Abstract：
UNLABELLED:The aim of our study was to evaluate the sensitivity, specificity and predictive values of isolated and combined ASCA and NANA assays in Tunisian inflammatory bowel diseases patients and to assess whether their presence is associated with particular clinical features of the disease. METHODS:128 patients (39 with ulcerative colitis, 25 with Crohn disease, and 64 healthy subjects) were enrolled in the study. ASCA and NANA were performed by ELISA and indirect immuno-fluorescence, respectively. Sensitivity, specificity, PPV and NPV of NANA for UC was: 56.4%, 88%, 88% and 56.4%; and ASCA for CD were: 56%, 82.1%, 54.5% and 75.6%, respectively. The combined use of these two markers modified diagnostic accuracy: NANA+/ASCA- in UC 53.8%, 88%, 87.5% and 55% and for NANA-/ASCA+ in CD 52%, 94.9%, 86.7% and 75.5%. No significant association was found between the presence of a specific serological marker and patients' clinical features. In conclusion, the specificity of these combined markers tends to be higher than sensitivity, what made them more useful in the differentiation of the IBD subtypes rather than population screening.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Bouzid D,Haddouk S,Amouri A,Ben Haj Hmida Y,Boukthir S,Ben Ayed M,Tahri N,Masmoudi Hdoi
10.1684/abc.2010.0471subject
Has Abstractpub_date
2010-09-01 00:00:00pages
577-83issue
5eissn
0003-3898issn
1950-6112pii
abc.2010.0471journal_volume
68pub_type
杂志文章abstract：:Glycated hemoglobin can be assayed by methods using different principles, whose performances vary and are difficult to control. The most widely used techniques measure either specifically glycated A1c hemoglobin (HbA1c) or total glycated hemoglobin. They are reviewed in detail in this paper and standardization problem...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1994-01-01 00:00:00
abstract：:Horseradish peroxidase is often used as an antibody-coupled enzyme and several procedures have been developed to obtain IgG-peroxidase conjugates. The most widely used are coupling with periodate or glutaraldehyde. To compare the efficiency of these methods, the authors conducted periodate coupling or glutaraldehyde c...
journal_title：Annales de biologie clinique
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doi：
更新日期：1995-01-01 00:00:00
abstract：:The simultaneous presence of homogeneous and heterogeneous reactions at different binding sites of a multiepitope antigen makes the description of the kinetic parameters of the so called "one step" solid phase immunometric assays complex. The authors extended the "one step" approach to the concept of the "soluble sand...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:In assisted reproductive technology (ART), serum levels of several key hormones are used to evaluate ovarian follicular reserve and to monitor gonadotropin-stimulated follicle growth. Currently, serum estradiol, FSH, LH and inhibin B levels are examined and combined at the beginning of the menstrual cycle to evaluate ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：2003-09-01 00:00:00
abstract：:Hydrolysis of a solution of amylopectin by serum or urinary amylase leads to a reduction of the turbidity of this solution, observed at 340 nm. Adapted to centrifugal analysis, this method permits 15 estimations in 3 minutes with a serial reproducibility of 2.7 per cent, and a daily reproducibility of 6 per cent. The ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Premature rupture of the membranes (PROM) is a frequent event affecting 3% of pregnancies. PROM causes 30% of premature deliveries and 20% of perinatal mortality. The diagnosis relies mainly on the clinical visualization of the amniotic fluid flow in the vagina. If not, clinicians can use bedside tests detecting eithe...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2018.1346
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1993-01-01 00:00:00
abstract：:Iron plays a fundamental role in biology and its concentration in living organisms is regulated very precisely. Many molecules of storage and transportation are used to maintain the intracellular homeostasis. Cancer cells have alterations in this balance. Recent studies have shown that breast cancer cells present abno...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2012.0700
更新日期：2012-07-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Mycoplasma hominis, a mycoplasma potentially isolated from the genital tract of healthy women, seems to have some role in pelvic inflammatory diseases (PID). Three serologic techniques were developed to detect antibodies to M. hominis: ELISA (IgG, IgM), microimmunofluorescence (MIF) and Western blot. Rabbit and human ...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractio...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:A recently commercialized dot-blot (Cyto-Dot, BMD) offered a new method for the detection of three autoantibodies (Ab) anti-Jo-1, anti-M2, and anti-ribosomal protein although their only common point is the cytoplasmic localisation of their respective antigen. These Ab are detected by indirect immunofluorescence (IF) (...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2019.1445
更新日期：2019-06-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2011.0533
更新日期：2011-03-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Patients with renal insufficiency who are hemodialyzed 3 times a week either through a cuprophane membrane or through a polyacrylonitrile (PAN) membrane have the same transcortin and cortisol levels as patients with renal insufficiency who are not dialyzed. Moreover, hemodialysis did not influence acutely the latter t...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The aim of this study was to evaluate a new automated fluorimetric enzyme immunoassay for CK-MB using the Vidas bioMérieux. The results for sera from 222 patients were compared with those obtained using two other methods of CK-MB determination in mass units (Stratus II Dade, Magic Lite Analyzer II Ciba Corning). Satis...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
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journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1982-01-01 00:00:00
abstract：:We evaluated a rapid and semi-quantitative C-Reactive Protein test on whole blood, the Actim CRP (Fumouze). Based on immuno-chromatography technology, this test ranked the blood sample in four groups: < 10 mg/L, 10-40 mg/L, 40-80 mg/L and > 80 mg/L. This evaluation finds an excellent repeatability, the absence of hook...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Blood gas analyzers are able to provide information on a wide range of oxygenation parameters. Some of these data are complex. Their biological significance or clinical consistency are not always well known. This article aims to define these parameters, their reference values according to the type of sample; and to ou...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：10.1684/abc.2017.1274
更新日期：2017-12-01 00:00:00
abstract：:The great diversity of methods for manganese analysis in biological materials (serum, plasma, whole blood, urine, spinal fluid and hair) reveals the difficulty in measuring extremely small quantities of this element. An examination of these methods demonstrate that the most used one is flameless atomic absorption spec...
journal_title：Annales de biologie clinique
pub_type： 杂志文章,评审
doi：
更新日期：1988-01-01 00:00:00
abstract：:The diagnosis of double hit lymphoma remains a challenge for the biologist for a good management of the patient. This new category of lymphoma "double hit" (DH) is part of a new entity of the WHO classification 2008: « Unclassifiable B lymphoma with features intermediate between diffuse large cell B lymphoma and Burki...
journal_title：Annales de biologie clinique
pub_type： 杂志文章
doi：10.1684/abc.2016.1189
更新日期：2016-12-01 00:00:00
