[A liquid chromatography technique for routine determination of urinary estriol in human pregnancy (author's transl)].

Abstract:

:A liquid chromatography procedure for routine determination of urinary estriol concentrations is described. After enzymic hydrolysis of a 1 ml sample, automatic extraction with chloroform/ethyl acetate, purification by chromatography on a disposable "minicolumn" of AG 1-X2, urinary estrogens are separated with normal phase column and isocratic elution with isooctane/ethyl acetate/methanol/water and the optical density at 280 nm is measured. The specificity is assessed by comparison of liquid chromatography with fluorometry and gas liquid chromatography. The technique is simple, rapid and quite suitable for routine determination.

journal_name

Ann Biol Clin (Paris)

authors

Habrioux G

subject

Has Abstract

pub_date

1981-01-01 00:00:00

pages

75-9

issue

2

eissn

0003-3898

issn

1950-6112

journal_volume

39

pub_type

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