Abstract:
:We evaluated the analytical performances of the SPAPLUS(®) immunoturbidimeter assays manufactured by The Binding Site(®) for the quantification of thirteen immunological parameters in serum: IgG, IgA, IgM and IgD immunoglobulins, IgG subclasses (1 to 4), IgA subclasses (1 and 2), beta-2 microglobulin, free light chains kappa and lambda. The within-day precision (repeatability) and the between-day precision (reproducibility) were obtained for two or three concentration levels depending of the parameter and were below the recommendations of the manufacturer, except for the repeatability of IgG1 (at a level of concentration of 6.7 g/L). An agreement above 90% (with Bland and Altman analysis) was observed between the results obtained with SPAPLUS(®) and those obtained with the nephelometer IMMAGE(®) 800 or radial immunodiffusion. The evaluation confirmed the linearity of the assays and the absence of contamination for all the parameters tested. We also assessed the practicability of the SPAPLUS(®) in terms of maintenance, frequency of calibration and cadence tests. The SPAPLUS(®) immunoturbidimeter displays good analytical performances for the immunological parameters evaluated in the present work.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Thevenet I,Benat C,Chauzeix J,Blancher A,Puissant-Lubrano Bdoi
10.1684/abc.2014.0974subject
Has Abstractpub_date
2014-07-01 00:00:00pages
423-34issue
4eissn
0003-3898issn
1950-6112pii
abc.2014.0974journal_volume
72pub_type
杂志文章abstract::Blood histamine proved to be important for the immune homeostasis. By its direct action or by means of H-2 receptors it end or prevents the excessive immune response. Recent studies revealed changes in cell mediated immunity in subjects with atopia, with increased histaminemia and their obvious protection against canc...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
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abstract::Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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journal_title:Annales de biologie clinique
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doi:
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
abstract::This document is a proposal of questionnaire for a self-assessment of the post-examination phase: results validation, reporting and transmitting, post-examination samples keeping and documents archival storage. The questions allow to check that the laboratory fulfils the ISO 15189 Standard and COFRAC SH REF 02 documen...
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2015.1066
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2011.0598
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1684/abc.2009.0348
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2008.0210
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
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doi:10.1684/abc.2017.1225
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
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journal_title:Annales de biologie clinique
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doi:
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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doi:
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
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