Abstract：
OBJECTIVE:to develop a rapid and reliable real-time PCR to detect polymorphisms of angiotensinogen (AGT), to compare the two methods of MS-PCR (Mutagenically Separated PCR) and real-time PCR to determine three polymorphisms of the angiotensinogen gene M235T, the A (-6) G and A (-20) C. METHODS:the method of real-time PCR was developed on the PLC Roche LightCycler1 with SYBR Green I. We used two sense primers and a primer nonsense. Detection of polymorphisms of angiotensinogen gene was performed by comparing the melting curves. RESULTS:the DNA samples were analyzed by two methods: real-time PCR and MS-PCR. In our study, no differences were found between the two techniques. DISCUSSION:The real-time PCR is a rapid and reliable method for detecting gene polymorphisms on the AGT M235T, the A (-6) G and A (-20) C. CONCLUSION:this method of real-time PCR is a reliable genetic test, which is fast and cheap and can be used in practice to study particular polymorphisms of AGT gene associated with cardiovascular disease.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Dandana A,Gammoudi I,Ferchichi S,Chahed H,Bricca G,Limem HB,Maatouk F,Miled Adoi
10.1684/abc.2011.0598subject
Has Abstractpub_date
2011-07-01 00:00:00pages
447-51issue
4eissn
0003-3898issn
1950-6112pii
abc.2011.0598journal_volume
69pub_type
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