Abstract:
:A previous multicentric study set up by the Société française de biologie clinique has emphasized the usefulness of a standardized procedure for the determination by high performance liquid chromatography of alpha-tocopherol in serum or plasma. In our study, we have tested every step of the different published procedures: internal standard adduct, lipoprotein denaturation and vitamin extraction. Reproducibility of results was improved by the use of tocol as an internal standard when compared to retinol or alpha-tocopherol acetates. Lipoprotein denaturation was more efficient with ethanol addition than with methanol and when the ethanol/water ratio was > or = 0.7. Use of n-hexane or n-heptane gave the same recovery of alpha-tocopherol. When organic solvent/water ratio was > or = 1, n-hexane enabled to efficiently extract, in a one-step procedure, the alpha-tocopherol from both normo and hyperlipidemic sera. Performances of the selected procedure were: detection limit: 0.5 microM--linear range: 750 microM--within run coefficient of variation: 2.03%--day to day: 4.76%. Finally, this pluricentric study allows us to propose an optimised procedure for the determination of alpha-tocopherol in serum or plasma.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Jezequel-Cuer M,Le Moël G,Mounié J,Peynet J,Le Bizec C,Vernet MH,Artur Y,Laschi-Loquerie A,Troupel Ssubject
Has Abstractpub_date
1995-01-01 00:00:00pages
343-52issue
6eissn
0003-3898issn
1950-6112journal_volume
53pub_type
杂志文章,评审abstract::We present the distribution of cytochrome P450, the terminal oxidase of the microsomal drug-metabolizing system among the different species, organs, and its subcellular localization. Physicochemical properties, and more specially spectral properties, of this hemoprotein are reviewed. We also described the two microsom...
journal_title:Annales de biologie clinique
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abstract::Exploring coagulation in newborns and children is a challenge due to the low levels of both procoagulant factors and inhibitors. Conventional coagulation tests might be inadequate to explore all of these changes. The aim of the study is to evaluate the thrombin generation assay, a global test to explore coagulation, i...
journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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abstract::There has been rapid progress in techniques for the detection of antinuclear antibodies. The methods most widely used at present are indirect immunofluorescence, Ouchterlony double immunodiffusion, passive haemagglutination and the Farr test. Antinuclear antibodies recognise several antigens. With a few exceptions, in...
journal_title:Annales de biologie clinique
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更新日期:1982-01-01 00:00:00
abstract::We evaluated a rapid and semi-quantitative C-Reactive Protein test on whole blood, the Actim CRP (Fumouze). Based on immuno-chromatography technology, this test ranked the blood sample in four groups: < 10 mg/L, 10-40 mg/L, 40-80 mg/L and > 80 mg/L. This evaluation finds an excellent repeatability, the absence of hook...
journal_title:Annales de biologie clinique
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abstract::The discovery of free fetal DNA in the maternal circulation has inaugurated the era of non-invasive prenatal diagnosis. The latter has the advantage of avoiding the use of conventional obstetric procedures, such as chorionic villus sampling or aspiration of amniotic fluid, thus limiting the risks of miscarriage they i...
journal_title:Annales de biologie clinique
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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更新日期:1989-01-01 00:00:00
abstract::Legislation and ISO 15189 international standard provide that the clinical laboratories shall organize the communication of the biological assay results, especially with the establishment of a list of critical assays, whose results shall be reported during doctor's on call period but also through restricting result tr...
journal_title:Annales de biologie clinique
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doi:10.1684/abc.2017.1245
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abstract::The in vitro activity of the broad-spectrum antifungals miconazole, ketoconazole and itraconazole was evaluated by the decimal dilution method in liquid media, for respectively 2511, 1536 and 1859 strains of yeasts belonging to 31 species. Sabouraud broth was used for miconazole and brain heart infusion broth for keto...
journal_title:Annales de biologie clinique
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doi:
更新日期:1987-01-01 00:00:00
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journal_title:Annales de biologie clinique
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更新日期:1992-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Activation of plasminogen by tissue-type plasminogen activator (tpA) is potentiated by fibrin. We have demonstrated the role of fibrin polymerization in the potentiating effect of tpA-induced fibrinolysis. Therefore a pathogenic mechanism of thrombotic disorder may be related to an abnormal fibrin polymerization: the ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Premature rupture of the membranes (PROM) is a frequent event affecting 3% of pregnancies. PROM causes 30% of premature deliveries and 20% of perinatal mortality. The diagnosis relies mainly on the clinical visualization of the amniotic fluid flow in the vagina. If not, clinicians can use bedside tests detecting eithe...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2018.1346
更新日期:2018-06-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2008.0232
更新日期:2008-07-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Blood gas analyzers are able to provide information on a wide range of oxygenation parameters. Some of these data are complex. Their biological significance or clinical consistency are not always well known. This article aims to define these parameters, their reference values according to the type of sample; and to ou...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2017.1274
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2009.0309
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abstract::Apolipoprotein B (apoB) is the major proteic component of LDL, VLDL and chylomicrons. Numerous polymorphisms of the apolipoprotein B gene have been described. Particularly, a polymorphism of insertion/deletion located in the coding part of the signal peptide of apoB, associated with modifications of lipid concentratio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The analysis of longitudinal series of tumour markers during chemotherapy in patients with germ cells tumors is not easy. The purpose of this work is to present various characteristic profiles of the evolution of serum human chorionic gonadotropin during chemotherapy and review the modalities of the dynamic interpreta...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2008.0260
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The purpose of this study is to find, besides the determination of lactate in blood, a suitable and specific indicator of brain damage in newborn. Accordingly, we studied the total creatine kinase (CK) activity, by the method of Rosalki, and its subunit isoenzymes BB (CK BB) by fluorescence following electrophoresis o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Serum level monitoring of drugs allows an optimal drug therapy, because it can take into account individual pharmacokinetic (and metabolic) variations. Some special conditions in drugs and in patients are highly suggestive of drug monitoring. These include mainly physiological but also pharmacological situations and a...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1986-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::ATB 32 GN was tested with 279 gram negative non fastidious rods = 188 Enterobacteriaceae and 91 non-enterobacteriaceae. This micro-method included 32 carbon substrate's assimilation tests, read and interpreted automatically after incubation for one or two days at 30 degrees C. 93.5 p. cent of all bacteria are correctl...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
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abstract::Multidrug resistance (MDR) phenotype expression was evaluated retrospectively in 87 patients with acute myeloid leukemia (AML), 69 with de novo AML, ten with relapsed AML and eight with AML secondary to myelodysplastic syndrome (MDS). MDR phenotype, characterized by P-glycoprotein expression (MRK16 monoclonal antibody...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00