Abstract:
:The authors describe a method of fractionation of urinary 17-oxosteroids after purification of the urinary extract by Girard's T reagent. After solvolysis, enzyme hydrolysis and purification by Girard's T reagent, the 17-oxosteroids were estimated in the form of trimethyl silyl ethers by gas liquid chromatographye on OV 225 using cholesterol acetate for standardisation. The improvement in the quality of the fraction using this technic, the analytical characteristics of which are studied in this work, is of definite interest, in particular after stimulation by ACTH, HCG, metopirone and in the case of pigmentation of the urine.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Habrioux Gsubject
Has Abstractpub_date
1978-01-01 00:00:00pages
433-42issue
5eissn
0003-3898issn
1950-6112journal_volume
36pub_type
杂志文章abstract:UNLABELLED:The aim of our study was to evaluate the sensitivity, specificity and predictive values of isolated and combined ASCA and NANA assays in Tunisian inflammatory bowel diseases patients and to assess whether their presence is associated with particular clinical features of the disease. METHODS:128 patients (39...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2010.0471
更新日期:2010-09-01 00:00:00
abstract::The great diversity of methods for manganese analysis in biological materials (serum, plasma, whole blood, urine, spinal fluid and hair) reveals the difficulty in measuring extremely small quantities of this element. An examination of these methods demonstrate that the most used one is flameless atomic absorption spec...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1988-01-01 00:00:00
abstract::Con A stimulated lymphocytes from some patients with primary glomerular disease release in vitro a lymphokine, the vascular permeability factor (VPF). House dust sensitization was early reported among these patients. We therefore studied the action of this specific mitogen on VPF production in 58 patients with primary...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Mucopolysaccharidosis type IV A (MPS IV A) is an autosomal recessive disorder resulting from the deficient activity of the lysosomal enzyme, N-acetylgalactosamine-6-sulfate sulfatase (GALNS) and the progressive lysosomal accumulation of keratane sulfate. Clinically, the MPS IV A differs from the other MPS by the local...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Eighteen paraplegics with or without neurogenic osteo-arthropathy (POAN) were evaluated for variations in their urinary excretion levels of total (T), and free (L) hydroxyproline and its non-dialysable (ND) and dialysable (D) peptide fractions. Increases in urinary hydroxyproline, moderate rises in free hydroxyproline...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2002-11-01 00:00:00
abstract::The authors describe a method of simultaneous estimation of phenobarbitone, mephenytoin and diphenylhydantoin in the blood. After extraction with ether, the methylation of these substances by trimethylanilinium hydroxide permits their rapid separation by gas chromatography. Heptabarbitone is used as internal standard ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Attention has been drawn in recent years to the diagnostic interest offered by increases in serum gamma GT in the detection of liver metastases and various cancers in man. Although this analysis is not specific and presents a few false positive and negative results, it seems however profitable to include it either in ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1981-01-01 00:00:00
abstract::The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::We describe the case of a serological reactivation in a Toxoplasma-seropositive subject, following a cardiac transplantation transmitting cysts contained in the myocardial tissue. In a context of acute graft rejection, primary chemoprophylaxis enables to avoid onset of opportunistic toxoplasmosis, emerging with immuno...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2012.0706
更新日期:2012-05-01 00:00:00
abstract::French medical laboratories must be accredited before November 2016 according to NF/EN/ISO 15189 standard. However, technical accreditation guidelines cannot be applied literally for the determination of specific IgE for several reasons: more than 600 allergen tests, lack of international gold standard, limited extern...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0830
更新日期:2013-05-01 00:00:00
abstract::Direct and indirect ion selective electrodes (ISEs) are two methods commonly used in biochemistry laboratories in order to measure the electrolytes such as sodium. In the clinical practice, it's the sodium concentration in plasma water -measured by direct ISE- which is important to consider as it is responsible of wat...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2015.1046
更新日期:2015-05-01 00:00:00
abstract::The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2017.1283
更新日期:2017-12-01 00:00:00
abstract::Cardiac troponin T (troponin Tc) of second generation has been measured on the Boehringer Elecsys 2010 analyzer. Cardiac specificity has been studied in patients presenting a rhabdomyolysis syndrome and the results compared with those obtained for cardiac troponin I (troponin Ic) measured on the Dade-Behring Stratus a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Among myeloproliferative disorders, Essential Thrombocythaemia is the most frequent and the one associated with the better prognosis. Although median survival is above 15 years, Essential Thrombocythaemia may be complicated by haemostatic life-threatening manifestations. Occurrence of thromboembolic or haemorrhagic ep...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2004-05-01 00:00:00
abstract::Serum level monitoring of drugs allows an optimal drug therapy, because it can take into account individual pharmacokinetic (and metabolic) variations. Some special conditions in drugs and in patients are highly suggestive of drug monitoring. These include mainly physiological but also pharmacological situations and a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1986-01-01 00:00:00
abstract::Glycated hemoglobin can be assayed by methods using different principles, whose performances vary and are difficult to control. The most widely used techniques measure either specifically glycated A1c hemoglobin (HbA1c) or total glycated hemoglobin. They are reviewed in detail in this paper and standardization problem...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1994-01-01 00:00:00
abstract::Determination of plasma delta-aminolaevulinic acid levels is of interest for the diagnosis of disorders of heme biosynthesis. We describe a reliable method for the determination of this parameter, based on a modification of the original method of Mauzerall and Granick. Analytical criteria are defined and the reference...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Panton-Valentine leukocidin-producing (PVL) Staphylococcus aureus is responsible for a highly lethal necrotizing pneumonia, which occurs predominantly in young immunocompetent patients. Hemoptysis and leucopenia often occur but are not always present. Detection of PVL gene on S. aureus strains responsible for pneumoni...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::The authors propose a modification of the method of use of the API 20E system permitting more rapid identification of Enterobacteriaceae within six hours (3 hours preculture and 3 hours incubation on an API 20E plate) it was possible to identify correctly 67% of 192 strains studied at species level and 75.5% studied a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::A recently commercialized dot-blot (Cyto-Dot, BMD) offered a new method for the detection of three autoantibodies (Ab) anti-Jo-1, anti-M2, and anti-ribosomal protein although their only common point is the cytoplasmic localisation of their respective antigen. These Ab are detected by indirect immunofluorescence (IF) (...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Diagnosis of systemic candidiasis is difficult and often performed lately. It can be improved by Candida antigen detection, using agglutination of latex particles sensitized with anti-Candida albicans antibody. Candida antigen search was made in 407 sera obtained from 123 patients, in Hematology and in intensive care ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Our data suggest that the hyperhomocysteinemia and/or increased plasma level of lipoprotein Lp(a) are risk factors for coronary heart disease. We investigated 178 patients who underwent complete cardiac examination comprising coronary angiography and biological analysis (CT, HDL-c, LDL-c, TG, and apoAI, apoB, homocyst...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Cystatin C is a low molecular weight-protein, which may replace creatinine for the evaluation of renal function, particularly in the clinical settings where the relationship between creatinine production and muscular mass impairs the clinical performance of creatinine. This paper intends to summarize the current knowl...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2008.0227
更新日期:2008-05-01 00:00:00
abstract::The multimeric glycoprotein Von Willebrand factor (vWF), is produced by vascular endothelium and platelet. If some constitutionals deficiencies leading to hemorrhagic syndrome have been explored in the literature, increased production of vWF observed during cellular distress and pregnancy have not been explored in our...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.0956
更新日期:2014-05-01 00:00:00
abstract::The identification of a product absorbed by an opiate consumer is sometimes problematic since there is no specific biomarker for all molecules. We developed an ultra-high pressure liquid chromatography coupled to tandem mass spectrometry technique which allows the identification and the quantification of 25 opiates in...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.0944
更新日期:2014-03-01 00:00:00
abstract::Lungs can be the target of many fungal parasitic organisms. Some of them are specific to the lung (Pneumocystis); while others will migrate there in the course of their life cycle (Ascaris) or be caught in it (Paragonimus, Echinococcus). Sometimes, they also generate hypersensitivity reactions (fungi). In making a dia...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1990-01-01 00:00:00
abstract::Increased level of C reactive protein in the serum of neonates indicates the presence of infection, whereas the classic signs (hyperthermia, neutrophilia...) may often fail. So the determination of C reactive protein is useful and has to be rapidly performed on a small quantity of blood. The authors evaluate a fluores...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::We propose a double antibody radioimmunoassay for human prostatic acid phosphatase (PAP) in serum for diagnosis and management of prostatic adenocarcinoma under treatment. The antigen is purified from human prostatic fluid by a gel-filtration on Sephadex G 100 followed by affinity chromatography on Con A Sepharose. A ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00