[Isoelectrofocusing in the study of the heterogeneity of immunoglobulins G in man with or without proliferative symptomatology].

abstract：:Nowadays, physiological amino acids profiling is based primarily on ion exchange chromatography (IEC) coupled to a post-column derivatization with ninhydrin and UV detection at two wavelengths. Unfortunately, this technique suffers various drawbacks such as long analysis time, high sample volume and specific costs rel...

journal_title：Annales de biologie clinique

authors： Boemer F,Schoos R,Deberg M

更新日期：2015-07-01 00:00:00

abstract：:Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...

journal_title：Annales de biologie clinique

authors： Drénou B,Fardel O,Fauchet R,Amiot L

更新日期：2002-11-01 00:00:00

abstract：:We have evaluated the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie (edition2007), using to AGREE criteria, which are consensual at an international level, in particular at the the World Health Organisation (WHO) and at the European ...

journal_title：Annales de biologie clinique

authors： Fonfrède M,Couaillac JP,Augereau C,De Moüy D,Lepargneur JP,Szymanowicz A,Watine J

更新日期：2011-03-01 00:00:00

abstract：:Hemolysis should lead to changes in test results. Our study evaluated the impact of hemolysis on 26 blood measurements of stat biochemistry markers (sodium, potassium, chloride, urea, creatinine, glucose, total protein, calcium, magnesium, inorganic phosphorus, uric acid, C-reactive protein, total bilirubin, ASAT, ALA...

journal_title：Annales de biologie clinique

authors： Poupon C,Lefèvre G,Ngo-François S,Alibeu C,Barbé F,Bourbonneux V,Cartier R,Morin C,Szymanowicz A,Vuillaume I,Groupe de travail du Collège national de biochimie des hôpitaux.

更新日期：2015-11-01 00:00:00

abstract：:Anti-erythrocyte alloimmunization may occur following the transfusion of platelet concentrates, in response to the presence of residual erythrocytes. Immunization against RH1 (D) antigen is the most frequent, but transfusion of RH1 compatible platelet concentrates is not always possible because of supply constraints. ...

journal_title：Annales de biologie clinique

authors： Duboeuf S,Flourié F,Courbil R,Oriol P,Benamara A,Chartier M,Rigal E,Garraud O

更新日期：2009-05-01 00:00:00

abstract：:Hemoglobin Alc is the result of non-enzymatic binding of a glucose molecule at the NH2 terminal of the beta chain. Assay of glycosylated hemoglobin is of great utility for the control efficacity of antidiabetic treatments, since such levels provide the clinician with an excellent index of glucose metabolism over the w...

journal_title：Annales de biologie clinique

authors： Wajcman H,Kitzis A

更新日期：1981-01-01 00:00:00

abstract：:This article aims to place the phosphatidylethanol (PEth) blood test in the detection area of ethanol consumption causing alcohol-related disorders, to present the current methods of analysis, data on interpretation, some practical applications and the prospects of use of this biomarker. PEth is a minor metabolite of ...

journal_title：Annales de biologie clinique

authors： Hakim F,Wiart JF,Ménard O,Allorge D,Gaulier JM

更新日期：2019-12-01 00:00:00

abstract：:Biermer's disease is an autoimmune atrophic gastritis of the fundus predominantly responsible for a malabsorption of vitamin B12. Despite its association with several autoimmune disorders, few observations have reported an association with autoimmune hemolytic anemia (AIHA). We report a case of Biermer's disease assoc...

journal_title：Annales de biologie clinique

authors： Nafil H,Tazi I,Mahmal L

更新日期：2012-07-01 00:00:00

abstract：:In our societies, the increase of the frequency of the diseases of overweight, in particular obesity, diabetes type 2 and metabolic syndrome, coincides with that of the urinary lithiasis. Like the lithiasic disease, the metabolic syndrome or syndrome X is multi-factor. Several epidemiological studies were interested i...

journal_title：Annales de biologie clinique

authors： Zerifi R,Bahlous A,Marakchi O,Daudon M,Bartagi Z,Abdelmoula J

更新日期：2008-01-01 00:00:00

abstract：:Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Amino acid chromatography allows the identification and quantification of more than forty compounds. As part of the accreditation proce...

journal_title：Annales de biologie clinique

authors： Veauville A,Acquaviva-Bourdain C,Nowoczyn M,Read MH,Corne C,Dessein AF,Van Noolen L,Garnotel R,Minet-Quinard R

更新日期：2020-10-01 00:00:00

abstract：:Adult neutropenia, defined as a blood neutrophil count below 1.5 G/L, is a common condition. The most common cause of acute neutropenia is a drug-related reaction or an acute infectious disease. In chronic forms many etiologies are possible, sometimes poorly-defined, requiring precise explorations. The purpose of this...

journal_title：Annales de biologie clinique

authors： Bonnin A,Vignon G,Mottaz P,Labrousse J,Carrere F,Augereau PF,Aucher P,Lellouche F

更新日期：2018-12-01 00:00:00

abstract：:The identification of polymorphism A4059V associated with the 12276 A>G at exon 45 of the PKD1 gene in a Tunisian polycystic patient. ...

journal_title：Annales de biologie clinique

authors： Sahnoun S,Barbouch S,Hadj Fredj S,Khedher A,Messaoud T

更新日期：2015-03-01 00:00:00

abstract：:Determination of sex-hormone-binding-globulin was performed by three methods: binding, electroimmunodiffusion and DELFIA methods. Results of normal and pathological values correlated well with the three techniques. ...

journal_title：Annales de biologie clinique

authors： Patricot MC,Mathian B,Later F,Revol A

更新日期：1990-01-01 00:00:00

abstract：:Blood gas analyzers are able to provide information on a wide range of oxygenation parameters. Some of these data are complex. Their biological significance or clinical consistency are not always well known. This article aims to define these parameters, their reference values according to the type of sample; and to ou...

journal_title：Annales de biologie clinique

authors： Godignon M,Costes F,Sapin V,Bouvier D

更新日期：2017-12-01 00:00:00

abstract：:The present importance of streptococcal pathology implicates the identification of the streptococcal groups. The authors have carried out a comparative study of the various methods of extraction of the group specific antigen. They demonstrated the advantages and disadvantages of each method and consider the pronase ex...

journal_title：Annales de biologie clinique

authors： Bergogne-Berezin E,Slim A

更新日期：1976-01-01 00:00:00

abstract：:The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...

journal_title：Annales de biologie clinique

authors： Van Cutsem J,Van Gerven F,Janssen PA

更新日期：1987-01-01 00:00:00

abstract：:Rheumatoid arthritis is the most common and most severe form of inflammatory arthritis. Its exact aetiology remains unknown but the combination of various risk factors is involved. Two non mutually exclusive concepts have been suggested: one based on the primary contribution of T cells and the other on that of mesench...

journal_title：Annales de biologie clinique

authors： Miossec P

更新日期：1997-07-01 00:00:00

abstract：:Blood histamine proved to be important for the immune homeostasis. By its direct action or by means of H-2 receptors it end or prevents the excessive immune response. Recent studies revealed changes in cell mediated immunity in subjects with atopia, with increased histaminemia and their obvious protection against canc...

journal_title：Annales de biologie clinique

authors： Stanciu L

更新日期：1990-01-01 00:00:00

abstract：:HTLV genomic and antigenic features, replication way as well as associated pathology are recalled herein. The epidemiologic angle and the different transmission ways are also related. HTLV infection diagnostic implements are detailed: screening and specially confirmatory tests are brought to light with the help of con...

journal_title：Annales de biologie clinique

authors： Maniez-Montreuil M

更新日期：1998-09-01 00:00:00

abstract：:The evidence for disturbances of tryptophan and 5-hydroxytryptamine in depression and for various mechanisms by which such disturbances could occur is discussed. Two recent relevant studies in the author's laboratory are described: a) non-esterified fatty acid and total and free tryptophan were determined in plasmas o...

journal_title：Annales de biologie clinique

authors： Curzon G

更新日期：1979-01-01 00:00:00

abstract：:Various coagulation abnormalities were reported in HIV-infected patients. Cases of severe thrombocytopenia were first observed in contaminated homosexual males, as well as prolonged APTT due to the presence of lupus-like anticoagulant with a frequency in the range 8 to 70% of the studied patients. Even if lupus antico...

journal_title：Annales de biologie clinique

authors： Toulon P

更新日期：1998-03-01 00:00:00

abstract：:AFP can be measured by radioimmunoassay in 52 hours using an incubation at 18 degrees C for 48 hours and the double antibody solid phase method to separate the free labelled AFP from the labelled AFP bound to the antibodies. In these conditions, the sensitivity and precision of the assay are very satisfactory and the ...

journal_title：Annales de biologie clinique

authors： Franchimont P,Zangerle PF,Debruche ML,Proyard J,Simon M,Gaspard U

更新日期：1975-01-01 00:00:00

abstract：:The authors propose a modification of the method of use of the API 20E system permitting more rapid identification of Enterobacteriaceae within six hours (3 hours preculture and 3 hours incubation on an API 20E plate) it was possible to identify correctly 67% of 192 strains studied at species level and 75.5% studied a...

journal_title：Annales de biologie clinique

authors： Pinon G,Loulergue J,Quentin R,Darchis JP,Prieur D,Vargues R

更新日期：1979-01-01 00:00:00

abstract：:Schistocytes result from red cell fragmentation. The identification of the schistocytes is critical for appropriate management of the patients. We report the results of a survey about the determination of schistocytes. The analysis of the answers of 24 well-trained biologists points out the different approaches used t...

journal_title：Annales de biologie clinique

authors： Lesesve JF,Salignac S,Lecompte T

更新日期：2001-01-01 00:00:00

abstract：:We describe the case of a serological reactivation in a Toxoplasma-seropositive subject, following a cardiac transplantation transmitting cysts contained in the myocardial tissue. In a context of acute graft rejection, primary chemoprophylaxis enables to avoid onset of opportunistic toxoplasmosis, emerging with immuno...

journal_title：Annales de biologie clinique

authors： Desoubeaux G,Perret-Gallix K,Machet MC,Sirinelli A,Bailly E,Van Langendonck N,Chandenier J

更新日期：2012-05-01 00:00:00

abstract：:In biochemistry laboratory, monoclonal immunoglobulins are frequently found. They can lead to analytical interferences which are difficult to anticipate. In this case reports, a monoclonal immunoglobulin M with cryoglobulin effect has been responsible of false result of protein electrophoresis because of the whole pre...

journal_title：Annales de biologie clinique

authors： Oliver M,Ragot C,Savini H,Chianéa D,Navarro L,Moalic JL

更新日期：2007-09-01 00:00:00

abstract：:Monoclonal antibodies are being used as drugs in the diagnosis, prevention, and treatment of human diseases. The discipline of antibody engineering has evolved with modern cellular and molecular biology methods to meet the challenge of producing human monoclonal antibodies for in vivo biotherapy. In this overview, app...

journal_title：Annales de biologie clinique

authors： Hamilton RG

更新日期：1989-01-01 00:00:00

abstract：:Attention has been drawn in recent years to the diagnostic interest offered by increases in serum gamma GT in the detection of liver metastases and various cancers in man. Although this analysis is not specific and presents a few false positive and negative results, it seems however profitable to include it either in ...

journal_title：Annales de biologie clinique

authors： Braun JP,Soula G,Rico AG,Benard P,Burgat-Sacaze V

更新日期：1981-01-01 00:00:00

abstract：:The recent development of specific and sensitive biochemical markers reflecting the overall rate of bone formation and bone resorption, has markedly improved the non invasive assessment of bone turnover in various metabolic bone diseases, especially for osteoporosis. The immunoassay of human osteocalcin recognizing th...

journal_title：Annales de biologie clinique

authors： Garnero P,Delmas PD

更新日期：1999-03-01 00:00:00

abstract：:Determination of plasma delta-aminolaevulinic acid levels is of interest for the diagnosis of disorders of heme biosynthesis. We describe a reliable method for the determination of this parameter, based on a modification of the original method of Mauzerall and Granick. Analytical criteria are defined and the reference...

journal_title：Annales de biologie clinique

authors： Goujon R,Philibert E,Bernard P,Normand J,Boucherat M

更新日期：1992-01-01 00:00:00