in Monastir, we proposed to transpose a GC-MS method previously developed in Grenoble's hospital for this parameter and to validate it by comparison with the liquid chromatography tandem mass spectrometry (LC-MS-MS) method, used at present. Analytical performances were good: detection limit 0.4 micromol/L and linear range up to 4 mg/L (29.6 micromol/L), and between-run and within-run precision with coefficients of variation < 5% and < 8 %, respectively. The comparison with LC-MS-MS method showed a good correlation (y = 0.9874 x -0.208; r(2) = 0.84). Mean difference from LC-MS-MS was -0.4 micromol/L. Plasma concentrations of homocysteine (mean + SD) determined among Tunisian adults, 29 men, 27 women, of the same age were respectively: 11.6 +/- 2.4 micromol/L and 10.1 +/- 2.7 micromol/L, p = 0.025. This method is now currently used to evaluate tHcy concentration in patients with risk factors for cardiovascular disease.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Belkhiria MN,Ducros V,Harzallah K,Jarraya F,Cordonnier D,Favier A,Achour Asubject
Has Abstractpub_date
2007-07-01 00:00:00pages
393-8issue
4eissn
0003-3898issn
1950-6112journal_volume
65pub_type
杂志文章abstract::Bacillus alvei hemolytic toxin has been characterized in five main steps from a 18 hours culture supernatant fluid. 50% ammonium sulfate precipitations are followed by Sephadex gel filtrations. After that we proceed to an isoelectric focusing in a narrow pH gradient (from 4 to 6). A final step consists in a gel filtra...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,多中心研究
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更新日期:2005-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.0979
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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journal_title:Annales de biologie clinique
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doi:
更新日期:1981-01-01 00:00:00
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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