Abstract:
:An automated kinetic method to determine creatinine in plasma with Jaffé reaction (alkaline picrate reagent) using a Gemsaec analyser is studied in order to argue the precision and the accuracy. The analysis of the reaction rate of a primar standard solution and of different plasmas suggests that a rapid measure is requisite in order to avoid interferences from chromogens contained in plasma which develop a same but often slower and durabler coloration at the used wavelength. The method is compared to the manual procedure using adsorption on Lloyd's reagent.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Klumpp T,Férard Gsubject
Has Abstractpub_date
1977-01-01 00:00:00pages
203-8issue
3eissn
0003-3898issn
1950-6112journal_volume
35pub_type
杂志文章abstract::The aim of the present work was to assess the genetic and antigenic variability in the VP1 region of type 3 echviruses (E-3), an enterovirus serotype associated to meningitis, neuro-muscular diseases and type 1 diabetes in human. Forty-six VP1 sequences of E-3, among which 9 were isolated in tunisian infants, were inc...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2012.0688
更新日期:2012-03-01 00:00:00
abstract::Until now, the methods of analysis by luminescence have not developed greatly in clinical biology because of the instability of the reagents and the absence of a single protocol applicable to a sufficiently wide range of assays. For this reason, the application has been limited to several assays using a specific biolu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1985-01-01 00:00:00
abstract::A previous multicentric study set up by the Société française de biologie clinique has emphasized the usefulness of a standardized procedure for the determination by high performance liquid chromatography of alpha-tocopherol in serum or plasma. In our study, we have tested every step of the different published procedu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
abstract::We report a turbidimetric procedure, using commercially available reagents, for the determination of plasma fibronectin with an unmodified centrifugal analyser. Fixed-time kinetic readings at 340 nm were taken at 60 and 720 s. A wide range of concentration (up to 1000 mg/l) can be measured. Precision and accuracy were...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Apolipoprotein B (apoB) is the major proteic component of LDL, VLDL and chylomicrons. Numerous polymorphisms of the apolipoprotein B gene have been described. Particularly, a polymorphism of insertion/deletion located in the coding part of the signal peptide of apoB, associated with modifications of lipid concentratio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
abstract::M.I.S.-Enterobacteriaceae is a new kit for identifying Enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. The validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The authors have studied several seric, plasmatic and urinary constituents in patients hospitalized for an acute abdominal syndrome to be able to characterize an eventual pancreatic lesion; mainly seric and urinary amylase as well as its isoenzymes, lipase, liver profile and trypsin. In acute pancreatitis, the means o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Evaluation of the neonates for jaundice and kernicterus is indispensable when early hospital discharge has become standard practice. Transcutaneous bilirubin (TcB) measurement is an advantageous option because of its non-invasive nature and the possibility of its use near the patient. The point of care device BiliChec...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::The neuropathies associated with monoclonal IgM gammopathy reacted with glycoconjugated targets on a very antigenic epitope on the sulfated glucuronic glycolipids corresponding to SGPG and SGLPG (sulfoglucuronyl paragloboside and sulfoglucuronyl lactosaminyl paragloboside), myelin-associated glycoprotein (MAG) and sul...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Monoclonal antibodies have been raised against porcine LH receptor and allowed to clone the corresponding messenger RNA from testicular cells. Cross hybridisation with the LH receptor clone allowed to isolate a clone corresponding to the human TSH receptor from thyroids. The structure of both receptors have been deter...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1992-01-01 00:00:00
abstract::Gas-liquid chromatography has been used directly on clinical specimens as a method for presumptive diagnosis of anaerobic infections. This technique proved to be valuable and reliable. Combined with a Gram stain, it offers the possibility to differentiate in less than 1 hour aerobic from anaerobic infections by means ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Anti native DNA antibodies (anti nDNA Ab), which are a highly specific feature of systemic lupus erythematosus (SLE) were measured by 3 methods: an enzyme linked immunosorbent assay (ELISA), an indirect immunofluorescence test on Crithidia luciliae (IFCL) and the Farr assay (reference test). 114 sera from patients wit...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Recent advances in the characterization of microcrystals in tissue samples based on physico-chemical techniques are reviewed. It is a new opportunity for the physician to access early a diagnosis of rare but severe pathologies and to start a specific therapy able to delay or to stop an irreversible alteration of the o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2015.1070
更新日期:2015-09-01 00:00:00
abstract::A reversed-phase high-performance liquid chromatographic assay for the determination of the HIV protease inhibitors amprenavir (Agenerase) and indinavir (Crixivan) in human plasma is described, using a mobile phase consisting of 0.50 M phosphate buffer (adjusted to pH 5,5) - Milli-Q water - acetonitrile (120: 1,080: 8...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Toxicological screening is a specific approach to analytical toxicology that uses analytical tools such as GC-MS, LC-UV (diode array) or LC-MS. Toxicological screening allows the detection and simultaneous identification of a large number of compounds. The results may be based on the use of one or more techniques. As ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,实务指引
doi:10.1684/abc.2019.1422
更新日期:2019-04-01 00:00:00
abstract::Diagnosis of type III hyperlipoproteinemia requires preparative ultracentrifugation in order to measure the cholesterol/triglyceride molar ratio into isolated d less than 1.006 lipoproteins. The authors describe an ultracentrifugation micromethod which needs 600 microliter of serum and can be completed within three ho...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::Today, medical prescriptions of proteinuria or albuminuria, which are depending on medical status of patients, still depend on doctors. Indeed, according to their medical specialty or to the recommendations they are following, they could prescribe either proteinuria or albuminuria. The consequences are practical for l...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2019.1412
更新日期:2019-02-01 00:00:00
abstract::The diagnosis of non ST elevation myocardial infarction (NSTEMI) is very important for the emergency doctor. According to the literature copeptin, a marker of the endogenous stress, combined with troponin could be of interest in this diagnosis. The objective of the study was to investigate the association of high-sens...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0884
更新日期:2013-09-01 00:00:00
abstract::The present importance of streptococcal pathology implicates the identification of the streptococcal groups. The authors have carried out a comparative study of the various methods of extraction of the group specific antigen. They demonstrated the advantages and disadvantages of each method and consider the pronase ex...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::The relations between chromosomal diseases and mental retardation can be envisaged in three different ways: 1) Pharmacological approach shows a peculiar sensitivity of the cholinergic system in trisomic 21 children. A trouble in the manufacture of cholinergic mediator is plausible. 2) General biochemistry shows a slig...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::The authors present the interest of electrosyneresis for diagnosis of purulent meningitis. Test material is a study about 120 cerebrospinal fluids. Electrosyneresis sometimes permits to identify the germ when microscopic examination and culture are negative or when microscopy is positive and the culture negative. Appl...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::A fluorimetric, authomatical method for determination of free and total cholesterol in serum is described. Cholesterol esters are splitted into free cholesterol and fatty acids by cholesterol esterase. In the presence of oxygen free cholesterol will be transformed by cholesterol oxydase in delta 4 cholestenone with fo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2017.1283
更新日期:2017-12-01 00:00:00
abstract::The aim of this study was to evaluate the pre-analytical factors contributing to uncertainty in thyroglobulin measurement in fluids from fine-needle aspiration (FNA) washout of cervical lymph nodes. We studied pre-analytical stability, in different conditions, of 41 samples prepared with concentrated solutions of thyr...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2017.1225
更新日期:2017-04-01 00:00:00
abstract::The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Mycoplasma hominis, a mycoplasma potentially isolated from the genital tract of healthy women, seems to have some role in pelvic inflammatory diseases (PID). Three serologic techniques were developed to detect antibodies to M. hominis: ELISA (IgG, IgM), microimmunofluorescence (MIF) and Western blot. Rabbit and human ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::In this study the authors compare two automated coagulation instruments: the koagulab-40A (Orthodiagnostics Systems) and l'ACL-100 (Instrumentation Laboratory) using two different methods of detection, photo-optical and nephelemetric respectively. The three parameters studied are: prothrombin time (PT) (82 samples), a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::We resume here the guidelines about sample processing and transport for laboratory tests. All the steps of the pre-examination phase process, downstream to the recording of the requests, are described in this document. The purpose is to assure a secure and optimal sample management. The traceability of all the steps o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,实务指引
doi:10.1684/abc.2011.0549
更新日期:2010-12-01 00:00:00
abstract::Serum digoxin measurement is often performed in medical laboratories. A professional association specialized in quality control, based in Lyon, has been organizing punctual controls of medication measurement for the past ten years. The results are analysed in term of intra and inter-technique precision, difference bet...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::Horseradish peroxidase is often used as an antibody-coupled enzyme and several procedures have been developed to obtain IgG-peroxidase conjugates. The most widely used are coupling with periodate or glutaraldehyde. To compare the efficiency of these methods, the authors conducted periodate coupling or glutaraldehyde c...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00