Quantification of red blood cell fragmentation: usefulness of heating cells and of automatic counting devices.

Abstract:

:Spontaneous red blood cell (RBC) fragmentation occurs in some membrane erythropathies like hereditary elliptocytosis (HE); this phenomenon is produced in normal RBC by heating at 49 degrees C, but not at temperatures below this limit; fragmentation is usually quantified by counting the number of fragments/1000 RBC under light microscopic examination. The present work demonstrates: i) that enumeration of fragments is performed more precisely with an automatic blood cells counter on the 'platelet' channel; and ii) that heating at 48 degrees C enhances the fragmentation of RBC when they have a severe disruption of skeletal lattice, like in HE.

journal_name

Ann Biol Clin (Paris)

authors

Guetarni D,Zerhouni F,Beldjord K,Henni T,Godet J,Colonna P

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

649-51

issue

9

eissn

0003-3898

issn

1950-6112

journal_volume

50

pub_type

杂志文章
  • [Germ cell testicular tumors: understanding the kinetics of serum human chorionic gonadotropin during chemotherapy].

    abstract::The analysis of longitudinal series of tumour markers during chemotherapy in patients with germ cells tumors is not easy. The purpose of this work is to present various characteristic profiles of the evolution of serum human chorionic gonadotropin during chemotherapy and review the modalities of the dynamic interpreta...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2008.0260

    authors: Riedinger JM,Eche N,Fargeot P

    更新日期:2008-09-01 00:00:00

  • [Isolation, identification and clinical significance of species of the Flavobacterium genus].

    abstract::Strictly aerobic Gram negative bacteria are found more and more often in human pathological specimens and in the environment. Amongst these bacteria, Flavobacterium poses problems of bacteriological diagnosis as their precise taxonomy is still recent. In this study, conducted on 321 strains of Flavobacterium from vari...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Richard C,Monteil H

    更新日期:1983-01-01 00:00:00

  • [A critical study of the screening practices of gestational diabetes of a service of gynecology and obstetrics].

    abstract::Our study has for aim to describe the practices of screening of the gestational diabetes mellitus. Screening test was realised at 61% of the women included in the study. The HGPO in 50 g was prescribed in 47% of the cases, a single fasting blood glucose in 28% of the cases; 13,4% of the detected women had a glycaemia ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2009.0309

    authors: Bouhsain S,Dami A,Elannaz H,Guelzim K,Baba H,Elhassani MM,Mechtani S,Tellal S,Dhayni M,Moussaoui D,Derouiche M

    更新日期:2009-03-01 00:00:00

  • [Hemolysis interferences on frequently required stat analysis: a French multicentric study].

    abstract::Hemolysis should lead to changes in test results. Our study evaluated the impact of hemolysis on 26 blood measurements of stat biochemistry markers (sodium, potassium, chloride, urea, creatinine, glucose, total protein, calcium, magnesium, inorganic phosphorus, uric acid, C-reactive protein, total bilirubin, ASAT, ALA...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2015.1090

    authors: Poupon C,Lefèvre G,Ngo-François S,Alibeu C,Barbé F,Bourbonneux V,Cartier R,Morin C,Szymanowicz A,Vuillaume I,Groupe de travail du Collège national de biochimie des hôpitaux.

    更新日期:2015-11-01 00:00:00

  • [Evaluation of transcutaneous bilirubin measurement and agreement with bilirubinemia].

    abstract::Evaluation of the neonates for jaundice and kernicterus is indispensable when early hospital discharge has become standard practice. Transcutaneous bilirubin (TcB) measurement is an advantageous option because of its non-invasive nature and the possibility of its use near the patient. The point of care device BiliChec...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Carceller AM,Delvin E,Gonthier M,Grégoire MC,Cousineau J,Alexandrov L

    更新日期:2006-11-01 00:00:00

  • [Circulating citrulline levels: a biomarker for intestinal functionality assessment].

    abstract::Circulating citrulline is emerging as an innovating biomarker candidate for assessment of intestinal function. Amino acid synthesized by enterocytes of intestinal mucosal, citrulline is not included in proteins or nutrition products and is a precursor for production of arginine by the kidney. Plasma citrulline is, in ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:10.1684/abc.2011.0609

    authors: Crenn P,Hanachi M,Neveux N,Cynober L

    更新日期:2011-09-01 00:00:00

  • [The new HemoCue system Hb 301 for the haemoglobin measurement in pregnant women].

    abstract::The performance of the HemoCue apparatus in the diagnosis of anaemia in pregnancy was evaluated using the HemoCue Hb 301 and the HemoCue Hb 201+ apparatus. The HemoCue Hb 201 + was considered as the reference method in this study. Two hundred and thirty six pregnant women attending the University Teaching Hospital in ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2008.0195

    authors: Tayou Tagny C,Kouam L,Mbanya D

    更新日期:2008-01-01 00:00:00

  • Hemolytic anemia, iron deficiency and personal history of deep vein thrombosis: consider paroxysmal nocturnal hemoglobinuria.

    abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired hematopoietic stem cell disorder that must be correctly diagnose because it is a chronic disease with a real impact on the quality of life and the survival of the patients. PNH screening of all patients with anemia or thrombosis is not recommended. We report...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2017.1277

    authors: Gendron N,Arlet JB,Gaussem P,Radford-Weiss I,Dupeux S,Rosain J,La Tour RP,Darnige L

    更新日期:2017-10-01 00:00:00

  • [An uncommon etiology of anemia: copper deficiency].

    abstract::A 58-year-old patient, without any notable medical history, except for alcoholism and treated hypertension, developed anemia and leukopenia with macrocytosis. Folate deficiency was diagnosed and subsequently treated. Despite folate supplementation, the hematological parameters did not normalize. Further diagnosis inve...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2013.0863

    authors: Kouamou E,Stépanian A,Khadra F,de Prost D,Teillet F

    更新日期:2013-07-01 00:00:00

  • [Practical aspects of leukotriene E4 determination in urine].

    abstract::Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and rad...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Chabane MH,Le Vraux V,Le Provost AM,Leynadier F

    更新日期:1995-01-01 00:00:00

  • 24 hours calciuria: normal values, regulations: about a cohort of 317 control subjects.

    abstract::In a control population, we filed the 24-hour urinary calcium to set normal values, based on weight, BMI and menopause. By assessing calcium intake, 25OHD, PTH, CTX, GFR, we wanted to study how these could influence calcium excretion. A total of 317 subjects of 55.82 ± 12.6 years were studied: 249 women (210 were post...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2016.1174

    authors: Laroche M,Nigon D,Gennero I,Lassoued S,Pouilles JM,Trémolières F,Vallet M,Tack I

    更新日期:2016-08-01 00:00:00

  • Protective effect of ginger against toxicity induced by chromate in rats.

    abstract::The evaluation of the effect of ginger on the modulation of toxic effects induced by chromate is the objective of our study. 50 male rats Albinos Wistar were divided to five groups as follow: group I (T) is served as control, received a mineral water by gavage (per os); group II (G) received an experimental diet with ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2013.0806

    authors: Krim M,Messaadia A,Maidi I,Aouacheri O,Saka S

    更新日期:2013-03-01 00:00:00

  • Study of disturbed tryptophan metabolism in depressive illness.

    abstract::The evidence for disturbances of tryptophan and 5-hydroxytryptamine in depression and for various mechanisms by which such disturbances could occur is discussed. Two recent relevant studies in the author's laboratory are described: a) non-esterified fatty acid and total and free tryptophan were determined in plasmas o...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Curzon G

    更新日期:1979-01-01 00:00:00

  • Cryopreservation of in vitro cultured mouse preimplantation embryos.

    abstract::We investigated two different freezing protocols on mouse embryos that were either grown in vivo or were grown in vitro for a certain period. Our results confirm that an extended in vitro culture period makes the embryo more susceptible for injury due to freezing and thawing. Furthermore when freezing two cell mouse e...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Van den Abbeel E,Van Steirteghem AC

    更新日期:1987-01-01 00:00:00

  • [In vitro effect of Hordeum vulgare on the crystallization of calcium oxalate monohydrate (whewellite)].

    abstract::The recommended conservative treatment of hyperoxaluria is mainly based on hyperhydration and ingestion of inhibitors of crystallization. In accordance with this context, the aim of this study was to determine the in vitro effect of Hordeum vulgare on calcium oxalate crystallization oxalo-dependent. The crystallizatio...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2012.0770

    authors: Djaroud S,Harrache D,Amar A

    更新日期:2012-11-01 00:00:00

  • [Prescribing guidance pocket chart on the initiation of warfarin therapy in a geriatric hospital: a useful but challenging tool].

    abstract::Elderly patients are at high risk of over-anticoagulation and of haemorrhagic risk when on warfarin, especially during treatment induction. In Charles Foix Hospital, a 800-bed geriatric hospital, we specifically developed for in-patients older than 70 years (target INR 2.5) a simple low-dose warfarin induction regimen...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Siguret V,N'Guyen A,Harboun M,Chevallier A,Pautas E,Gouin-Thibault I,Berigaud S

    更新日期:2006-05-01 00:00:00

  • Myoadenylate deaminase deficiency: a frequent cause of muscle pain A case detected by exercise testing.

    abstract::Myoadenylate deaminase deficit (MAD, MIM#615511) is the most common cause of metabolic myopathies with an estimated prevalence of 1-2% in the general population. We report the case of a 39-year-old man suffering from severe skeletal muscle pain that had developed gradually for 4 years. A moderate increase in creatine ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2017.1253

    authors: Lim L,Palayer M,Bruneau A,Letournel F,Le Maréchal C,Simard G,Reynier P,Homedan C,Nadaj-Pakleza A

    更新日期:2017-08-01 00:00:00

  • Magazines in waiting areas of hospital: a forgotten microbial reservoir?

    abstract::The hospital environment is a potential source of microbial contamination. Thus, the magazines in hospital's waiting rooms are handled by patients and visitors whose health and hygiene conditions can vary widely. In this context, we had measured the microbial load on the surface of magazines. Fifteen magazines from 5 ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2017.1283

    authors: Adé M,Burger S,Cuntzmann A,Exinger J,Meunier O

    更新日期:2017-12-01 00:00:00

  • [Microsomal epoxide hydrolase (author's transl)].

    abstract::Microsomal epoxide hydrolase (EC 3.3.2.3. ; formerly EC 4.2.1.63) catalyse the hydration of epoxides into trans dihydro-diols, easily excretable hydrophil compounds. It is an enzyme with very wide specificity for monosubstituted oxiranes, or cis disubstituted oxiranes. It is inactive on polysubstituted epoxides. The h...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:

    authors: Dansette PM

    更新日期:1980-01-01 00:00:00

  • [Study of Urinary excretion of total, free, and non-dialysable conjugated hydroxyproline in paraplegics].

    abstract::Eighteen paraplegics with or without neurogenic osteo-arthropathy (POAN) were evaluated for variations in their urinary excretion levels of total (T), and free (L) hydroxyproline and its non-dialysable (ND) and dialysable (D) peptide fractions. Increases in urinary hydroxyproline, moderate rises in free hydroxyproline...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Pilonchery G,Finck C,Minaire P,Messy P,Revol A

    更新日期:1982-01-01 00:00:00

  • [Biochemical markers of bone turnover : clinical usefulness in osteoporosis].

    abstract::The recent development of specific and sensitive biochemical markers reflecting the overall rate of bone formation and bone resorption, has markedly improved the non invasive assessment of bone turnover in various metabolic bone diseases, especially for osteoporosis. The immunoassay of human osteocalcin recognizing th...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:

    authors: Garnero P,Delmas PD

    更新日期:1999-03-01 00:00:00

  • [Evaluation of the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie].

    abstract::We have evaluated the methodological quality of the Rémic (microbiology guidelines - bacteriology and mycology) of the Société française de microbiologie (edition2007), using to AGREE criteria, which are consensual at an international level, in particular at the the World Health Organisation (WHO) and at the European ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2011.0536

    authors: Fonfrède M,Couaillac JP,Augereau C,De Moüy D,Lepargneur JP,Szymanowicz A,Watine J

    更新日期:2011-03-01 00:00:00

  • [Comparative study of G8 glycated haemoglobin testing system and the Integra 800 immunoassay].

    abstract::We compared the results of two methods of measurement of HbA(1c) before replacing the current method on Integra 800 by the HPLC method on an apparatus recently put on the market, the G8. The comparative study of the results for 119 patients was carried out. The reproducibility obtained with the G8 method is good with ...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:10.1684/abc.2010.0420

    authors: Szymanowicz A,Neyron MJ

    更新日期:2010-03-01 00:00:00

  • [Blood sampling using "dried blood spot": a clinical biology revolution underway?].

    abstract::Blood testing using the dried blood spot (DBS) is used since the 1960s in clinical analysis, mainly within the framework of the neonatal screening (Guthrie test). Since then numerous analytes such as nucleic acids, small molecules or lipids, were successfully measured on the DBS. While this pre-analytical method repre...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:10.1684/abc.2014.1007

    authors: Hirtz C,Lehmann S

    更新日期:2015-01-01 00:00:00

  • [SFBC guidelines on critical care testing].

    abstract::SFBC working group on critical care testing describes in this paper guideline for the management of laboratory medicine examination process in emergency conditions. After a summary on French standards and regulations, the critical care testing perimeter and definitions of stat levels are presented in different context...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,实务指引

    doi:10.1684/abc.2015.1113

    authors: Vaubourdolle M,Alvarez JC,Barbé F,Beaudeux JL,Boissier É,Caillon H,Chatron P,Joly-Guillou ML,Mailloux A

    更新日期:2016-03-01 00:00:00

  • [Assay of cardiac troponin T on Elecsys 2010: comparison with cardiac troponin I].

    abstract::Cardiac troponin T (troponin Tc) of second generation has been measured on the Boehringer Elecsys 2010 analyzer. Cardiac specificity has been studied in patients presenting a rhabdomyolysis syndrome and the results compared with those obtained for cardiac troponin I (troponin Ic) measured on the Dade-Behring Stratus a...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Cauliez B,Hue G,Lavoinne A

    更新日期:1998-11-01 00:00:00

  • [Pulmonary mycoses and parasitosis].

    abstract::Lungs can be the target of many fungal parasitic organisms. Some of them are specific to the lung (Pneumocystis); while others will migrate there in the course of their life cycle (Ascaris) or be caught in it (Paragonimus, Echinococcus). Sometimes, they also generate hypersensitivity reactions (fungi). In making a dia...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:

    authors: De Gentile L,Chabasse D

    更新日期:1990-01-01 00:00:00

  • [Isoelectrofocusing in the study of the heterogeneity of immunoglobulins G in man with or without proliferative symptomatology].

    abstract::In the present investigation, the immunoglobulins G of human sera were studied after isoelectrofocusing using polyacrylamide flat bed gel. The heterogeneity of immunoglobulins was demonstrated in normal serum as well as in pathological situations. Isoelectrofocusing study was performed using the total serum or the imm...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Chopin N,Lacour B,Thierry D,Faure A,Bailly M

    更新日期:1983-01-01 00:00:00

  • [Metabolic syndrome: pathophysiology and impact on lithogenesis].

    abstract::In our societies, the increase of the frequency of the diseases of overweight, in particular obesity, diabetes type 2 and metabolic syndrome, coincides with that of the urinary lithiasis. Like the lithiasic disease, the metabolic syndrome or syndrome X is multi-factor. Several epidemiological studies were interested i...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章,评审

    doi:10.1684/abc.2008.0187

    authors: Zerifi R,Bahlous A,Marakchi O,Daudon M,Bartagi Z,Abdelmoula J

    更新日期:2008-01-01 00:00:00

  • [Determination of plasma creatinine with centrifugal analyser].

    abstract::An automated kinetic method to determine creatinine in plasma with Jaffé reaction (alkaline picrate reagent) using a Gemsaec analyser is studied in order to argue the precision and the accuracy. The analysis of the reaction rate of a primar standard solution and of different plasmas suggests that a rapid measure is re...

    journal_title:Annales de biologie clinique

    pub_type: 杂志文章

    doi:

    authors: Klumpp T,Férard G

    更新日期:1977-01-01 00:00:00